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/////////////////////////////////////////////////////////////////////////////
// File: gnGBKSource.h
// Purpose: Implements gnBaseSource for GenBank sequences
// Description:
// Changes:
// Version: libGenome 0.5.1
// Author: Aaron Darling
// Modified by:
// Copyright: (c) Aaron Darling
// Licenses: See COPYING file for details
/////////////////////////////////////////////////////////////////////////////
#ifdef HAVE_CONFIG_H
#include "config.h"
#endif
#include "libGenome/gnFilter.h"
#include "libGenome/gnFeature.h"
#include "libGenome/gnGBKSource.h"
#include "libGenome/gnSourceSpec.h"
#include "libGenome/gnSourceHeader.h"
#include "libGenome/gnSourceQualifier.h"
#include "libGenome/gnLocation.h"
#include "libGenome/gnStringTools.h"
#include "libGenome/gnDebug.h"
#include "libGenome/gnStringQualifier.h"
#include <string>
#include <cstring>
using namespace std;
namespace genome {
gnGBKSource::gnGBKSource()
{
m_openString = "";
m_pFilter = gnFilter::proteinSeqFilter();
if(m_pFilter == NULL){
DebugMsg("Error using static sequence filters.");
}
}
gnGBKSource::gnGBKSource( const gnGBKSource& s ) : gnFileSource(s)
{
vector< gnFileContig* >::const_iterator iter = s.m_contigList.begin();
for( ; iter != s.m_contigList.end(); ++iter )
{
m_contigList.push_back( (*iter)->Clone() );
}
}
gnGBKSource::~gnGBKSource()
{
m_ifstream.close();
vector< gnFileContig* >::iterator iter = m_contigList.begin();
for( ; iter != m_contigList.end(); ++iter )
{
gnFileContig* fg = *iter;
*iter = 0;
delete fg;
}
}
boolean gnGBKSource::HasContig( const string& name ) const
{
for(uint32 i = 0 ; i <= m_contigList.size(); i++ )
{
if( name == m_contigList[i]->GetName() )
return true;
}
return false;
}
uint32 gnGBKSource::GetContigID( const string& name ) const
{
for(uint32 i = 0 ; i <= m_contigList.size(); i++ )
{
if( name == m_contigList[i]->GetName() )
return i;
}
return ALL_CONTIGS;
}
string gnGBKSource::GetContigName( const uint32 i ) const
{
if( i < m_contigList.size() )
{
return m_contigList[i]->GetName();
}
return "";
}
gnSeqI gnGBKSource::GetContigSeqLength( const uint32 i ) const
{
if( i == ALL_CONTIGS)
return m_spec->GetLength();
if( i < m_contigList.size() )
{
return m_contigList[i]->GetSeqLength();
}
return GNSEQI_ERROR;
}
boolean gnGBKSource::SeqRead( const gnSeqI start, char* buf, gnSeqI& bufLen, const uint32 contigI )
{
boolean result = false;
#pragma omp critical
{
result = SeqReadImpl( start, buf, bufLen, contigI );
}
return result;
}
boolean gnGBKSource::SeqReadImpl( const gnSeqI start, char* buf, gnSeqI& bufLen, const uint32 contigI ){
uint64 startPos = 0;
uint64 readableBytes = 0;
if( !SeqSeek( start, contigI, startPos, readableBytes ) )
{
bufLen = 0;
return false;
}
if( contigI == ALL_CONTIGS )
{
uint32 curLen = 0;
uint64 bytesRead = 0;
while (curLen < bufLen)
{
//SeqSeek to start, Figure out how much can be read before SeqSeeking again.
if(readableBytes <= 0) //Look out for zero length contigs! IMPLEMENT ME
if( !SeqSeek( start + curLen, contigI, startPos, readableBytes ) ){
bufLen = curLen;
return true;
}
//readLen is the amount to read on this pass
uint64 readLen = (bufLen - curLen) < readableBytes ? (bufLen - curLen) : readableBytes;
Array<gnSeqC> array_buf( readLen );
gnSeqC* tmpBuf = array_buf.data;
// read chars and filter
m_ifstream.read(tmpBuf, readLen);
uint64 gc = m_ifstream.gcount();
bytesRead += gc;
readableBytes -= gc;
for(uint32 i=0; i < gc; i++){
if( m_pFilter->IsValid(tmpBuf[i]) ){
buf[curLen] = tmpBuf[i];
curLen++;
}
}
if(m_ifstream.eof()){ //we hit the end of the file. bail out.
m_ifstream.clear();
bufLen = curLen;
return true;
}
}
bufLen = curLen;
}
else if( contigI < m_contigList.size() )
{
uint32 curLen = 0;
//check to see if the buffer is bigger than the contig. if so truncate it.
gnSeqI contigSize = m_contigList[contigI]->GetSeqLength();
bufLen = bufLen < contigSize ? bufLen : contigSize;
while (curLen < bufLen)
{
uint64 readLen = bufLen - curLen; //the amount to read on this pass
Array<gnSeqC> array_buf( readLen );
gnSeqC* tmpBuf = array_buf.data;
// read chars and filter
m_ifstream.read(tmpBuf, readLen);
uint64 gc = m_ifstream.gcount();
// cout << "Read " << gc << " chars from " << m_openString << "\n";
// cout << "Checking character validity on: " << tmpBuf << "\n";
for(uint32 i=0; i < gc; i++){
if( m_pFilter->IsValid(tmpBuf[i]) ){
buf[curLen] = tmpBuf[i];
curLen++;
}
}
if(m_ifstream.eof()){ //we hit the end of the file. bail out.
m_ifstream.clear();
bufLen = curLen;
return true;
}
}
bufLen = curLen;
}
return true;
}
// private:
// figures out which contig the sequence starts at then calls SeqStartPos to get the offset within that contig
// returns startPos, the file offset where the sequence starts
// returns true if successful, false otherwise
boolean gnGBKSource::SeqSeek( const gnSeqI start, const uint32& contigI, uint64& startPos, uint64& readableBytes )
{
if( contigI == ALL_CONTIGS )
{
// find first contig
gnSeqI curIndex = 0;
vector< gnFileContig* >::iterator iter = m_contigList.begin();
for( ; iter != m_contigList.end(); ++iter )
{
uint64 len = (*iter)->GetSeqLength();
if( (curIndex + len) > start )
break;
curIndex += len;
}
if( iter == m_contigList.end() )
return false;
// seek to start
gnSeqI startIndex = start - curIndex; //startIndex is starting pos. within the contig
return SeqStartPos( startIndex, *(*iter), startPos, readableBytes );
}
else if( contigI < m_contigList.size() )
{
return SeqStartPos( start, *(m_contigList[contigI]), startPos, readableBytes );
}
return false;
}
//Returns startPos, the file offset where the sequence starts.
boolean gnGBKSource::SeqStartPos( const gnSeqI start, gnFileContig& contig, uint64& startPos, uint64& readableBytes )
{
readableBytes = 0;
uint32 curLen = 0;
//seek to the file offset where the contig starts
startPos = contig.GetSectStartEnd(gnContigSequence).first; //set startPos to start where the contig starts
m_ifstream.seekg( startPos, ios::beg );
if( m_ifstream.eof() ){
ErrorMsg("ERROR in gnGBKSource::Incorrect contig start position, End of file reached!\n");
return false;
}
while( true )
{
// READ the rest of the contig skipping over invalid characters until we get to the starting base pair.
// startPos will contain the file offset with the starting base pair
uint32 tmpbufsize = contig.GetSectStartEnd(gnContigSequence).second - startPos;
if(tmpbufsize == 0){
ErrorMsg("ERROR in gnGBKSource: stored contig size is incorrect.");
return false;
}
uint64 startOffset = start;
if(contig.HasRepeatSeqGap()){ //check for sequence integrity
startOffset += (9 + m_newlineSize) * (start / 60 + 1) + start / 10 + 1;
if( m_newlineSize == 2 ) // test compensation for strange bug
startOffset--;
startPos+=startOffset;
m_ifstream.seekg(startPos , ios::beg);
readableBytes = contig.GetSectStartEnd(gnContigSequence).second - startPos;
return true;
}
//sequence is corrupt. read in base by base
tmpbufsize = tmpbufsize < BUFFER_SIZE ? tmpbufsize : BUFFER_SIZE; //read in the smaller of the two.
Array<char> array_buf( tmpbufsize );
char* tmpbuf = array_buf.data;
m_ifstream.read( tmpbuf, tmpbufsize );
if( m_ifstream.eof() ){
ErrorMsg("ERROR in gnGBKSource::Read End of file reached!\n");
return false;
}
for( uint32 i=0; i < tmpbufsize; ++i ){
if( m_pFilter->IsValid(tmpbuf[i]) ){
if( curLen >= start ){ //stop when we reach the starting offset within this contig
startPos += i;
m_ifstream.seekg( startPos, ios::beg ); //seek to startPos
readableBytes = contig.GetSectStartEnd(gnContigSequence).second - startPos;
return true;
}
++curLen; //each time we read a valid b.p., increment the sequence length
}
}
startPos += tmpbufsize;
}
return true;
}
void gnGBKSource::FormatString(string& data, uint32 offset, uint32 width){
//first remove newlines and corresponding whitespace
string::size_type newline_loc = data.find_first_of('\n', 0);
while(newline_loc != string::npos){
if(data[newline_loc-1] == '\r')
newline_loc--;
string::size_type text_loc = newline_loc;
while((data[text_loc] == ' ') ||(data[text_loc] == ' ')||(data[text_loc] == '\n')||(data[text_loc] == '\r')){
text_loc++;
if(text_loc+1 == data.length())
break;
}
data = (data.substr(0, newline_loc) + " " + data.substr(text_loc));
newline_loc = data.find_first_of('\n', 0);
}
//now reformat with newlines and whitespace, observing word boundaries...
string output_string = "";
for(uint32 charI = 0; charI < data.length();){
//get the substring to append and increment charI
string::size_type base_loc = charI;
string append_string;
while(base_loc - charI <= width){
string::size_type space_loc = data.find_first_of(' ', base_loc+1);
if(space_loc - charI < width)
base_loc = space_loc;
else if(base_loc == charI){
//word is too big for one line. split it.
append_string = data.substr(charI, width);
charI+=width;
}else{
append_string = data.substr(charI, base_loc - charI);
charI = base_loc;
}
}
output_string += string(offset, ' ') + append_string;
if(charI + width < data.length())
output_string += "\r\n";
}
data = output_string;
}
template< class SubSpec >
void WriteHeader(gnMultiSpec< SubSpec >* spec, const string& hdr, ofstream& m_ofstream) {
gnBaseHeader* gpbh = NULL;
uint32 header_index = 0;
try{
do{
gpbh = spec->GetHeader(hdr, header_index);
m_ofstream << gpbh->GetHeader();
header_index++;
}while(gpbh != NULL);
}catch(gnException& gne){}
}
boolean gnGBKSource::Write(gnSequence& seq, const string& filename){
ofstream m_ofstream(filename.c_str(), ios::out | ios::binary);
if(!m_ofstream.is_open())
return false;
string newline = "\r\n";
gnGenomeSpec* spec = seq.GetSpec();
// output general file header first if one exists.
if(spec->GetHeaderListLength() == 1){
gnBaseHeader *gpbh = spec->GetHeader(0);
string name = gpbh->GetHeaderName();
//IMPLEMENT ME! Is platform specific newline substitution necessary?
if(string::npos != name.find(".SEQ")){
string header = gpbh->GetHeader();
m_ofstream << header;
}
}
// TODO: Figure out where the buffer overflow is and reduce
// this back to BUFFER_SIZE -- overflow appears not to corrupt
// sequence data
Array<gnSeqC> array_buf( 2 * BUFFER_SIZE );
gnSeqC *bases = array_buf.data;
for(uint32 specI = 0; specI < spec->GetSpecListLength(); specI++){
gnFragmentSpec* subSpec = spec->GetSpec(specI);
//write out contig headers. start with LOCUS...
m_ofstream << "LOCUS ";
//write Locus Name
string contigName = subSpec->GetName();
if(contigName.length() > SEQ_LOCUS_NAME_LENGTH)
contigName = contigName.substr(0, SEQ_LOCUS_NAME_LENGTH);
uint32 filler_size = SEQ_LOCUS_NAME_LENGTH - contigName.length();
m_ofstream << contigName << string(filler_size, ' ');
//write Locus Length
string length_string = uintToString(subSpec->GetLength());
filler_size = SEQ_LOCUS_SIZE_LENGTH - length_string.size();
m_ofstream << string(filler_size, ' ') << length_string << " bp ";
//write dnatype
string dnatype = string(SEQ_LOCUS_DNATYPE_LENGTH, ' ');
uint32 head_look_i = 0;
gnBaseHeader* gpbh = NULL;
try{
gpbh = subSpec->GetHeader("LOCUS", head_look_i);
}catch(gnException& gne){}
if( gpbh != NULL )
dnatype = gpbh->GetHeader().substr(SEQ_LOCUS_DNATYPE_OFFSET, SEQ_LOCUS_DNATYPE_LENGTH);
m_ofstream << dnatype << string(2, ' ');
//write circularity
string circular = subSpec->IsCircular() ? string("circular ") : string(10, ' ');
m_ofstream << circular;
//write division code
string division = string(SEQ_LOCUS_DIVCODE_LENGTH, ' ');
if(gpbh != NULL)
division = gpbh->GetHeader().substr(SEQ_LOCUS_DIVCODE_OFFSET, SEQ_LOCUS_DIVCODE_LENGTH);
m_ofstream << division;
//write date -- IMPLEMENT ME! format the real date to spec! dd-mmm-yyyy
string date = string(SEQ_LOCUS_DATE_LENGTH, ' ');
if(gpbh != NULL)
date = gpbh->GetHeader().substr(SEQ_LOCUS_DATE_OFFSET, SEQ_LOCUS_DATE_LENGTH);
m_ofstream << string(7, ' ') << date << "\r\n";
//write out the rest of the headers if they were supplied!
WriteHeader(subSpec, "DEFINITION", m_ofstream);
WriteHeader(subSpec, "ACCESSION", m_ofstream);
WriteHeader(subSpec, "VERSION", m_ofstream);
WriteHeader(subSpec, "KEYWORDS", m_ofstream);
WriteHeader(subSpec, "SEGMENT", m_ofstream);
WriteHeader(subSpec, "SOURCE", m_ofstream);
WriteHeader(subSpec, "REFERENCE", m_ofstream);
WriteHeader(subSpec, "COMMENT", m_ofstream);
//write out feature table!
m_ofstream << "FEATURES Location/Qualifiers" << "\r\n";
for(uint32 featureI = 0; featureI < subSpec->GetFeatureListLength(); featureI++){
//write a feature tag
gnBaseFeature *gpmf = subSpec->GetFeature(featureI);
string featureName = gpmf->GetName();
m_ofstream << string(SEQ_SUBTAG_COLUMN, ' ') << featureName;
m_ofstream << string(SEQ_FEATURE_LOC_OFFSET - featureName.length() - SEQ_SUBTAG_COLUMN, ' ');
//ready to output location b.s.
uint32 location_count = gpmf->GetLocationListLength();
uint32 line_pos = SEQ_FEATURE_LOC_OFFSET;
uint32 parenthesis_count = 0;
if(location_count > 1){
m_ofstream << "join(";
line_pos += 5;
parenthesis_count++;
}
gnLocation::gnLocationType loc_type = gpmf->GetLocationType();
switch(loc_type){
case gnLocation::LT_Standard:
break;
case gnLocation::LT_Complement:
m_ofstream << "complement(";
line_pos += 11;
parenthesis_count++;
break;
case gnLocation::LT_Order:
m_ofstream << "order(";
line_pos += 6;
parenthesis_count++;
break;
case gnLocation::LT_Group:
m_ofstream << "group(";
parenthesis_count++;
line_pos += 6;
break;
case gnLocation::LT_OneOf:
m_ofstream << "one-of(";
parenthesis_count++;
line_pos += 7;
break;
default:
break;
}
//create the location string, then see if it will fit on the line
string location;
for(uint32 locationI = 0; locationI < location_count; locationI++){
gnLocation gpl = gpmf->GetLocation(locationI);
if(gpl.IsStartBoundLonger())
location += ">";
if(gpl.IsStartBoundShorter())
location += "<";
location += uintToString(gpl.GetStart());
gnSeqI end_loc = gpl.GetEnd();
if(end_loc != 0){
switch(gpl.GetType()){
case gnLocation::LT_BetweenBases:
location += "^";
break;
case gnLocation::LT_OneOf:
location += ".";
break;
default:
location += "..";
break;
}
if(gpl.IsEndBoundShorter())
location += "<";
if(gpl.IsEndBoundLonger())
location += ">";
location+= uintToString(end_loc);
}
if(locationI +1 < location_count)
location += ",";
else{ //append necessary parenthesis
for(;parenthesis_count > 0; parenthesis_count--)
location += ")";
}
//put it on this line if it fits. otherwise make a new line.
if(line_pos + location.length() < SEQ_COLUMN_WIDTH){
m_ofstream << location;
line_pos += location.length();
}else{
m_ofstream << "\r\n" << string(SEQ_FEATURE_LOC_OFFSET, ' ') << location;
line_pos = SEQ_FEATURE_LOC_OFFSET + location.length();
}
location = "";
}
m_ofstream << "\r\n";
//now output qualifiers! yaay!
//god damn this is a big ugly piece of code.
uint32 qualifier_count = gpmf->GetQualifierListLength();
for(uint32 qualifierI = 0; qualifierI < qualifier_count; qualifierI++){
m_ofstream << string(SEQ_FEATURE_LOC_OFFSET, ' ');
gnBaseQualifier* qualifier = gpmf->GetQualifier(qualifierI);
m_ofstream << "/" << qualifier->GetName() << "=";
//IMPLEMENT ME! do a better word wrap on this bitch.
string qually = string(qualifier->GetValue());
// FormatString(qually, SEQ_FEATURE_LOC_OFFSET, 80 - SEQ_FEATURE_LOC_OFFSET);
// qually = qually.substr(SEQ_FEATURE_LOC_OFFSET);
m_ofstream << qually << "\r\n";
}
if(gpmf != NULL)
delete gpmf;
}
//get information about the sequence we're writing out.
gnSeqI readOffset = seq.contigStart(specI);
gnSeqI readLength = seq.contigLength(specI);
//finally - output base count and origin
m_ofstream << "BASE COUNT ";
gnSeqI a_count=0, c_count=0, g_count=0, t_count=0, other_count=0;
gnSeqI countLen = readLength + readOffset;
for(gnSeqI countI = readOffset; countI < countLen;){
gnSeqI writeLen = countLen - countI < BUFFER_SIZE ? countLen - countI : BUFFER_SIZE;
if(!seq.ToArray(bases, writeLen, countI))
return false;
gnSeqI a, c, g, t, other;
BaseCount(string(bases, writeLen), a, c, g, t, other);
a_count += a;
c_count += c;
g_count += g;
t_count += t;
other_count += other;
countI += writeLen;
}
m_ofstream << uintToString(a_count) << " a ";
m_ofstream << uintToString(c_count) << " c ";
m_ofstream << uintToString(g_count) << " g ";
m_ofstream << uintToString(t_count) << " t ";
m_ofstream << uintToString(other_count) << " others" << "\r\n";
string origin = "ORIGIN\r\n";
head_look_i = 0;
try{
gpbh = subSpec->GetHeader("ORIGIN", head_look_i);
origin = gpbh->GetHeader();
m_ofstream << origin;
}catch(gnException& gne){
m_ofstream << "ORIGIN" << endl;
}
//write out the sequence
gnSeqI contig_bases = 0;
while(readLength > 0){ //buffer the read/writes
gnSeqI writeLen = readLength < BUFFER_SIZE + 20 ? readLength : BUFFER_SIZE + 20;
boolean success = seq.ToArray(bases, writeLen, readOffset);
if(!success)
return false;
//print each 60 on their own lines...
for(gnSeqI curbaseI = 0; curbaseI < writeLen; curbaseI += 60){
string baseIndexStr = uintToString(contig_bases + curbaseI +1);
m_ofstream << string(SEQ_BASES_INDEX_END - baseIndexStr.length(), ' ');
m_ofstream << baseIndexStr;
for(gnSeqI base_offset = 0; base_offset <= 50; base_offset+=10){
if(writeLen <= curbaseI + base_offset)
break;
int64 print_length = writeLen - (curbaseI + base_offset);
print_length = print_length > 10 ? 10 : print_length;
m_ofstream << ' ' << string(bases + curbaseI + base_offset, print_length);
}
m_ofstream << "\r\n";
}
readLength -= writeLen;
readOffset += writeLen;
contig_bases += writeLen;
}
m_ofstream << "//\r\n";
}
m_ofstream.close();
return true;
}
gnFileContig* gnGBKSource::GetFileContig( const uint32 contigI ) const{
if(m_contigList.size() > contigI)
return m_contigList[contigI];
return NULL;
}
//File parsing access routine
boolean gnGBKSource::ParseStream( istream& fin )
{
// INIT temp varables
uint32 readState = 0;
uint32 lineStart = 0;
// INIT buffer
uint32 sectionStart = 0;
uint64 streamPos = 0;
uint64 bufReadLen = 0;
uint64 remainingBuffer = 0;
Array<char> array_buf( BUFFER_SIZE );
char* buf = array_buf.data;
gnFragmentSpec* curFrag = 0;
gnSourceSpec* curSpec = 0;
gnSourceHeader *curHeader;
gnFeature* curFeature;
gnFileContig* curContig = 0;
gnLocation::gnLocationType curBaseLocationType;
gnSeqI curLocationStart;
int32 curStartLength = 0;
int32 curEndLength = 0;
string curLocContig = "";
string curQualifierName;
uint64 curQualifierStart;
string curContigName = "";
gnSeqI seqLength = 0;
gnSeqI seqChunk, seqChunkCount, gapChunk;
boolean corruptWarning = false;
//decide what type of newlines we have
DetermineNewlineType();
m_spec = new gnGenomeSpec();
while( !fin.eof() )
{
size_t newstart = sectionStart < lineStart ? sectionStart : lineStart;
if(sectionStart > 0){
if(readState == 14)
sectionStart = lineStart;
else if( sectionStart >= lineStart )
sectionStart -= lineStart;
remainingBuffer = bufReadLen - newstart;
memmove(buf, buf+newstart, remainingBuffer);
}
// read chars
fin.read( buf + remainingBuffer, BUFFER_SIZE - remainingBuffer);
streamPos -= remainingBuffer;
lineStart -= newstart;
bufReadLen = fin.gcount();
bufReadLen += remainingBuffer;
for( uint32 i=remainingBuffer ; i < bufReadLen ; i++ )
{
char ch = buf[i];
switch( readState )
{
case 0: //Assume we are in header at the start of a new line.
//Look for keywords starting in column 1
if((ch == '\n')&&(buf[lineStart] != ' ')&&(buf[lineStart] != '\t') && buf[lineStart] != '\r' && buf[lineStart] != '\n'){ //not equal to whitespace
if(curSpec == NULL){
curSpec = new gnSourceSpec(this, m_spec->GetSpecListLength());
curFrag = new gnFragmentSpec();
curFrag->AddSpec(curSpec);
curSpec->SetSourceName(m_openString);
m_spec->AddSpec(curFrag);
}
if(lineStart != sectionStart){ //Add the previous header to our list
uint32 j = SEQ_HEADER_NAME_LENGTH-1;
for(; j > 0; j--)
if((buf[sectionStart+j] != ' ')&&(buf[sectionStart+j] != ' '))
break;
string header_name = string(buf+sectionStart, j+1);
curHeader = new gnSourceHeader(this, header_name, sectionStart + streamPos, lineStart - sectionStart);
//if this is header info _before_ a locus statement then its a general file header.
if(strncmp(&buf[lineStart], "LOCUS", 5) == 0)
m_spec->AddHeader(curHeader);
else //otherwise its a fragment header.
curFrag->AddHeader(curHeader);
sectionStart = lineStart;
}
if(strncmp(&buf[lineStart], "FEATURES", 8) == 0){
sectionStart = i + 1;
readState = 1; //read in features
}else if(strncmp(&buf[lineStart], "ORIGIN", 6) == 0){
curHeader = new gnSourceHeader(this, string("ORIGIN"), sectionStart + streamPos, i - sectionStart + 1);
curFrag->AddHeader(curHeader);
curContig = new gnFileContig();
curContig->SetName(curContigName);
curContigName = "";
readState = 13; //read in base pairs
}else if(strncmp(&buf[lineStart], "LOCUS", 5) == 0){
if(strncmp(&buf[lineStart+SEQ_LOCUS_CIRCULAR_COLUMN-1], "circular", 8) == 0)
curFrag->SetCircular(true);
string locus_line = string(buf+lineStart, 80);
if( locus_line.find(" DNA ") == string::npos &&
locus_line.find(" dna ") == string::npos )
m_pFilter = gnFilter::proteinSeqFilter();
else
m_pFilter = gnFilter::fullDNASeqFilter();
uint32 j = SEQ_LOCUS_NAME_LENGTH+1;
for(; j > 0; j--)
if((buf[lineStart+SEQ_LOCUS_NAME_COLUMN+j-1] != ' ')&&(buf[sectionStart+SEQ_LOCUS_NAME_COLUMN+j-1] != ' '))
break;
curContigName = string(buf+lineStart+SEQ_LOCUS_NAME_COLUMN-1, j+1);
curFrag->SetName(curContigName);
}
}
if(ch == '\n'){
lineStart = i + 1;
}
break;
case 1: //look for feature tag in column six. ignore whitespace before feature.
if((ch == ' ')||(ch == ' ')){
break;
}else if(ch == '\n'){
lineStart = i + 1;
sectionStart = i + 1;
break;
}else if(sectionStart == i){ //there was no whitespace, we hit a TAG instead
i--;
readState = 0; //Deal with a Header TAG
sectionStart = i + 1;
break;
}else if((i - lineStart == SEQ_SUBTAG_COLUMN)||((buf[lineStart]==' ')&&(i==lineStart+1))){
sectionStart = i;
readState = 2;
} //
case 2: //Get the feature name. stop on whitespace
if((ch == ' ')||(ch == ' ')){
string featureName(buf+sectionStart, i - sectionStart);
curFeature = new gnFeature(featureName);
curFrag->AddFeature(curFeature);
sectionStart = i + 1;
readState = 3;
}
break;
case 3: //Ignore whitespace before feature location
if((ch == ' ')||(ch == ' ')){
break;
}else if((ch == '\r')||(ch == '\n')){
lineStart = i+1;
break;
}
sectionStart = i;
readState = 4;
// KNOWN BUG HERE!
// if JOIN is outside a group of complemented coordinates the feature type
// is incorrectly set to LT_COMPLEMENT. Instead each location's type should be set to LT_COMPLEMENT
case 4: //Read a location start. stop on (<.:^ and whitespace
if((ch == ' ')||(ch == ' ')||(ch == '(')||(ch == '.')||(ch=='^')||(ch==':')){
string starter(buf+sectionStart, i - sectionStart);
if(ch == '('){
if(starter == "complement")
curFeature->SetLocationType(gnLocation::LT_Complement);
else if(starter == "order")
curFeature->SetLocationType(gnLocation::LT_Order);
else if(starter == "group")
curFeature->SetLocationType(gnLocation::LT_Group);
else if(starter == "one-of")
curFeature->SetLocationType(gnLocation::LT_OneOf);
sectionStart = i + 1; //ignore join since it is default.
break;
}else if(ch == ':'){
curLocContig = starter;
sectionStart = i + 1;
break;
}
curLocationStart = atoi(starter.c_str());
readState = 6; //read in end base by default.
if(ch == '.'){
//go to special state to look for another one.
readState = 5;
sectionStart = i + 1;
break;
}else if(ch == '^'){
curBaseLocationType = gnLocation::LT_BetweenBases;
}else if((ch == ' ')||(ch == ' ')){
//no end location go to qualifier
gnLocation curLocation(curLocationStart, curLocationStart);
curFeature->AddLocation(curLocation, curFeature->GetLocationListLength());
readState = 7;
}
sectionStart = i + 1;
}else if(ch == '<'){
curStartLength = -1;
sectionStart = i + 1;
}else if(ch == '>'){
curStartLength = 1;
sectionStart = i + 1;
}
break;
case 5: //look for another period or location start.
if(ch == '.'){
curBaseLocationType = gnLocation::LT_Standard;
readState = 6;
sectionStart = i + 1;
break;
}
curBaseLocationType = gnLocation::LT_OneOf;
case 6: //see if there's a second location value. stop on >, and whitespace
if(ch == '>'){
curEndLength = 1;
sectionStart = i + 1;
}else if(ch == '<'){
curEndLength = -1;
sectionStart = i + 1;
}else if((ch == ' ')||(ch == ' ')||(ch == ',')||(ch == '\r')||(ch == '\n')){
//read end location
string ender(buf+sectionStart, i - sectionStart);
gnSeqI curLocationEnd = atoi(ender.c_str());
gnLocation curLocation(curLocationStart, curStartLength, curLocationEnd, curEndLength, curBaseLocationType);
curEndLength = 0;
curStartLength = 0;
curFeature->AddLocation(curLocation, curFeature->GetLocationListLength());
readState = ch == ',' ? 3 : 7; //read another loc if we need to.
sectionStart = i+1;
if( ch == '\n' )
lineStart = i+1;
}
break;
case 7: //skip to start of qualifier
if((ch != ' ')&&(ch != ' ')&&(lineStart == i)){
sectionStart = i; // Hit a tag. go deal with it.
readState = 0;
i--;
}else if((ch != ' ')&&(ch != ' ')&&((lineStart == i - SEQ_SUBTAG_COLUMN)||((buf[lineStart]==' ')&&(i==lineStart+1)))){
sectionStart = i; // Hit a feature. go deal with it.
readState = 2;
i--;
}else if(ch == ','){ //oops! another location to read!
sectionStart = i+1;
readState = 3;
}else if(ch == '/'){ //finally, a qualifier.
sectionStart = i+1;
readState = 8;
}else if(ch == '\n')
lineStart = i + 1;
break;
case 8: //get a qualifier, stop on =
if(ch == '='){
curQualifierName = string(buf+sectionStart, i - sectionStart);
readState = 9;
sectionStart = i+1;
}else if( ch == '\r' || ch == '\n' ){
// this is a value-less qualifier
curQualifierName = string(buf+sectionStart, i - sectionStart);
curFeature->AddQualifier( new gnStringQualifier( curQualifierName, "" ));
readState = 7;
sectionStart = i+1;
if( ch == '\n' )
lineStart = i + 1;
}
break;
case 9: //are we getting a string? look for " or [
if(ch == '"'){
readState = 10;
sectionStart = i;
curQualifierStart = i + streamPos;
}else if(ch == '['){
readState = 12;
sectionStart = i;
}else if((ch == '\r')||(ch == '\n')){
curFeature->AddQualifier(new gnSourceQualifier(this, curQualifierName, sectionStart + streamPos, i - sectionStart));
sectionStart = i+1;
readState = 7; //look for another qualifier
if( ch == '\n' )
lineStart = i + 1;
}
break;
case 10: //read until the end of the quotation. look out for escaped quotes
if(ch == '"')
readState = 11;
if(ch == '\n'){
lineStart = i + 1;
}
break;
case 11:
if(ch != '"'){
gnSourceQualifier* gnsq = new gnSourceQualifier(this, curQualifierName, curQualifierStart, i - sectionStart);
curFeature->AddQualifier(gnsq);
sectionStart = i+1;
readState = 7; //look for another qualifier.
if(ch == '\n')
lineStart = i + 1;
}else
readState = 10; //quote was escaped. look for another.
break;
case 12:
if(ch == ']'){
curFeature->AddQualifier(new gnSourceQualifier(this, curQualifierName, sectionStart + streamPos, i - sectionStart));
sectionStart = i+1;
readState = 7; //look for another qualifier.
}
break;
case 13: //start the sequence read.
curContig->SetSectStart(gnContigSequence, i - 1 + streamPos);
curContig->SetRepeatSeqGap(true);
seqChunk = 0;
seqChunkCount = 0;
gapChunk = m_newlineSize + 1;
readState = 14;
break;
case 14:
while(i < bufReadLen){
ch = buf[i];
if((ch == '/')&&(i==lineStart)){
readState = 15; //end of this sequence
break;
}else if(m_pFilter->IsValid(ch)){
if(gapChunk > 0){
if((gapChunk > 1 && seqChunkCount > 0) ||
(gapChunk != 10 + m_newlineSize && seqChunkCount == 0)){
if( !corruptWarning ){
ErrorMsg("File is corrupt. Proceed with caution.");
corruptWarning = true;
}
curContig->SetRepeatSeqGap(false);
}
gapChunk = 0;
}
seqChunk++;
seqLength++;
}else{
gapChunk++;
if(seqChunk == 10){
seqChunk = 0;
seqChunkCount++;
if(seqChunkCount == 6){
//got a complete line. start over
seqChunkCount = 0;
}
}
if(ch == '\n')
lineStart = i + 1;
}
i++;
}
break;
case 15:
if((ch == '\n')&&(buf[lineStart+1] == '/')){
curContig->SetSectEnd(gnContigSequence, lineStart - m_newlineSize + streamPos);
curContig->SetSeqLength(seqLength);
m_contigList.push_back(curContig);
curContig = 0;
curSpec->SetLength(seqLength);
curSpec = 0;
seqLength = 0;
lineStart = i + 1;
sectionStart = i + 1;
readState = 0;
}
break;
}
}
streamPos += bufReadLen;
}
if(curContig != 0){
curContig->SetSectEnd(gnContigSequence, streamPos - 1);
curContig->SetSeqLength(seqLength);
m_contigList.push_back(curContig);
curSpec->SetLength(seqLength);
}
if(curSpec != 0)
if((curFrag->GetFeatureListLength() == 0) && (curFrag->GetHeaderListLength() == 0)
&&(curSpec->GetLength() == 0)){
m_spec->RemoveSpec(m_spec->GetSpecListLength() - 1);
delete curFrag;
}
m_ifstream.clear();
return true;
}
} // end namespace genome
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