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package Bio::GMOD::DB::Adapter::Wormbase;
use base 'Bio::GMOD::DB::Adapter';
sub new {
my $class = shift;
my %arg = @_;
my $self = $class->SUPER::new(%arg);
return $self;
}
sub handle_unreserved_tags {
my $self = shift;
my ($feature,$uniquename,@unreserved_tags) = @_;
foreach my $tag (@unreserved_tags) {
next if $tag eq 'source';
next if $tag eq 'phase';
next if $tag eq 'seq_id';
next if $tag eq 'type';
next if $tag eq 'score';
next if $tag eq 'dbxref';
unless ($self->{const}{fp_cv_id} || $self->{const}{tried_fp_cv}){
$self->fp_cv("autocreated") unless($self->fp_cv());
my $sth = $self->dbh->prepare("SELECT cv_id FROM cv WHERE name='". $self->fp_cv() ."'");
# dgg: dropped 'autocreated' due to SO/auto conflicts for things like 'gene', 'chromosome'
# where SO and autocreated cvs are used primarily for type names in Bio/DB/Das/Chado
$sth->execute;
($self->{const}{fp_cv_id}) = $sth->fetchrow_array;
if(!$self->{const}{fp_cv_id} && $self->{'addpropertycv'}) {
# create cv entry
$self->{const}{fp_cv_id}= $self->nextoid('cv');
$self->print_cv( $self->{const}{fp_cv_id}, $self->fp_cv());
$self->nextoid('cv','++');
}
$self->{const}{tried_fp_cv} = 1;
}
# if (!$self->{const}{tried_fp_cv} and !$self->{const}{fp_cv_id}) {
# my $sth = $self->dbh->prepare("SELECT cv_id FROM cv WHERE name='". $self->fp_cv ."'");
# $sth->execute;
# ($self->{const}{fp_cv_id}) = $sth->fetchrow_array;
# $self->{const}{tried_fp_cv} = 1;
# }
## problems here with auto-added properties that clash with SO/other cvterms; cache-type?
my $property_cvterm_id = $self->cache('property',$tag);
# $property_cvterm_id = $self->cache('type',$tag) unless($property_cvterm_id); ## dgg; drop this?
unless ( $property_cvterm_id ) {
#check fp cv first; ## dgg drop this due to conflicts with SO type: autocreated
## my ($tag_cvterm); # == $property_cvterm_id
if ($self->{const}{fp_cv_id}) {
$self->{queries}{search_cvterm_id}->execute(
$tag,
$self->{const}{fp_cv_id}) ;
($property_cvterm_id) = $self->{queries}{search_cvterm_id}->fetchrow_array;
}
if ($property_cvterm_id) { #good, the term is already there
$self->cache('property',$tag,$property_cvterm_id);
} else { #bad! the term is not there for now we die with a helpful message
## dgg patch
if($self->{'addpropertycv'} && $self->{const}{fp_cv_id}) {
$property_cvterm_id= $self->nextoid('cvterm'); # $nextcvterm++;
my $dbxid= $self->nextoid('dbxref'); #$nextdbxref++;
my $cvid = $self->{const}{fp_cv_id};
my $dbxacc= "autocreated:$tag";
## bad to use gff_source_db id; use 'null' db
unless ($self->{const}{null_db}) {
my $sth = $self->dbh->prepare("SELECT db_id FROM db WHERE name='null'");
$sth->execute;
($self->{const}{null_db}) = $sth->fetchrow_array;
}
$self->print_dbx($dbxid,$self->{const}{null_db},$dbxacc,1,'\N');
$self->nextoid('dbxref','++');
$self->cache('dbxref',$dbxacc,$dbxid);
$self->print_cvterm($property_cvterm_id, $cvid, $tag, $dbxid);
$self->nextoid('cvterm','++');
$self->cache('property',$tag,$property_cvterm_id);
} else {
dbxref_error_message($tag) && die;
}
}
}
#moving on, add this to the featureprop table
my @values = map {$_->value} $feature->annotation->get_Annotations($tag);
my $rank=0;
foreach my $value (@values) {
if ( $self->constraint( name => 'featureprop_c1',
terms=> [ $self->cache('feature',$uniquename),
$self->cache('property',$tag),
$rank ] ) ) {
$self->print_fprop($self->nextoid('featureprop'),$self->cache('feature',$uniquename),$property_cvterm_id,$value,$rank);
$rank++;
$self->nextoid('featureprop','++'); # $nextfeatureprop++;
}
}
}
}
=head2 handle_CDS
=over
=item Usage
$obj->handle_CDS($feature_obj)
=item Function
This function stores CDS and UTR features in a temporary database
table for processing after the entire GFF3 file has be seen.
If the feature's parents do not correspond to the central dogma
(that is, gene -> transcript -> cds), then the method will return
false and the CDS or UTR feature will be inserted as is into
the database.
=item Returns
False if the feature doesn't belong to a central dogma gene,
otherwise nothing.
=item Arguments
A Bio::FeatureIO CDS or UTR object
=back
=cut
sub handle_CDS {
my $self = shift;
my $feat = shift;
my $dbh = $self->dbh;
# warn Dumper($feat);
my $feat_id = ($feat->annotation->get_Annotations('ID'))[0]->value
if ($feat && defined(($feat->annotation->get_Annotations('ID'))[0]));
my @feat_parents= map {$_->value}
$feat->annotation->get_Annotations('Parent')
if ($feat && defined(($feat->annotation->get_Annotations('Parent'))[0]));
#assume that an exon can have at most one grandparent (gene, operon)
my $parent_id = $self->cache('feature',$feat_parents[0]) if $feat_parents[0];
unless ($parent_id) {
warn "\n\nThere is a ".$feat->type->name." feature with no parent (ID:$feat_id) I think that is wrong!\n\n";
}
my $feat_grandparent = $self->cache('parent',$parent_id);
return 0 unless $feat_grandparent;
unless ($self->cds_db_exists()) {
$self->create_cds_db;
}
my $fmin = $feat->start; #check that this is interbase
my $fmax = $feat->end;
# my $object = safeFreeze $feat; ## original; dgg; was bad for argos perl lib; had real old FreezeThaw
## dgg this works, and doesnt need a new 3rd party perl module:
my $dumper = Data::Dumper->new ([[$feat]]);
$dumper->Indent(0)->Terse(1)->Purity(1);
my $object = $dumper->Dump;
my $feat_type = $feat->type->name;
##$feat_type= $feat_type->value if(ref $feat_type);
my $seq_id = $feat->seq_id; ## this is a ref->value !!
my $insert = qq/
INSERT INTO tmp_cds_handler (gff_id,seq_id,type,fmin,fmax,object)
VALUES (?,?,?,?,?,?)
/;
my $sth = $dbh->prepare($insert);
$sth->execute($feat_id,$seq_id,$feat_type,$fmin,$fmax,$object);
#get the value of the row just inserted
$sth = $dbh->prepare("SELECT currval('tmp_cds_handler_cds_row_id_seq')");
$sth->execute;
my ($cds_row_id) = $sth->fetchrow_array;
$sth = $dbh->prepare("INSERT INTO tmp_cds_handler_relationship (cds_row_id,parent_id,grandparent_id) VALUES (?,?,?)");
for my $parent (@feat_parents) {
$sth->execute($cds_row_id,$parent,$feat_grandparent);
}
return 1;
}
=head2 process_CDS
=over
=item Usage
my $feature_iterator = $obj->process_CDS()
=item Function
Retrieves CDS and UTR objects from a temporary database table and
does necessary conversion to exon and polypeptide features and
returns a feature iterator to let the bulk loader process them
=item Returns
A Bio::GMOD::Adaptor::FeatureIterator object
=item Arguments
None.
=back
=cut
sub process_CDS {
my $self = shift;
# $self->dbh->commit && die;
return unless $self->cds_db_exists;
my $dbh = $self->dbh;
#get one of the features from the database(!)
# print Dumper($self);
# die;
my $min_feat_query = "SELECT min(fmax) FROM tmp_cds_handler";
my $sth = $dbh->prepare($min_feat_query);
$sth->execute;
my ($min_feat) = $sth->fetchrow_array;
my $cds_utr_query = qq/
SELECT distinct cds.gff_id,cds.object,cds.type,cds.fmin,cds.fmax, rel.grandparent_id
FROM tmp_cds_handler cds, tmp_cds_handler_relationship rel
WHERE rel.cds_row_id = cds.cds_row_id
AND rel.grandparent_id IN
(SELECT grandparent_id FROM tmp_cds_handler_relationship
WHERE cds_row_id IN
(SELECT cds_row_id FROM tmp_cds_handler WHERE fmax = ?))
ORDER BY cds.fmin,cds.gff_id
/;
$sth = $dbh->prepare($cds_utr_query);
$sth->execute($min_feat);
my %polypeptide;
my @feature_list;
my $grandparent;
#do stuff, create a list of features
while (my $feat_row = $sth->fetchrow_hashref) {
$grandparent = $$feat_row{ grandparent_id };
## dgg: Data::Dumper works and is easier on user (Data::Dumper part of sys perl lib) ##
##my ($feat_obj)= thaw $$feat_row{ object }; # original
my $objs = eval $$feat_row{ object }; if($@) { warn @$; }
my $feat_obj = $$objs[0];
my $type = $$feat_row{ type };
my $fmin = $$feat_row{ fmin };
my $fmax = $$feat_row{ fmax };
my @parents = map {$_->value}
$feat_obj->annotation->get_Annotations('Parent');
for my $parent_id (@parents) {
if ($type =~ /CDS/) {
#check for a polypeptide with for this parent
if ($polypeptide{ $parent_id }) {
#add to it if it exists
if ( $polypeptide{ $parent_id }->start > $fmin ) {
$polypeptide{ $parent_id }->start($fmin);
}
if ( $polypeptide{ $parent_id }->end < $fmax ) {
$polypeptide{ $parent_id }->end($fmax);
}
}
else {
#create it if it doesn't
my $polyp = Bio::SeqFeature::Annotated->new();
$polyp->start( $fmin );
$polyp->end( $fmax );
$polyp->strand( $feat_obj->strand );
$polyp->name( $parent_id.' polypeptide');
my $srcval= Bio::Annotation::SimpleValue->new(
ref($feat_obj->source)
? $feat_obj->source->value : $feat_obj->source);
$polyp->source( $srcval );
my $polyp_ac = Bio::Annotation::Collection->new();
$polyp_ac->add_Annotation( 'source', $srcval);
$polyp_ac->add_Annotation(
'Note',Bio::Annotation::SimpleValue->new(
'polypeptide feature inferred from GFF3 CDS feature'));
$polyp_ac->add_Annotation(
'Derives_from',Bio::Annotation::SimpleValue->new(
$parent_id));
$polyp_ac->add_Annotation(
'type',Bio::Annotation::OntologyTerm->new(
-term => Bio::Ontology::Term->new(-name=>'polypeptide')));
$polyp_ac->add_Annotation(
'seq_id',Bio::Annotation::SimpleValue->new(
$feat_obj->seq_id));
$polyp_ac->add_Annotation(
'phase',Bio::Annotation::SimpleValue->new('.'));
$polyp->annotation($polyp_ac);
$polypeptide{ $parent_id } = $polyp;
}
}
}
#create an exon feature (or add to an existing one)
my $merged_exon = 0;
for my $exon ( @feature_list ) {
next unless ($exon->type->name eq 'exon');
if ($exon->start == $fmax - 1 ) {
#this feature imideately precedes an existing exon, glue them together
$exon->start($fmin);
$exon = $self->_merge_annotations($exon, $feat_obj);
$merged_exon = 1;
}
if ($exon->end == $fmin -1 ) {
#this feature come right after an existing exon, glue them together
$exon->end($fmax);
$exon = $self->_merge_annotations($exon, $feat_obj);
$merged_exon = 1;
}
}
# if ($merged_exon) {
# print Dumper($_) for @feature_list;
# }
unless ($merged_exon) {
#convert the existing feature to an exon
my $ac = $feat_obj->annotation();
$ac->remove_Annotations('type');
$ac->add_Annotation('type',Bio::Annotation::OntologyTerm->new(
-term => Bio::Ontology::Term->new(-name=>'exon')));
$ac->add_Annotation('Note',Bio::Annotation::SimpleValue->new(
'Exon inferred from GFF3 ' .
$feat_obj->type->name .
' feature line'));
$feat_obj->annotation($ac);
push @feature_list, $feat_obj;
}
}
#add the polypeptides to the list
if ($self->noexon) {
#only return the polpeptides if noexon is set
@feature_list = values %polypeptide;
}
else {
push @feature_list, values %polypeptide;
}
#delete the features from the temp tables:
my $delete_query = qq/DELETE FROM tmp_cds_handler WHERE cds_row_id IN
(SELECT cds_row_id FROM tmp_cds_handler_relationship WHERE grandparent_id =?)
/;
$sth = $dbh->prepare($delete_query);
$sth->execute($grandparent);
$dbh->commit;
#return an iterator
if (@feature_list > 0) {
return Bio::GMOD::DB::Adapter::FeatureIterator->new(\@feature_list);
}
else {
return 0;
}
}
=head2 _merge_annotations
=over
=item Usage
$obj->_merge_annotations()
=item Function
Take two adjecent feature objects and merge their annotations
=item Returns
The merged feature object (which will be an exon feature)
=item Arguments
Two feature objects, with the existing exon first
=back
=cut
sub _merge_annotations {
my ($self, $exon, $obj2) = @_;
my $exon_ac = $exon->annotation;
my $obj2_ac = $obj2->annotation;
for my $key ( $obj2_ac->get_all_annotation_keys() ) {
my @values = $obj2_ac->get_Annotations($key);
if ($key eq 'type') {
$exon_ac->add_Annotation('Note',Bio::Annotation::SimpleValue->new(
'exon feature the result of two merged features in GFF3, one '.
'of which was a '.$obj2->type->name.' feature'));
}
elsif ( $key eq 'source'
or $key eq 'Parent'
or $key eq 'seq_id'
or $key eq 'phase'
or $key eq 'score' ) {
next;
}
else {
for my $value ( @values ) {
$exon_ac->add_Annotation($key,$value);
}
}
}
$exon->annotation($exon_ac);
return $exon;
}
=pod
my $iterator;
#so its time to process the most recent set of features and return an iterator
if (($feat_id && $self->{cdscache}{id} && $feat_id ne $self->{cdscache}{id})
or
($feat_parent && $self->{cdscache}{parent} && $feat_parent ne $self->{cdscache}{parent})
or
(!$self->{cdscache}{id} && !$self->{cdscache}{parent}) ) {
#this is a new cds feature so package up the old one to give back
if ($self->noexon) {
$iterator = Bio::GMOD::DB::Adapter::FeatureIterator->new(
$self->{cdscache}{polypeptide_obj}
);
}
elsif ($self->{cdscache}{polypeptide_obj}) {
push @{ $self->{cdscache}{feature_array} },
$self->{cdscache}{polypeptide_obj};
$iterator = Bio::GMOD::DB::Adapter::FeatureIterator->new(
\@{ $self->{cdscache}{feature_array} }
);
}
#now empty the caches and set parent/id
$self->{cdscache}{feature_array} = ();
$self->{cdscache}{polypeptide_obj} = '';
$self->{cdscache}{id} = $feat_id;
$self->{cdscache}{parent} = $feat_parent;
}
#get the current AnnotationCollection and change
# that is, convert CDS features to exon features
if ($feat && !$self->noexon) {
#check for existing created exons that but up against this feature
my $start = $feat->start;
my $stop = $feat->end;
my $appended_feature_flag = 0;
for my $cached_feat ( @{ $self->{cdscache}{feature_array} } ) {
if ($stop + 1 == $cached_feat->start) {
my $cached_ac = $cached_feat->annotation();
my $ac = $feat->annotation();
$ac->remove_Annotations('type');
$ac->add_Annotation('Note',Bio::Annotation::SimpleValue->new(
'Exon added to from an adjacent feature in GFF3'));
my @annot_list = $ac->get_Annotations;
for my $annot (@annot_list) {
$cached_ac->add_Annotation($annot);
}
$cached_feat->start($start);
$appended_feature_flag = 1;
}
elsif ( $start == $cached_feat->end + 1 ) {
my $cached_ac = $cached_feat->annotation();
my $ac = $feat->annotation();
$ac->remove_Annotations('type');
$ac->add_Annotation('Note',Bio::Annotation::SimpleValue->new(
'Exon added to from an adjacent feature in GFF3'));
my @annot_list = $ac->get_Annotations;
for my $annot (@annot_list) {
$cached_ac->add_Annotation($annot);
}
$cached_feat->end($stop);
$appended_feature_flag = 1;
}
}
unless ( $appended_feature_flag ) {
my $ac = $feat->annotation();
$ac->remove_Annotations('type');
$ac->add_Annotation('type',Bio::Annotation::OntologyTerm->new(
-term => Bio::Ontology::Term->new(-name=>'exon')));
$ac->add_Annotation('Note',Bio::Annotation::SimpleValue->new(
'Exon inferred from GFF3 ' .
$feat->type->name .
' feature line'));
$feat->annotation($ac);
}
}
if ($feat && !$self->{cdscache}{polypeptide_obj}) {
#polypeptide doesn't exist yet, so create it
my $polyp = Bio::SeqFeature::Annotated->new();
$polyp->start( $feat->start );
$polyp->end( $feat->end );
$polyp->strand( $feat->strand );
$polyp->name( $feat_parent.' polypeptide');
my $polyp_ac = Bio::Annotation::Collection->new();
$polyp_ac->add_Annotation('Note',Bio::Annotation::SimpleValue->new(
'polypeptide feature inferred from GFF3 CDS feature'));
$polyp_ac->add_Annotation('Derives_from',Bio::Annotation::SimpleValue->new(
$feat_parent));
$polyp_ac->add_Annotation('type',Bio::Annotation::OntologyTerm->new(
-term => Bio::Ontology::Term->new(-name=>'polypeptide')));
$polyp_ac->add_Annotation('seq_id',Bio::Annotation::SimpleValue->new(
$feat->seq_id));
$polyp->annotation($polyp_ac);
$self->{cdscache}{polypeptide_obj} = $polyp;
}
#check for bounds change on the existing polypeptide
elsif ( $feat
&& $self->{cdscache}{polypeptide_obj}->start > $feat->start
&& $feat->type->name =~ /CDS/ ) {
$self->{cdscache}{polypeptide_obj}->start($feat->start);
}
elsif ( $feat
&& $self->{cdscache}{polypeptide_obj}->end < $feat->end
&& $feat->type->name =~ /CDS/ ) {
$self->{cdscache}{polypeptide_obj}->end($feat->end);
}
push @{ $self->{cdscache}{feature_array} }, $feat if $feat;
return $iterator;
}
=cut
sub handle_parent {
my $self = shift;
my ($feature) = @_;
for my $p_anot ( $feature->annotation->get_Annotations('Parent') ) {
my $pname = $p_anot->value;
my $parent = $self->cache('feature',$pname);
die "\nno parent $pname;\nyou probably need to rerun the loader with the --recreate_cache option\n\n" unless $parent;
$self->cache('parent',$self->nextfeature,$parent);
$self->print_frel($self->nextoid('feature_relationship'),$self->nextfeature,$parent,$part_of);
$self->nextoid('feature_relationship','++'); # $nextfeaturerel++;
}
}
sub handle_derives_from {
my $self = shift;
my ($feature) = @_;
for my $p_anot ( $feature->annotation->get_Annotations('Derives_from') ) {
my $pname = $p_anot->value;
my $parent = $self->cache('feature',$pname);
die "no parent ".$pname unless $parent;
$self->cache('parent',$self->nextfeature,$parent);
$self->print_frel($self->nextoid('feature_relationship'),$self->nextfeature,$parent,$derives_from);
$self->nextoid('feature_relationship','++'); #$nextfeaturerel++;
}
}
sub handle_crud {
my $self = shift;
my $feature = shift;
my $force_delete = shift;
my ($op) = $feature->annotation->get_Annotations('CRUD');
$op = $op->value if defined($op);
if ($force_delete) {
$op = 'delete-all';
}
my ($name) = $feature->annotation->get_Annotations('Name');
if (!defined($name)) {
#try to get the name from the ID
($name) = $feature->annotation->get_Annotations('ID');
if (!defined($name)) {
#if it doesn't have a name, don't do anything
return 1;
}
}
$name = $name->value if ref($name);
my $type = ref($feature->type) ? $feature->type->name : $feature->type;
if ($op =~ /delete/) {
#determine if a single feature corresponds to what is in the gff line
#it is considered to be the same if the type, name (or synonym)
#and organism are the same
#this sql should be moved to the prepared sql hash after debugging is done
my $sql = "SELECT feature_id FROM feature
WHERE name = ? and type_id = ? and organism_id = ?";
my $delete_query_handle = $self->dbh->prepare($sql);
$delete_query_handle->execute($name,
$self->get_type($type),
$self->organism_id);
my $feature_id_arrayref = $delete_query_handle->fetchall_arrayref;
my $feature_id;
if (scalar @{$feature_id_arrayref} > 1 and $op ne 'delete-all') {
$self->throw("I can't figure out which feature to delete that corresponds to a feature with a name of $name, a type of $type and organism of ".$self->organism.". More than one feature match these criteria");
}
elsif (scalar @{$feature_id_arrayref} > 1) {
warn "Deleting all features with name $name, type $type and organism ".$self->organism."\n";
for my $id_row (@{$feature_id_arrayref}) {
my $feature_id = $$id_row[0];
$self->print_delete($$id_row[0]) if $feature_id;
}
return 1;
}
elsif (scalar @{$feature_id_arrayref} == 0) {
warn "Couldn't fined a matching feature with name $name, type $type and organism ".$self->organism."\n";
return 1;
# warn("Searching for a feature with the name $name to delete yielded nothing; checking synonyms...");
# $sql = "SELECT f.feature_id
# FROM feature f, feature_synonym fs, synonym s
# WHERE s.name = ? and
# s.synonym_id = fs.synonym_id and
# fs.feature_id = f.feature_id and
# f.type_id = ? and f.organism_id = ?";
# my $delete_by_syn_query_handle = $self->dbh->prepare($sql);
# $delete_by_syn_query_handle->execute($name,
# $self->get_type($type),
# $self->organism_id);
# $feature_id_arrayref = $delete_by_syn_query_handle->fetchall_arrayref;
# if (scalar @{$feature_id_arrayref} > 1) {
# $self->throw("I couldn't figure out which feature to delete when searching by synonym $name; I found more than one matching feature");
# }
# elsif (scalar @{$feature_id_arrayref} == 0) {
# $self->throw("I couldn't find a matching feature using either feature.name or synonym.name of $name and a type of $type and organism of ".$self->organism.". I can't go on... Bye.");
# }
# else {
# ($feature_id) = $$feature_id_arrayref[0];
# }
}
else {
$feature_id = $$feature_id_arrayref[0][0];
}
$self->print_delete($feature_id);
return 1;
}
elsif ($op eq 'replace' or $op eq 'update') {
$self->throw("The CRUD operation $op is not supported yet");
}
elsif ($op eq 'create') {
return 0; #nothing to do--create is the default
}
else {
$self->throw("I don't know what to do for the CRUD operation $op");
}
}
1;
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