1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261
|
package ij.plugin;
import ij.plugin.*;
import ij.*;
import ij.process.*;
import ij.gui.*;
import ij.measure.*;
import ij.plugin.filter.Analyzer;
import ij.util.Tools;
import java.awt.*;
/** Implements the Image/Stacks/Plot Z-axis Profile command,
which plots the selection mean gray value versus slice number.
*/
public class ZAxisProfiler implements PlugIn, Measurements, PlotMaker {
private static String[] choices = {"time", "z-axis"};
private static String choice = choices[0];
private boolean showingDialog;
private ImagePlus imp;
private boolean isPlotMaker;
private boolean timeProfile;
private boolean firstTime = true;
private String options;
/** Returns a Plot of the selection mean gray value versus slice number. */
public static Plot getPlot(ImagePlus imp) {
return getPlot(imp, "time");
}
/** Returns a Plot of the selection mean versus slice number for the
specified hyperstack, where 'options' can be "time" or "z-axis". */
public static Plot getPlot(ImagePlus imp, String options) {
ZAxisProfiler zap = new ZAxisProfiler();
zap.imp = imp;
zap.options = options;
zap.isPlotMaker = true;
Plot plot = zap.getPlot();
return plot;
}
public void run(String arg) {
imp = IJ.getImage();
if (imp.getStackSize()<2) {
IJ.error("ZAxisProfiler", "This command requires a stack.");
return;
}
isPlotMaker = true;
Plot plot = getPlot();
if (plot!=null) {
if (isPlotMaker)
plot.setPlotMaker(this);
plot.show();
}
}
public Plot getPlot() {
Roi roi = imp.getRoi();
ImageProcessor ip = imp.getProcessor();
double minThreshold = ip.getMinThreshold();
double maxThreshold = ip.getMaxThreshold();
float[] y;
boolean hyperstack = imp.isHyperStack();
if (hyperstack)
y = getHyperstackProfile(roi, minThreshold, maxThreshold);
else
y = getZAxisProfile(roi, minThreshold, maxThreshold);
if (y==null)
return null;
float[] x = new float[y.length];
String xAxisLabel = showingDialog&&choice.equals(choices[0])?"Frame":"Slice";
Calibration cal = imp.getCalibration();
if (cal.scaled()) {
double c = 1.0f;
double origin = 0;
if (timeProfile) {
c = (float) cal.frameInterval;
boolean zeroInterval = c==0;
if (zeroInterval)
c = 1;
String timeUnit = zeroInterval?"Frame":"["+cal.getTimeUnit()+"]";
xAxisLabel = timeUnit;
} else {
c = (float) cal.pixelDepth;
boolean zeroDepth = c==0;
if (zeroDepth)
c = 1;
origin = cal.zOrigin;
String depthUnit = zeroDepth?"Slice":"["+cal.getZUnit()+"]";
xAxisLabel = depthUnit;
}
for (int i=0; i<x.length; i++)
x[i] = (float)((i-cal.zOrigin)*c);
} else {
for (int i=0; i<x.length; i++)
x[i] = i+1;
}
String title;
if (roi!=null) {
Rectangle r = roi.getBounds();
title = imp.getTitle()+"-"+r.x+"-"+r.y;
} else
title = imp.getTitle()+"-0-0";
//String xAxisLabel = showingDialog&&choice.equals(choices[0])?"Frame":"Slice";
Plot plot = new Plot(title, xAxisLabel, "Mean", x, y);
if (x.length<=60) {
plot.setColor(Color.red);
plot.addPoints(x, y, Plot.CIRCLE);
plot.setColor(Color.black);
}
double ymin = ProfilePlot.getFixedMin();
double ymax= ProfilePlot.getFixedMax();
if (!(ymin==0.0 && ymax==0.0)) {
double[] a = Tools.getMinMax(x);
double xmin=a[0]; double xmax=a[1];
plot.setLimits(xmin, xmax, ymin, ymax);
}
if (!firstTime) {
int pos = imp.getCurrentSlice();
int size = imp.getStackSize();
if (hyperstack) {
if (timeProfile) {
pos = imp.getT();
size = imp.getNFrames();
} else {
pos = imp.getZ();
size = imp.getNSlices();
}
}
double xx = (pos-1.0)/(size-1.0);
if (xx==0.0)
plot.setLineWidth(2);
plot.setColor(Color.blue);
plot.drawNormalizedLine(xx, 0, xx, 1.0);
plot.setColor(Color.black);
plot.setLineWidth(1);
}
firstTime = false;
return plot;
}
public ImagePlus getSourceImage() {
return imp;
}
private float[] getHyperstackProfile(Roi roi, double minThreshold, double maxThreshold) {
int slices = imp.getNSlices();
int frames = imp.getNFrames();
int c = imp.getC();
int z = imp.getZ();
int t = imp.getT();
int size = slices;
if (firstTime)
timeProfile = slices==1 && frames>1;
if (options==null && slices>1 && frames>1 && (!isPlotMaker ||firstTime)) {
showingDialog = true;
GenericDialog gd = new GenericDialog("Profiler");
gd.addChoice("Profile", choices, choice);
gd.showDialog();
if (gd.wasCanceled())
return null;
choice = gd.getNextChoice();
timeProfile = choice.equals(choices[0]);
}
if (options!=null)
timeProfile = frames>1 && !options.contains("z");
if (timeProfile)
size = frames;
else
size = slices;
float[] values = new float[size];
Calibration cal = imp.getCalibration();
ResultsTable rt = new ResultsTable();
Analyzer analyzer = new Analyzer(imp, rt);
int measurements = Analyzer.getMeasurements();
boolean showResults = !isPlotMaker && measurements!=0 && measurements!=LIMIT;
measurements |= MEAN;
if (showResults) {
if (!Analyzer.resetCounter())
return null;
}
ImageStack stack = imp.getStack();
boolean showProgress = size>400 || stack.isVirtual();
for (int i=1; i<=size; i++) {
if (showProgress)
IJ.showProgress(i,size);
int index = 1;
if (timeProfile)
index = imp.getStackIndex(c, z, i);
else
index = imp.getStackIndex(c, i, t);
ImageProcessor ip = stack.getProcessor(index);
if (minThreshold!=ImageProcessor.NO_THRESHOLD)
ip.setThreshold(minThreshold,maxThreshold,ImageProcessor.NO_LUT_UPDATE);
ip.setRoi(roi);
ImageStatistics stats = ImageStatistics.getStatistics(ip, measurements, cal);
analyzer.saveResults(stats, roi);
values[i-1] = (float)stats.mean;
}
if (showResults)
rt.show("Results");
return values;
}
private float[] getZAxisProfile(Roi roi, double minThreshold, double maxThreshold) {
ImageStack stack = imp.getStack();
if (firstTime) {
int slices = imp.getNSlices();
int frames = imp.getNFrames();
timeProfile = slices==1 && frames>1;
}
int size = stack.getSize();
boolean showProgress = size>400 || stack.isVirtual();
float[] values = new float[size];
Calibration cal = imp.getCalibration();
ResultsTable rt = new ResultsTable();
Analyzer analyzer = new Analyzer(imp, rt);
int measurements = Analyzer.getMeasurements();
boolean showResults = !isPlotMaker && measurements!=0 && measurements!=LIMIT;
boolean showingLabels = firstTime && showResults && ((measurements&LABELS)!=0 || (measurements&SLICE)!=0);
measurements |= MEAN;
if (showResults) {
if (!Analyzer.resetCounter())
return null;
}
boolean isLine = roi!=null && roi.isLine();
int current = imp.getCurrentSlice();
for (int i=1; i<=size; i++) {
if (showProgress)
IJ.showProgress(i,size);
if (showingLabels)
imp.setSlice(i);
ImageProcessor ip = stack.getProcessor(i);
if (minThreshold!=ImageProcessor.NO_THRESHOLD)
ip.setThreshold(minThreshold,maxThreshold,ImageProcessor.NO_LUT_UPDATE);
ip.setRoi(roi);
ImageStatistics stats = null;
if (isLine)
stats = getLineStatistics(roi, ip, measurements, cal);
else
stats = ImageStatistics.getStatistics(ip, measurements, cal);
analyzer.saveResults(stats, roi);
values[i-1] = (float)stats.mean;
}
if (showResults)
rt.show("Results");
if (showingLabels)
imp.setSlice(current);
return values;
}
private ImageStatistics getLineStatistics(Roi roi, ImageProcessor ip, int measurements, Calibration cal) {
ImagePlus imp = new ImagePlus("", ip);
imp.setRoi(roi);
ProfilePlot profile = new ProfilePlot(imp);
double[] values = profile.getProfile();
ImageProcessor ip2 = new FloatProcessor(values.length, 1, values);
return ImageStatistics.getStatistics(ip2, measurements, cal);
}
}
|