1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301
|
#!/usr/bin/perl
# Copyright [2015-2018] EMBL-European Bioinformatics Institute
#
# Licensed under the Apache License, Version 2.0 (the "License");
# you may not use this file except in compliance with the License.
# You may obtain a copy of the License at
#
# http://www.apache.org/licenses/LICENSE-2.0
#
# Unless required by applicable law or agreed to in writing, software
# distributed under the License is distributed on an "AS IS" BASIS,
# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
# See the License for the specific language governing permissions and
# limitations under the License.
use strict;
use File::Temp 'tempdir';
use Cwd;
use File::Path qw(make_path);
use Getopt::Long;
my $htslib_version = "1.5";
my $version = get_version();
my $help = "INSTALL.pl [-h|--help] [--prefix=filepath] [--static] [~/prefix/path]\n";
$help .= "--help (-h) - this help message\n";
$help .= "--prefix - Path to install Bio::DB::HTS.\n";
$help .= " Alternative to providing a path at the end of comandline\n";
$help .= " If neither are provided defaults to root install (requires root access)\n";
$help .= "--static - Build Bio::DB::HTS using the static libhts.a library and do not install htslib\n";
$help .= "--htslib_version - Build Bio::DB::HTS using the specified HTSlib version (Default ".$htslib_version.")\n";
my $cwd = system 'pwd';
my $opts = parse_options();
my $prefix_path;
$prefix_path = $opts->{'prefix'} if(exists($opts->{'prefix'}) && defined($opts->{'prefix'}));
$htslib_version = $opts->{'htslib_version'} if($opts->{'htslib_version'}) ;
my ($version_major,$version_minor,$version_revision) = split /\./, $htslib_version ;
# STEP 0: various dependencies
my $git = `which git`;
$git or die <<END;
'git' command not in path. Please install git and try again.
On Debian/Ubuntu systems you can do this with the command:
apt-get install git
END
`which cc` or die <<END;
'cc' command not in path. Please install it and try again.
On Debian/Ubuntu systems you can do this with the command:
apt-get install build-essential
END
`which make` or die <<END;
'make' command not in path. Please install it and try again.
On Debian/Ubuntu systems you can do this with the command:
apt-get install build-essential
END
# The following libraries are version number dependant for HTSlib
if( $version_major >= 2 || ($version_major==1 && $version_minor>=5) )
{
-e '/usr/include/lzma.h' or die <<END;
lzma.h library header not found in /usr/include. Please install it and try again.
On Debian/Ubuntu systems you can do this with the command:
apt-get install liblzma-dev
END
-e '/usr/include/bzlib.h' or die <<END;
zlib.h library header not found in /usr/include. Please install it and try again.
On Debian/Ubuntu systems you can do this with the command:
apt-get install libbz2-dev
END
}
else
{
-e '/usr/include/zlib.h' or die <<END;
zlib.h library header not found in /usr/include. Please install it and try again.
On Debian/Ubuntu systems you can do this with the command:
apt-get install zlib1g-dev
END
;
}
eval "require Bio::SeqFeature::Lite" or die <<END;
BioPerl does not seem to be installed. Please install it and try again.
On Debian/Ubuntu systems you can do this with the command:
apt-get install bioperl
On other systems use the CPAN shell:
perl -MCPAN -e 'install Bio::Perl'
END
;
# STEP 1: Create a clean directory for building
my $install_dir = tempdir(CLEANUP => 1);
info("Performing build in $install_dir");
# STEP 2: Download HTSlib
info("Checking out HTSlib v".$htslib_version);
chdir $install_dir;
my $htslib_archive = $htslib_version.".zip" ;
my $htslib_archive_url = "https://github.com/samtools/htslib/archive/".$htslib_version.".zip" ;
system "wget -O " . $htslib_archive . " " .$htslib_archive_url ;
if ( $? == -1 )
{
die "HTSlib fetch command failed: $!\n";
}
-f './'.$htslib_archive or die "Could not fetch HTSlib archive ".$htslib_archive_url ;
system "unzip ".$htslib_archive ;
system "mv htslib-$htslib_version htslib" ;
-d './htslib' or die "Unzip seems to have failed. Could not find $install_dir/htslib directory";
# STEP 3: Download Bio-DB-HTS
info("Fetching version $version of Bio-DB-HTS from GitHub");
chdir $install_dir;
my $biodbhts_archive = "$version.zip" ;
my $biodbhts_archive_url = "https://github.com/Ensembl/Bio-DB-HTS/archive/$version.zip" ;
system "wget -O " . $biodbhts_archive . " " .$biodbhts_archive_url ;
if ( $? == -1 )
{
die "Bio-DB-HTS fetch command failed: $!\n";
}
-f './'.$biodbhts_archive or die "Could not fetch Bio::DB::HTS archive ".$biodbhts_archive_url ;
system "unzip ".$biodbhts_archive ;
system "mv Bio-DB-HTS-$version Bio-DB-HTS" ;
-d './Bio-DB-HTS' or die "Unzip seems to have failed. Could not find $install_dir/Bio-DB-HTS directory";
# Step 4: Build libhts.a
info("Building HTSlib");
chdir "$install_dir/htslib";
# patch makefile
open my $in, '<','Makefile' or die "Couldn't open Makefile for reading: $!";
open my $out,'>','Makefile.new' or die "Couldn't open Makefile.new for writing: $!";
while (<$in>) {
chomp;
if (/^CFLAGS/ && !/-fPIC/) {
s/#.+//; # get rid of comments
$_ .= " -fPIC -Wno-unused -Wno-unused-result";
}
} continue {
print $out $_,"\n";
}
close $in;
close $out;
rename 'Makefile.new','Makefile' or die "Couldn't rename Makefile.new to Makefile: $!";
warn "***MAKE***\n";
system "make";
if(defined $prefix_path && !$opts->{'static'}) {
warn "***MAKE INSTALL***\n";
system "make install prefix=$prefix_path";
}
if($opts->{'static'}){
system "rm -f $install_dir/htslib/libhts.so*";
}
-e 'libhts.a' or die "Compile didn't complete. No libhts.a library file found";
# Step 5: Build Bio::DB::HTS
info("Building Bio::DB::HTS");
chdir "$install_dir/Bio-DB-HTS";
my $cmd;
if(defined $prefix_path) {
if(!$opts->{'static'}) {
$cmd = "env HTSLIB_DIR=$prefix_path/lib perl Build.PL --install_base=$prefix_path";
} else {
$cmd = "env HTSLIB_DIR=$install_dir/htslib perl Build.PL --install_base=$prefix_path";
}
} else {
$cmd = "env HTSLIB_DIR=$install_dir/htslib perl Build.PL";
}
if($opts->{'static'}) {
$cmd .= " --static=1";
}
warn "***CMD*** : $cmd\n";
system $cmd;
-e "./Build" or die "Build.PL didn't execute properly: no Build file found";
system "./Build";
`./Build test` =~ /Result: PASS/ or die "Build test failed. Not continuing";
# Step 6: Install
my $sudo = `which sudo`; chomp($sudo);
if(defined $prefix_path) {
info("Installing Bio::DB::HTS to $prefix_path.");
`./Build install` =~ /Installing.+?HTS\.pm/ or die "Couldn't install to $prefix_path: $?";
} elsif ($sudo) {
info("Installing Bio::DB::HTS using sudo. You will be asked for your password.");
info("If this step fails because sudo isn't installed, go back and run this script again as superuser.");
system "sudo ./Build install";;
} else {
info("Installing Bio::DB::HTS system wide without sudo.");
info("Make sure you have the right privileges.");
`./Build install` =~ /Installing.+?HTS\.pm/ or die "Couldn't install at system level without sudo: $?";
}
if($opts->{'static'}){
system "rm -f $install_dir/htslib/libhts.a";
}
# Step 7: Yay!
info("Bio::DB::HTS v$version is now installed.");
chdir '/';
exit 0;
sub parse_options {
my ($factory) = @_;
my %opts = ();
my $result = &GetOptions (
'static' => \$opts{'static'},
'prefix=s' => \$opts{'prefix'},
'htslib_version=s' => \$opts{'htslib_version'},
'h|help' => \$opts{'h'},
);
if($opts{'h'}){
warn $help,"\n";
exit(0);
}
if(defined($opts{'prefix'})){
$opts{'prefix'} = prefix_install($opts{'prefix'});
}elsif(!defined($opts{'prefix'}) && @ARGV > 0){
$opts{'prefix'} = prefix_install(shift(@ARGV));
}elsif(exists($ENV{PERL_LOCAL_LIB_ROOT}) && defined($ENV{PERL_LOCAL_LIB_ROOT})){
$opts{'prefix'} = prefix_install($ENV{PERL_LOCAL_LIB_ROOT});
}
$opts{'static'} = 0 if(!defined($opts{'static'}));
$opts{'static'} = $ENV{STATIC_HTS} if((!$opts{'static'}) &&
exists($ENV{STATIC_HTS}) && defined($ENV{STATIC_HTS}));
return \%opts;
}
sub info {
my $msg = shift;
chomp $msg;
print STDERR "\n*** $msg ***\n";
}
sub prefix_install {
my $prefix_path = shift;
if($prefix_path =~ s/^\~//) {
$prefix_path = $ENV{HOME}.$prefix_path
}
elsif($prefix_path !~ m|^/|) {
$prefix_path = getcwd().'/'.$prefix_path;
}
my $err;
unless(-e $prefix_path) {
make_path($prefix_path, {verbose => 1, error => \$err});
if (@$err) {
for my $diag (@$err) {
my ($dir, $message) = %$diag;
if ($dir eq '') { print "general error: $message\n"; }
else { print "problem creating dir $dir: $message\n"; }
}
}
}
return $prefix_path;
}
sub get_version {
use FindBin '$Bin';
my $version = `grep -m 1 "HTS::VERSION" $Bin/lib/Bio/DB/HTS.pm`;
$version =~ /(\d+?\.\d+?)\D/;
$version = $1;
use Carp;
croak "Couldn't get a meaningful library version: $version"
unless $version =~ /\d+?\.\d+?/;
return $version;
}
|