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/*
This file is a part of KMC software distributed under GNU GPL 3 licence.
The homepage of the KMC project is http://sun.aei.polsl.pl/kmc
Authors: Sebastian Deorowicz, Agnieszka Debudaj-Grabysz, Marek Kokot
Version: 3.2.4
Date : 2024-02-09
*/
#include "splitter.h"
//************************************************************************************************************
// CSplitter class - splits kmers into bins according to their signatures
//************************************************************************************************************
//uint32 CSplitter::MAX_LINE_SIZE = 1 << 14;
uint32 CSplitter::MAX_LINE_SIZE = 1 << 16;
//----------------------------------------------------------------------------------
// Assigns queues
CSplitter::CSplitter(CKMCParams &Params, CKMCQueues &Queues)
{
//mm = Queues.mm;
file_type = Params.file_type;
both_strands = Params.both_strands;
bin_part_queue = Queues.bpq.get();
pmm_reads = Queues.pmm_reads.get();
kmer_len = Params.kmer_len;
signature_len = Params.signature_len;
mem_part_pmm_bins = Params.mem_part_pmm_bins;
mem_part_pmm_reads = Params.mem_part_pmm_reads;
s_mapper = Queues.s_mapper.get();
part = nullptr;
// Prepare encoding of symbols
for (int i = 0; i < 256; ++i)
codes[i] = -1;
codes['A'] = codes['a'] = 0;
codes['C'] = codes['c'] = 1;
codes['G'] = codes['g'] = 2;
codes['T'] = codes['t'] = 3;
n_reads = 0;
homopolymer_compressed = Params.homopolymer_compressed;
ntHashEstimator = Queues.ntHashEstimator.get();
}
void CSplitter::InitBins(CKMCParams &Params, CKMCQueues &Queues)
{
n_bins = Params.n_bins;
uint32 buffer_size = Params.bin_part_size;
// Create objects for all bin
bins.resize(n_bins);
for (uint32 i = 0; i < n_bins; ++i)
{
bins[i] = std::make_unique<CKmerBinCollector>(Queues, Params, buffer_size, i);
}
}
//----------------------------------------------------------------------------------
// Parse long read, header_merker is '@' or '>'
bool CSplitter::GetSeqLongRead(char *seq, uint32 &seq_size, uchar header_marker)
{
uint32 pos = 0;
//long read may or may not contain header
if (part_pos == 0 && part[0] == header_marker)
{
++n_reads;
for (; part[part_pos] != '\n' && part[part_pos] != '\r'; ++part_pos)
;
}
while (pos < mem_part_pmm_reads && part_pos < part_size)
seq[pos++] = codes[part[part_pos++]];
seq_size = pos;
if (part_pos < part_size)
part_pos -= kmer_len - 1;
return true;
}
//----------------------------------------------------------------------------------
// Return a single record from FASTA/FASTQ data
bool CSplitter::GetSeq(char *seq, uint32 &seq_size, ReadType read_type)
{
if (part_pos >= part_size)
return false;
uchar c = 0;
uint32 pos = 0;
if (file_type == InputType::FASTA || file_type == InputType::KMC)
{
if (read_type == ReadType::long_read)
return GetSeqLongRead(seq, seq_size, '>');
if (curr_read_len == 0)
{
// Title
c = part[part_pos++];
if (c != '>')
return false;
++n_reads;
for (; part_pos < part_size;)
{
c = part[part_pos++];
if (c < 32) // newliners
break;
}
if (part_pos >= part_size)
return false;
c = part[part_pos++];
if (c >= 32 || c == part[part_pos - 2]) //read may be empty
part_pos--;
else if (part_pos >= part_size)
return false;
// Sequence
for (; part_pos < part_size && pos < mem_part_pmm_reads;)
{
c = part[part_pos++];
if (c < 32) // newliners
break;
seq[pos++] = codes[c];
}
seq_size = pos;
if (part_pos >= part_size)
return true;
curr_read_len = pos;
if (pos >= mem_part_pmm_reads) // read is too long to fit into out buff, it will be splitted into multiple buffers
{
part_pos -= kmer_len - 1;
return true;
}
}
else // we are inside read
{
// Sequence
for (; part_pos < part_size && pos < mem_part_pmm_reads;)
{
c = part[part_pos++];
if (c < 32) // newliners
break;
seq[pos++] = codes[c];
}
seq_size = pos;
if (part_pos >= part_size)
return true;
curr_read_len += pos - kmer_len + 1;
if (pos >= mem_part_pmm_reads) // read is too long to fit into out buff, it will be splitted into multiple buffers
{
part_pos -= kmer_len - 1;
return true;
}
}
curr_read_len = 0;
//end of last record
if (part_pos >= part_size)
return true;
if (part[part_pos++] >= 32)
part_pos--;
else if (part_pos >= part_size)
return true;
}
else if (file_type == InputType::FASTQ)
{
if (read_type == ReadType::long_read)
return GetSeqLongRead(seq, seq_size, '@');
if (curr_read_len == 0)
{
// Title
c = part[part_pos++];
if (c != '@')
return false;
++n_reads;
for (; part_pos < part_size;)
{
c = part[part_pos++];
if (c < 32) // newliners
break;
}
if (part_pos >= part_size)
return false;
c = part[part_pos++];
if (c >= 32 || c == part[part_pos - 2]) //read may be empty
part_pos--;
else if (part_pos >= part_size)
return false;
// Sequence
for (; part_pos < part_size && pos < mem_part_pmm_reads;)
{
c = part[part_pos++];
if (c < 32) // newliners
break;
seq[pos++] = codes[c];
}
if (part_pos >= part_size)
return false;
seq_size = pos;
curr_read_len = pos;
if (pos >= mem_part_pmm_reads) // read is too long to fit into out buff, it will be splitted into multiple buffers
{
part_pos -= kmer_len - 1;
return true;
}
}
else // we are inside read
{
// Sequence
for (; part_pos < part_size && pos < mem_part_pmm_reads;)
{
c = part[part_pos++];
if (c < 32) // newliners
break;
seq[pos++] = codes[c];
}
if (part_pos >= part_size)
return false;
seq_size = pos;
curr_read_len += pos - kmer_len + 1;
if (pos >= mem_part_pmm_reads) // read is too long to fit into out buff, it will be splitted into multiple buffers
{
part_pos -= kmer_len - 1;
return true;
}
}
c = part[part_pos++];
if (c >= 32)
part_pos--;
else if (part_pos >= part_size)
return false;
// Plus
c = part[part_pos++];
if (part_pos >= part_size)
return false;
if (c != '+')
return false;
for (; part_pos < part_size;)
{
c = part[part_pos++];
if (c < 32) // newliners
break;
}
if (part_pos >= part_size)
return false;
c = part[part_pos++];
if (c >= 32 || c == part[part_pos - 2]) //qual may be empty
part_pos--;
else if (part_pos >= part_size)
return false;
// Quality
part_pos += curr_read_len; //skip quality
curr_read_len = 0;
if (part_pos >= part_size)
return false;
c = part[part_pos++];
//end of last record
if (part_pos >= part_size)
return true;
//may be additional EOL character
if (part[part_pos++] >= 32)
part_pos--;
else if (part_pos >= part_size)
return true;
}
else if (file_type == InputType::MULTILINE_FASTA)
{
if (part[part_pos] == '>')//need to ommit header
{
++n_reads;
for (; part_pos < part_size && part[part_pos] != '\n' && part[part_pos] != '\r'; ++part_pos);//find EOF
++part_pos;
if (part[part_pos] == '\n' || part[part_pos] == '\r')
++part_pos;
}
for (; part_pos < part_size && pos < mem_part_pmm_reads && part[part_pos] != '>';)
{
seq[pos++] = codes[part[part_pos++]];
}
seq_size = pos;
if (part_pos < part_size && part[part_pos] != '>')//need to copy last k-1 kmers
{
part_pos -= kmer_len - 1;
}
return true;
}
else if (file_type == InputType::BAM)
{
while (true)
{
if (part_pos >= part_size)
return false;
int32_t block_size;
read_int32_t(block_size, part, part_pos);
uint64_t start_pos = part_pos;
part_pos += 8;
uint32_t bin_mq_nl;
read_uint32_t(bin_mq_nl, part, part_pos);
uint32_t l_read_name = (bin_mq_nl & ((1 << 8) - 1));
uint32_t flag_nc;
read_uint32_t(flag_nc, part, part_pos);
uint32_t n_cigar_op = flag_nc & ((1ul << 16) - 1);
int32_t l_seq;
read_int32_t(l_seq, part, part_pos);
part_pos += 12;
uint32_t flags = flag_nc >> 16;
bool exclude_read = ((flags >> 8) & 1) || ((flags >> 11) & 1); //TODO: I think that is the way samtools filter out some reads (secondary and supplementary)
part_pos += l_read_name; // skip read name
part_pos += 4 * n_cigar_op;
if (!exclude_read)
{
bool is_rev_comp = (flags >> 4) & 1;
if (!both_strands && is_rev_comp) //if read is reversed and kmc was run to count all (not only canonical) kmers read must be transformed back
{
//static const char rev_maping[] = "=TGMCRSVAWYHKDBN";
static const char rev_maping[] = { char(-1), char(3), char(2), char(-1), char(1), char(-1), char(-1), char(-1), char(0), char(-1), char(-1), char(-1), char(-1), char(-1), char(-1), char(-1)};// "=TGMCRSVAWYHKDBN";
uint32 n_bytes = l_seq / 2;
uint64_t pos_after = pos + l_seq;
pos = pos_after;
for (uint32_t ii = 0; ii < n_bytes; ++ii)
{
unsigned char byte = part[part_pos++];
seq[--pos_after] = rev_maping[byte >> 4];
seq[--pos_after] = rev_maping[byte & 15];
}
if (l_seq & 1) //odd
{
unsigned char byte = part[part_pos++];
seq[--pos_after] = rev_maping[byte >> 4];
}
}
else
{
static const char maping[] = { char(-1), char(0), char(1), char(-1), char(2), char(-1), char(-1), char(-1), char(3), char(-1), char(-1), char(-1), char(-1), char(-1), char(-1), char(-1) };//"=ACMGRSVTWYHKDBN";
uint32 n_bytes = l_seq / 2;
for (uint32_t ii = 0; ii < n_bytes; ++ii)
{
unsigned char byte = part[part_pos++];
seq[pos++] = maping[byte >> 4];
seq[pos++] = maping[byte & 15];
}
if (l_seq & 1) //odd
{
unsigned char byte = part[part_pos++];
seq[pos++] = maping[byte >> 4];
}
}
seq_size = pos;
}
else
{
part_pos += (l_seq + 1) / 2;
}
//move to next record
uint64_t readed = part_pos - start_pos;
uint64_t remaining = block_size - readed;
part_pos += remaining;
if (!exclude_read) //if readed successfuly return
{
++n_reads;
return true;
}
}
}
return (c == '\n' || c == '\r');
}
//----------------------------------------------------------------------------------
void CSplitter::HomopolymerCompressSeq(char* seq, uint32 &seq_size)
{
if (seq_size <= 1)
return;
uint32 read_pos = 1;
uint32 write_pos = 0;
for (; read_pos < seq_size; ++read_pos)
if (seq[read_pos] != seq[write_pos])
seq[++write_pos] = seq[read_pos];
seq_size = write_pos + 1;
}
//----------------------------------------------------------------------------------
// Calculate statistics of m-mers
void CSplitter::CalcStats(uchar* _part, uint64 _part_size, ReadType read_type, uint32* _stats)
{
part = _part;
part_size = _part_size;
part_pos = 0;
char *seq;
uint32 seq_size;
pmm_reads->reserve(seq);
uint32 signature_start_pos;
CMmer current_signature(signature_len), end_mmer(signature_len);
uint32 i;
uint32 len;//length of extended kmer
while (GetSeq(seq, seq_size, read_type))
{
if (homopolymer_compressed)
HomopolymerCompressSeq(seq, seq_size);
i = 0;
len = 0;
while (i + kmer_len - 1 < seq_size)
{
bool contains_N = false;
//building first signature after 'N' or at the read begining
for (uint32 j = 0; j < signature_len; ++j, ++i)
if (seq[i] < 0)//'N'
{
contains_N = true;
break;
}
//signature must be shorter than k-mer so if signature contains 'N', k-mer will contains it also
if (contains_N)
{
++i;
continue;
}
len = signature_len;
signature_start_pos = i - signature_len;
current_signature.insert(seq + signature_start_pos);
end_mmer.set(current_signature);
for (; i < seq_size; ++i)
{
if (seq[i] < 0)//'N'
{
if (len >= kmer_len)
_stats[current_signature.get()] += 1 + len - kmer_len;
len = 0;
++i;
break;
}
end_mmer.insert(seq[i]);
if (end_mmer < current_signature)//signature at the end of current k-mer is lower than current
{
if (len >= kmer_len)
{
_stats[current_signature.get()] += 1 + len - kmer_len;
len = kmer_len - 1;
}
current_signature.set(end_mmer);
signature_start_pos = i - signature_len + 1;
}
else if (end_mmer == current_signature)
{
current_signature.set(end_mmer);
signature_start_pos = i - signature_len + 1;
}
else if (signature_start_pos + kmer_len - 1 < i)//need to find new signature
{
_stats[current_signature.get()] += 1 + len - kmer_len;
len = kmer_len - 1;
//looking for new signature
++signature_start_pos;
//building first signature in current k-mer
end_mmer.insert(seq + signature_start_pos);
current_signature.set(end_mmer);
for (uint32 j = signature_start_pos + signature_len; j <= i; ++j)
{
end_mmer.insert(seq[j]);
if (end_mmer <= current_signature)
{
current_signature.set(end_mmer);
signature_start_pos = j - signature_len + 1;
}
}
}
++len;
}
}
if (len >= kmer_len)//last one in read
_stats[current_signature.get()] += 1 + len - kmer_len;
}
pmm_reads->free(seq);
}
//----------------------------------------------------------------------------------
// Process the reads from the given FASTQ file part, but only for k-mer occurence estimation
bool CSplitter::ProcessReadsOnlyEstimate(uchar* _part, uint64 _part_size, ReadType read_type)
{
part = _part;
part_size = _part_size;
part_pos = 0;
char* seq;
uint32 seq_size;
pmm_reads->reserve(seq);
while (GetSeq(seq, seq_size, read_type))
ntHashEstimator->Process(seq, seq_size);
pmm_reads->free(seq);
return true;
}
//----------------------------------------------------------------------------------
// Process the reads from the given FASTQ file part
bool CSplitter::ProcessReads(uchar *_part, uint64 _part_size, ReadType read_type)
{
part = _part;
part_size = _part_size;
part_pos = 0;
char *seq;
uint32 seq_size;
pmm_reads->reserve(seq);
uint32 signature_start_pos;
CMmer current_signature(signature_len), end_mmer(signature_len);
uint32 bin_no;
uint32 i;
uint32 len;//length of extended kmer
while (GetSeq(seq, seq_size, read_type))
{
if (ntHashEstimator)
ntHashEstimator->Process(seq, seq_size);
if (homopolymer_compressed)
HomopolymerCompressSeq(seq, seq_size);
//if (file_type != multiline_fasta && file_type != fastq) //read conting moved to GetSeq
// n_reads++;
i = 0;
len = 0;
while (i + kmer_len - 1 < seq_size)
{
bool contains_N = false;
//building first signature after 'N' or at the read begining
for (uint32 j = 0; j < signature_len; ++j, ++i)
if (seq[i] < 0)//'N'
{
contains_N = true;
break;
}
//signature must be shorter than k-mer so if signature contains 'N', k-mer will contains it also
if (contains_N)
{
++i;
continue;
}
len = signature_len;
signature_start_pos = i - signature_len;
current_signature.insert(seq + signature_start_pos);
end_mmer.set(current_signature);
for (; i < seq_size; ++i)
{
if (seq[i] < 0)//'N'
{
if (len >= kmer_len)
{
bin_no = s_mapper->get_bin_id(current_signature.get());
bins[bin_no]->PutExtendedKmer(seq + i - len, len);
}
len = 0;
++i;
break;
}
end_mmer.insert(seq[i]);
if (end_mmer < current_signature)//signature at the end of current k-mer is lower than current
{
if (len >= kmer_len)
{
bin_no = s_mapper->get_bin_id(current_signature.get());
bins[bin_no]->PutExtendedKmer(seq + i - len, len);
len = kmer_len - 1;
}
current_signature.set(end_mmer);
signature_start_pos = i - signature_len + 1;
}
else if (end_mmer == current_signature)
{
current_signature.set(end_mmer);
signature_start_pos = i - signature_len + 1;
}
else if (signature_start_pos + kmer_len - 1 < i)//need to find new signature
{
bin_no = s_mapper->get_bin_id(current_signature.get());
bins[bin_no]->PutExtendedKmer(seq + i - len, len);
len = kmer_len - 1;
//looking for new signature
++signature_start_pos;
//building first signature in current k-mer
end_mmer.insert(seq + signature_start_pos);
current_signature.set(end_mmer);
for (uint32 j = signature_start_pos + signature_len; j <= i; ++j)
{
end_mmer.insert(seq[j]);
if (end_mmer <= current_signature)
{
current_signature.set(end_mmer);
signature_start_pos = j - signature_len + 1;
}
}
}
++len;
if (len == kmer_len + 255) //one byte is used to store counter of additional symbols in extended k-mer
{
bin_no = s_mapper->get_bin_id(current_signature.get());
bins[bin_no]->PutExtendedKmer(seq + i + 1 - len, len);
i -= kmer_len - 2;
len = 0;
break;
}
}
}
if (len >= kmer_len)//last one in read
{
bin_no = s_mapper->get_bin_id(current_signature.get());
bins[bin_no]->PutExtendedKmer(seq + i - len, len);
}
}
pmm_reads->free(seq);
return true;
}
//----------------------------------------------------------------------------------
// Process the reads from the given FASTQ file part in small k optimization mode
template<typename COUNTER_TYPE>
bool CSplitter::ProcessReadsSmallK(uchar *_part, uint64 _part_size, ReadType read_type, CSmallKBuf<COUNTER_TYPE>& small_k_buf)
{
part = _part;
part_size = _part_size;
part_pos = 0;
char *seq;
uint32 seq_size;
int omit_next_n_kmers;
CKmer<1> kmer_str, kmer_rev, kmer_can;
uint32 i;
CKmer<1> kmer_mask;
pmm_reads->reserve(seq);
kmer_mask.set_n_1(2 * kmer_len);
uint32 kmer_len_shift = (kmer_len - 1) * 2;
if (both_strands)
while (GetSeq(seq, seq_size, read_type))
{
if (homopolymer_compressed)
HomopolymerCompressSeq(seq, seq_size);
//if (file_type != multiline_fasta)
// n_reads++;
// Init k-mer
kmer_str.clear();
kmer_rev.clear();
// Process first k-1 symbols of a read
uint32 str_pos = kmer_len_shift - 2;
uint32 rev_pos = 2;
omit_next_n_kmers = 0;
for (i = 0; i < kmer_len - 1; ++i, str_pos -= 2, rev_pos += 2)
{
if (seq[i] < 0)
{
seq[i] = 0;
omit_next_n_kmers = i + 1;
}
kmer_str.set_2bits(seq[i], str_pos);
kmer_rev.set_2bits(3 - seq[i], rev_pos);
}
// Process next part of a read
for (; i < seq_size; ++i)
{
if (seq[i] < 0) // N in a read
{
seq[i] = 0;
omit_next_n_kmers = kmer_len; // Mark how many symbols to ommit to get the next kmer without any N
}
kmer_str.SHL_insert_2bits(seq[i]);
kmer_str.mask(kmer_mask);
kmer_rev.SHR_insert_2bits(3 - seq[i], kmer_len_shift);
// If necessary ommit next symbols
if (omit_next_n_kmers > 0)
{
omit_next_n_kmers--;
continue;
}
// Find canonical kmer representation
kmer_can = (kmer_str < kmer_rev) ? kmer_str : kmer_rev;
++small_k_buf.buf[kmer_can.data];
++total_kmers;
}
}
else
while (GetSeq(seq, seq_size, read_type))
{
if (homopolymer_compressed)
HomopolymerCompressSeq(seq, seq_size);
//if (file_type != multiline_fasta)
// n_reads++;
// Init k-mer
kmer_str.clear();
// Process first k-1 symbols of a read
uint32 str_pos = kmer_len_shift - 2;
omit_next_n_kmers = 0;
for (i = 0; i < kmer_len - 1; ++i, str_pos -= 2)
{
if (seq[i] < 0)
{
seq[i] = 0;
omit_next_n_kmers = i + 1;
}
kmer_str.set_2bits(seq[i], str_pos);
}
// Process next part of a read
for (; i < seq_size; ++i)
{
if (seq[i] < 0) // N in a read
{
seq[i] = 0;
omit_next_n_kmers = kmer_len; // Mark how many symbols to ommit to get the next kmer without any N
}
kmer_str.SHL_insert_2bits(seq[i]);
kmer_str.mask(kmer_mask);
// If necessary ommit next symbols
if (omit_next_n_kmers > 0)
{
omit_next_n_kmers--;
continue;
}
++small_k_buf.buf[kmer_str.data];
++total_kmers;
}
}
pmm_reads->free(seq);
return true;
}
//----------------------------------------------------------------------------------
// Finish the processing of input file
void CSplitter::Complete()
{
for (auto& bin : bins)
if (bin)
bin->Flush();
}
//************************************************************************************************************
// CWSplitter class - wrapper for multithreading purposes
//************************************************************************************************************
//----------------------------------------------------------------------------------
// Constructor
CWSplitter::CWSplitter(CKMCParams &Params, CKMCQueues &Queues)
{
pq = Queues.part_queue.get();
bpq = Queues.bpq.get();
pmm_fastq = Queues.pmm_fastq.get();
spl = std::make_unique<CSplitter>(Params, Queues);
spl->InitBins(Params, Queues);
}
//----------------------------------------------------------------------------------
// Execution
void CWSplitter::operator()()
{
// Splitting parts
while (!pq->completed())
{
uchar *part;
uint64 size;
ReadType read_type;
if (pq->pop(part, size, read_type))
{
spl->ProcessReads(part, size, read_type);
pmm_fastq->free(part);
}
}
spl->Complete();
bpq->mark_completed();
spl->GetTotal(n_reads);
spl.reset();
}
//----------------------------------------------------------------------------------
// Destructor
CWSplitter::~CWSplitter()
{
}
//----------------------------------------------------------------------------------
// Return statistics
void CWSplitter::GetTotal(uint64 &_n_reads)
{
if (spl)
spl->GetTotal(n_reads);
_n_reads = n_reads;
}
//************************************************************************************************************
// CWStatsSplitter class - wrapper for multithreading purposes
//************************************************************************************************************
//----------------------------------------------------------------------------------
// Constructor
CWStatsSplitter::CWStatsSplitter(CKMCParams &Params, CKMCQueues &Queues)
{
spq = Queues.stats_part_queue.get();
pmm_fastq = Queues.pmm_fastq.get();
pmm_stats = Queues.pmm_stats.get();
progressObserver = Params.progressObserver;
spl = std::make_unique<CSplitter>(Params, Queues);
signature_len = Params.signature_len;
pmm_stats->reserve(stats);
fill_n(stats, (1 << signature_len * 2) + 1, 0);
}
//----------------------------------------------------------------------------------
// Destructor
CWStatsSplitter::~CWStatsSplitter()
{
pmm_stats->free(stats);
}
//----------------------------------------------------------------------------------
// Execution
void CWStatsSplitter::operator()()
{
// Splitting parts
while (!spq->completed())
{
uchar *part;
uint64 size;
ReadType read_type;
if (spq->pop(part, size, read_type))
{
spl->CalcStats(part, size, read_type, stats);
progressObserver->Step();
pmm_fastq->free(part);
}
}
spl.reset();
}
//----------------------------------------------------------------------------------
void CWStatsSplitter::GetStats(uint32* _stats)
{
uint32 size = (1 << signature_len * 2) + 1;
for (uint32 i = 0; i < size; ++i)
_stats[i] += stats[i];
}
//************************************************************************************************************
// CWSmallKSplitter class - wrapper for multithreading purposes
//************************************************************************************************************
//----------------------------------------------------------------------------------
// Constructor
template <typename COUNTER_TYPE> CWSmallKSplitter<COUNTER_TYPE>::CWSmallKSplitter(CKMCParams &Params, CKMCQueues &Queues)
{
pq = Queues.part_queue.get();
pmm_fastq = Queues.pmm_fastq.get();
pmm_small_k = Queues.pmm_small_k_buf.get();
kmer_len = Params.kmer_len;
spl = std::make_unique<CSplitter>(Params, Queues);
}
//----------------------------------------------------------------------------------
// Destructor
template <typename COUNTER_TYPE> CWSmallKSplitter<COUNTER_TYPE>::~CWSmallKSplitter()
{
}
//----------------------------------------------------------------------------------
// Execution
template <typename COUNTER_TYPE> void CWSmallKSplitter<COUNTER_TYPE>::operator()()
{
pmm_small_k->reserve(small_k_buf.buf);
memset(small_k_buf.buf, 0, (1ull << 2 * kmer_len) * sizeof(*small_k_buf.buf));
// Splitting parts
while (!pq->completed())
{
uchar *part;
uint64 size;
ReadType read_type;
if (pq->pop(part, size, read_type))
{
spl->ProcessReadsSmallK(part, size, read_type, small_k_buf);
pmm_fastq->free(part);
}
}
spl->Complete();
spl->GetTotal(n_reads);
total_kmers = spl->GetTotalKmers();
spl.reset();
}
//----------------------------------------------------------------------------------
// Return statistics
template <typename COUNTER_TYPE> void CWSmallKSplitter<COUNTER_TYPE>::GetTotal(uint64 &_n_reads)
{
if (spl)
spl->GetTotal(n_reads);
_n_reads = n_reads;
}
//************************************************************************************************************
// CWSplitter class - wrapper for multithreading purposes
//************************************************************************************************************
//----------------------------------------------------------------------------------
// Constructor
CWEstimateOnlySplitter::CWEstimateOnlySplitter(CKMCParams& Params, CKMCQueues& Queues)
{
pq = Queues.part_queue.get();
pmm_fastq = Queues.pmm_fastq.get();
spl = std::make_unique<CSplitter>(Params, Queues);
}
//----------------------------------------------------------------------------------
// Execution
void CWEstimateOnlySplitter::operator()()
{
// Splitting parts
while (!pq->completed())
{
uchar* part;
uint64 size;
ReadType read_type;
if (pq->pop(part, size, read_type))
{
spl->ProcessReadsOnlyEstimate(part, size, read_type);
pmm_fastq->free(part);
}
}
spl->GetTotal(n_reads);
spl.reset();
}
//----------------------------------------------------------------------------------
// Destructor
CWEstimateOnlySplitter::~CWEstimateOnlySplitter()
{
}
//----------------------------------------------------------------------------------
// Return statistics
void CWEstimateOnlySplitter::GetTotal(uint64& _n_reads)
{
if (spl)
spl->GetTotal(n_reads);
_n_reads = n_reads;
}
//instantiate some templates
template bool CSplitter::ProcessReadsSmallK(uchar *_part, uint64 _part_size, ReadType read_type, CSmallKBuf<uint32>& small_k_buf);
template bool CSplitter::ProcessReadsSmallK(uchar *_part, uint64 _part_size, ReadType read_type, CSmallKBuf<uint64>& small_k_buf);
template class CWSmallKSplitter<uint32>;
template class CWSmallKSplitter<uint64>;
// ***** EOF
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