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#!/bin/sh -x
# Determines the queue a job is submitted to
#PBS -q %PBS_QUEUE%
# Name of the job
#PBS -N %PBS_JOB_NAME%-aln
# Combine PBS error and output files.
#PBS -j oe
# Memory resource requirements
#PBS -l mem=%PBS_MEMORY%
# Mail job status at completion
#PBS -m ae
%PBS_MAILTO_DIRECTIVE%
%PBS_ARRAY_DIRECTIVE%
#
# Output some useful PBS job information
#
echo ------------------------------------------------------
echo Job is running on the following nodes:
cat ${PBS_NODEFILE}
echo ------------------------------------------------------
echo PBS: qsub is running on ${PBS_O_HOST}
echo PBS: originating queue is ${PBS_O_QUEUE}
echo PBS: executing queue is ${PBS_QUEUE}
echo PBS: working directory is ${PBS_O_WORKDIR}
echo PBS: execution mode is ${PBS_ENVIRONMENT}
echo PBS: job identifier is ${PBS_JOBID}
echo PBS: job name is ${PBS_JOBNAME}
echo PBS: task number is ${PBS_TASKNUM}
echo PBS: node number is ${PBS_NODENUM}
echo PBS: node file is ${PBS_NODEFILE}
echo PBS: array index is ${PBS_ARRAY_INDEX}
echo PBS: current home directory is ${PBS_O_HOME}
echo PBS: scratch directory is ${TMPDIR}
echo PBS: PATH = ${PBS_O_PATH}
echo ------------------------------------------------------
# The reference file to align with
REFERENCE=%REFERENCE%
REFERENCE_FILE=${REFERENCE##*/}
REFERENCE_INDEX_NAME=${REFERENCE_FILE%.*}.fasta
REFERENCE_INDEX_DIRECTORY=%REFERENCE_INDEX_DIRECTORY%
# The reads file to process
READS=%READS%
READS_FILE=${READS##*/}
# Copy the goby and support tools to the local node
# TODO: copy bwa and log4j, etc.
/bin/cp ${PBS_O_WORKDIR}/goby.jar ${TMPDIR}
# Extract the reads if a split is needed
if [ ! -z ${PBS_ARRAY_INDEX} ]; then
CHUNK_SIZE=%CHUNK_SIZE%
START_POSITION=$(($PBS_ARRAY_INDEX * $CHUNK_SIZE))
END_POSITION=$(($START_POSITION + $CHUNK_SIZE - 1))
cd ${TMPDIR}
java %PBS_JVM_FLAGS% -Dlog4j.debug=true -Dlog4j.configuration=file:log4j.properties \
-jar goby.jar --mode reformat-compact-reads --output ${READS_FILE} \
--start-position ${START_POSITION} --end-position ${END_POSITION} ${READS}
else
/bin/cp ${READS} ${TMPDIR}
fi
# Run the alignment
cd ${TMPDIR}
java %PBS_JVM_FLAGS% -Dlog4j.debug=true -Dlog4j.configuration=file:log4j.properties -jar goby.jar \
--mode align %COLORSPACE% --reference ${REFERENCE} --aligner %ALIGNER% --search \
--database-name ${REFERENCE_INDEX_NAME} --database-directory ${REFERENCE_INDEX_DIRECTORY} \
--reads ${READS_FILE} --basename %BASENAME%
# Copy the results back
if [ -z ${PBS_ARRAY_INDEX} ]; then
RESULT_DIR=${PBS_O_WORKDIR}/results/%BASENAME%
else
RESULT_DIR=${PBS_O_WORKDIR}/split-results/%BASENAME%-${PBS_ARRAY_INDEX}
fi
/bin/mkdir -p ${RESULT_DIR}
/bin/cp *.entries *.header *.stats *.tmh ${RESULT_DIR}
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