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/**
* This class provides functions that are dependent of the alignmentSettings!
* For functions that are not dependent of any other HiLive class, please use the tools_static class!
* Please do NOT add further includes to this file since this will lead to unwanted dependencies!
*/
#ifndef TOOLS_H
#define TOOLS_H
/* DONT ADD ANY INCLUDES */
#include "tools_static.h"
#include "alignmentSettings.h"
#include "global_variables.h"
/* DONT ADD ANY INCLUDES */
//////////////////////////////////////
////////// Build file names //////////
//////////////////////////////////////
/**
* Get the name of a bcl file.
* @param ln The lane number.
* @param tl The tile number.
* @param cl The sequencing cycle.
* @return Path to the bcl file.
*/
inline std::string get_bcl_fname(uint16_t ln, uint16_t tl, uint16_t cl) {
std::ostringstream path_stream;
path_stream << globalAlignmentSettings.get_root() << "/L" << to_N_digits(ln,3) << "/C" << cl << ".1/s_"<< ln <<"_" << tl << ".bcl";
return path_stream.str();
}
/**
* Get the name of an alignment file.
* @param ln The lane number.
* @param tl The tile number.
* @param cl The cycle for the respective mate.
* @param mt The mate number.
* @return Path to the alignment file.
*/
inline std::string get_align_fname(uint16_t ln, uint16_t tl, uint16_t cl, uint16_t mt){
std::ostringstream path_stream;
std::string base = globalAlignmentSettings.get_temp_dir() != "" ? globalAlignmentSettings.get_temp_dir() : globalAlignmentSettings.get_root();
path_stream << base << "/L" << to_N_digits(ln,3) << "/s_"<< ln << "_" << tl << "." << mt << "."<< cl << ".align";
return path_stream.str();
}
/**
* Get the name of a filter file.
* @param ln The lane number.
* @param tl The tile number.
* @return Path to the filter file.
*/
inline std::string get_filter_fname(uint16_t ln, uint16_t tl) {
std::ostringstream path_stream;
path_stream << globalAlignmentSettings.get_root() << "/L" << to_N_digits(ln,3) << "/s_"<< ln << "_" << tl << ".filter";
return path_stream.str();
}
/**
* Get the name of a clocs file.
* @param ln The lane number.
* @param tl The tile number.
* @return Path to the clocs file.
*/
inline std::string get_clocs_fname(uint16_t ln, uint16_t tl) {
std::ostringstream path_stream;
path_stream << globalAlignmentSettings.get_root() << "../L" << to_N_digits(ln,3) << "/s_"<< ln << "_" << tl << ".clocs";
return path_stream.str();
}
/**
* Get the name of the settings file.
* @return Path to the settings file.
*/
inline std::string get_config_fname() {
std::ostringstream path_stream;
path_stream << globalAlignmentSettings.get_temp_dir() << "/hilive_config.ini";
return path_stream.str();
}
/** Get the current sequencing cycle using the current alignment cycle and read number.
* @param cycle The read cycle.
* @param seq_id The sequence id (:= id of the respective element in globalAlignmentSettings::seqs)
* @return The sequencing cycle.
* @author Tobias Loka
*/
inline uint16_t getSeqCycle(uint16_t cycle, uint16_t seq_id) {
uint16_t seq_cycle = cycle;
for ( int i = 0; i < seq_id; i++ )
seq_cycle += globalAlignmentSettings.get_seq_by_id(i).length;
return seq_cycle;
}
/**
* Get the cycle of a mate for a given sequencing cycle.
* When the mate is completely finished in the given cycle, return its total sequence length.
* @param mate_number Mate of interest.
* @param seq_cycle The sequencing cycle.
* @return Cycle of the mate in the given sequencing cycle.
* @author Tobias Loka
*/
inline uint16_t getMateCycle( uint16_t mate_number, uint16_t seq_cycle ) {
// Invalid mate
if ( mate_number == 0 || mate_number > globalAlignmentSettings.get_mates() )
return 0;
// Iterate through all sequence elements (including barcodes)
for ( CountType id = 0; id < globalAlignmentSettings.get_seqs().size(); id++ ) {
// Current sequence element
SequenceElement seq = globalAlignmentSettings.get_seq_by_id(id);
// Seq is mate of interest
if ( seq.mate == mate_number )
return ( seq.length > seq_cycle ? seq_cycle : seq.length );
// Not enough cycles left to reach mate of interest
else if ( seq.length >= seq_cycle )
return 0;
// Reduce number of cycles by the Seq length
else
seq_cycle -= seq.length;
}
// Should not be reached
return 0;
}
////////////////////////////////////
////////// SAM/BAM output //////////
////////////////////////////////////
/**
* Get the header for a SAM/BAM output file.
* @return The BAM header.
* @author Tobias Loka
*/
seqan2::BamHeader getBamHeader();
/**
* Name of a temporary SAM/BAM file (for the time it is written).
* @param barcode Barcode of the output file (or "undetermined" for undetermined reads)
* @param cycle The output cycle.
* @return Name of the temporary output file for writing.
* @author Tobias Loka
*/
inline std::string getBamTempFileName(std::string barcode, CountType cycle) {
std::ostringstream fname;
std::string file_suffix = globalAlignmentSettings.get_output_format() == OutputFormat::BAM ? ".bam" : ".sam";
fname << globalAlignmentSettings.get_out_dir() << "/hilive_out_" << "cycle" << std::to_string(cycle) << "_" << barcode << ".temp" << file_suffix;
return fname.str();
}
/**
* Final name of a SAM/BAM file.
* @param barcode Barcode of the output file (or "undetermined" for undetermined reads)
* @param cycle The output cycle.
* @return Name of the final output file.
* @author Tobias Loka
*/
inline std::string getBamFileName(std::string barcode, CountType cycle) {
std::ostringstream fname;
std::string file_suffix = globalAlignmentSettings.get_output_format() == OutputFormat::BAM ? ".bam" : ".sam";
fname << globalAlignmentSettings.get_out_dir() << "/hilive_out_" << "cycle" << std::to_string(cycle) << "_" << barcode << file_suffix;
return fname.str();
}
/** Reverse a MD:Z tag for reverse alignments. */
std::string reverse_mdz(std::string mdz);
/////////////////////////////
////////// Scoring //////////
/////////////////////////////
inline uint16_t getMinSingleErrorPenalty() {
// Mismatch: +1 mismatch, -1 match
uint16_t mismatch_penalty = globalAlignmentSettings.get_mismatch_penalty() + globalAlignmentSettings.get_match_score();
// Deletion: +1 deletion, maximum number of matches can still be reached
uint16_t deletion_penalty = globalAlignmentSettings.get_deletion_opening_penalty() + globalAlignmentSettings.get_deletion_extension_penalty();
// Insertion: +1 insertion, -1 match
uint16_t insertion_penalty = globalAlignmentSettings.get_insertion_opening_penalty() + globalAlignmentSettings.get_insertion_extension_penalty() + globalAlignmentSettings.get_match_score();
return std::min(mismatch_penalty, std::min(insertion_penalty, deletion_penalty));
}
inline uint16_t getMaxSingleErrorPenalty() {
// Mismatch: +1 mismatch, -1 match
uint16_t mismatch_penalty = globalAlignmentSettings.get_mismatch_penalty() + globalAlignmentSettings.get_match_score();
// Deletion: +1 deletion, maximum number of matches can still be reached
uint16_t deletion_penalty = globalAlignmentSettings.get_deletion_opening_penalty() + globalAlignmentSettings.get_deletion_extension_penalty();
// Insertion: +1 insertion, -1 match
uint16_t insertion_penalty = globalAlignmentSettings.get_insertion_opening_penalty() + globalAlignmentSettings.get_insertion_extension_penalty() + globalAlignmentSettings.get_match_score();
return std::max(mismatch_penalty, std::max(insertion_penalty, deletion_penalty));
}
inline ScoreType getMaxPossibleScore( CountType cycles ) {
return cycles * globalAlignmentSettings.get_match_score();
}
inline CountType getMinSoftclipPenalty( CountType softclip_length ) {
return ceil( float(softclip_length) / globalAlignmentSettings.get_anchor_length() ) * getMinSingleErrorPenalty();
}
inline ScoreType getMinCycleScore( CountType cycle, CountType read_length ) {
if ( cycle < globalAlignmentSettings.get_anchor_length() )
return globalAlignmentSettings.get_min_as();
ScoreType maxScore = getMaxPossibleScore(read_length);
ScoreType minCycleScore = maxScore - ( ceil((cycle - globalAlignmentSettings.get_anchor_length()) / float(globalAlignmentSettings.get_error_rate())) * getMinSingleErrorPenalty() );
return std::max(minCycleScore, globalAlignmentSettings.get_min_as());
}
/////////////////////////////////
////////// Other stuff //////////
/////////////////////////////////
/**
* Copy a file while locking them in the global fileLocks.
*/
int atomic_rename( const char *oldname, const char *newname );
/**
* Check if a cycle is a seeding cycle.
*/
inline bool isSeedingCycle(CountType cycle) {
// Don't seed cycles smaller than the anchor length
if ( cycle < globalAlignmentSettings.get_anchor_length() )
return false;
// Don't seed cycles if the maximal softclip length would be exceeded
if ( ( cycle - globalAlignmentSettings.get_anchor_length() ) > globalAlignmentSettings.get_max_softclip_length())
return false;
// Create seeds when reaching the anchor length for the first time
if ( cycle == globalAlignmentSettings.get_anchor_length() )
return true;
// Create seeds every seeding_interval cycle after the first anchor
if ( ( cycle - globalAlignmentSettings.get_anchor_length() ) % globalAlignmentSettings.get_seeding_interval() == 0 )
return true;
return false;
}
#endif /* TOOLS_H */
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