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#!/bin/env groovy
/*
* Copyright (C) 2009-2011 Institute for Computational Biomedicine,
* Weill Medical College of Cornell University
*
* This program is free software; you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation; either version 3 of the License, or
* (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
//
// After files have been sequenced by one or more Flow Cells
// (and de-multiplexed) and transferred from the facility that
// does the sequencing and converted to .compact-reads files,
// you are left with a directory structure similar to
// + FlowCellA
// + 001
// + s_1_sequence.compact-reads
// + ..
// + s_8_sequence.compact-reads
// + Summary.xml
// + 002
// + s_1_sequence.compact-reads
// + ..
// + s_8_sequence.compact-reads
// + Summary.xml
// + FlowCellB
// + 001
// + s_1_sequence.compact-reads
// + ..
// + s_8_sequence.compact-reads
// + Summary.xml
// + 002
// + s_1_sequence.compact-reads
// + ..
// + s_8_sequence.compact-reads
// + Summary.xml
//
// Combine this file structure with a tsv file which describes the
// data that has been sequenced, the --project-tsv file. This file
// The Summary.xml file converts from lane id (such as s_3_sequence.compact-reads
// comes from lane 3) to the sequencer-id (such as 32, which is generally something
// like S32 in the actual project). The --project-tsv file should have a column
// which is the sequencer-id, you specify which column this is with the
// --sample-id-column argument. Specify the columns you want to rename the file
// to with the --rename-columns argument. An example command for this script is,
// running in dry-run mode (no renames will take place):
//
// mkdir output-dir
// ./rename.groovy --dry-run -o ./output_dir -i sequencerId -p project-data.tsv
// -r shortSampleIdentifier typeOfSample phenotype _lane_ %lane _fc_ %flowCell
// -d FlowCell?/0*
//
// Run with --help to get complete usage information.
//
import org.apache.commons.cli.Option
class RenameSequencedFiles {
def FLOW_CELL_PATTERN = ~/Flow_*Cell_*([a-zA-Z]+)/
// The following PROCESS_SUFFIX should NOT be a regex
def PROCESS_SUFFIX = "compact-reads" // "txt.gz"
def READS_FILE_PATTERN = ~/^s_(\d+)_sequence\.${PROCESS_SUFFIX}$/
boolean dryRun
File outputFolderFile
File projectTsvFile
String sampleIdColumn
List<String> renameColumns
List<ProcessDetails> processDetailsList
Map<String, List<String>> sequencerIdToRenameColumns = [:]
// Special columns names are data that come from filenames or directory names
List<String> specialColumnNames = ["%flowCell", "%lane"]
public static void main(final String[] args) {
new RenameSequencedFiles().execute(args)
}
void execute(String[] args) {
// Configure command line args, parse --project-tsv, read Summary.xml files
if (!configure(args)) {
return
}
// Given the configuration, figure out the rename from's and to's
Map<String, String> renameFromToMap = createRenameMap()
if (!renameFromToMap) {
return
}
if (this.dryRun) {
// Dry run output
renameFromToMap.each { String k, String v ->
println "'${k}' would be renamed to '${v}'"
}
} else {
def renameEntries = renameFromToMap.entrySet()
// Verify the files to rename, source exists and dest doesn't exist
for (renameEntry in renameEntries) {
File source = new File(renameEntry.key)
File dest = new File(renameEntry.value)
if (!source.exists()) {
System.err.println "The file to rename FROM for ${source.toString()} to ${dest.toString()} doesn't exist."
return
} else if (dest.exists()) {
System.err.println "The file to rename TO for ${source.toString()} to ${dest.toString()} already exist."
return
}
}
// Perform the rename, then verify that it worked, the source file no longer exists and the
// dest file now does exist.
for (renameEntry in renameEntries) {
println "Moving/Renaming: '${renameEntry.key}' to '${renameEntry.value}'"
File source = new File(renameEntry.key)
File dest = new File(renameEntry.value)
def renamed = source.renameTo(dest)
if (!renamed) {
System.err.println "Move/Rename failed. It is likely you tried to move/rename across different filesystems."
return
}
if (source.exists()) {
System.err.println "The file to rename FROM exists after the rename, which it shouldn't."
return
} else if (!dest.exists()) {
System.err.println "The file to rename TO doesn't exist after the rename, which it should."
return
}
}
}
}
boolean configure(final String[] args) {
// Command line parsing configuration
def cli = new CliBuilder(usage: 'rename.groovy')
cli.h(longOpt: "help", "help")
cli.y(longOpt: "dry-run", "Execute 'dry-run' (don't actually rename / move files).")
cli.o(longOpt: "output-folder", args:1, required: true, "The output folder, which must be on the filesystem as the source files.")
cli.p(longOpt: "project-tsv", args:1, required: true, "The input tsv file that contains the project data.")
cli.i(longOpt: "sample-id-column", args:1, required: true,
"The name of the column that should exactly match the value " +
"from Summery/Samples/Lane/sampleId from the Summary.xml file.")
cli.r(longOpt: "rename-columns",
args: Option.UNLIMITED_VALUES, required: true, valueSeparator: ' ' as char,
"The names of the columns from the header line of the --project-tsv file, " +
"separated by spaces, used to construct the new filenames. " +
"String literals can be inlcuded by flanking the string with '_' and using '_' for " +
"spaces, such as '_cat_and_dog_' would be the literal 'cat and dog'. " +
"Special column names are ${specialColumnNames.join(', ')}. " +
"If you specify a single value @columns.txt, the columns can be read " +
"file the named file, one column per line.")
cli.d(longOpt: "process-dirs",
args: Option.UNLIMITED_VALUES, required: true, valueSeparator: ' ' as char,
"The directories to process, each specified directory should contain " +
"one or more .${PROCESS_SUFFIX} files, named in the " +
"format of s_#_sequence.${PROCESS_SUFFIX}, " +
"where # specifies the lane number, and a Summary.xml file. " +
"Each specified directory should contain the Flow Cell name, such as 'FlowCellA/006' so " +
"the script can determine from which Flow Cell the reads in each directory come. " +
"If you specify a single value @directories.txt, the directories can be read " +
"file the named file, one directory per line, but this method does not support wildcards.")
def options = cli.parse(args)
if (!options) {
// Options didn't parse
return false
}
if (options.h) {
cli.usage()
return false
}
// Retrieve pared command line options
this.dryRun = options.y
this.outputFolderFile = new File(options.o)
if (!this.outputFolderFile.exists() || !this.outputFolderFile.isDirectory()) {
System.err.println "Directory --output-folder doesn't exist or it isn't a directory or it isn't writable."
return false
}
this.projectTsvFile = new File(options.p)
this.sampleIdColumn = options.i
this.renameColumns = options.rs
this.processDetailsList = []
def directories = options.ds
// Verify the directories that were specified to be processed...
// that they are directories, they contain files, and each directory
// contains a Summary.xml file.
for (String directory in directories) {
File directoryFile = new File(directory)
if (!directoryFile.exists() || !directoryFile.isDirectory()) {
System.err.println "Cannot add directory ${directory} because it doesn't exist or isn't a directory."
return false
}
def flowCellName = flowCellFromDirName(directory)
if (!flowCellName) {
System.err.println "Could not find the flow cell specified for directory ${directoryFile.toString()}."
return false
}
File summaryFile = new File(directoryFile, "Summary.xml")
if (!summaryFile.exists() || !summaryFile.isFile()) {
System.err.println "Cannot find Summary.xml within ${directoryFile.toString()} because it doesn't exist or isn't a file."
return false
}
// Make sure the directory in question has some files that have the appropriate filenames
List<File> files = filesForDir(directoryFile)
if (!files) {
System.err.println "Cannot add directory ${directoryFile.toString()} because doesn't seem to contain any s_#_sequence.${PROCESS_SUFFIX} files."
return false
}
// Parse the XML summary file for a directory of files
Map<String, String> laneToSequencerIdMap = parseXmlSummaryFile(summaryFile)
if (!laneToSequencerIdMap) {
System.err.println "Error parsing Summary.xml file ${summaryFile.toString()}."
return false
}
processDetailsList << new ProcessDetails('flowCell': flowCellName, 'summaryFile': summaryFile, 'laneToSequencerIdMap': laneToSequencerIdMap, 'files': files)
}
if (!processDetailsList) {
System.err.println "No directories to process."
return false
}
// Make sure the output folder exists and is a directory
if (!this.outputFolderFile.exists() || !this.outputFolderFile.isDirectory()) {
System.err.println "The --output-folder ${this.outputFolderFile.toString()} directory does not exist."
return false
}
// Some sanity checks on the --project-tsv file exists and is a file
if (!this.projectTsvFile.exists() || !this.projectTsvFile.isFile()) {
System.err.println "The --project-tsv ${this.projectTsvFile.toString()} file did not exist or is not a file."
return false
}
if (!this.projectTsvFile.toString().endsWith(".tsv")) {
System.err.println "The --project-tsv ${this.projectTsvFile.toString()} filename didn't end with .tsv"
return false
}
// Read the project tsv file
if (!readProjectData()) {
return false
}
return true
}
boolean readProjectData() {
Map<String, Integer> columnNameToNumberMap = [:]
List<String> projectColumns
int lineNum = 1
Integer sampleIdColumnNum
for (String line in projectTsvFile.readLines()) {
if (line.startsWith("#")) {
continue
}
if (lineNum == 1) {
projectColumns = line.split("\t") as List
// Make a map of column name to column number
int colNo = 0
for (String projectColumn in projectColumns) {
if (!projectColumn) {
System.err.println "The --project-tsv file ${projectTsvFile.toString()} contains a column header that is blank."
return false
}
columnNameToNumberMap[projectColumn] = colNo++
}
// Find the sampleIdColumn
sampleIdColumnNum = columnNameToNumberMap[sampleIdColumn]
if (sampleIdColumnNum == null) {
System.err.println "The --sample-id-column ${sampleIdColumn} not found in --project-tsv ${this.projectTsvFile.toString()} file."
return false
}
// Verify that the rename columns are contained within projectColumns
for (String renameColumn in this.renameColumns) {
if (renameColumn.startsWith("_") && renameColumn.endsWith("_")) {
// Plain text, it's fine.
} else if (this.specialColumnNames.contains(renameColumn)) {
// Special column name it's fine
} else {
if (!projectColumns.contains(renameColumn)) {
System.err.println "The --rename-columns column ${renameColumn} not found in --project-tsv ${this.projectTsvFile.toString()} file."
return false
}
}
}
} else {
// Verify matching number of columns in data line
String[] lineParts = line.split("\t")
if (lineParts.size() != projectColumns.size()) {
System.err.println "The --project-tsv file ${projectTsvFile.toString()} line ${lineNum} should contain ${projectColumns.size()} columns but it contains ${lineParts.size()}."
return false
}
// Determine the rename values for one file
List<String> renameValues = []
for (String renameColumn in this.renameColumns) {
if (renameColumn.startsWith("_") && renameColumn.endsWith("_")) {
renameValues << renameColumn.replaceAll("_", " ").trim()
} else if (this.specialColumnNames.contains(renameColumn)) {
renameValues << renameColumn
} else {
renameValues << lineParts[columnNameToNumberMap[renameColumn]].trim()
}
}
sequencerIdToRenameColumns[lineParts[sampleIdColumnNum]] = renameValues
}
lineNum++
}
return true
}
/**
* Takes a String or a File and looks for the Flow Cell pattern, such that
* "FlowCellA/other" => "A"
* "stuffFlow_Cell__b/other" => "B"
* "stuff/Flow_CellBG/other" => "BG"
*/
String flowCellFromDirName(dir) {
def flowCellName = null
dir.toString().find(FLOW_CELL_PATTERN) { fullMatch, foundFlowCell ->
flowCellName = foundFlowCell.toUpperCase()
}
return flowCellName
}
/**
* Given a directory, find the files that match READS_FILE_PATTERN
*/
List<File> filesForDir(File dirFile) {
List<File> files = []
if (dirFile.exists()) {
dirFile.eachFileMatch(READS_FILE_PATTERN) { File match ->
files << match
}
}
return files
}
/**
* Given a file in the format s_6_sequence.compact-reads parse out the
* lane number (6, in this case).
*/
String laneFromFilename(File file) {
String filename = file.toString().replaceAll("\\\\","/").split("/")[-1]
String lane = null
filename.find(READS_FILE_PATTERN) { String fullmatch, String foundLane ->
lane = foundLane
}
return lane
}
/**
* Parse a map of sequencer-id to lane number from the
* Summary.xml file.
*/
Map<String, String> parseXmlSummaryFile(File summaryFile) {
Map<String, String> laneToSequencerIdMap = [:]
def xml = new XmlParser().parse(summaryFile)
def lanes = xml.Samples.Lane
for (lane in lanes) {
String laneNumber = lane.laneNumber.text().trim()
String sequencerId = lane.sampleId.text().trim()
laneToSequencerIdMap[laneNumber] = sequencerId
}
return laneToSequencerIdMap
}
/**
* Given the existing configuration and directories,
* create a rename from-to map.
*/
Map<String, String> createRenameMap() {
Map<String, String> renameFromToMap = [:]
for (ProcessDetails details in processDetailsList) {
String flowCell = details.flowCell
Map<String, String> laneToSequencerIdMap = details.laneToSequencerIdMap
File summaryFile = details.summaryFile
List<File> files = details.files
for (file in files) {
String lane = laneFromFilename(file)
String sequencerId = laneToSequencerIdMap[lane]
if (!sequencerId) {
System.err.println "Couldn't determine sequencer-id for lane ${lane} for file ${file} from Summary.xml file ${summaryFile}"
return null
}
List<String> renameValues = []
def renameColumns = sequencerIdToRenameColumns[sequencerId]
if (renameColumns != null) {
for (String renameColumn in sequencerIdToRenameColumns[sequencerId]) {
switch (renameColumn) {
case "%flowCell":
renameValues << flowCell
break
case "%lane":
renameValues << lane
break
default:
renameValues << renameColumn
break
}
}
String oldFilename = file.toString()
String newFilename = "${this.outputFolderFile}/${renameValues.join('-')}.${PROCESS_SUFFIX}"
if (renameFromToMap.containsKey(oldFilename) ||
renameFromToMap.containsValue(oldFilename) ||
renameFromToMap.containsKey(newFilename) ||
renameFromToMap.containsValue(newFilename)) {
System.err.println "Tried to add rename ${oldFilename} to ${newFilename} but one of the filenames was not unique."
return null
}
renameFromToMap[oldFilename] = newFilename
} else {
System.err.println "WARNING: sequencer-id ${sequencerId} not found in --project-tsv file ${projectTsvFile.toString()} so file ${file.toString()} cannot be renamed"
}
}
}
return renameFromToMap
}
}
class ProcessDetails {
String flowCell
File summaryFile
Map<String, String> laneToSequencerIdMap
List<File> files
}
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