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#include <cstdio>
#include <cstring>
#include <string>
#include <cstdlib>
#include <vector>
#include "Utils.h"
#include "LabelledCEdge.h"
#include <fstream>
#define MAX 1024
using namespace std;
struct NodeSeq {
char *seq;
int node;
NodeSeq(char *initSeq, int initNode) : seq(initSeq), node(initNode) { }
bool operator== (const NodeSeq& rhs) const { return (strcmp(seq, rhs.seq) == 0); }
bool operator< (const NodeSeq& rhs) const { return (strcmp(seq, rhs.seq) < 0); }
};
int count_nb_lines( FILE* file )
{
int ch, number_of_lines = 0;
while (EOF != (ch=getc(file)))
if ('\n' == ch)
number_of_lines++;
// Set the cursor back to the begining of the file.
rewind(file);
// Don't care if the last line has a '\n' or not. We over-estimate it.
return number_of_lines + 1;
}
void readEdgeFile( char* nodes_fname, vector<LabelledCEdge>& edges )
{
FILE *edge_file = open_file(nodes_fname);
char* buffer = new char[100 * MAX];
char* u = new char[MAX];
char* v = new char[MAX];
char* label = new char[MAX];
edges.reserve(count_nb_lines(edge_file));
while ( fgets(buffer, 100 * MAX, edge_file) != NULL )
{
char* p;
// outgoing node
p = strtok( buffer, "\t\n" );
strcpy( u, p );
// incoming node
p = strtok( NULL, "\t\n" );
strcpy( v, p );
// edge label
p = strtok( NULL, "\t\n" );
strcpy(label, p);
edges.push_back( LabelledCEdge( atoi(u), atoi(v), label ) );
}
sort( edges.begin(), edges.end() );
delete [] buffer;
delete [] u;
delete [] v;
delete [] label;
fclose(edge_file);
}
// rev_seq needs to be global! let's make sure we allocate only once
char *rev_seq = NULL;
char *revComp(char *seq)
{
int size = strlen(seq);
if (rev_seq == NULL)
rev_seq = new char[size + 1];
for (int i = size - 1; i >= 0; i--)
rev_seq[(size - 1) - i] = complement(seq[i]);
rev_seq[size] = '\0';
return rev_seq;
}
int findNode(char *query, vector<NodeSeq>& nodes)
{
vector<NodeSeq>::iterator low, low_r;
char *query_r = revComp(query);
// The value of "x" in NodeSeq(query, x) doesn't matter
low = lower_bound(nodes.begin(), nodes.end(), NodeSeq(query, 0));
low_r = lower_bound(nodes.begin(), nodes.end(), NodeSeq(query_r, 0));
if (low != nodes.end() && *low == NodeSeq(query, 0))
return low->node;
if (low_r != nodes.end() && *low_r == NodeSeq(query_r, 0))
return low_r->node;
return -1;
}
void readAbundanceFile (char* abundanceFileName, std::vector<double> &abundances) {
auto abundanceFile = std::ifstream(abundanceFileName);
if (!abundanceFile) {
std::fprintf(stderr, "Error opening %s\n", abundanceFileName);
exit(1);
}
double abundance = 0;
while (abundanceFile >> abundance) {
abundances.push_back(abundance);
}
}
int findEdge(vector<LabelledCEdge>& allEdges, LabelledCEdge e)
{
vector<LabelledCEdge>::iterator low;
low = lower_bound(allEdges.begin(), allEdges.end(), e);
if (low != allEdges.end())
return low - allEdges.begin();
fprintf(stderr, "inconsistent graph!");
return -1;
}
// Move this to CEdge
LabelledCEdge reverse(LabelledCEdge e)
{
char *label = new char[3];
label[0] = e.label[1] == 'F' ? 'R' : 'F';
label[1] = e.label[0] == 'F' ? 'R' : 'F';
label[2] = '\0';
return LabelledCEdge(e.getSecond(), e.getFirst(), label);
}
void errorRemoval(vector<LabelledCEdge>& allEdges, int nbNodes, double cutoff, vector<bool>& removed, const vector<double> &abundances)
{
int offset = 0;
for ( int src = 0 ; src < nbNodes; src++ ) {
// Count the number of edges (they are ordered in all_edges) of node src
int size = 0;
while (offset + size < (int) allEdges.size() && allEdges[offset + size].getFirst() == src)
size++;
// Compute the sum of coverage for each direction
double sum_F = 0, sum_R = 0;
for (int k = 0; k < size; k++) {
if (!removed[offset + k]) {
int target = allEdges[offset + k].getSecond();
if (allEdges[offset + k].label[0] == 'F')
sum_F += abundances[target];
if (allEdges[offset + k].label[0] == 'R')
sum_R += abundances[target];
}
}
// Remove the edges with relative coverage below cutoff
for (int k = 0; k < size; k++) {
if (!removed[offset + k]) {
int target = allEdges[offset + k].getSecond();
double count = abundances[target];
double ratio;
ratio = count / sum_F;
if (allEdges[offset + k].label[0] == 'F' && ratio < cutoff) {
removed[offset + k] = true;
removed[findEdge(allEdges, reverse(allEdges[offset + k]))] = true;
}
ratio = count / sum_R;
if (allEdges[offset + k].label[0] == 'R' && ratio < cutoff) {
removed[offset + k] = true;
removed[findEdge(allEdges, reverse(allEdges[offset + k]))] = true;
}
}
}
offset += size;
}
}
int main( int argc, char** argv )
{
string base_name = "graph";
if ( argc < 4 )
{
fprintf( stderr, "Wrong number of arguments!\n" );
fprintf( stderr, "Usage: ./error_removal edge_file unitig_abundance_file cutoff [base_name]\n" );
return 0;
}
if ( argc == 5 )
{
base_name = argv[4];
}
double cutoff = atof( argv[3] );
// Read edge file
vector<LabelledCEdge> allEdges;
readEdgeFile(argv[1], allEdges );
// Read abundance file
vector<double> abundances;
abundances.reserve(allEdges.size()); //over-estimating, but it is ok
readAbundanceFile(argv[2], abundances); //read the abundances
vector<bool> removed(allEdges.size(), false);
errorRemoval(allEdges, (int)abundances.size(), cutoff, removed, abundances);
int nb_removed = 0;
FILE *output = fopen((base_name + ".edges").c_str(), "w");
FILE *removed_out = fopen((base_name + "_removed.edges").c_str(), "w");
for (int i = 0 ; i < (int)allEdges.size(); i++)
if (!removed[i])
fprintf(output, "%d\t%d\t%s\n", allEdges[i].getFirst(), allEdges[i].getSecond(), allEdges[i].label);
else
{
fprintf(removed_out, "%d\t%d\t%s\n", allEdges[i].getFirst(), allEdges[i].getSecond(), allEdges[i].label);
nb_removed++;
}
fclose(output);
fclose(removed_out);
fprintf(stdout, "%d out of %d edges removed\n", nb_removed, (int)allEdges.size());
return 0;
}
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