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package ij.plugin;
import ij.plugin.*;
import ij.*;
import ij.process.*;
import ij.gui.*;
import ij.measure.*;
import ij.plugin.filter.Analyzer;
import ij.util.Tools;
import java.awt.*;
/** Implements the Image/Stacks/Plot Z-axis Profile command,
which plots the selection mean gray value versus slice number.
*/
public class ZAxisProfiler implements PlugIn, Measurements, PlotMaker {
private static String[] choices = {"time", "z-axis"};
private static String choice = choices[0];
private boolean showingDialog;
private ImagePlus imp;
private boolean isPlotMaker;
private boolean timeProfile;
private boolean firstTime = true;
private String options;
/** Returns a Plot of the selection mean gray value versus slice number. */
public static Plot getPlot(ImagePlus imp) {
return getPlot(imp, "time");
}
/** Returns a Plot of the selection mean versus slice number for the
specified hyperstack, where 'options' can be "time" or "z-axis". */
public static Plot getPlot(ImagePlus imp, String options) {
ZAxisProfiler zap = new ZAxisProfiler();
zap.imp = imp;
zap.options = options;
zap.isPlotMaker = true;
Plot plot = zap.getPlot();
return plot;
}
public void run(String arg) {
imp = IJ.getImage();
if (imp.getStackSize()<2) {
IJ.error("ZAxisProfiler", "This command requires a stack.");
return;
}
isPlotMaker = true;
Plot plot = getPlot();
if (plot!=null) {
if (isPlotMaker)
plot.setPlotMaker(this);
plot.show();
}
}
public Plot getPlot() {
Roi roi = imp.getRoi();
ImageProcessor ip = imp.getProcessor();
double minThreshold = ip.getMinThreshold();
double maxThreshold = ip.getMaxThreshold();
float[] y;
boolean hyperstack = imp.isHyperStack();
if (hyperstack)
y = getHyperstackProfile(imp, minThreshold, maxThreshold);
else
y = getZAxisProfile(imp, minThreshold, maxThreshold);
if (y==null)
return null;
float[] x = new float[y.length];
String xAxisLabel = showingDialog&&choice.equals(choices[0])?"Frame":"Slice";
Calibration cal = imp.getCalibration();
double calFactor = 1.0;
double origin = -1;
if (cal.scaled()) {
if (timeProfile) {
calFactor = (float) cal.frameInterval;
boolean zeroInterval = calFactor==0;
if (zeroInterval)
calFactor = 1;
else
origin = 0;
String timeUnit = zeroInterval?"Frame":"["+cal.getTimeUnit()+"]";
xAxisLabel = timeUnit;
} else {
calFactor = (float) cal.pixelDepth;
boolean zeroDepth = calFactor==0;
if (zeroDepth)
calFactor = 1;
else
origin = cal.zOrigin;
String depthUnit = zeroDepth?"Slice":"["+cal.getZUnit()+"]";
xAxisLabel = depthUnit;
}
}
for (int i=0; i<x.length; i++)
x[i] = (float)((i-origin)*calFactor);
String title;
if (roi!=null) {
Rectangle r = roi.getBounds();
title = imp.getTitle()+"-"+r.x+"-"+r.y;
} else
title = imp.getTitle()+"-0-0";
//String xAxisLabel = showingDialog&&choice.equals(choices[0])?"Frame":"Slice";
Plot plot = new Plot(title, xAxisLabel, "Mean", x, y);
boolean useConnectedCircles = x.length<=60; //not LINE but CONNECTED_CIRCLES
if (useConnectedCircles)
plot.setStyle(0, "black, gray, 1, connected");
plot.setColor(Color.black);
double ymin = ProfilePlot.getFixedMin();
double ymax = ProfilePlot.getFixedMax();
if (!(ymin==0.0 && ymax==0.0)) {
double[] a = Tools.getMinMax(x);
double xmin=a[0]; double xmax=a[1];
plot.setLimits(xmin, xmax, ymin, ymax);
} else {
double[] a = Tools.getMinMax(y);
ymin = a[0]; ymax = a[1];
}
//draw a blue vertical line for the current stack position (in live mode)
int pos = imp.getCurrentSlice();
if (hyperstack) {
if (timeProfile)
pos = imp.getT();
else
pos = imp.getZ();
}
double xx = (pos - 1 - origin)*calFactor;
if (!useConnectedCircles) { //For a line plot, the frame starts and ends at the first and last point
if (pos==1) //shift 1 pxl towards the center to avoid hiding the blue line hidden behind the frame
xx += calFactor*Math.min(0.4, x.length*0.7/(double)plot.getDrawingFrame().width);
else if (pos==x.length)
xx -= calFactor*Math.min(0.4, x.length*0.7/(double)plot.getDrawingFrame().width);
}
if (firstTime) //don't draw the line for the first time, create an (invisible) PlotObject
xx = Double.NaN; //(otherwise, the number of PlotObjects would change, breaking the Plot.COPY_EXTRA_OBJECTS)
plot.setColor(Color.blue);
plot.drawLine(xx, ymin-10*(ymax-ymin), xx, ymax+10*(ymax-ymin));
plot.setColor(Color.black);
plot.setLineWidth(1);
firstTime = false;
return plot;
}
public ImagePlus getSourceImage() {
return imp;
}
private float[] getHyperstackProfile(ImagePlus imp, double minThreshold, double maxThreshold) {
Roi roi = imp.getRoi();
int slices = imp.getNSlices();
int frames = imp.getNFrames();
int c = imp.getC();
int z = imp.getZ();
int t = imp.getT();
int size = slices;
if (firstTime)
timeProfile = slices==1 && frames>1;
if (options==null && slices>1 && frames>1 && (!isPlotMaker ||firstTime)) {
showingDialog = true;
GenericDialog gd = new GenericDialog("Profiler");
gd.addChoice("Profile", choices, choice);
gd.showDialog();
if (gd.wasCanceled())
return null;
choice = gd.getNextChoice();
timeProfile = choice.equals(choices[0]);
}
if (options!=null)
timeProfile = frames>1 && !options.contains("z");
if (timeProfile)
size = frames;
else
size = slices;
float[] values = new float[size];
Calibration cal = imp.getCalibration();
ResultsTable rt = new ResultsTable();
Analyzer analyzer = new Analyzer(imp, rt);
int measurements = Analyzer.getMeasurements();
boolean showResults = !isPlotMaker && measurements!=0 && measurements!=LIMIT;
measurements |= MEAN;
if (showResults) {
if (!Analyzer.resetCounter())
return null;
}
ImageStack stack = imp.getStack();
boolean showProgress = size>400 || stack.isVirtual();
for (int i=1; i<=size; i++) {
if (showProgress)
IJ.showProgress(i,size);
int index = 1;
if (timeProfile)
index = imp.getStackIndex(c, z, i);
else
index = imp.getStackIndex(c, i, t);
ImageProcessor ip = stack.getProcessor(index);
if (minThreshold!=ImageProcessor.NO_THRESHOLD)
ip.setThreshold(minThreshold,maxThreshold,ImageProcessor.NO_LUT_UPDATE);
ip.setRoi(roi);
ImageStatistics stats = ImageStatistics.getStatistics(ip, measurements, cal);
analyzer.saveResults(stats, roi);
values[i-1] = (float)stats.mean;
}
if (showResults)
rt.show("Results");
return values;
}
private float[] getZAxisProfile(ImagePlus imp, double minThreshold, double maxThreshold) {
Roi roi = imp.getRoi();
ImageStack stack = imp.getStack();
if (firstTime) {
int slices = imp.getNSlices();
int frames = imp.getNFrames();
timeProfile = slices==1 && frames>1;
}
int size = stack.size();
boolean showProgress = size>400 || stack.isVirtual();
float[] values = new float[size];
Calibration cal = imp.getCalibration();
ResultsTable rt = new ResultsTable();
Analyzer analyzer = new Analyzer(imp, rt);
int measurements = Analyzer.getMeasurements();
boolean showResults = !isPlotMaker && measurements!=0 && measurements!=LIMIT;
boolean showingLabels = firstTime && showResults && ((measurements&LABELS)!=0 || (measurements&SLICE)!=0);
measurements |= MEAN;
if (showResults) {
if (!Analyzer.resetCounter())
return null;
}
boolean isLine = roi!=null && roi.isLine();
int current = imp.getCurrentSlice();
for (int i=1; i<=size; i++) {
if (showProgress)
IJ.showProgress(i,size);
if (showingLabels)
imp.setSlice(i);
ImageProcessor ip = stack.getProcessor(i);
if (ip==null) {
IJ.log("ZAxisProfiler: stack.getProcessor("+i+") returned null ("+stack.getClass().getName()+","+ imp+")");
values[i-1] = Float.NaN;
continue;
}
if (minThreshold!=ImageProcessor.NO_THRESHOLD)
ip.setThreshold(minThreshold,maxThreshold,ImageProcessor.NO_LUT_UPDATE);
ip.setRoi(roi);
ImageStatistics stats = null;
if (isLine)
stats = getLineStatistics(roi, ip, measurements, cal);
else
stats = ImageStatistics.getStatistics(ip, measurements, cal);
analyzer.saveResults(stats, roi);
values[i-1] = (float)stats.mean;
}
if (showResults)
rt.show("Results");
if (showingLabels)
imp.setSlice(current);
return values;
}
private ImageStatistics getLineStatistics(Roi roi, ImageProcessor ip, int measurements, Calibration cal) {
ImagePlus imp = new ImagePlus("", ip);
imp.setRoi(roi);
ProfilePlot profile = new ProfilePlot(imp);
double[] values = profile.getProfile();
ImageProcessor ip2 = new FloatProcessor(values.length, 1, values);
return ImageStatistics.getStatistics(ip2, measurements, cal);
}
}
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