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/*=========================================================================
*
* Copyright Insight Software Consortium
*
* Licensed under the Apache License, Version 2.0 (the "License");
* you may not use this file except in compliance with the License.
* You may obtain a copy of the License at
*
* http://www.apache.org/licenses/LICENSE-2.0.txt
*
* Unless required by applicable law or agreed to in writing, software
* distributed under the License is distributed on an "AS IS" BASIS,
* WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
* See the License for the specific language governing permissions and
* limitations under the License.
*
*=========================================================================*/
#ifndef itkThresholdMaximumConnectedComponentsImageFilter_h
#define itkThresholdMaximumConnectedComponentsImageFilter_h
#include "itkBinaryThresholdImageFilter.h"
#include "itkConnectedComponentImageFilter.h"
#include "itkRelabelComponentImageFilter.h"
#include "itkMinimumMaximumImageCalculator.h"
#include "itkCastImageFilter.h"
namespace itk
{
/**\class ThresholdMaximumConnectedComponentsImageFilter
* \brief Finds the threshold value of an image based on maximizing the number
* of objects in the image that are larger than a given minimal size.
*
* \par
* This method is based on Topological Stable State Thresholding to
* calculate the threshold set point. This method is particularly
* effective when there are a large number of objects in a microscopy
* image. Compiling in Debug mode and enable the debug flag for this
* filter to print debug information to see how the filter focuses in
* on a threshold value. Please see the Insight Journal's MICCAI 2005
* workshop for a complete description. References are below.
*
* \par Parameters
* The MinimumObjectSizeInPixels parameter is controlled through the class
* Get/SetMinimumObjectSizeInPixels() method. Similar to the standard
* itk::BinaryThresholdImageFilter the Get/SetInside and Get/SetOutside values
* of the threshold can be set. The GetNumberOfObjects() and
* GetThresholdValue() methods return the number of objects above the
* minimum pixel size and the calculated threshold value.
*
* \par Automatic Thresholding in ITK
* There are multiple methods to automatically calculate the threshold
* intensity value of an image. As of version 4.0, ITK has a
* Thresholding ( ITKThresholding ) module which contains numerous
* automatic thresholding methods.implements two of these. Topological
* Stable State Thresholding works well on images with a large number
* of objects to be counted.
*
* \par References:
* 1) Urish KL, August J, Huard J. "Unsupervised segmentation for myofiber
* counting in immunoflourescent images". Insight Journal.
* ISC/NA-MIC/MICCAI Workshop on Open-Source Software (2005)
* Dspace handle: https://hdl.handle.net/1926/48
* 2) Pikaz A, Averbuch, A. "Digital image thresholding based on topological
* stable-state". Pattern Recognition, 29(5): 829-843, 1996.
*
* \par
* Questions: email Ken Urish at ken.urish(at)gmail.com
* Please cc the itk list serve for archival purposes.
*
* \ingroup ITKConnectedComponents
*/
template< typename TInputImage, typename TOutputImage = TInputImage >
class ITK_TEMPLATE_EXPORT ThresholdMaximumConnectedComponentsImageFilter:
public ImageToImageFilter< TInputImage, TOutputImage >
{
public:
/** Standard class typedefs. */
typedef ThresholdMaximumConnectedComponentsImageFilter Self;
typedef ImageToImageFilter< TInputImage, TOutputImage > Superclass;
typedef SmartPointer< Self > Pointer;
typedef SmartPointer< const Self > ConstPointer;
/** Method for creation through the object factory. */
itkNewMacro(Self);
/** Run-time type information (and related methods). */
itkTypeMacro(ThresholdMaximumConnectedComponentsImageFilter,
ImageToImageFilter);
/** Typedef to describe the type of pixel. */
typedef typename TInputImage::PixelType PixelType;
typedef typename TOutputImage::PixelType OutputPixelType;
/** The pixel type must support comparison operators. */
itkConceptMacro( PixelTypeComparable, ( Concept::Comparable< PixelType > ) );
/**
* Set the minimum pixel area used to count objects on the
* image. Thus, only objects that have a pixel area greater than the
* minimum pixel area will be counted as an object in the
* optimization portion of this filter. Essentially, it eliminates
* noise from being counted as an object. The default value is
* zero. */
itkSetMacro(MinimumObjectSizeInPixels, unsigned int);
itkGetConstMacro(MinimumObjectSizeInPixels, unsigned int);
/**
* The following Set/Get methods are for the binary threshold
* function. This class automatically calculates the lower threshold
* boundary. The upper threshold boundary, inside value, and outside
* value can be defined by the user, however the standard values
* are used as default if not set by the user.
* The default value of the: Inside value is the maximum pixel type
* intensity.
* Outside value is the minimum pixel
* type intensity.
* Upper threshold boundary is the
* maximum pixel type intensity.
*/
itkSetMacro(InsideValue, OutputPixelType);
itkSetMacro(OutsideValue, OutputPixelType);
itkSetMacro(UpperBoundary, PixelType);
itkGetConstMacro(InsideValue, OutputPixelType);
itkGetConstMacro(OutsideValue, OutputPixelType);
itkGetConstMacro(UpperBoundary, PixelType);
/**
* Returns the number of objects in the image. This information is
* only valid after the filter has executed. Useful if your counting
* something */
itkGetConstMacro(NumberOfObjects, SizeValueType);
/**
* Returns the automatic threshold setpoint. This information is
* only valid after the filter has executed. */
itkGetConstMacro(ThresholdValue, PixelType);
/** Some additional typedefs. */
typedef TInputImage InputImageType;
typedef typename InputImageType::ConstPointer InputImagePointer;
typedef typename InputImageType::RegionType InputImageRegionType;
typedef typename InputImageType::PixelType InputImagePixelType;
/** Some additional typedefs. */
typedef TOutputImage OutputImageType;
typedef typename OutputImageType::Pointer OutputImagePointer;
typedef typename OutputImageType::RegionType OutputImageRegionType;
typedef typename OutputImageType::PixelType OutputImagePixelType;
protected:
ThresholdMaximumConnectedComponentsImageFilter();
~ThresholdMaximumConnectedComponentsImageFilter() ITK_OVERRIDE {}
void PrintSelf(std::ostream & os, Indent indent) const ITK_OVERRIDE;
void GenerateData(void) ITK_OVERRIDE;
/**
* Runs a series of filters that thresholds the image,
* dilates/erodes for edge enhancement, and counts the number of
* relabeled connected components */
SizeValueType ComputeConnectedComponents();
private:
/** Typedef for filter pixel type. */
typedef unsigned int FilterPixelType;
itkStaticConstMacro(ImageDimension,
unsigned int,
TInputImage::ImageDimension);
typedef itk::Image< FilterPixelType, itkGetStaticConstMacro(ImageDimension) >
FilterImageType;
typedef typename FilterImageType::Pointer FilterImagePointer;
ITK_DISALLOW_COPY_AND_ASSIGN(ThresholdMaximumConnectedComponentsImageFilter);
//
// Binary Threshold Filter
//
typedef BinaryThresholdImageFilter< InputImageType, OutputImageType >
ThresholdFilterType;
//
// Connected Components Filter
//
typedef ConnectedComponentImageFilter< OutputImageType, FilterImageType >
ConnectedFilterType;
//
// Relabeled Components Filter
//
typedef RelabelComponentImageFilter< FilterImageType, FilterImageType >
RelabelFilterType;
//
// Minimum maximum calculator
//
typedef MinimumMaximumImageCalculator< InputImageType > MinMaxCalculatorType;
//
// Declare member variables for the filters of the internal pipeline.
//
typename ThresholdFilterType::Pointer m_ThresholdFilter;
typename ConnectedFilterType::Pointer m_ConnectedComponent;
typename RelabelFilterType::Pointer m_LabeledComponent;
typename MinMaxCalculatorType::Pointer m_MinMaxCalculator;
// Variables defined by the user
unsigned int m_MinimumObjectSizeInPixels;
// Binary threshold variables
OutputPixelType m_OutsideValue;
OutputPixelType m_InsideValue;
PixelType m_LowerBoundary;
PixelType m_UpperBoundary;
// Filter variables
PixelType m_ThresholdValue;
SizeValueType m_NumberOfObjects;
};
} // end namespace itk
#ifndef ITK_MANUAL_INSTANTIATION
#include "itkThresholdMaximumConnectedComponentsImageFilter.hxx"
#endif
#endif
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