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/*
This file is a part of KMC software distributed under GNU GPL 3 licence.
The homepage of the KMC project is http://sun.aei.polsl.pl/kmc
Authors: Marek Kokot
Version: 3.1.1
Date : 2019-05-19
*/
#ifndef _CHECK_KMER_H
#define _CHECK_KMER_H
#include "config.h"
#include "kmer.h"
#include "../kmc_api/mmer.h"
#include <string>
template<unsigned SIZE>
class CKmerCheck
{
CConfig& config;
const CKMC_header& header;
const CInputDesc& input_desc;
CKmer<SIZE> mask;
uint64 max_prefix;
uint32 record_size;
FILE* prefix_file = nullptr, *suffix_file = nullptr;
std::unique_ptr<uchar[]> rec;
uint32 check_in_suffix_file(uint64 lower, uint64 upper, CKmer<SIZE>& kmer_suffix)
{
if (!upper)
{
return 0;
}
upper--;
if (upper < lower)
return 0;
uint32 cutoff_range = input_desc.cutoff_max - input_desc.cutoff_min;
auto read_at = [](FILE* file, uint64 pos, uchar* &tmp, uint32 record_size, uint32 counter_size) -> CKmer<SIZE>
{
my_fseek(file, pos, SEEK_SET);
fread(tmp, 1, record_size, file);
CKmer<SIZE> res;
res.load(tmp, record_size - counter_size);
return res;
};
while (upper >= lower)
{
uint64 middle = (upper + lower) / 2;
uchar* tmp = rec.get();
auto middle_suffix = read_at(suffix_file, 4 + record_size * middle, tmp, record_size, header.counter_size);
if (middle_suffix < kmer_suffix)
{
lower = middle + 1;
}
else if (kmer_suffix < middle_suffix)
{
upper = middle - 1;
}
else
{
uint32 counter = 0;
for (uint32 i = 0; i < header.counter_size; ++i)
{
counter += (((uint32)*tmp++) << (i << 3));
}
if (counter - input_desc.cutoff_min < cutoff_range)
{
return counter;
}
break;
}
}
return 0;
}
void get_lower_upper(uint64 prefix, CKmer<SIZE>& kmer, uint64& lower, uint64& upper)
{
if (!header.IsKMC2())
{
uint64 pos = 4 + sizeof(uint64)*prefix;
my_fseek(prefix_file, pos, SEEK_SET);
fread(&lower, sizeof(uint64), 1, prefix_file);
if (prefix == max_prefix)
upper = header.total_kmers;
else
fread(&upper, sizeof(uint64), 1, prefix_file);
}
else
{
uint32 sig_len = header.signature_len;
CMmer cur_mmr(sig_len);
uint32 pos = header.kmer_len * 2 - 2;
for (uint32 i = 0; i < sig_len; ++i)
{
cur_mmr.insert(kmer.get_2bits(pos));
pos -= 2;
}
CMmer min_mmr(cur_mmr);
for (uint32 i = sig_len; i < header.kmer_len; ++i)
{
cur_mmr.insert(kmer.get_2bits(pos));
pos -= 2;
if (cur_mmr < min_mmr)
min_mmr = cur_mmr;
}
uint32 signature = min_mmr.get();
uint64 map_size = ((1 << 2 * header.signature_len) + 1) * sizeof(uint32);
my_fseek(prefix_file, 0ULL - (8 + header.header_offset + map_size) + signature * sizeof(uint32), SEEK_END);
uint32 prefix_array = 0;
uint32 prefix_arry_size = (1 << 2 * header.lut_prefix_len)*(uint32)sizeof(uint64);
fread(&prefix_array, sizeof(uint32), 1, prefix_file);
uint64 prefix_pos = 4 + prefix_arry_size*prefix_array + sizeof(uint64)*prefix;
my_fseek(prefix_file, prefix_pos, SEEK_SET);
fread(&lower, sizeof(uint64), 1, prefix_file);
fread(&upper, sizeof(uint64), 1, prefix_file);
}
}
public:
CKmerCheck(const CKMC_header& header, const CInputDesc& input_desc) :
config(CConfig::GetInstance()),
header(header),
input_desc(input_desc)
{
mask.set_n_1((header.kmer_len - header.lut_prefix_len) * 2);
std::string file_src = input_desc.file_src;
prefix_file = fopen((file_src + ".kmc_pre").c_str(), "rb");
if (!prefix_file)
{
std::cerr << "Error: cannot open file : " << (file_src + ".kmc_pre") << "\n";
exit(1);
}
file_src = input_desc.file_src;
suffix_file = fopen((file_src + ".kmc_suf").c_str(), "rb");
if (!suffix_file)
{
std::cerr << "Error: cannot open file : " << (file_src + ".kmc_suf") << "\n";
exit(1);
}
setvbuf(prefix_file, NULL, _IOFBF, (1ULL << 26));
setvbuf(suffix_file, NULL, _IOFBF, (1ULL << 26));
max_prefix = (1 << (2 * header.lut_prefix_len)) - 1;
record_size = (header.kmer_len - header.lut_prefix_len) / 4 + header.counter_size;
rec = std::make_unique<uchar[]>(record_size);
}
~CKmerCheck()
{
fclose(prefix_file);
fclose(suffix_file);
}
uint32 CheckKmer(CKmer<SIZE> kmer)
{
uint64 prefix = kmer.remove_suffix((header.kmer_len - header.lut_prefix_len) * 2);
uint64 lower = 0, upper = 0;
get_lower_upper(prefix, kmer, lower, upper);
kmer.mask(mask);
return check_in_suffix_file(lower, upper, kmer);
}
bool CheckKmer()
{
const std::string& kmer = config.check_params.kmer;
if (kmer.length() != header.kmer_len)
{
std::cerr << "Error: invalid k-mer length\n";
exit(1);
}
char codes[255];
for (uint32 i = 0; i < 255; ++i)
codes[i] = -1;
codes['A'] = codes['a'] = 0;
codes['C'] = codes['c'] = 1;
codes['G'] = codes['g'] = 2;
codes['T'] = codes['t'] = 3;
CKmer<SIZE> _kmer;
uint64 prefix = 0;
_kmer.clear();
for (uint32 i = 0; i < header.lut_prefix_len; ++i)
{
char d = codes[(uchar)kmer[i]];
if (d < 0)
{
std::cerr << "Error: invalid k-mer format\n";
exit(1);
}
prefix <<= 2;
prefix += d;
_kmer.SHL_insert_2bits(d);
}
for (uint32 i = header.lut_prefix_len; i<header.kmer_len; ++i)
{
char d = codes[(uchar)kmer[i]];
if (d < 0)
{
std::cerr << "Error: invalid k-mer format\n";
exit(1);
}
_kmer.SHL_insert_2bits(d);
}
uint64 lower = 0, upper = 0;
get_lower_upper(prefix, _kmer, lower, upper);
_kmer.mask(mask);
uint32 counter = check_in_suffix_file(lower, upper, _kmer);
std::cout << counter << "\n";
return true;
}
};
#endif
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