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>CCGCCCTTGCCCTTCCTCCCTTCCCTTTCGGAGTCCTGGCCCCACCCTGT 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 0 0 perfect match
CCGCCCTTGCCCTTCCTCCCTTCCCTTTCGGAGTCCTGGCCCCACCCTGT 1..50 +synth1:31..80 start:0..end:0,matches:50,sub:0 segs:1,align_score:0,mapq:26
>ACAGGGTGGGGCCAGGACTCCGAAAGGGAAGGGAGGAAGGGCAAGGGCGG 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 1 1 perfect match on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAGGGAAGGGAGGAAGGGCAAGGGCGG 1..50 -synth1:80..31 start:0..end:0,matches:50,sub:0 segs:1,align_score:0,mapq:26
>CCGCCCTTGCCCTTCCTCCCTTCCCTCGTGGAGTCCTGGCCCCACCCTGT 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 2 2 mutation
CCGCCCTTGCCCTTCCTCCCTTCCCTttcGGAGTCCTGGCCCCACCCTGT 1..50 +synth1:31..80 start:0..end:0,matches:47,sub:3 segs:1,align_score:3,mapq:26
>ACAGGGTGGGGCCAGGACTCCGAAAGTAGAGGGAGGAAGGGCAAGGGCGG 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 3 3 mutation on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAGggaAGGGAGGAAGGGCAAGGGCGG 1..50 -synth1:80..31 start:0..end:0,matches:47,sub:3 segs:1,align_score:3,mapq:26
>CCGCCCTTGCCCTTCCTCCCTTCCCTATCTTCGGAGTCCTGGCCCCACCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 4 4 insertion
CCGCCCTTGCCCTTCCTCCCTTCCCT------------------------ 1..26 +synth1:31..56 start:0..ins:3,matches:26,sub:0 segs:2,align_score:2,mapq:26
,-----------------------------TTCGGAGTCCTGGCCCCACCC 30..50 +synth1:57..77 ins:3..end:0,matches:21,sub:0
>ACAGGGTGGGGCCAGGACTCCGAAAGATCGGAAGGGAGGAAGGGCAAGGG 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 5 5 insertion on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAG------------------------ 1..26 -synth1:80..55 start:0..ins:3,matches:26,sub:0 segs:2,align_score:2,mapq:26
,-----------------------------GGAAGGGAGGAAGGGCAAGGG 30..50 -synth1:54..34 ins:3..end:0,matches:21,sub:0
>CCGCCCTTGCCCTTCCTCCCTTCCCTGGAGTCCTGGCCCCACCCTGT 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456 6 6 deletion
CCGCCCTTGCCCTTCCTCCCTTCCCTttc--------------------- 1..26 +synth1:31..56 start:0..del:3,matches:26,sub:0 segs:2,align_score:2,mapq:26
,--------------------------GGAGTCCTGGCCCCACCCTGT 27..47 +synth1:60..80 del:3..end:0,matches:21,sub:0
>ACAGGGTGGGGCCAGGACTCCGAAAGAGGGAGGAAGGGCAAGGGCGG 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456 7 7 deletion on reverse strand
ACAGGGTGGGGCCAGGACTCCGAAAGgga--------------------- 1..26 -synth1:80..55 start:0..del:3,matches:26,sub:0 segs:2,align_score:2,mapq:26
,--------------------------AGGGAGGAAGGGCAAGGGCGG 27..47 -synth1:51..31 del:3..end:0,matches:21,sub:0
>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 8 8 perfect start of ref
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1..50 +synth1:1..50 start:0..end:0,matches:50,sub:0 segs:1,align_score:0,mapq:26
>GGGAGGAAGGGCAAGGGCGGCCGCGGGCACTGGGTCCAGCCCTGCCTCAG 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 9 9 perfect start of ref reverse strand
GGGAGGAAGGGCAAGGGCGGCCGCGGGCACTGGGTCCAGCCCTGCCTCAG 1..50 -synth1:50..1 start:0..end:0,matches:50,sub:0 segs:1,align_score:0,mapq:26
>GAGTCCTGGCCCCACCCTGTGCTTCCCCTCCGCCTGCTGCACAGGCGCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 10 10 perfect end of ref
GAGTCCTGGCCCCACCCTGTGCTTCCCCTCCGCCTGCTGCACAGGCGCCC 1..50 +synth1:61..110 start:0..end:0,matches:50,sub:0 segs:1,align_score:0,mapq:26
>GGGCGCCTGTGCAGCAGGCGGAGGGGAAGCACAGGGTGGGGCCAGGACTC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 11 11 perfect end of ref reverse strand
GGGCGCCTGTGCAGCAGGCGGAGGGGAAGCACAGGGTGGGGCCAGGACTC 1..50 -synth1:110..61 start:0..end:0,matches:50,sub:0 segs:1,align_score:0,mapq:26
>ATGAGGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 12 12 mismatch at beginning x1, starting at 1, with mutation at 20
cTGAGGCAGGGCTGGACCCaGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 2..50 +synth1:2..50 start:1..end:0,matches:48,sub:1 segs:1,align_score:2,mapq:26
>AAGAGGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 13 13 mismatch at beginning x2, starting at 1, with mutation at 20
ctGAGGCAGGGCTGGACCCaGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 3..50 +synth1:3..50 start:2..end:0,matches:47,sub:1 segs:1,align_score:3,mapq:26
>AAAAAGCAGGGCTGGACCCGGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 14 14 mismatch at beginning x5, starting at 1 (pos 4 actually does match), with mutation at 20
ctgAgGCAGGGCTGGACCCaGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 6..50 +synth1:6..50 start:5..end:0,matches:45,sub:1 segs:1,align_score:5,mapq:26
>CAGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 15 15 mismatch at beginning x1, starting at 2
CtGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 3..50 +synth1:3..50 start:2..end:0,matches:49,sub:0 segs:1,align_score:1,mapq:26
>CAAAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 16 16 mismatch at beginning x2, starting at 2
CtgAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 4..50 +synth1:4..50 start:3..end:0,matches:48,sub:0 segs:1,align_score:2,mapq:26
>CAAAAACAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 17 17 mismatch at beginning x5, starting at 2 (pos 4 actually does match)
CtgAggCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCC 7..50 +synth1:7..50 start:6..end:0,matches:46,sub:0 segs:1,align_score:4,mapq:26
>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCA 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 18 18 mismatch at end x1, starting at -1
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCCc 1..49 +synth1:1..49 start:0..end:1,matches:49,sub:0 segs:1,align_score:1,mapq:26
>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCAA 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 19 19 mismatch at end x2, starting at -1
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCcc 1..48 +synth1:1..48 start:0..end:2,matches:48,sub:0 segs:1,align_score:2,mapq:26
>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCAAAAA 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 20 20 mismatch at end x5, starting at -1
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCctccc 1..45 +synth1:1..45 start:0..end:5,matches:45,sub:0 segs:1,align_score:5,mapq:26
>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCAC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 21 21 mismatch at end x1, starting at -2
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTCcC 1..48 +synth1:1..48 start:0..end:2,matches:49,sub:0 segs:1,align_score:1,mapq:26
>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTAAC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 22 22 mismatch at end x2, starting at -2
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTCCTccC 1..47 +synth1:1..47 start:0..end:3,matches:48,sub:0 segs:1,align_score:2,mapq:26
>CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTAAAAAC 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 23 23 mismatch at end x5, starting at -2
CTGAGGCAGGGCTGGACCCAGTGCCCGCGGCCGCCCTTGCCCTTcctccC 1..44 +synth1:1..44 start:0..end:6,matches:45,sub:0 segs:1,align_score:5,mapq:26
>ACGCCCTTGCAATTCCTCCCTTCCCTTTCGGCCTGGCCCCACCCTGA 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456 24 24 padding left & right, mutation, deletion
cCGCCCTTGCccTTCCTCCCTTCCCTTTCGGagt---------------- 2..31 +synth1:32..61 start:1..del:3,matches:28,sub:2 segs:2,align_score:6,mapq:26
,-------------------------------CCTGGCCCCACCCTGt 32..46 +synth1:65..79 del:3..end:1,matches:15,sub:0
>ACGCCCTTGCAATTCCTCCCTTCCCTATCTTCGGAGTCCTGGCCCCACCA 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 25 25 padding left & right, mutation, insertion
cCGCCCTTGCccTTCCTCCCTTCCCT------------------------ 2..26 +synth1:32..56 start:1..ins:3,matches:23,sub:2 segs:2,align_score:6,mapq:26
,-----------------------------TTCGGAGTCCTGGCCCCACCc 30..49 +synth1:57..76 ins:3..end:1,matches:20,sub:0
>TGGTGGGGCCAGGACTCCGAAGATAGGGAAGGGAGGAATTGCAAGGGCGT 1 "#$%&'()*+,-./0123456789:;"#$%&'()*+,-./0123456789 26 26 padding left & right, mutation, insertion, reverse complemented
gGGTGGGGCCAGGACTCCGAA----------------------------- 2..21 -synth1:76..57 start:1..ins:3,matches:20,sub:0 segs:2,align_score:6,mapq:26
,------------------------AGGGAAGGGAGGAAggGCAAGGGCGg 25..49 -synth1:56..32 ins:3..end:1,matches:23,sub:2
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