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/////////////////////////////////////////////////////////////////////////////
// File: gnSEQSource.h
// Purpose: Implements gnBaseSource for .SEQ files
// Description:
// Changes:
// Version: libGenome 0.5.1
// Author: Aaron Darling
// Modified by:
// Copyright: (c) Aaron Darling
// Licenses: See COPYING file for details
/////////////////////////////////////////////////////////////////////////////
#ifdef HAVE_CONFIG_H
#include "config.h"
#endif
#include "libGenome/gnFilter.h"
#include "libGenome/gnFeature.h"
#include "libGenome/gnSEQSource.h"
#include "libGenome/gnGBKSource.h"
#include "libGenome/gnSourceSpec.h"
#include "libGenome/gnSourceHeader.h"
#include "libGenome/gnSourceQualifier.h"
#include "libGenome/gnLocation.h"
#include "libGenome/gnStringTools.h"
#include "libGenome/gnDebug.h"
#include <cstring>
using namespace std;
namespace genome {
gnSEQSource::gnSEQSource()
{
m_openString = "";
m_pFilter = gnFilter::fullDNASeqFilter();
if(m_pFilter == NULL){
DebugMsg("Error using static sequence filters.\n");
}
}
gnSEQSource::gnSEQSource( const gnSEQSource& s ) : gnFileSource(s)
{
vector< gnFileContig* >::const_iterator iter = s.m_contigList.begin();
for( ; iter != s.m_contigList.end(); ++iter )
{
m_contigList.push_back( (*iter)->Clone() );
}
}
gnSEQSource::~gnSEQSource()
{
m_ifstream.close();
vector< gnFileContig* >::iterator iter = m_contigList.begin();
for( ; iter != m_contigList.end(); ++iter )
{
gnFileContig* fg = *iter;
*iter = 0;
delete fg;
}
}
boolean gnSEQSource::HasContig( const string& name ) const
{
for(uint32 i = 0 ; i <= m_contigList.size(); i++ )
{
if( name == m_contigList[i]->GetName() )
return true;
}
return false;
}
uint32 gnSEQSource::GetContigID( const string& name ) const
{
for(uint32 i = 0 ; i <= m_contigList.size(); i++ )
{
if( name == m_contigList[i]->GetName() )
return i;
}
return ALL_CONTIGS;
}
string gnSEQSource::GetContigName( const uint32 i ) const
{
if( i < m_contigList.size() )
{
return m_contigList[i]->GetName();
}
return "";
}
gnSeqI gnSEQSource::GetContigSeqLength( const uint32 i ) const
{
if( i == ALL_CONTIGS)
return m_spec->GetLength();
if( i < m_contigList.size() )
{
return m_contigList[i]->GetSeqLength();
}
return GNSEQI_ERROR;
}
boolean gnSEQSource::SeqRead( const gnSeqI start, char* buf, gnSeqI& bufLen, const uint32 contigI ){
uint64 startPos = 0;
uint64 readableBytes = 0;
if( !SeqSeek( start, contigI, startPos, readableBytes ) )
{
bufLen = 0;
return false;
}
if( contigI == ALL_CONTIGS )
{
uint32 curLen = 0;
uint64 bytesRead = 0;
while (curLen < bufLen)
{
//SeqSeek to start, Figure out how much can be read before SeqSeeking again.
if(readableBytes <= 0) //Look out for zero length contigs! IMPLEMENT ME
if( !SeqSeek( start + curLen, contigI, startPos, readableBytes ) ){
bufLen = curLen;
return true;
}
//readLen is the amount to read on this pass
uint64 readLen = (bufLen - curLen) < readableBytes ? (bufLen - curLen) : readableBytes;
gnSeqC* tmpBuf = new gnSeqC[readLen]; //read into tmpBuf, then filter tmpBuf into curBuf
// read chars and filter
m_ifstream.read(tmpBuf, readLen);
uint64 gc = m_ifstream.gcount();
bytesRead += gc;
readableBytes -= gc;
for(uint32 i=0; i < gc; i++){
if( m_pFilter->IsValid(tmpBuf[i]) ){
buf[curLen] = tmpBuf[i];
curLen++;
}
}
delete[] tmpBuf;
if(m_ifstream.eof()){ //we hit the end of the file. bail out.
m_ifstream.clear();
bufLen = curLen;
return true;
}
}
bufLen = curLen;
}
else if( contigI < m_contigList.size() )
{
uint32 curLen = 0;
//check to see if the buffer is bigger than the contig. if so truncate it.
gnSeqI contigSize = m_contigList[contigI]->GetSeqLength();
bufLen = bufLen < contigSize ? bufLen : contigSize;
while (curLen < bufLen)
{
uint64 readLen = bufLen - curLen; //the amount to read on this pass
gnSeqC* tmpBuf = new gnSeqC[readLen]; //read into tmpBuf, then filter tmpBuf into curBuf
// read chars and filter
m_ifstream.read(tmpBuf, readLen);
uint64 gc = m_ifstream.gcount();
// cout << "Read " << gc << " chars from " << m_openString << "\n";
// cout << "Checking character validity on: " << tmpBuf << "\n";
for(uint32 i=0; i < gc; i++){
if( m_pFilter->IsValid(tmpBuf[i]) ){
buf[curLen] = tmpBuf[i];
curLen++;
}
}
if(m_ifstream.eof()){ //we hit the end of the file. bail out.
m_ifstream.clear();
bufLen = curLen;
return true;
}
delete[] tmpBuf;
}
bufLen = curLen;
}
return true;
}
// private:
// figures out which contig the sequence starts at then calls SeqStartPos to get the offset within that contig
// returns startPos, the file offset where the sequence starts
// returns true if successful, false otherwise
boolean gnSEQSource::SeqSeek( const gnSeqI start, const uint32& contigI, uint64& startPos, uint64& readableBytes )
{
if( contigI == ALL_CONTIGS )
{
// find first contig
gnSeqI curIndex = 0;
vector< gnFileContig* >::iterator iter = m_contigList.begin();
for( ; iter != m_contigList.end(); ++iter )
{
uint64 len = (*iter)->GetSeqLength();
if( (curIndex + len) > start )
break;
curIndex += len;
}
if( iter == m_contigList.end() )
return false;
// seek to start
gnSeqI startIndex = start - curIndex; //startIndex is starting pos. within the contig
return SeqStartPos( startIndex, *(*iter), startPos, readableBytes );
}
else if( contigI < m_contigList.size() )
{
return SeqStartPos( start, *(m_contigList[contigI]), startPos, readableBytes );
}
return false;
}
//Returns startPos, the file offset where the sequence starts.
boolean gnSEQSource::SeqStartPos( const gnSeqI start, gnFileContig& contig, uint64& startPos, uint64& readableBytes )
{
readableBytes = 0;
uint32 curLen = 0;
//seek to the file offset where the contig starts
startPos = contig.GetSectStartEnd(gnContigSequence).first; //set startPos to start where the contig starts
m_ifstream.seekg( startPos, ios::beg );
if( m_ifstream.eof() ){
DebugMsg("ERROR in gnSEQSource::Incorrect contig start position, End of file reached!\n");
return false;
}
while( true )
{
// READ the rest of the contig skipping over invalid characters until we get to the starting base pair.
// startPos will contain the file offset with the starting base pair
uint32 tmpbufsize = contig.GetSectStartEnd(gnContigSequence).second - startPos;
if(tmpbufsize == 0){
DebugMsg("ERROR in gnSEQSource: stored contig size is incorrect.");
return false;
}
uint64 startOffset = start;
if(contig.HasRepeatSeqGap()){
if(contig.GetRepeatSeqGapSize().first > 0){
if(contig.GetRepeatSeqGapSize().second > 0){
startOffset += (start*contig.GetRepeatSeqGapSize().second)/contig.GetRepeatSeqGapSize().first;
startPos+=startOffset;
m_ifstream.seekg(startPos , ios::beg);
readableBytes = contig.GetSectStartEnd(gnContigSequence).second - startPos;
return true;
}
}else{
startPos+=start;
m_ifstream.seekg(startPos , ios::beg);
readableBytes = contig.GetSectStartEnd(gnContigSequence).second - startPos;
return true;
}
}
tmpbufsize = tmpbufsize < BUFFER_SIZE ? tmpbufsize : BUFFER_SIZE; //read in the smaller of the two.
char *tmpbuf = new char[tmpbufsize];
m_ifstream.read( tmpbuf, tmpbufsize );
if( m_ifstream.eof() ){
ErrorMsg("ERROR in gnSEQSource::Read End of file reached!\n");
delete[] tmpbuf;
return false;
}
for( uint32 i=0; i < tmpbufsize; ++i ){
if( m_pFilter->IsValid(tmpbuf[i]) ){
if( curLen >= start ){ //stop when we reach the starting offset within this contig
startPos += i;
m_ifstream.seekg( startPos, ios::beg ); //seek to startPos
readableBytes = contig.GetSectStartEnd(gnContigSequence).second - startPos;
delete[] tmpbuf;
return true;
}
++curLen; //each time we read a valid b.p., increment the sequence length
}
}
startPos += tmpbufsize;
delete[] tmpbuf;
}
return true;
}
//IMPLEMENT ME! move these static methods somewhere else! especially basecount!
void gnSEQSource::BaseCount(const string& bases, gnSeqI& a_count, gnSeqI& c_count, gnSeqI& g_count, gnSeqI& t_count, gnSeqI& other_count){
a_count = 0;
c_count = 0;
g_count = 0;
t_count = 0;
other_count = 0;
for(uint32 i=0; i < bases.length(); i++){
if((bases[i] == 'a')||(bases[i] == 'A'))
a_count++;
else if((bases[i] == 'c')||(bases[i] == 'C'))
c_count++;
else if((bases[i] == 'g')||(bases[i] == 'G'))
g_count++;
else if((bases[i] == 't')||(bases[i] == 'T'))
t_count++;
else
other_count++;
}
}
void gnSEQSource::FormatString(string& data, uint32 offset, uint32 width){
//first remove newlines and corresponding whitespace
string::size_type newline_loc = data.find_first_of('\n', 0);
while(newline_loc != string::npos){
if(data[newline_loc-1] == '\r')
newline_loc--;
string::size_type text_loc = newline_loc;
while((data[text_loc] == ' ') ||(data[text_loc] == ' ')||(data[text_loc] == '\n')||(data[text_loc] == '\r')){
text_loc++;
if(text_loc+1 == data.length())
break;
}
data = (data.substr(0, newline_loc) + " " + data.substr(text_loc));
newline_loc = data.find_first_of('\n', 0);
}
//now reformat with newlines and whitespace, observing word boundaries...
string output_string = "";
for(uint32 charI = 0; charI < data.length();){
//get the substring to append and increment charI
string::size_type base_loc = charI;
string append_string;
while(base_loc - charI <= width){
string::size_type space_loc = data.find_first_of(' ', base_loc+1);
if(space_loc - charI < width)
base_loc = space_loc;
else if(base_loc == charI){
//word is too big for one line. split it.
append_string = data.substr(charI, width);
charI+=width;
}else{
append_string = data.substr(charI, base_loc - charI);
charI = base_loc;
}
}
output_string += string(offset, ' ') + append_string;
if(charI + width < data.length())
output_string += "\r\n";
}
data = output_string;
}
boolean gnSEQSource::Write(gnGenomeSpec *spec, const string& filename){
ErrorMsg("Writing DNAStar SEQ files is not supported at this time. Try again next week.\n");
return false;
}
gnFileContig* gnSEQSource::GetFileContig( const uint32 contigI ) const{
if(m_contigList.size() > contigI)
return m_contigList[contigI];
return NULL;
}
//File parsing access routine
boolean gnSEQSource::ParseStream( istream& fin )
{
// INIT temp varables
uint32 readState = 0;
uint32 lineStart = 0;
int64 gapstart = -1;
// INIT buffer
uint32 sectionStart = 0;
uint64 streamPos = 0;
uint64 bufReadLen = 0;
uint64 remainingBuffer = 0;
char* buf = new char[BUFFER_SIZE];
gnFragmentSpec* curFrag = 0;
gnSourceSpec* curSpec = 0;
gnSourceHeader *curHeader;
gnBaseFeature* curFeature;
gnFileContig* curContig = 0;
gnLocation::gnLocationType curBaseLocationType;
gnSeqI curLocationStart;
int32 curStartLength = 0;
int32 curEndLength = 0;
string curLocContig = "";
string curQualifierName;
uint64 curQualifierStart;
string curContigName = "";
gnSeqI seqLength = 0;
gnSeqI lineSeqSize = 0;
m_spec = new gnGenomeSpec();
while( !fin.eof() )
{
if(sectionStart > 0){
if(readState == 15)
sectionStart = bufReadLen;
else if(readState == 16)
sectionStart = lineStart;
remainingBuffer = bufReadLen - sectionStart;
memmove(buf, buf+sectionStart, remainingBuffer);
}
// read chars
fin.read( buf + remainingBuffer, BUFFER_SIZE - remainingBuffer);
streamPos -= remainingBuffer;
lineStart -= sectionStart;
if(gapstart > 0)
gapstart -= sectionStart;
sectionStart = 0;
bufReadLen = fin.gcount();
bufReadLen += remainingBuffer;
for( uint32 i=remainingBuffer ; i < bufReadLen ; i++ )
{
char ch = buf[i];
switch( readState )
{
case 0: //Assume we are in header at the start of a new line.
//Look for keywords starting in column 1
if((ch == '\n')&&(buf[lineStart] != ' ')&&(buf[lineStart] != ' ')){ //not equal to space or tab
if(curSpec == NULL){
curSpec = new gnSourceSpec(this, m_spec->GetSpecListLength());
curFrag = new gnFragmentSpec();
curFrag->AddSpec(curSpec);
curSpec->SetSourceName(m_openString);
m_spec->AddSpec(curFrag);
}
if(lineStart != sectionStart){ //Add the previous header to our list
uint32 j = SEQ_HEADER_NAME_LENGTH-1;
for(; j > 0; j--)
if((buf[sectionStart+j] != ' ')&&(buf[sectionStart+j] != ' '))
break;
string header_name = string(buf+sectionStart, j+1);
curHeader = new gnSourceHeader(this, header_name, sectionStart + streamPos, lineStart - sectionStart);
//if this is header info _before_ a locus statement then its a general file header.
if(strncmp(&buf[lineStart], "LOCUS", 5) == 0)
m_spec->AddHeader(curHeader);
else //otherwise its a fragment header.
curFrag->AddHeader(curHeader);
sectionStart = lineStart;
}
if(strncmp(&buf[lineStart], "FEATURES", 8) == 0){
sectionStart = i + 1;
readState = 1; //read in features
}else if(strncmp(&buf[lineStart], "ORIGIN", 6) == 0){
curHeader = new gnSourceHeader(this, string("ORIGIN"), sectionStart + streamPos, i - sectionStart + 1);
curFrag->AddHeader(curHeader);
curContig = new gnFileContig();
curContig->SetName(curContigName);
curContigName = "";
readState = 13; //read in base pairs
}else if(strncmp(&buf[lineStart], "LOCUS", 5) == 0){
if(strncmp(&buf[lineStart+SEQ_LOCUS_CIRCULAR_COLUMN-1], "circular", 8) == 0)
curFrag->SetCircular(true);
uint32 j = SEQ_LOCUS_NAME_LENGTH + 1;
for(; j > 0; j--)
if((buf[lineStart+SEQ_LOCUS_NAME_COLUMN+j-1] != ' ')&&(buf[sectionStart+SEQ_LOCUS_NAME_COLUMN+j-1] != ' '))
break;
curContigName = string(buf+lineStart+SEQ_LOCUS_NAME_COLUMN-1, j+1);
curFrag->SetName(curContigName);
}else if(strncmp(&buf[lineStart], "^^", 2) == 0){
//start the sequence.
if(curContig == NULL){
curContig = new gnFileContig();
curContig->SetName(curContigName);
curContigName = "";
}
i--;
readState = 14;
break;
}
}
if(ch == '\n')
lineStart = i + 1;
break;
case 1: //look for feature tag in column six. ignore whitespace before feature.
if((ch == ' ')||(ch == ' ')){
break;
}else if(ch == '\n'){
lineStart = i + 1;
sectionStart = i + 1;
break;
}else if(sectionStart == i){ //there was no whitespace, we hit a TAG instead
i--;
readState = 0; //Deal with a Header TAG
sectionStart = i + 1;
break;
}else if((i - lineStart == SEQ_SUBTAG_COLUMN)||((buf[lineStart]==' ')&&(i==lineStart+1))){
sectionStart = i;
readState = 2;
} //
case 2: //Get the feature name. stop on whitespace
if((ch == ' ')||(ch == ' ')){
string featureName(buf+sectionStart, i - sectionStart);
curFeature = new gnFeature(featureName);
curFrag->AddFeature(curFeature);
sectionStart = i + 1;
readState = 3;
}
break;
case 3: //Ignore whitespace before feature location
if((ch == ' ')||(ch == ' ')){
break;
}else if((ch == '\r')||(ch == '\n')){
lineStart = i+1;
break;
}
sectionStart = i;
readState = 4;
case 4: //Read a location start. stop on (<.:^ and whitespace
if((ch == ' ')||(ch == ' ')||(ch == '(')||(ch == '.')||(ch=='^')||(ch==':')){
string starter(buf+sectionStart, i - sectionStart);
if(ch == '('){
if(starter == "complement")
curFeature->SetLocationType(gnLocation::LT_Complement);
else if(starter == "order")
curFeature->SetLocationType(gnLocation::LT_Order);
else if(starter == "group")
curFeature->SetLocationType(gnLocation::LT_Group);
else if(starter == "one-of")
curFeature->SetLocationType(gnLocation::LT_OneOf);
sectionStart = i + 1; //ignore join since it is default.
break;
}else if(ch == ':'){
curLocContig = starter;
sectionStart = i + 1;
break;
}
curLocationStart = atoi(starter.c_str());
readState = 6; //read in end base by default.
if(ch == '.'){
//go to special state to look for another one.
readState = 5;
sectionStart = i + 1;
break;
}else if(ch == '^'){
curBaseLocationType = gnLocation::LT_BetweenBases;
}else if((ch == ' ')||(ch == ' ')){
//no end location go to qualifier
gnLocation curLocation(curLocationStart, curLocationStart);
curFeature->AddLocation(curLocation, curFeature->GetLocationListLength());
readState = 7;
}
sectionStart = i + 1;
}else if(ch == '<'){
curStartLength = -1;
sectionStart = i + 1;
}else if(ch == '>'){
curStartLength = 1;
sectionStart = i + 1;
}
break;
case 5: //look for another period or location start.
if(ch == '.'){
curBaseLocationType = gnLocation::LT_Standard;
readState = 6;
sectionStart = i + 1;
break;
}
curBaseLocationType = gnLocation::LT_OneOf;
case 6: //see if there's a second location value. stop on >, and whitespace
if(ch == '>'){
curEndLength = 1;
sectionStart = i + 1;
}else if(ch == '<'){
curEndLength = -1;
sectionStart = i + 1;
}else if((ch == ' ')||(ch == ' ')||(ch == ',')){
//read end location
string ender(buf+sectionStart, i - sectionStart);
gnSeqI curLocationEnd = atoi(ender.c_str());
gnLocation curLocation(curLocationStart, curStartLength, curLocationEnd, curEndLength, curBaseLocationType);
curEndLength = 0;
curStartLength = 0;
curFeature->AddLocation(curLocation, curFeature->GetLocationListLength());
readState = ch == ',' ? 3 : 7; //read another loc if we need to.
sectionStart = i+1;
}
break;
case 7: //skip to start of qualifier
if((ch != ' ')&&(ch != ' ')&&(lineStart == i)){
sectionStart = i; // Hit a tag. go deal with it.
readState = 0;
i--;
}else if((ch != ' ')&&(ch != ' ')&&((lineStart == i - SEQ_SUBTAG_COLUMN)||((buf[lineStart]==' ')&&(i==lineStart+1)))){
sectionStart = i; // Hit a feature. go deal with it.
readState = 2;
i--;
}else if(ch == ','){ //oops! another location to read!
sectionStart = i+1;
readState = 3;
}else if(ch == '/'){ //finally, a qualifier.
sectionStart = i+1;
readState = 8;
}else if(ch == '\n')
lineStart = i + 1;
break;
case 8: //get a qualifier, stop on =
if(ch == '='){
curQualifierName = string(buf+sectionStart, i - sectionStart);
readState = 9;
sectionStart = i+1;
}
break;
case 9: //are we getting a string? look for " or [
if(ch == '"'){
readState = 10;
sectionStart = i;
curQualifierStart = i + streamPos;
}else if(ch == '['){
readState = 11;
sectionStart = i;
}else if((ch == '\r')||(ch == '\n')){
curFeature->AddQualifier(new gnSourceQualifier(this, curQualifierName, sectionStart + streamPos, i - sectionStart));
sectionStart = i+1;
readState = 7; //look for another qualifier
}
break;
case 10: //read until the end of the quotation. look out for escaped quotes
if(ch == '"')
readState = 11;
if(ch == '\n'){
lineStart = i + 1;
}
break;
case 11:
if(ch != '"'){
curFeature->AddQualifier(new gnSourceQualifier(this, curQualifierName, curQualifierStart, i - sectionStart));
sectionStart = i+1;
readState = 7; //look for another qualifier.
if(ch == '\n')
lineStart = i + 1;
}else
readState = 10; //quote was escaped. look for another.
break;
case 12:
if(ch == ']'){
curFeature->AddQualifier(new gnSourceQualifier(this, curQualifierName, sectionStart + streamPos, i - sectionStart));
sectionStart = i+1;
readState = 7; //look for another qualifier.
}
break;
case 13: //start the sequence read.
if(ch == '^') //stupid blattlab file format.
readState = 14;
else{
curContig->SetSectStart(gnContigSequence, i + 1 + streamPos);
readState = 16;
}
break;
case 14: //wait for newline before sequence starts.
if(ch == '\n'){
curContig->SetRepeatSeqGap(true);
lineStart = i + 1;
sectionStart = i + 1;
curContig->SetSectStart(gnContigSequence, i + 1 + streamPos);
readState = 15;
}
break;
case 15:
if(m_pFilter->IsValid(ch))
seqLength++;
else
curContig->SetRepeatSeqGap(false);
break;
case 16:
if((ch == '/')&&(i==lineStart)){
readState = 17;
}else if(m_pFilter->IsValid(ch)){
seqLength++;
lineSeqSize++;
if(gapstart >= 0){
curContig->SetRepeatGapSize(i - gapstart);
gapstart = -1;
}
}else if(ch == '\n'){ //IMPLEMENT ME! Needs consistent gap size checking
if(sectionStart == lineStart){
curContig->SetRepeatSeqGap(true);
curContig->SetRepeatSeqSize(seqLength);
gapstart = i;
for(; gapstart >= lineStart; gapstart--)
if(m_pFilter->IsValid(buf[gapstart]))
break;
gapstart++;
}else if(lineSeqSize != curContig->GetRepeatSeqGapSize().first)
curContig->SetRepeatSeqGap(false);
lineSeqSize = 0;
lineStart = i + 1;
}
break;
case 17:
if((ch == '\n')&&(buf[lineStart+1] == '/')){
curContig->SetSectEnd(gnContigSequence, lineStart - 2 + streamPos);
curContig->SetSeqLength(seqLength);
m_contigList.push_back(curContig);
curContig = 0;
curSpec->SetLength(seqLength);
curSpec = 0;
seqLength = 0;
lineStart = i + 1;
sectionStart = i + 1;
readState = 0;
}
break;
}
}
streamPos += bufReadLen;
}
if(curContig != 0){
curContig->SetSectEnd(gnContigSequence, streamPos - 1);
curContig->SetSeqLength(seqLength);
m_contigList.push_back(curContig);
curSpec->SetLength(seqLength);
}
if(curSpec != NULL)
if((curFrag->GetFeatureListLength() == 0) && (curFrag->GetHeaderListLength() == 0)
&&(curSpec->GetLength() == 0)){
m_spec->RemoveSpec(m_spec->GetSpecListLength() - 1);
delete curFrag;
}
m_ifstream.clear();
delete[] buf;
return true;
}
} // end namespace genome
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