1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284
|
package ij.plugin;
import ij.*;
import ij.gui.*;
import ij.process.*;
import ij.measure.*;
import ij.plugin.frame.Recorder;
import ij.plugin.filter.PlugInFilter;
import java.awt.*;
/** This plugin implements the Process/Binary/Make Binary
and Convert to Mask commands. */
public class Thresholder implements PlugIn, Measurements {
private double minThreshold;
private double maxThreshold;
boolean autoThreshold;
boolean skipDialog;
static boolean fill1 = true;
static boolean fill2 = true;
static boolean useBW = true;
static boolean useLocal = true;
boolean convertToMask;
public void run(String arg) {
convertToMask = arg.equals("mask");
skipDialog = arg.equals("skip") || convertToMask;
ImagePlus imp = WindowManager.getCurrentImage();
if (imp==null)
{IJ.noImage(); return;}
if (imp.getStackSize()==1) {
Undo.setup(Undo.COMPOUND_FILTER, imp);
applyThreshold(imp);
Undo.setup(Undo.COMPOUND_FILTER_DONE, imp);
} else
convertStack(imp);
}
void convertStack(ImagePlus imp) {
if (!(imp.getProcessor().getMinThreshold()==ImageProcessor.NO_THRESHOLD))
useLocal = false;
GenericDialog gd = new GenericDialog("Convert to Mask");
gd.addMessage("Convert all images in stack to binary?");
gd.addCheckbox("Calculate Threshold for Each Image", useLocal);
gd.addCheckbox("Black Background", Prefs.blackBackground);
gd.showDialog();
if (gd.wasCanceled()) return;
useLocal = gd.getNextBoolean();
Prefs.blackBackground = gd.getNextBoolean();
Undo.reset();
if (useLocal)
convertStackToBinary(imp);
else
applyThreshold(imp);
}
void applyThreshold(ImagePlus imp) {
imp.killRoi();
ImageProcessor ip = imp.getProcessor();
ip.resetBinaryThreshold();
int type = imp.getType();
if (type==ImagePlus.GRAY16 || type==ImagePlus.GRAY32) {
applyShortOrFloatThreshold(imp);
return;
}
if (!imp.lock()) return;
double saveMinThreshold = ip.getMinThreshold();
double saveMaxThreshold = ip.getMaxThreshold();
autoThreshold = saveMinThreshold==ImageProcessor.NO_THRESHOLD;
boolean useBlackAndWhite = true;
boolean noArgMacro =IJ.macroRunning() && Macro.getOptions()==null;
if (skipDialog)
fill1 = fill2 = useBlackAndWhite = true;
else if (!(autoThreshold||noArgMacro)) {
GenericDialog gd = new GenericDialog("Make Binary");
gd.addCheckbox("Thresholded pixels to foreground color", fill1);
gd.addCheckbox("Remaining pixels to background color", fill2);
gd.addMessage("");
gd.addCheckbox("Black foreground, white background", useBW);
gd.showDialog();
if (gd.wasCanceled())
{imp.unlock(); return;}
fill1 = gd.getNextBoolean();
fill2 = gd.getNextBoolean();
useBW = useBlackAndWhite = gd.getNextBoolean();
} else {
fill1 = fill2 = true;
convertToMask = true;
}
if (type!=ImagePlus.GRAY8)
convertToByte(imp);
ip = imp.getProcessor();
if (autoThreshold)
autoThreshold(ip);
else {
if (Recorder.record && !Recorder.scriptMode() && (!IJ.isMacro()||Recorder.recordInMacros))
Recorder.record("setThreshold", (int)saveMinThreshold, (int)saveMaxThreshold);
minThreshold = saveMinThreshold;
maxThreshold = saveMaxThreshold;
}
if (convertToMask && ip.isColorLut())
ip.setColorModel(ip.getDefaultColorModel());
int fcolor, bcolor;
ip.resetThreshold();
int savePixel = ip.getPixel(0,0);
if (useBlackAndWhite)
ip.setColor(Color.black);
else
ip.setColor(Toolbar.getForegroundColor());
ip.drawPixel(0,0);
fcolor = ip.getPixel(0,0);
if (useBlackAndWhite)
ip.setColor(Color.white);
else
ip.setColor(Toolbar.getBackgroundColor());
ip.drawPixel(0,0);
bcolor = ip.getPixel(0,0);
ip.setColor(Toolbar.getForegroundColor());
ip.putPixel(0,0,savePixel);
int[] lut = new int[256];
for (int i=0; i<256; i++) {
if (i>=minThreshold && i<=maxThreshold)
lut[i] = fill1?fcolor:(byte)i;
else {
lut[i] = fill2?bcolor:(byte)i;
}
}
if (imp.getStackSize()>1)
new StackProcessor(imp.getStack(), ip).applyTable(lut);
else
ip.applyTable(lut);
if (convertToMask) {
if (!imp.isInvertedLut()) {
setInvertedLut(imp);
fcolor = 255 - fcolor;
bcolor = 255 - bcolor;
}
if (Prefs.blackBackground)
ip.invertLut();
}
if (fill1 && fill2 && ((fcolor==0&&bcolor==255)||(fcolor==255&&bcolor==0)))
imp.getProcessor().setThreshold(fcolor, fcolor, ImageProcessor.NO_LUT_UPDATE);
imp.updateAndRepaintWindow();
imp.unlock();
}
void applyShortOrFloatThreshold(ImagePlus imp) {
if (!imp.lock()) return;
int width = imp.getWidth();
int height = imp.getHeight();
int size = width*height;
boolean isFloat = imp.getType()==ImagePlus.GRAY32;
int nSlices = imp.getStackSize();
ImageStack stack1 = imp.getStack();
ImageStack stack2 = new ImageStack(width, height);
ImageProcessor ip = imp.getProcessor();
float t1 = (float)ip.getMinThreshold();
float t2 = (float)ip.getMaxThreshold();
if (t1==ImageProcessor.NO_THRESHOLD) {
//ip.resetMinAndMax();
double min = ip.getMin();
double max = ip.getMax();
ip = ip.convertToByte(true);
autoThreshold(ip);
t1 = (float)(min + (max-min)*(minThreshold/255.0));
t2 = (float)(min + (max-min)*(maxThreshold/255.0));
}
float value;
ImageProcessor ip1, ip2;
IJ.showStatus("Converting to mask");
for(int i=1; i<=nSlices; i++) {
IJ.showProgress(i, nSlices);
String label = stack1.getSliceLabel(i);
ip1 = stack1.getProcessor(i);
ip2 = new ByteProcessor(width, height);
for (int j=0; j<size; j++) {
value = ip1.getf(j);
if (value>=t1 && value<=t2)
ip2.set(j, 255);
else
ip2.set(j, 0);
}
stack2.addSlice(label, ip2);
}
imp.setStack(null, stack2);
ImageStack stack = imp.getStack();
stack.setColorModel(LookUpTable.createGrayscaleColorModel(!Prefs.blackBackground));
imp.setStack(null, stack);
if (imp.isComposite()) {
CompositeImage ci = (CompositeImage)imp;
ci.setMode(CompositeImage.GRAYSCALE);
ci.resetDisplayRanges();
ci.updateAndDraw();
}
imp.getProcessor().setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE);
IJ.showStatus("");
imp.unlock();
}
void convertStackToBinary(ImagePlus imp) {
int nSlices = imp.getStackSize();
if ((imp.getBitDepth()!=8)) {
IJ.showStatus("Converting to byte");
ImageStack stack1 = imp.getStack();
ImageStack stack2 = new ImageStack(imp.getWidth(), imp.getHeight());
for(int i=1; i<=nSlices; i++) {
IJ.showProgress(i, nSlices);
String label = stack1.getSliceLabel(i);
ImageProcessor ip = stack1.getProcessor(i);
ip.resetMinAndMax();
stack2.addSlice(label, ip.convertToByte(true));
}
imp.setStack(null, stack2);
}
ImageStack stack = imp.getStack();
IJ.showStatus("Auto-thresholding");
for(int i=1; i<=nSlices; i++) {
IJ.showProgress(i, nSlices);
ImageProcessor ip = stack.getProcessor(i);
autoThreshold(ip);
int[] lut = new int[256];
for (int j=0; j<256; j++) {
if (j>=minThreshold && j<=maxThreshold)
lut[j] = (byte)255;
else
lut[j] = 0;
}
ip.applyTable(lut);
}
stack.setColorModel(LookUpTable.createGrayscaleColorModel(!Prefs.blackBackground));
imp.setStack(null, stack);
imp.getProcessor().setThreshold(255, 255, ImageProcessor.NO_LUT_UPDATE);
if (imp.isComposite()) {
CompositeImage ci = (CompositeImage)imp;
ci.setMode(CompositeImage.GRAYSCALE);
ci.resetDisplayRanges();
ci.updateAndDraw();
}
IJ.showStatus("");
}
void convertToByte(ImagePlus imp) {
ImageProcessor ip;
int currentSlice = imp.getCurrentSlice();
ImageStack stack1 = imp.getStack();
ImageStack stack2 = imp.createEmptyStack();
int nSlices = imp.getStackSize();
String label;
for(int i=1; i<=nSlices; i++) {
label = stack1.getSliceLabel(i);
ip = stack1.getProcessor(i);
ip.setMinAndMax(0, 255);
stack2.addSlice(label, ip.convertToByte(true));
}
imp.setStack(null, stack2);
imp.setSlice(currentSlice);
imp.setCalibration(imp.getCalibration()); //update calibration
}
void setInvertedLut(ImagePlus imp) {
ImageProcessor ip = imp.getProcessor();
ip.invertLut();
int nImages = imp.getStackSize();
if (nImages==1)
ip.invert();
else {
ImageStack stack = imp.getStack();
for (int slice=1; slice<=nImages; slice++)
stack.getProcessor(slice).invert();
stack.setColorModel(ip.getColorModel());
}
}
void autoThreshold(ImageProcessor ip) {
ip.setAutoThreshold(ImageProcessor.ISODATA2, ImageProcessor.NO_LUT_UPDATE);
minThreshold = ip.getMinThreshold();
maxThreshold = ip.getMaxThreshold();
}
}
|