1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443 444 445 446 447 448 449 450 451 452 453 454 455 456 457 458 459 460 461 462 463 464 465 466 467 468 469 470 471 472 473
|
/* faidx.c -- FASTA random access.
Copyright (C) 2008, 2009, 2013, 2014 Genome Research Ltd.
Portions copyright (C) 2011 Broad Institute.
Author: Heng Li <lh3@sanger.ac.uk>
Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:
The above copyright notice and this permission notice shall be included in
all copies or substantial portions of the Software.
THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL
THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER
DEALINGS IN THE SOFTWARE. */
#include "config.h"
#include <ctype.h>
#include <string.h>
#include <stdlib.h>
#include <stdio.h>
#include <stdint.h>
#include "htslib/bgzf.h"
#include "htslib/faidx.h"
#include "htslib/khash.h"
#ifdef _USE_KNETFILE
#include "htslib/knetfile.h"
#endif
typedef struct {
int32_t line_len, line_blen;
int64_t len;
uint64_t offset;
} faidx1_t;
KHASH_MAP_INIT_STR(s, faidx1_t)
struct __faidx_t {
BGZF *bgzf;
int n, m;
char **name;
khash_t(s) *hash;
};
#ifndef kroundup32
#define kroundup32(x) (--(x), (x)|=(x)>>1, (x)|=(x)>>2, (x)|=(x)>>4, (x)|=(x)>>8, (x)|=(x)>>16, ++(x))
#endif
static inline void fai_insert_index(faidx_t *idx, const char *name, int len, int line_len, int line_blen, uint64_t offset)
{
khint_t k;
int ret;
faidx1_t t;
if (idx->n == idx->m) {
idx->m = idx->m? idx->m<<1 : 16;
idx->name = (char**)realloc(idx->name, sizeof(char*) * idx->m);
}
idx->name[idx->n] = strdup(name);
k = kh_put(s, idx->hash, idx->name[idx->n], &ret);
t.len = len; t.line_len = line_len; t.line_blen = line_blen; t.offset = offset;
kh_value(idx->hash, k) = t;
++idx->n;
}
faidx_t *fai_build_core(BGZF *bgzf)
{
char c, *name;
int l_name, m_name;
int line_len, line_blen, state;
int l1, l2;
faidx_t *idx;
uint64_t offset;
int64_t len;
idx = (faidx_t*)calloc(1, sizeof(faidx_t));
idx->hash = kh_init(s);
name = 0; l_name = m_name = 0;
len = line_len = line_blen = -1; state = 0; l1 = l2 = -1; offset = 0;
while ( (c=bgzf_getc(bgzf))>=0 ) {
if (c == '\n') { // an empty line
if (state == 1) {
offset = bgzf_utell(bgzf);
continue;
} else if ((state == 0 && len < 0) || state == 2) continue;
}
if (c == '>') { // fasta header
if (len >= 0)
fai_insert_index(idx, name, len, line_len, line_blen, offset);
l_name = 0;
while ( (c=bgzf_getc(bgzf))>=0 && !isspace(c)) {
if (m_name < l_name + 2) {
m_name = l_name + 2;
kroundup32(m_name);
name = (char*)realloc(name, m_name);
}
name[l_name++] = c;
}
name[l_name] = '\0';
if ( c<0 ) {
fprintf(stderr, "[fai_build_core] the last entry has no sequence\n");
free(name); fai_destroy(idx);
return 0;
}
if (c != '\n') while ( (c=bgzf_getc(bgzf))>=0 && c != '\n');
state = 1; len = 0;
offset = bgzf_utell(bgzf);
} else {
if (state == 3) {
fprintf(stderr, "[fai_build_core] inlined empty line is not allowed in sequence '%s'.\n", name);
free(name); fai_destroy(idx);
return 0;
}
if (state == 2) state = 3;
l1 = l2 = 0;
do {
++l1;
if (isgraph(c)) ++l2;
} while ( (c=bgzf_getc(bgzf))>=0 && c != '\n');
if (state == 3 && l2) {
fprintf(stderr, "[fai_build_core] different line length in sequence '%s'.\n", name);
free(name); fai_destroy(idx);
return 0;
}
++l1; len += l2;
if (state == 1) line_len = l1, line_blen = l2, state = 0;
else if (state == 0) {
if (l1 != line_len || l2 != line_blen) state = 2;
}
}
}
if ( name )
fai_insert_index(idx, name, len, line_len, line_blen, offset);
else
{
free(idx);
return NULL;
}
free(name);
return idx;
}
void fai_save(const faidx_t *fai, FILE *fp)
{
khint_t k;
int i;
for (i = 0; i < fai->n; ++i) {
faidx1_t x;
k = kh_get(s, fai->hash, fai->name[i]);
x = kh_value(fai->hash, k);
#ifdef _WIN32
fprintf(fp, "%s\t%d\t%ld\t%d\t%d\n", fai->name[i], (int)x.len, (long)x.offset, (int)x.line_blen, (int)x.line_len);
#else
fprintf(fp, "%s\t%d\t%lld\t%d\t%d\n", fai->name[i], (int)x.len, (long long)x.offset, (int)x.line_blen, (int)x.line_len);
#endif
}
}
faidx_t *fai_read(FILE *fp)
{
faidx_t *fai;
char *buf, *p;
int len, line_len, line_blen;
#ifdef _WIN32
long offset;
#else
long long offset;
#endif
fai = (faidx_t*)calloc(1, sizeof(faidx_t));
fai->hash = kh_init(s);
buf = (char*)calloc(0x10000, 1);
while (!feof(fp) && fgets(buf, 0x10000, fp)) {
for (p = buf; *p && isgraph(*p); ++p);
*p = 0; ++p;
#ifdef _WIN32
sscanf(p, "%d%ld%d%d", &len, &offset, &line_blen, &line_len);
#else
sscanf(p, "%d%lld%d%d", &len, &offset, &line_blen, &line_len);
#endif
fai_insert_index(fai, buf, len, line_len, line_blen, offset);
}
free(buf);
return fai;
}
void fai_destroy(faidx_t *fai)
{
int i;
for (i = 0; i < fai->n; ++i) free(fai->name[i]);
free(fai->name);
kh_destroy(s, fai->hash);
if (fai->bgzf) bgzf_close(fai->bgzf);
free(fai);
}
int fai_build(const char *fn)
{
char *str;
BGZF *bgzf;
FILE *fp;
faidx_t *fai;
str = (char*)calloc(strlen(fn) + 5, 1);
sprintf(str, "%s.fai", fn);
bgzf = bgzf_open(fn, "r");
if ( !bgzf ) {
fprintf(stderr, "[fai_build] fail to open the FASTA file %s\n",fn);
free(str);
return -1;
}
if ( bgzf->is_compressed ) bgzf_index_build_init(bgzf);
fai = fai_build_core(bgzf);
if ( !fai )
{
if ( bgzf->is_compressed && bgzf->is_gzip ) fprintf(stderr,"Cannot index files compressed with gzip, please use bgzip\n");
return -1;
}
if ( bgzf->is_compressed ) bgzf_index_dump(bgzf, fn, ".gzi");
bgzf_close(bgzf);
fp = fopen(str, "wb");
if ( !fp ) {
fprintf(stderr, "[fai_build] fail to write FASTA index %s\n",str);
fai_destroy(fai); free(str);
return -1;
}
fai_save(fai, fp);
fclose(fp);
free(str);
fai_destroy(fai);
return 0;
}
#ifdef _USE_KNETFILE
FILE *download_and_open(const char *fn)
{
const int buf_size = 1 * 1024 * 1024;
uint8_t *buf;
FILE *fp;
knetFile *fp_remote;
const char *url = fn;
const char *p;
int l = strlen(fn);
for (p = fn + l - 1; p >= fn; --p)
if (*p == '/') break;
fn = p + 1;
// First try to open a local copy
fp = fopen(fn, "r");
if (fp)
return fp;
// If failed, download from remote and open
fp_remote = knet_open(url, "rb");
if (fp_remote == 0) {
fprintf(stderr, "[download_from_remote] fail to open remote file %s\n",url);
return NULL;
}
if ((fp = fopen(fn, "wb")) == 0) {
fprintf(stderr, "[download_from_remote] fail to create file in the working directory %s\n",fn);
knet_close(fp_remote);
return NULL;
}
buf = (uint8_t*)calloc(buf_size, 1);
while ((l = knet_read(fp_remote, buf, buf_size)) != 0)
fwrite(buf, 1, l, fp);
free(buf);
fclose(fp);
knet_close(fp_remote);
return fopen(fn, "r");
}
#endif
faidx_t *fai_load(const char *fn)
{
char *str;
FILE *fp;
faidx_t *fai;
str = (char*)calloc(strlen(fn) + 5, 1);
sprintf(str, "%s.fai", fn);
#ifdef _USE_KNETFILE
if (strstr(fn, "ftp://") == fn || strstr(fn, "http://") == fn)
{
fp = download_and_open(str);
if ( !fp )
{
fprintf(stderr, "[fai_load] failed to open remote FASTA index %s\n", str);
free(str);
return 0;
}
}
else
#endif
fp = fopen(str, "rb");
if (fp == 0) {
fprintf(stderr, "[fai_load] build FASTA index.\n");
fai_build(fn);
fp = fopen(str, "rb");
if (fp == 0) {
fprintf(stderr, "[fai_load] fail to open FASTA index.\n");
free(str);
return 0;
}
}
fai = fai_read(fp);
fclose(fp);
fai->bgzf = bgzf_open(fn, "rb");
free(str);
if (fai->bgzf == 0) {
fprintf(stderr, "[fai_load] fail to open FASTA file.\n");
return 0;
}
if ( fai->bgzf->is_compressed==1 )
{
if ( bgzf_index_load(fai->bgzf, fn, ".gzi") < 0 )
{
fprintf(stderr, "[fai_load] failed to load .gzi index: %s[.gzi]\n", fn);
fai_destroy(fai);
return NULL;
}
}
return fai;
}
char *fai_fetch(const faidx_t *fai, const char *str, int *len)
{
char *s, c;
int i, l, k, name_end;
khiter_t iter;
faidx1_t val;
khash_t(s) *h;
int beg, end;
beg = end = -1;
h = fai->hash;
name_end = l = strlen(str);
s = (char*)malloc(l+1);
// remove space
for (i = k = 0; i < l; ++i)
if (!isspace(str[i])) s[k++] = str[i];
s[k] = 0; l = k;
// determine the sequence name
for (i = l - 1; i >= 0; --i) if (s[i] == ':') break; // look for colon from the end
if (i >= 0) name_end = i;
if (name_end < l) { // check if this is really the end
int n_hyphen = 0;
for (i = name_end + 1; i < l; ++i) {
if (s[i] == '-') ++n_hyphen;
else if (!isdigit(s[i]) && s[i] != ',') break;
}
if (i < l || n_hyphen > 1) name_end = l; // malformated region string; then take str as the name
s[name_end] = 0;
iter = kh_get(s, h, s);
if (iter == kh_end(h)) { // cannot find the sequence name
iter = kh_get(s, h, str); // try str as the name
if (iter == kh_end(h)) {
*len = 0;
free(s); return 0;
} else s[name_end] = ':', name_end = l;
}
} else iter = kh_get(s, h, str);
if(iter == kh_end(h)) {
fprintf(stderr, "[fai_fetch] Warning - Reference %s not found in FASTA file, returning empty sequence\n", str);
free(s);
*len = -2;
return 0;
};
val = kh_value(h, iter);
// parse the interval
if (name_end < l) {
for (i = k = name_end + 1; i < l; ++i)
if (s[i] != ',') s[k++] = s[i];
s[k] = 0;
beg = atoi(s + name_end + 1);
for (i = name_end + 1; i != k; ++i) if (s[i] == '-') break;
end = i < k? atoi(s + i + 1) : val.len;
if (beg > 0) --beg;
} else beg = 0, end = val.len;
if (beg >= val.len) beg = val.len;
if (end >= val.len) end = val.len;
if (beg > end) beg = end;
free(s);
// now retrieve the sequence
int ret = bgzf_useek(fai->bgzf, val.offset + beg / val.line_blen * val.line_len + beg % val.line_blen, SEEK_SET);
if ( ret<0 )
{
*len = -1;
fprintf(stderr, "[fai_fetch] Error: fai_fetch failed. (Seeking in a compressed, .gzi unindexed, file?)\n");
return NULL;
}
l = 0;
s = (char*)malloc(end - beg + 2);
while ( (c=bgzf_getc(fai->bgzf))>=0 && l < end - beg )
if (isgraph(c)) s[l++] = c;
s[l] = '\0';
*len = l;
return s;
}
int faidx_nseq(const faidx_t *fai)
{
return fai->n;
}
const char *faidx_iseq(const faidx_t *fai, int i)
{
return fai->name[i];
}
int faidx_seq_len(const faidx_t *fai, const char *seq)
{
khint_t k = kh_get(s, fai->hash, seq);
if ( k == kh_end(fai->hash) ) return -1;
return kh_val(fai->hash, k).len;
}
char *faidx_fetch_seq(const faidx_t *fai, const char *c_name, int p_beg_i, int p_end_i, int *len)
{
int l;
char c;
khiter_t iter;
faidx1_t val;
char *seq=NULL;
// Adjust position
iter = kh_get(s, fai->hash, c_name);
if (iter == kh_end(fai->hash))
{
*len = -2;
fprintf(stderr, "[fai_fetch_seq] The sequence \"%s\" not found\n", c_name);
return NULL;
}
val = kh_value(fai->hash, iter);
if(p_end_i < p_beg_i) p_beg_i = p_end_i;
if(p_beg_i < 0) p_beg_i = 0;
else if(val.len <= p_beg_i) p_beg_i = val.len - 1;
if(p_end_i < 0) p_end_i = 0;
else if(val.len <= p_end_i) p_end_i = val.len - 1;
// Now retrieve the sequence
int ret = bgzf_useek(fai->bgzf, val.offset + p_beg_i / val.line_blen * val.line_len + p_beg_i % val.line_blen, SEEK_SET);
if ( ret<0 )
{
*len = -1;
fprintf(stderr, "[fai_fetch_seq] Error: fai_fetch failed. (Seeking in a compressed, .gzi unindexed, file?)\n");
return NULL;
}
l = 0;
seq = (char*)malloc(p_end_i - p_beg_i + 2);
while ( (c=bgzf_getc(fai->bgzf))>=0 && l < p_end_i - p_beg_i + 1)
if (isgraph(c)) seq[l++] = c;
seq[l] = '\0';
*len = l;
return seq;
}
int faidx_has_seq(const faidx_t *fai, const char *seq)
{
khiter_t iter = kh_get(s, fai->hash, seq);
if (iter == kh_end(fai->hash)) return 0;
return 1;
}
|