1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473 474 475 476 477 478 479 480 481 482 483 484 485 486 487 488 489 490 491 492 493 494 495 496 497 498 499 500 501 502 503 504 505 506 507 508 509 510 511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555 556 557 558 559 560 561 562 563 564 565 566 567 568 569 570 571 572 573 574 575 576 577 578 579 580 581 582 583 584 585 586 587 588 589 590 591 592 593 594 595 596 597 598 599 600 601 602 603 604 605 606 607 608 609 610 611 612 613 614 615 616 617 618 619 620 621 622 623 624 625 626 627 628 629 630 631 632 633 634 635 636 637 638 639 640 641 642 643 644 645 646 647 648 649 650 651 652 653 654 655 656 657 658 659 660 661 662 663 664 665 666 667 668 669 670 671 672 673 674 675 676 677 678 679 680 681 682 683 684 685 686 687 688 689 690 691 692 693 694 695 696 697 698 699 700 701 702 703 704 705 706 707 708 709 710 711 712 713 714 715 716 717 718 719 720 721 722 723 724 725 726 727 728 729 730 731 732 733 734 735 736 737 738 739 740 741 742 743 744 745 746 747 748 749 750 751 752 753 754 755 756 757 758 759 760 761 762 763 764 765 766 767 768 769 770 771 772 773 774 775 776 777 778 779 780 781 782 783 784 785
|
/*
* Copyright 2011, Ben Langmead <langmea@cs.jhu.edu>
*
* This file is part of Bowtie 2.
*
* Bowtie 2 is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* Bowtie 2 is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with Bowtie 2. If not, see <http://www.gnu.org/licenses/>.
*/
#include <iomanip>
#include <limits>
#include "aln_sink.h"
#include "aligner_seed.h"
#include "util.h"
using namespace std;
/**
* Initialize state machine with a new read. The state we start in depends
* on whether it's paired-end or unpaired.
*/
void ReportingState::nextRead(bool paired) {
paired_ = paired;
if(paired) {
state_ = CONCORDANT_PAIRS;
doneConcord_ = false;
doneDiscord_ = p_.discord ? false : true;
doneUnpair1_ = p_.mixed ? false : true;
doneUnpair2_ = p_.mixed ? false : true;
exitConcord_ = ReportingState::EXIT_DID_NOT_EXIT;
exitDiscord_ = p_.discord ?
ReportingState::EXIT_DID_NOT_EXIT :
ReportingState::EXIT_DID_NOT_ENTER;
exitUnpair1_ = p_.mixed ?
ReportingState::EXIT_DID_NOT_EXIT :
ReportingState::EXIT_DID_NOT_ENTER;
exitUnpair2_ = p_.mixed ?
ReportingState::EXIT_DID_NOT_EXIT :
ReportingState::EXIT_DID_NOT_ENTER;
} else {
// Unpaired
state_ = UNPAIRED;
doneConcord_ = true;
doneDiscord_ = true;
doneUnpair1_ = false;
doneUnpair2_ = true;
exitConcord_ = ReportingState::EXIT_DID_NOT_ENTER; // not relevant
exitDiscord_ = ReportingState::EXIT_DID_NOT_ENTER; // not relevant
exitUnpair1_ = ReportingState::EXIT_DID_NOT_EXIT;
exitUnpair2_ = ReportingState::EXIT_DID_NOT_ENTER; // not relevant
}
doneUnpair_ = doneUnpair1_ && doneUnpair2_;
done_ = false;
nconcord_ = ndiscord_ = nunpair1_ = nunpair2_ = 0;
nunpairRepeat1_ = nunpairRepeat2_ = 0;
concordBest_ = getMinScore();
}
/**
* Caller uses this member function to indicate that one additional
* concordant alignment has been found.
*/
bool ReportingState::foundConcordant(TAlScore score) {
assert(paired_);
assert_geq(state_, ReportingState::CONCORDANT_PAIRS);
assert(!doneConcord_);
if(score > concordBest_) {
concordBest_ = score;
nconcord_ = 0;
}
nconcord_++;
// DK CONCORDANT - debugging purpuses
// areDone(nconcord_, doneConcord_, exitConcord_);
// No need to search for discordant alignments if there are one or more
// concordant alignments.
doneDiscord_ = true;
exitDiscord_ = ReportingState::EXIT_SHORT_CIRCUIT_TRUMPED;
if(doneConcord_) {
// If we're finished looking for concordant alignments, do we have to
// continue on to search for unpaired alignments? Only if our exit
// from the concordant stage is EXIT_SHORT_CIRCUIT_M. If it's
// EXIT_SHORT_CIRCUIT_k or EXIT_WITH_ALIGNMENTS, we can skip unpaired.
assert_neq(ReportingState::EXIT_NO_ALIGNMENTS, exitConcord_);
if(exitConcord_ != ReportingState::EXIT_SHORT_CIRCUIT_M) {
if(!doneUnpair1_) {
doneUnpair1_ = true;
exitUnpair1_ = ReportingState::EXIT_SHORT_CIRCUIT_TRUMPED;
}
if(!doneUnpair2_) {
doneUnpair2_ = true;
exitUnpair2_ = ReportingState::EXIT_SHORT_CIRCUIT_TRUMPED;
}
}
}
updateDone();
return done();
}
/**
* Caller uses this member function to indicate that one additional unpaired
* mate alignment has been found for the specified mate.
*/
bool ReportingState::foundUnpaired(bool mate1, bool repeat) {
assert_gt(state_, ReportingState::NO_READ);
// Note: it's not right to assert !doneUnpair1_/!doneUnpair2_ here.
// Even if we're done with finding
if(mate1) {
nunpair1_++;
if(repeat) {
nunpairRepeat1_++;
}
// Did we just finish with this mate?
if(!doneUnpair1_) {
areDone(nunpair1_, doneUnpair1_, exitUnpair1_);
if(doneUnpair1_) {
doneUnpair_ = doneUnpair1_ && doneUnpair2_;
updateDone();
}
}
if(nunpair1_ > 1) {
doneDiscord_ = true;
exitDiscord_ = ReportingState::EXIT_NO_ALIGNMENTS;
}
} else {
nunpair2_++;
if(repeat) {
nunpairRepeat2_++;
}
// Did we just finish with this mate?
if(!doneUnpair2_) {
areDone(nunpair2_, doneUnpair2_, exitUnpair2_);
if(doneUnpair2_) {
doneUnpair_ = doneUnpair1_ && doneUnpair2_;
updateDone();
}
}
if(nunpair2_ > 1) {
doneDiscord_ = true;
exitDiscord_ = ReportingState::EXIT_NO_ALIGNMENTS;
}
}
return done();
}
/**
* Called to indicate that the aligner has finished searching for
* alignments. This gives us a chance to finalize our state.
*
* TODO: Keep track of short-circuiting information.
*/
void ReportingState::finish() {
if(!doneConcord_) {
doneConcord_ = true;
exitConcord_ =
((nconcord_ > 0) ?
ReportingState::EXIT_WITH_ALIGNMENTS :
ReportingState::EXIT_NO_ALIGNMENTS);
}
assert_gt(exitConcord_, EXIT_DID_NOT_EXIT);
if(!doneUnpair1_) {
doneUnpair1_ = true;
exitUnpair1_ =
((nunpair1_ > 0) ?
ReportingState::EXIT_WITH_ALIGNMENTS :
ReportingState::EXIT_NO_ALIGNMENTS);
}
assert_gt(exitUnpair1_, EXIT_DID_NOT_EXIT);
if(!doneUnpair2_) {
doneUnpair2_ = true;
exitUnpair2_ =
((nunpair2_ > 0) ?
ReportingState::EXIT_WITH_ALIGNMENTS :
ReportingState::EXIT_NO_ALIGNMENTS);
}
assert_gt(exitUnpair2_, EXIT_DID_NOT_EXIT);
if(!doneDiscord_) {
// Check if the unpaired alignments should be converted to a single
// discordant paired-end alignment.
assert_eq(0, ndiscord_);
if(nconcord_ == 0 && nunpair1_ == 1 && nunpair2_ == 1) {
convertUnpairedToDiscordant();
}
doneDiscord_ = true;
exitDiscord_ =
((ndiscord_ > 0) ?
ReportingState::EXIT_WITH_ALIGNMENTS :
ReportingState::EXIT_NO_ALIGNMENTS);
}
assert(!paired_ || exitDiscord_ > ReportingState::EXIT_DID_NOT_EXIT);
doneUnpair_ = done_ = true;
assert(done());
}
/**
* Populate given counters with the number of various kinds of alignments
* to report for this read. Concordant alignments are preferable to (and
* mutually exclusive with) discordant alignments, and paired-end
* alignments are preferable to unpaired alignments.
*
* The caller also needs some additional information for the case where a
* pair or unpaired read aligns repetitively. If the read is paired-end
* and the paired-end has repetitive concordant alignments, that should be
* reported, and 'pairMax' is set to true to indicate this. If the read is
* paired-end, does not have any conordant alignments, but does have
* repetitive alignments for one or both mates, then that should be
* reported, and 'unpair1Max' and 'unpair2Max' are set accordingly.
*
* Note that it's possible in the case of a paired-end read for the read to
* have repetitive concordant alignments, but for one mate to have a unique
* unpaired alignment.
*/
void ReportingState::getReport(
uint64_t& nconcordAln, // # concordant alignments to report
uint64_t& ndiscordAln, // # discordant alignments to report
uint64_t& nunpair1Aln, // # unpaired alignments for mate #1 to report
uint64_t& nunpair2Aln, // # unpaired alignments for mate #2 to report
uint64_t& nunpairRepeat1Aln, // # unpaired alignments for mate #1 to report
uint64_t& nunpairRepeat2Aln, // # unpaired alignments for mate #2 to report
bool& pairMax, // repetitive concordant alignments
bool& unpair1Max, // repetitive alignments for mate #1
bool& unpair2Max) // repetitive alignments for mate #2
const
{
nconcordAln = ndiscordAln = nunpair1Aln = nunpair2Aln = 0;
nunpairRepeat1Aln = nunpairRepeat2Aln = 0;
pairMax = unpair1Max = unpair2Max = false;
assert_gt(p_.khits, 0);
assert_gt(p_.mhits, 0);
if(paired_) {
// Do we have 1 or more concordant alignments to report?
if(exitConcord_ == ReportingState::EXIT_SHORT_CIRCUIT_k) {
// k at random
assert_geq(nconcord_, (uint64_t)p_.khits);
nconcordAln = p_.khits;
return;
} else if(exitConcord_ == ReportingState::EXIT_SHORT_CIRCUIT_M) {
assert(p_.msample);
assert_gt(nconcord_, 0);
pairMax = true; // repetitive concordant alignments
if(p_.mixed) {
unpair1Max = nunpair1_ > (uint64_t)p_.mhits;
unpair2Max = nunpair2_ > (uint64_t)p_.mhits;
}
// Not sure if this is OK
nconcordAln = 1; // 1 at random
return;
} else if(exitConcord_ == ReportingState::EXIT_WITH_ALIGNMENTS) {
assert_gt(nconcord_, 0);
// <= k at random
nconcordAln = min<uint64_t>(p_.khits, nconcord_);
}
assert(!p_.mhitsSet() || nconcord_ <= (uint64_t)p_.mhits+1);
// Do we have a discordant alignment to report?
if(exitDiscord_ == ReportingState::EXIT_WITH_ALIGNMENTS) {
// Report discordant
assert(p_.discord);
ndiscordAln = 1;
return;
}
}
assert_neq(ReportingState::EXIT_SHORT_CIRCUIT_TRUMPED, exitUnpair1_);
assert_neq(ReportingState::EXIT_SHORT_CIRCUIT_TRUMPED, exitUnpair2_);
if((paired_ && !p_.mixed) || nunpair1_ + nunpair2_ == 0) {
// Unpaired alignments either not reportable or non-existant
return;
}
// Do we have 1 or more alignments for mate #1 to report?
if(exitUnpair1_ == ReportingState::EXIT_SHORT_CIRCUIT_k) {
// k at random
assert_geq(nunpair1_, (uint64_t)p_.khits);
nunpair1Aln = p_.khits;
} else if(exitUnpair1_ == ReportingState::EXIT_SHORT_CIRCUIT_M) {
assert(p_.msample);
assert_gt(nunpair1_, 0);
unpair1Max = true; // repetitive alignments for mate #1
nunpair1Aln = 1; // 1 at random
} else if(exitUnpair1_ == ReportingState::EXIT_WITH_ALIGNMENTS) {
assert_gt(nunpair1_, 0);
// <= k at random
nunpair1Aln = min<uint64_t>(nunpair1_, (uint64_t)p_.khits);
}
assert(!p_.mhitsSet() || paired_ || nunpair1_ <= (uint64_t)p_.mhits+1);
if(p_.repeat) nunpairRepeat1Aln = nunpairRepeat1_;
// Do we have 2 or more alignments for mate #2 to report?
if(exitUnpair2_ == ReportingState::EXIT_SHORT_CIRCUIT_k) {
// k at random
nunpair2Aln = p_.khits;
} else if(exitUnpair2_ == ReportingState::EXIT_SHORT_CIRCUIT_M) {
assert(p_.msample);
assert_gt(nunpair2_, 0);
unpair2Max = true; // repetitive alignments for mate #1
nunpair2Aln = 1; // 1 at random
} else if(exitUnpair2_ == ReportingState::EXIT_WITH_ALIGNMENTS) {
assert_gt(nunpair2_, 0);
// <= k at random
nunpair2Aln = min<uint64_t>(nunpair2_, (uint64_t)p_.khits);
}
assert(!p_.mhitsSet() || paired_ || nunpair2_ <= (uint64_t)p_.mhits+1);
if(p_.repeat) nunpairRepeat2Aln = nunpairRepeat2_;
}
/**
* Given the number of alignments in a category, check whether we
* short-circuited out of the category. Set the done and exit arguments to
* indicate whether and how we short-circuited.
*/
inline void ReportingState::areDone(
uint64_t cnt, // # alignments in category
bool& done, // out: whether we short-circuited out of category
int& exit) const // out: if done, how we short-circuited (-k? -m? etc)
{
assert(!done);
// Have we exceeded the -k limit?
assert_gt(p_.khits, 0);
assert_gt(p_.mhits, 0);
if(cnt >= (uint64_t)p_.khits && !p_.mhitsSet()) {
done = true;
exit = ReportingState::EXIT_SHORT_CIRCUIT_k;
}
// Have we exceeded the -m or -M limit?
else if(p_.mhitsSet() && cnt > (uint64_t)p_.mhits) {
done = true;
assert(p_.msample);
exit = ReportingState::EXIT_SHORT_CIRCUIT_M;
}
}
#ifdef ALN_SINK_MAIN
#include <iostream>
bool testDones(
const ReportingState& st,
bool done1,
bool done2,
bool done3,
bool done4,
bool done5,
bool done6)
{
assert(st.doneConcordant() == done1);
assert(st.doneDiscordant() == done2);
assert(st.doneUnpaired(true) == done3);
assert(st.doneUnpaired(false) == done4);
assert(st.doneUnpaired() == done5);
assert(st.done() == done6);
assert(st.repOk());
return true;
}
int main(void) {
cerr << "Case 1 (simple unpaired 1) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
2, // khits
0, // mhits
0, // pengap
false, // msample
false, // discord
false); // mixed
ReportingState st(rp);
st.nextRead(false); // unpaired read
assert(testDones(st, true, true, false, true, false, false));
st.foundUnpaired(true);
assert(testDones(st, true, true, false, true, false, false));
st.foundUnpaired(true);
assert(testDones(st, true, true, true, true, true, true));
st.finish();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(0, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(2, st.numUnpaired1());
assert_eq(0, st.numUnpaired2());
assert(st.repOk());
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(0, nconcord);
assert_eq(0, ndiscord);
assert_eq(2, nunpair1);
assert_eq(0, nunpair2);
assert(!pairMax);
assert(!unpair1Max);
assert(!unpair2Max);
}
cerr << "PASSED" << endl;
cerr << "Case 2 (simple unpaired 1) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
2, // khits
3, // mhits
0, // pengap
false, // msample
false, // discord
false); // mixed
ReportingState st(rp);
st.nextRead(false); // unpaired read
assert(testDones(st, true, true, false, true, false, false));
st.foundUnpaired(true);
assert(testDones(st, true, true, false, true, false, false));
st.foundUnpaired(true);
assert(testDones(st, true, true, false, true, false, false));
st.foundUnpaired(true);
assert(testDones(st, true, true, false, true, false, false));
st.foundUnpaired(true);
assert(testDones(st, true, true, true, true, true, true));
assert_eq(0, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(4, st.numUnpaired1());
assert_eq(0, st.numUnpaired2());
st.finish();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(0, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(4, st.numUnpaired1());
assert_eq(0, st.numUnpaired2());
assert(st.repOk());
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(0, nconcord);
assert_eq(0, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(!pairMax);
assert(unpair1Max);
assert(!unpair2Max);
}
cerr << "PASSED" << endl;
cerr << "Case 3 (simple paired 1) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
2, // khits
3, // mhits
0, // pengap
false, // msample
false, // discord
false); // mixed
ReportingState st(rp);
st.nextRead(true); // unpaired read
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, true, true, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(4, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(4, st.numUnpaired1());
assert_eq(4, st.numUnpaired2());
st.finish();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(4, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(4, st.numUnpaired1());
assert_eq(4, st.numUnpaired2());
assert(st.repOk());
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(0, nconcord);
assert_eq(0, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(pairMax);
assert(!unpair1Max); // because !mixed
assert(!unpair2Max); // because !mixed
}
cerr << "PASSED" << endl;
cerr << "Case 4 (simple paired 2) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
2, // khits
3, // mhits
0, // pengap
false, // msample
true, // discord
true); // mixed
ReportingState st(rp);
st.nextRead(true); // unpaired read
assert(testDones(st, false, false, false, false, false, false));
st.foundUnpaired(true);
assert(testDones(st, false, false, false, false, false, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, false, false, false, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, false, false, false, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, true, false, false, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, false, false, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, false, false, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, false, false, false));
st.foundUnpaired(false);
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, false, true, true, true, true, false));
st.foundConcordant();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(4, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(4, st.numUnpaired1());
assert_eq(4, st.numUnpaired2());
st.finish();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(4, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(4, st.numUnpaired1());
assert_eq(4, st.numUnpaired2());
assert(st.repOk());
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(0, nconcord);
assert_eq(0, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(pairMax);
assert(unpair1Max);
assert(unpair2Max);
}
cerr << "PASSED" << endl;
cerr << "Case 5 (potential discordant after concordant) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
2, // khits
3, // mhits
0, // pengap
false, // msample
true, // discord
true); // mixed
ReportingState st(rp);
st.nextRead(true);
assert(testDones(st, false, false, false, false, false, false));
st.foundUnpaired(true);
st.foundUnpaired(false);
st.foundConcordant();
assert(testDones(st, false, true, false, false, false, false));
st.finish();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(1, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(1, st.numUnpaired1());
assert_eq(1, st.numUnpaired2());
assert(st.repOk());
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(1, nconcord);
assert_eq(0, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(!pairMax);
assert(!unpair1Max);
assert(!unpair2Max);
}
cerr << "PASSED" << endl;
cerr << "Case 6 (true discordant) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
2, // khits
3, // mhits
0, // pengap
false, // msample
true, // discord
true); // mixed
ReportingState st(rp);
st.nextRead(true);
assert(testDones(st, false, false, false, false, false, false));
st.foundUnpaired(true);
st.foundUnpaired(false);
assert(testDones(st, false, false, false, false, false, false));
st.finish();
assert(testDones(st, true, true, true, true, true, true));
assert_eq(0, st.numConcordant());
assert_eq(1, st.numDiscordant());
assert_eq(0, st.numUnpaired1());
assert_eq(0, st.numUnpaired2());
assert(st.repOk());
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(0, nconcord);
assert_eq(1, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(!pairMax);
assert(!unpair1Max);
assert(!unpair2Max);
}
cerr << "PASSED" << endl;
cerr << "Case 7 (unaligned pair & uniquely aligned mate, mixed-mode) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
1, // khits
1, // mhits
0, // pengap
false, // msample
true, // discord
true); // mixed
ReportingState st(rp);
st.nextRead(true); // unpaired read
// assert(st.doneConcordant() == done1);
// assert(st.doneDiscordant() == done2);
// assert(st.doneUnpaired(true) == done3);
// assert(st.doneUnpaired(false) == done4);
// assert(st.doneUnpaired() == done5);
// assert(st.done() == done6);
st.foundUnpaired(true);
assert(testDones(st, false, false, false, false, false, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, true, false, false, false));
assert_eq(0, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(2, st.numUnpaired1());
assert_eq(0, st.numUnpaired2());
st.finish();
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(0, nconcord);
assert_eq(0, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(!pairMax);
assert(unpair1Max);
assert(!unpair2Max);
}
cerr << "PASSED" << endl;
cerr << "Case 8 (unaligned pair & uniquely aligned mate, NOT mixed-mode) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
1, // khits
1, // mhits
0, // pengap
false, // msample
true, // discord
false); // mixed
ReportingState st(rp);
st.nextRead(true); // unpaired read
// assert(st.doneConcordant() == done1);
// assert(st.doneDiscordant() == done2);
// assert(st.doneUnpaired(true) == done3);
// assert(st.doneUnpaired(false) == done4);
// assert(st.doneUnpaired() == done5);
// assert(st.done() == done6);
st.foundUnpaired(true);
assert(testDones(st, false, false, true, true, true, false));
st.foundUnpaired(true);
assert(testDones(st, false, true, true, true, true, false));
assert_eq(0, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(2, st.numUnpaired1());
assert_eq(0, st.numUnpaired2());
st.finish();
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(0, nconcord);
assert_eq(0, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(!pairMax);
assert(!unpair1Max); // not really relevant
assert(!unpair2Max); // not really relevant
}
cerr << "PASSED" << endl;
cerr << "Case 9 (repetitive pair, only one mate repetitive) ... ";
{
uint64_t nconcord = 0, ndiscord = 0, nunpair1 = 0, nunpair2 = 0;
bool pairMax = false, unpair1Max = false, unpair2Max = false;
ReportingParams rp(
1, // khits
1, // mhits
0, // pengap
true, // msample
true, // discord
true); // mixed
ReportingState st(rp);
st.nextRead(true); // unpaired read
// assert(st.doneConcordant() == done1);
// assert(st.doneDiscordant() == done2);
// assert(st.doneUnpaired(true) == done3);
// assert(st.doneUnpaired(false) == done4);
// assert(st.doneUnpaired() == done5);
// assert(st.done() == done6);
st.foundConcordant();
assert(st.repOk());
st.foundUnpaired(true);
assert(st.repOk());
st.foundUnpaired(false);
assert(st.repOk());
assert(testDones(st, false, true, false, false, false, false));
assert(st.repOk());
st.foundConcordant();
assert(st.repOk());
st.foundUnpaired(true);
assert(st.repOk());
assert(testDones(st, true, true, true, false, false, false));
assert_eq(2, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(2, st.numUnpaired1());
assert_eq(1, st.numUnpaired2());
st.foundUnpaired(false);
assert(st.repOk());
assert(testDones(st, true, true, true, true, true, true));
assert_eq(2, st.numConcordant());
assert_eq(0, st.numDiscordant());
assert_eq(2, st.numUnpaired1());
assert_eq(2, st.numUnpaired2());
st.finish();
st.getReport(nconcord, ndiscord, nunpair1, nunpair2,
pairMax, unpair1Max, unpair2Max);
assert_eq(1, nconcord);
assert_eq(0, ndiscord);
assert_eq(0, nunpair1);
assert_eq(0, nunpair2);
assert(pairMax);
assert(unpair1Max); // not really relevant
assert(unpair2Max); // not really relevant
}
cerr << "PASSED" << endl;
}
#endif /*def ALN_SINK_MAIN*/
|