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package SOI::GFF3Parser;
=head1 NAME
SOI::GFF3Parser
=head1 SYNOPSIS
perlSAX handler to parse soi xml
=head1 USAGE
=begin
my $parser = SOI::GFF3Parser->new([qw(property_type_map2method)]);
$parser->parse($yourGFF3File);
my $feature = $parser->feature; #get SOI::Feature obj (feature tree) from soi xml
=end
=cut
=head1 FEEDBACK
Email sshu@fruitfly.org
=cut
use strict;
use SOI::Feature;
use FileHandle;
use Carp;
=head1 FUNCTIONS
=cut
sub new {
my $class = shift;
my $self = {};
bless $self, $class;
my $file = shift;
$self->file($file);
my $types = shift;
if ($types) {
$types = [$types] unless (ref($types) eq 'ARRAY');
$self->{method_types} = $types;
}
return $self;
}
sub method_types {
my $self = shift;
if (@_) {
my $types = shift;
$types = [$types] unless (ref($types) eq 'ARRAY');
$self->{method_types} = $types;
}
return $self->{method_types};
}
sub file {
my $self = shift;
my $file = shift;
if ($file) {
my $fh = FileHandle->new("<$file") || confess("Failed to open file $file: $!");
$self->fh($fh);
$self->{file} = $file;
undef $self->{top_features};
undef $self->{feature_h};
}
return $self->{file};
}
sub fh {
my $self = shift;
$self->{fh} = shift if (@_);
return $self->{fh};
}
sub parse {
my $self = shift;
my $f = shift;
$self->file($f) if ($f);
my $tops = $self->fetch_object(); #no object id, return top objects
map{$_->set_depth(0)}@{$tops || []};
# return $tops;
}
#hard to implement get_next_object?
sub fetch_object {
my $self = shift;
my $oid = shift;
my $fh = $self->fh;
return $self->_parse($fh, $oid);
}
sub _last_meta {
my $self = shift;
$self->{last_meta} = shift if (@_);
return $self->{last_meta} || "";
}
sub _parse {
my $self = shift;
my $fh = shift;
my $oid = shift;
#my (%seq_h, %feat_h, @tops);
my @predefined = qw(ID Name Alias Parent Target Gap Note Dbxref Ontology_term);
while (my $line = <$fh>) {
chomp $line;
next unless ($line);
if ($line =~ /^\#\#/) {
my $last_meta = $self->_last_meta || "";
$self->_last_meta($line);
if ($line =~ /^\#{3}$/) {
if ($last_meta =~ /^\#\#FASTA/) {
#end of fasta seq
}
elsif ($oid) {
last; #end of feature for the oid requested (### better be at right level)
}
}
#rest meta NOT IMPLEMENTED
next;
}
if ($self->_last_meta =~ /^\#\#FASTA/) {
#get residue
next;
}
#only able to handle tab-delimited file for now
my @a = split/\t/, $line;
map{undef $_ if ($_ eq '.')}@a;
my $col9 = pop @a;
my ($src_seq,$source,$type,$start,$end,$score,$strand,$phase) = @a;
my (%magic_h, @props);
map {
my ($tag, $val) = split/\=/, $_;
if (grep{$tag eq $_}@predefined) {
my @v = (split/[,+]/, $val);
$magic_h{$tag} = \@v;
} else {
push @props, {type=>$tag,value=>$val};
}
}(split/\;\s*/, $col9);
my ($id) = @{$magic_h{ID} || []};
confess("A feature must have ID and type") unless ($id && $type);
my ($name) = @{$magic_h{Name} || []};
my $parent = $magic_h{Parent};
my $target = $magic_h{Target};
my ($gap) = @{$magic_h{Ga} || []};
push @props, {type=>'cigar',values=>$gap} if ($gap);
my ($note) = @{$magic_h{Note} || []};
push @props, {type=>'note', value=>$note} if ($note);
my $feature = SOI::Feature->new({uniquename=>$id,name=>$name,type=>$type});
confess("Feature ID: $id is duplicated") if (exists $self->{feature_h}->{$id});
$self->{feature_h}->{$id} = $feature;
if ($parent) {
map {
my $p = $self->{feature_h}->{$_};
#parent must appear first!!!
$p->add_node($feature);
}@{$parent || []};
} else {
push @{$self->{top_features}}, $feature;
}
if ($start && $end) {
confess("Locatable feature must have src and coord including strand: $line") unless ($src_seq && $strand);
my ($fmin, $fmax) = ($start - 1, $end);
$feature->src_seq($src_seq);
$feature->fmin($fmin);
$feature->fmax($fmax);
$feature->strand($strand);
}
if ($target) {
my $sstrand = $target->[3] || 1;
$feature->add_secondary_node(SOI::Feature->new({src_seq=>$target->[0], fmin=>$target->[1]-1, fmax=>$target->[2], strand=>$sstrand}));
}
&_set_synonyms($feature, $magic_h{Alias});
&_set_dbxrefs($feature, $magic_h{Dbxref});
&_set_ontology_terms($feature, $magic_h{Ontology_term});
$self->setup_properties($feature, \@props);
}
if ($oid) {
return $self->{feature_h}->{$oid};
}
return $self->{top_features};
}
sub _set_synonyms {
my $feature = shift;
my $syns = shift || return;
##??
}
sub _set_dbxrefs {
my $feature = shift;
my $dbxrefs = shift || return;
my @a;
map{my ($db, $acc) = (split/\:/);push @a, {dbname=>$db,accession=>$acc}}@$dbxrefs;
$feature->dbxrefs(\@a);
}
sub _set_ontology_terms {
my $feature = shift;
my $terms = shift || return;
my @a;
map{my ($db, $acc) = (split/\:/);push @a, {dbname=>$db,accession=>$acc}}@$terms;
$feature->ontologies(\@a);
}
sub setup_properties {
my $self = shift;
my $feature = shift;
my $props = shift;
my (@a, @m);
foreach my $p (@{$props || []}) {
if (grep{$p->{type} eq $_}@{$self->{method_types} || []}) {
push @m, $p;
} else {
push @a, $p;
}
}
$feature->properties(\@a) if (@a);
foreach my $m (@m) {
$feature->hash->{$m->{type}} = $m->{value};
}
}
=head2 feature
usage: my $feature = $self->feature;
returns: top node feature
=cut
sub feature {
my $self = shift;
return unless (@{$self->{top_features} || []});
return $self->{top_features}->[0];
}
sub features {
my $self = shift;
return $self->{top_features};
}
1;
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