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#!/bin/sh
#
# Downloads sequence for the GRCm38 release 81 version of M. Musculus (mouse) from
# Ensembl.
#
# By default, this script builds and index for just the base files,
# since alignments to those sequences are the most useful. To change
# which categories are built by this script, edit the CHRS_TO_INDEX
# variable below.
#
ENSEMBL_RELEASE=84
ENSEMBL_GRCm38_BASE=ftp://ftp.ensembl.org/pub/release-${ENSEMBL_RELEASE}/fasta/mus_musculus/dna
get() {
file=$1
if ! wget --version >/dev/null 2>/dev/null ; then
if ! curl --version >/dev/null 2>/dev/null ; then
echo "Please install wget or curl somewhere in your PATH"
exit 1
fi
curl -o `basename $1` $1
return $?
else
wget $1
return $?
fi
}
HISAT2_BUILD_EXE=./hisat2-build
if [ ! -x "$HISAT2_BUILD_EXE" ] ; then
if ! which hisat2-build ; then
echo "Could not find hisat2-build in current directory or in PATH"
exit 1
else
HISAT2_BUILD_EXE=`which hisat2-build`
fi
fi
rm -f genome.fa
F=Mus_musculus.GRCm38.dna.primary_assembly.fa
if [ ! -f $F ] ; then
get ${ENSEMBL_GRCm38_BASE}/$F.gz || (echo "Error getting $F" && exit 1)
gunzip $F.gz || (echo "Error unzipping $F" && exit 1)
mv $F genome.fa
fi
CMD="${HISAT2_BUILD_EXE} genome.fa genome"
echo Running $CMD
if $CMD ; then
echo "genome index built; you may remove fasta files"
else
echo "Index building failed; see error message"
fi
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