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/*
* Copyright (C) 2009-2010 Institute for Computational Biomedicine,
* Weill Medical College of Cornell University
*
* This file is part of the Goby IO API.
*
* The Goby IO API is free software: you can redistribute it and/or modify
* it under the terms of the GNU Lesser General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* The Goby IO API is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU Lesser General Public License for more details.
*
* You should have received a copy of the GNU Lesser General Public License
* along with the Goby IO API. If not, see <http://www.gnu.org/licenses/>.
*/
package org.campagnelab.goby.alignments;
import org.campagnelab.goby.alignments.perms.NoOpPermutation;
import org.campagnelab.goby.alignments.perms.QueryIndexPermutation;
import org.campagnelab.goby.alignments.perms.QueryIndexPermutationInterface;
import org.campagnelab.goby.compression.MessageChunksWriter;
import org.campagnelab.goby.util.dynoptions.DynamicOptionClient;
import org.campagnelab.goby.util.dynoptions.RegisterThis;
import edu.cornell.med.icb.identifier.IndexedIdentifier;
import edu.cornell.med.icb.util.VersionUtils;
import it.unimi.dsi.fastutil.ints.IntArrayList;
import it.unimi.dsi.fastutil.ints.IntOpenHashSet;
import it.unimi.dsi.fastutil.ints.IntSet;
import it.unimi.dsi.fastutil.longs.LongArrayList;
import it.unimi.dsi.fastutil.objects.ObjectArrayList;
import it.unimi.dsi.fastutil.objects.ObjectList;
import it.unimi.dsi.lang.MutableString;
import org.apache.commons.io.FilenameUtils;
import org.apache.commons.io.IOUtils;
import org.apache.commons.logging.Log;
import org.apache.commons.logging.LogFactory;
import java.io.*;
import java.util.Map;
import java.util.Properties;
import java.util.zip.GZIPOutputStream;
/**
* This class write alignments in a Protocol Buffer, compressed, binary and split-able format.
* See Alignements.proto for the specification of this format.
*
* @author Fabien Campagne
* Date: Apr 30, 2009
* Time: 5:53:50 PM
*/
public class AlignmentWriterImpl implements AlignmentWriter {
/**
* Used to log debug and informational messages.
*/
private static final Log LOG = LogFactory.getLog(AlignmentWriterImpl.class);
private final Alignments.AlignmentCollection.Builder collectionBuilder;
private final MessageChunksWriter entriesChunkWriter;
private IndexedIdentifier queryIdentifiers;
private IndexedIdentifier targetIdentifiers;
private boolean headerWritten;
private final GZIPOutputStream headerOutput;
private boolean entriesHaveQueryLength;
private boolean entriesHaveQueryIndexOccurrences = true;
private boolean allReadQualityScores = true;
@RegisterThis
public static DynamicOptionClient doc = new DynamicOptionClient(AlignmentWriterImpl.class,
"permutate-query-indices:boolean, when true permutates query indices to small values (improves compression, but looses the ability to track alignments back to reads):false"
);
private ObjectArrayList<Alignments.ReadOriginInfo.Builder> readOriginInfoBuilderList;
private boolean entriesHaveAmbiguity = true;
public static DynamicOptionClient doc() {
return doc;
}
/**
* Details about aligner.
*/
private String alignerName;
private String alignerVersion;
/**
* The version of Goby that created this alignment file.
*/
private String gobyVersion;
/**
* Set of query lengths to determine whether or not they are unique.
*/
private final IntSet uniqueQueryLengths = new IntOpenHashSet();
/**
* Length of each target sequence.
*/
private int[] targetLengths;
private String[] queryIdentifiersArray;
private String[] targetIdentifiersArray;
private int maxTargetIndex = -1;
private int minQueryIndex = Integer.MAX_VALUE;
private int maxQueryIndex = Integer.MIN_VALUE;
private int actualNumberOfQueries = Integer.MIN_VALUE;
private final Properties stats;
private boolean statsWritten;
private final FileWriter statsWriter;
private final String basename;
private int numberOfAlignedReads;
private int constantQueryLength;
private boolean isConstantQueryLength;
private Alignments.AlignmentEntry.Builder newEntry;
private final FileOutputStream alignmentEntries;
private boolean sortedState;
// data structures to build index:
private int previousChunkOffset = -1;
private int firstTargetIndexInChunk;
private boolean firstEntryInChunk = true;
private int firstPositionInChunk;
private final LongArrayList indexOffsets = new LongArrayList();
private final LongArrayList indexAbsolutePositions = new LongArrayList();
private boolean indexWritten;
private long[] targetPositionOffsets;
private QueryIndexPermutationInterface permutator;
private boolean queryIndicesWerePermuted;
public AlignmentWriterImpl(final String outputBasename) throws IOException {
alignmentEntries = new FileOutputStream(outputBasename + ".entries");
headerOutput = new GZIPOutputStream(new FileOutputStream(outputBasename + ".header"));
statsWriter = new FileWriter(outputBasename + ".stats");
this.basename = outputBasename;
collectionBuilder = Alignments.AlignmentCollection.newBuilder();
entriesChunkWriter = new MessageChunksWriter(alignmentEntries);
entriesChunkWriter.setParser(new AlignmentCollectionHandler());
newEntry = Alignments.AlignmentEntry.newBuilder();
queryIdentifiers = new IndexedIdentifier();
targetIdentifiers = new IndexedIdentifier();
stats = new Properties();
// we assume stats were written until a client puts stats in this writer.
statsWritten = true;
setPermutation(doc.getBoolean("permutate-query-indices"));
}
/**
* Indicate whether query indices are small indices. When true, create a PermutationReader to reconstitute
* original query indices from the stored small indices.
*
* @param state True or False.
*/
@Override
public void setPermutation(boolean state) {
if (state) {
permutator = new QueryIndexPermutation(basename);
queryIndicesWerePermuted = true;
} else {
permutator = new NoOpPermutation();
queryIndicesWerePermuted = false;
}
}
@Override
public void setSorted(final boolean sortedState) {
this.sortedState = sortedState;
if (sortedState) {
if (targetPositionOffsets == null) {
final String s = "Indexing sorted alignments requires"
+ " knowning target lengths before entries are appended. setTargetLength"
+ " must be called before setSorted(true).";
LOG.error(s);
throw new UnsupportedOperationException(s);
}
} else {
// unsorted alignments are never permuted since the original query indices are already monotonically
// increasing in each chunk.
setPermutation(false);
}
}
/**
* Set the query index for the next alignment extry.
*
* @param queryIndex The query index of the next alignment entry to append
* @deprecated use
* {@link #appendEntry(Alignments.AlignmentEntry)}.
*/
@Deprecated
public final void setQueryIndex(final int queryIndex) {
newEntry.setQueryIndex(queryIndex);
}
/**
* Set the target index for the next alignment extry.
*
* @param targetIndex The target index of the next alignment entry to append
* @deprecated use
* {@link #appendEntry(Alignments.AlignmentEntry)}.
*/
@Deprecated
public final void setTargetIndex(final int targetIndex) {
newEntry.setTargetIndex(targetIndex);
}
/**
* Set the target position for the next alignment extry.
*
* @param position The target position of the next alignment entry to append
* @deprecated use
* {@link #appendEntry(Alignments.AlignmentEntry)}.
*/
@Deprecated
public final void setTargetPosition(final int position) {
newEntry.setPosition(position);
}
/**
* Set the score for the next alignment extry.
*
* @param score The score of the next alignment entry to append
* @deprecated use
* {@link #appendEntry(Alignments.AlignmentEntry)}.
*/
@Deprecated
public final void setAlignmentScore(final float score) {
newEntry.setScore(score);
}
public void setNumAlignmentEntriesPerChunk(final int numEntriesPerChunk) {
entriesChunkWriter.setNumEntriesPerChunk(numEntriesPerChunk);
}
/**
* Set fields for the next alignment extry.
*
* @param queryIndex The query index of the next alignment entry to append
* @param targetIndex The target index of the next alignment entry to append
* @param position The target position of the next alignment entry to append
* @param score The score of the next alignment entry to append
* @param matchesReverseStrand true if the entry matches the reverse strand
* @deprecated use
* {@link #appendEntry(Alignments.AlignmentEntry)}.
*/
@Deprecated
public final void setAlignmentEntry(final int queryIndex, final int targetIndex,
final int position,
final float score,
final boolean matchesReverseStrand, int queryLength) {
newEntry.setQueryIndex(queryIndex);
newEntry.setTargetIndex(targetIndex);
newEntry.setScore(score);
newEntry.setPosition(position);
newEntry.setMatchingReverseStrand(matchesReverseStrand);
newEntry.setMultiplicity(1);
newEntry.setQueryLength(queryLength);
}
/**
* Obtain the alignment entry that is being prepared. Set values on the entry,
* then call {@link #appendEntry()}.
*
* @return the current alignment entry.
* @deprecated use
* {@link #appendEntry(Alignments.AlignmentEntry)}.
*/
@Deprecated
public Alignments.AlignmentEntry.Builder getAlignmentEntry() {
return newEntry;
}
/**
* Append the current entry to the file being written.
*
* @throws IOException if the entry cannot be written
* @deprecated use
* {@link #appendEntry(Alignments.AlignmentEntry)}.
*/
@Deprecated
public synchronized void appendEntry() throws IOException {
// update the unique query length set
uniqueQueryLengths.add(newEntry.getQueryLength());
maxTargetIndex = Math.max(newEntry.getTargetIndex(), maxTargetIndex);
permutator.makeSmallIndices(newEntry);
if (newEntry.hasMultiplicity() && newEntry.getMultiplicity() == 1) {
newEntry.clearMultiplicity();
}
final Alignments.AlignmentEntry builtEntry = newEntry.build();
this.collectionBuilder.addAlignmentEntries(builtEntry);
writeIndexEntry(builtEntry);
newEntry = Alignments.AlignmentEntry.newBuilder();
}
private void writeIndexEntry(final Alignments.AlignmentEntryOrBuilder builtEntry) throws IOException {
// detect when all entries have query-index-occurrences:
entriesHaveQueryIndexOccurrences &= builtEntry.hasQueryIndexOccurrences();
// detect when all entries have ambiguity:
entriesHaveAmbiguity &= builtEntry.hasAmbiguity();
//detect when all entries have read_quality_scores:
allReadQualityScores &= builtEntry.hasReadQualityScores();
// detect when one or more entries have query length:
entriesHaveQueryLength |= builtEntry.hasQueryLength();
if (firstEntryInChunk) {
firstTargetIndexInChunk = builtEntry.getTargetIndex();
firstPositionInChunk = builtEntry.getPosition();
firstEntryInChunk = false;
}
final long currentChunkOffset = entriesChunkWriter.writeAsNeeded(collectionBuilder,
builtEntry.hasMultiplicity() ? builtEntry.getMultiplicity() : 1);
// LOG.warn(String.format("#entriesWritten: %d currentChunkOffset: %d previousChunkOffset: %d",
// entriesChunkWriter.getTotalEntriesWritten(), currentChunkOffset, previousChunkOffset));
if (sortedState && entriesChunkWriter.getAppendedInChunk() == 0) {
// we have just written a new chunk.
pushIndex(currentChunkOffset, firstTargetIndexInChunk, firstPositionInChunk);
firstEntryInChunk = true;
} else {
firstEntryInChunk = false;
}
}
private void pushIndex(final long startOfChunkOffset, final int firstTargetIndexInChunk, final int firstPositionInChunk) {
final long newOffset = Math.max(startOfChunkOffset, 0);
final int size = indexAbsolutePositions.size();
// remove duplicates because the behavior of binary search is undefined for duplicates:
/**
* Keep only the first absolutePosition we encounter and its offset in the file. This is done because if an
* absolute position repeats at the beginning of several consecutive chunks, we want to keep only the first.
* Also, binarySearch, which we use to access the indexAbsolutePositions array when the alignment is read
* has undefined behavior when duplicates exist in the array.
*/
// LOG.warn(String.format("INDEX attempting to push offset %d %d %n", firstTargetIndexInChunk, firstPositionInChunk));
final long codedPosition = recodePosition(firstTargetIndexInChunk, firstPositionInChunk);
if (size == 0 || codedPosition != indexAbsolutePositions.get(size - 1)) {
indexOffsets.add(newOffset);
indexAbsolutePositions.add(codedPosition);
if (LOG.isTraceEnabled()) {
LOG.trace(String.format("INDEX Pushing targetIndex= %d position= %d offset-in-file= %d absolutePosition= %d",
firstTargetIndexInChunk,
firstPositionInChunk,
newOffset, codedPosition));
}
}
}
protected long recodePosition(final int firstTargetIndexInChunk, final int firstPositionInChunk) {
assert firstTargetIndexInChunk < targetPositionOffsets.length : "Target length array must have enough elements to store each possible target index.";
return targetPositionOffsets[firstTargetIndexInChunk] + firstPositionInChunk;
}
/**
* Append an entry to the file being written. The entry must have been prepared
* outside this writer.
*
* @param entry The entry to append
* @throws IOException If an error occurs writing this entry.
*/
@Override
public synchronized void appendEntry(Alignments.AlignmentEntry entry) throws IOException {
if (entry.hasQueryLength()) {
// update the unique query length set
uniqueQueryLengths.add(entry.getQueryLength());
}
if (entry.hasMultiplicity() && entry.getMultiplicity() == 1) {
// we remove the multiplicity field, since there is no point in storing the default value:
newEntry = Alignments.AlignmentEntry.newBuilder(entry);
newEntry.clearMultiplicity();
entry = newEntry.build();
}
maxTargetIndex = Math.max(entry.getTargetIndex(), maxTargetIndex);
entry = permutator.makeSmallIndices(entry);
collectionBuilder.addAlignmentEntries(entry);
writeIndexEntry(entry);
numberOfAlignedReads += 1;
}
public boolean entriesHaveQueryIndexOccurrences() {
return entriesHaveQueryIndexOccurrences;
}
public boolean isAllReadQualityScores() {
return allReadQualityScores;
}
/**
* {@inheritDoc}
*/
@Override
public void close() throws IOException {
writeHeader();
writeStats();
permutator.close();
IOUtils.closeQuietly(headerOutput);
entriesChunkWriter.close(collectionBuilder);
if (sortedState && targetPositionOffsets != null) {
writeIndex();
}
IOUtils.closeQuietly(alignmentEntries);
IOUtils.closeQuietly(statsWriter);
}
private void writeIndex() throws IOException {
if (!indexWritten) {
// Push the last chunkoffset:
pushIndex(entriesChunkWriter.getCurrentChunkStartOffset(),
firstTargetIndexInChunk, firstPositionInChunk);
GZIPOutputStream indexOutput = null;
try {
indexOutput = new GZIPOutputStream(new FileOutputStream(basename + ".index"));
final Alignments.AlignmentIndex.Builder indexBuilder = Alignments.AlignmentIndex.newBuilder();
assert (indexOffsets.size() == indexAbsolutePositions.size()) : "index sizes must be consistent.";
indexBuilder.addAllOffsets(indexOffsets);
indexBuilder.addAllAbsolutePositions(indexAbsolutePositions);
indexBuilder.build().writeTo(indexOutput);
} finally {
if (indexOutput != null) indexOutput.close();
indexWritten = true;
}
}
}
private synchronized void writeHeader() throws IOException {
if (!headerWritten) {
final Alignments.AlignmentHeader.Builder headerBuilder = Alignments.AlignmentHeader.newBuilder();
// record the version of Goby that created this alignment..
final String version = VersionUtils.getImplementationVersion(AlignmentWriterImpl.class);
headerBuilder.setVersion(version);
headerBuilder.setLargestSplitQueryIndex(permutator.getBiggestSmallIndex());
headerBuilder.setSmallestSplitQueryIndex(permutator.getSmallestIndex());
headerBuilder.setNumberOfTargets(maxTargetIndex + 1);
headerBuilder.setNumberOfQueries(getNumQueries());
headerBuilder.setSorted(sortedState);
headerBuilder.setQueryIndicesWerePermuted(queryIndicesWerePermuted);
headerBuilder.setQueryIndexOccurrences(entriesHaveQueryIndexOccurrences);
headerBuilder.setAllReadQualityScores(allReadQualityScores);
// The Java implementation always indexes an index written in sorted order.
headerBuilder.setIndexed(sortedState);
headerBuilder.setQueryNameMapping(getMapping(queryIdentifiers, queryIdentifiersArray));
headerBuilder.setTargetNameMapping(getMapping(targetIdentifiers, targetIdentifiersArray));
headerBuilder.setNumberOfAlignedReads(numberOfAlignedReads);
if (alignerName != null) {
headerBuilder.setAlignerName(alignerName);
}
if (alignerVersion != null) {
headerBuilder.setAlignerVersion(alignerVersion);
}
// determine query lengths are constant (regardless of where they came from)
if (uniqueQueryLengths.size() == 1) {
// detected constant read length.
constantQueryLength = uniqueQueryLengths.iterator().nextInt();
headerBuilder.setConstantQueryLength(constantQueryLength);
isConstantQueryLength = true;
} else {
constantQueryLength = 0;
isConstantQueryLength = false;
}
if (readOriginInfoBuilderList != null) {
for (final Alignments.ReadOriginInfo.Builder builder : readOriginInfoBuilderList) {
headerBuilder.addReadOrigin(builder);
}
}
headerBuilder.setQueryLengthsStoredInEntries(true);
headerBuilder.setAmbiguityStoredInEntries(entriesHaveAmbiguity);
// store target lengths:
if (targetLengths != null) {
headerBuilder.addAllTargetLength(IntArrayList.wrap(targetLengths));
}
headerBuilder.setVersion(VersionUtils.getImplementationVersion(AlignmentWriterImpl.class));
headerBuilder.build().writeTo(headerOutput);
headerWritten = true;
}
}
private synchronized void writeStats() throws IOException {
if (!statsWritten) {
stats.put("basename", FilenameUtils.getName(basename));
stats.put("min.query.index", Integer.toString(permutator.getSmallestIndex()));
stats.put("max.query.index", Integer.toString(permutator.getBiggestSmallIndex()));
stats.put("number.of.queries", Integer.toString(getNumQueries()));
stats.put("basename.full", basename);
stats.put("number.alignment.entries", Integer.toString(numberOfAlignedReads));
stats.store(statsWriter, "Statistics for merged alignment. ");
statsWritten = true;
}
}
/**
* Provide query identifiers as an array of strings, where queryIndex is the index of the
* element in the array.
*
* @param queryIdentifiersArray Array of query identfiers
*/
@Override
public void setQueryIdentifiersArray(final String[] queryIdentifiersArray) {
this.queryIdentifiersArray = queryIdentifiersArray;
maxQueryIndex = queryIdentifiersArray.length - 1;
}
/**
* Provide target identifiers as an array of string, where targetIndex is the index of the
* element in the array.
*
* @param targetIdentifiersArray Array of target identfiers
*/
@Override
public void setTargetIdentifiersArray(final String[] targetIdentifiersArray) {
this.targetIdentifiersArray = targetIdentifiersArray;
maxTargetIndex = targetIdentifiersArray.length - 1;
}
private Alignments.IdentifierMapping getMapping(final IndexedIdentifier identifiers,
final String[] targetIdentifiersArray) {
final Alignments.IdentifierMapping.Builder result = Alignments.IdentifierMapping.newBuilder();
final ObjectList<Alignments.IdentifierInfo> mappings =
new ObjectArrayList<Alignments.IdentifierInfo>();
if (targetIdentifiersArray == null) {
for (final MutableString id : identifiers.keySet()) {
if (id != null) {
mappings.add(Alignments.IdentifierInfo.newBuilder()
.setName(id.toString())
.setIndex(identifiers.get(id))
.build());
}
}
} else {
// prefer to use the array when available, for speed:
for (int index = 0; index < targetIdentifiersArray.length; index++) {
if (targetIdentifiersArray[index] != null) {
mappings.add(Alignments.IdentifierInfo.newBuilder()
.setName(targetIdentifiersArray[index])
.setIndex(index)
.build());
}
}
}
result.addAllMappings(mappings);
return result.build();
}
public void printStats(final PrintStream out) {
this.entriesChunkWriter.printStats(out);
out.println("Min query index: " + permutator.getSmallestIndex());
out.println("Max query index: " + permutator.getBiggestSmallIndex());
out.println("Number of queries: " + getNumQueries());
out.println("Number of targets: " + (maxTargetIndex + 1));
}
@Override
public void setQueryIdentifiers(final IndexedIdentifier queryIdentifiers) {
this.queryIdentifiers = queryIdentifiers;
for (final int index : queryIdentifiers.values()) {
permutator.permutate(index, 2);
}
}
@Override
public void setTargetIdentifiers(final IndexedIdentifier targetIdentifiers) {
this.targetIdentifiers = targetIdentifiers;
for (final int index : targetIdentifiers.values()) {
maxTargetIndex = Math.max(maxTargetIndex, index);
}
}
@Override
public void setTargetLengths(final int[] targetLengths) {
assert targetLengths != null : "Target lengths cannot be null.";
assert targetLengths.length > maxTargetIndex
: "The number of elements of targetLength is too small to accommodate targetIndex="
+ maxTargetIndex;
// calculate the coding offset for each target index. This information will be used by recode
targetPositionOffsets = new long[targetLengths.length];
if (targetLengths.length > 0) {
targetPositionOffsets[0] = 0;
for (int targetIndex = 1; targetIndex < targetLengths.length; targetIndex++) {
targetPositionOffsets[targetIndex] =
targetLengths[targetIndex - 1] +
targetPositionOffsets[targetIndex - 1];
}
}
this.targetLengths = targetLengths;
}
/**
* Set the total number of queries.
*
* @param numQueries The number of query sequences.
*/
@Override
public void setNumQueries(final int numQueries) {
actualNumberOfQueries = numQueries;
maxQueryIndex = numQueries - 1;
}
/**
* Get the total number of queries.
*
* @return The number of query sequences.
*/
public int getNumQueries() {
if (actualNumberOfQueries != Integer.MIN_VALUE) {
return actualNumberOfQueries;
} else {
return permutator.getBiggestSmallIndex() - permutator.getSmallestIndex() + 1;
}
}
/**
* Set the total number of targets.
*
* @param numTargets The number of target sequences.
*/
@Override
public void setNumTargets(final int numTargets) {
maxTargetIndex = numTargets - 1;
}
@Override
public void putStatistic(final String description, final String value) {
statsWritten = false;
stats.put(description, value);
}
@Override
public void putStatistic(final String description, final double value) {
putStatistic(description, String.format("%3.3g", value));
}
@Override
public void putStatistic(final String description, final int value) {
putStatistic(description, String.format("%d", value));
}
public void setStatistics(final Properties statistics) {
for (final Map.Entry<Object, Object> statistic : statistics.entrySet()) {
putStatistic(statistic.getKey().toString(), statistic.getValue().toString());
}
}
public void setSmallestSplitQueryIndex(final int smallestQueryIndex) {
permutator.setSmallestIndex(smallestQueryIndex);
}
public void setLargestSplitQueryIndex(final int largestQueryIndex) {
permutator.setBiggestSmallIndex(largestQueryIndex);
}
public String getAlignerVersion() {
return alignerVersion;
}
@Override
public void setAlignerVersion(String alignerVersion) {
this.alignerVersion = alignerVersion;
}
public String getAlignerName() {
return alignerName;
}
@Override
public void setAlignerName(String alignerName) {
this.alignerName = alignerName;
}
@Override
public void setReadOriginInfo(ObjectArrayList<Alignments.ReadOriginInfo.Builder> readOriginInfoBuilderList) {
this.readOriginInfoBuilderList = readOriginInfoBuilderList;
}
@Override
public void addReadOriginInfo(ObjectArrayList<Alignments.ReadOriginInfo.Builder> readOriginInfoBuilderList) {
this.readOriginInfoBuilderList.addAll(readOriginInfoBuilderList);
}
}
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