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#ifndef SEQLIB_READ_FILTER_H
#define SEQLIB_READ_FILTER_H
#define AHO_CORASICK 1
#include <string>
#include <vector>
#include <climits>
#include "json/json.h"
#include "SeqLib/GenomicRegionCollection.h"
#include "SeqLib/BamRecord.h"
#ifdef HAVE_C11
#include "SeqLib/aho_corasick.hpp"
#endif
#define MINIRULES_MATE_LINKED 1
#define MINIRULES_MATE_LINKED_EXCLUDE 2
#define MINIRULES_REGION 3
#define MINIRULES_REGION_EXCLUDE 4
namespace SeqLib {
typedef SeqHashSet<std::string> StringSet;
namespace Filter {
#ifdef HAVE_C11
/** Tool for using the Aho-Corasick method for substring queries of
* using large dictionaries
* @note Trie construction / searching implemented by https://github.com/blockchaindev/aho_corasick
*/
struct AhoCorasick {
/** Allocate a new empty trie */
AhoCorasick() {
aho_trie = SeqPointer<aho_corasick::trie>(new aho_corasick::trie());
inv = false;
count = 0;
}
/** Deallocate the trie */
~AhoCorasick() { }
/** Add a motif to the trie
* @note Trie construction is lazy. Won't build trie until
* first query. Therefore first query is slow, the rest are
* O(n) where (n) is length of query string.
*/
void AddMotif(const std::string& m) {
aho_trie->insert(m);
}
/** Add a set of motifs to the trie from a file
* @param f File storing the motifs (new line separated)
* @exception Throws a runtime_error if file cannot be opened
*/
void TrieFromFile(const std::string& f);
/** Query if a string is in the trie
* @param t Text to query
* @return Returns number of substrings in tree that are in t
*/
int QueryText(const std::string& t) const;
SeqPointer<aho_corasick::trie> aho_trie; ///< The trie for the Aho-Corasick search
std::string file; ///< Name of the file holding the motifs
bool inv; ///< Is this an inverted dictinary (ie exclude hits)
int count; ///< Number of motifs in dictionary
};
#endif
/** Stores a rule for a single alignment flag.
*
* Rules for alignment flags can be one of three states:
* - NA - All flag values are valid
* - Off - Flag is valid if OFF
* - On - Flag is valid if ON
*/
class Flag {
public:
/** Construct a new Flag with NA rule
*/
Flag() : on(false), off(false), na(true) {}
/** Set the flag to NA (pass alignment regardless of flag value) */
void setNA() { on = false; off = false; na = true; }
/** Set the flag to ON (require flag ON to pass) */
void setOn() { on = true; off = false; na = false; }
/** Set the flag to OFF (require flag OFF to pass) */
void setOff() { on = false; off = true; na = false; }
/** Return if the Flag filter is NA */
bool isNA() const { return na; }
/** Return if the Flag filter is ON */
bool isOn() const { return on; }
/** Return if the Flag filter is OFF */
bool isOff() const { return off; }
/** Parse the Flag rule from a JSON entry */
bool parseJson(const Json::Value& value, const std::string& name);
private:
bool on;
bool off;
bool na;
};
/** Filter numeric values on whether they fall in/out of a range of values (eg mapping quality).
*
* Can optionally invert the range to make rule the complement of the range
* (eg insert-size NOT in [300,600]
*/
class Range {
public:
/** Construct a default range with everything accepted
*/
Range() : m_min(0), m_max(0), m_inverted(false), m_every(true) {}
/** Construct a Range from min to max, inclusive
* @param min Minimum for range
* @param max Maximum for range
* @param inverted Declare if this should be an inverted range (do NOT accept vals in range)
*/
Range(int min, int max, bool inverted) : m_min(min), m_max(max), m_inverted(inverted), m_every(false) {}
/** Given a query value, determine if the value passes this Range
* @param val Query value (e.g. mapping quality)
* @return true if the value passes this Range rule
*/
bool isValid(int val) {
if (m_every)
return true;
if (!m_inverted)
return (val >= m_min && val <= m_max);
else
return (val < m_min || val > m_max);
}
/** Parse a JSON value
* @param value
* @param name
*/
void parseJson(const Json::Value& value, const std::string& name);
/** Print the contents of this Range */
friend std::ostream& operator<<(std::ostream &out, const Range &r);
/** Return if this range accepts all values */
bool isEvery() const { return m_every; }
/** Return the lower bound of the range */
int lowerBound() const { return m_min; }
/** Return the upper bound of the range */
int upperBound() const { return m_max; }
/** Return true if the range is inverted (e.g. do NOT accept i in [min,max] */
bool isInverted() const { return m_inverted; }
private:
int m_min;
int m_max;
bool m_inverted;
bool m_every;
};
/** Stores a set of Flag objects for filtering alignment flags
*
* An alignment can be queried against a FlagRule to check if it
* satisfies the requirements for its alignment flag.
*/
class FlagRule {
public:
FlagRule() {
dup = Flag();
supp = Flag();
qcfail = Flag();
hardclip = Flag();
fwd_strand = Flag();
rev_strand = Flag();
mate_fwd_strand = Flag();
mate_rev_strand = Flag();
mapped = Flag();
mate_mapped = Flag();
ff = Flag();
fr = Flag();
rf = Flag();
rr = Flag();
ic = Flag();
paired = Flag();
m_all_on_flag = 0;
m_all_off_flag = 0;
m_any_on_flag = 0;
m_any_off_flag = 0;
every = false;
}
Flag dup; ///< Filter for duplicated flag
Flag supp; ///< Flag for supplementary flag
Flag qcfail; ///< Flag for qcfail flag
Flag hardclip; ///< Flag for presence of hardclip in cigar string
Flag fwd_strand; ///< Flag for forward strand alignment
Flag rev_strand; ///< Flag for reverse strand alignment
Flag mate_fwd_strand; ///< Flag for forward strand alignment for mate
Flag mate_rev_strand; ///< Flag for reverse strand alignment for mate
Flag mapped; ///< Flag for mapped alignment
Flag mate_mapped; ///< Flag for mate-mapped alignment
Flag ff; ///< Flag for both reads on forward strand
Flag fr; ///< Flag for lower (by position) read on forward strand, higher on reverse
Flag rf; ///< Flag for lower (by position) read on reverse strand, higher on forward
Flag rr; ///< Flag for both reads on reverse strand
Flag ic; ///< Flag for read and mate aligned to different chromosomes
Flag paired; ///< Flag for read is part of pair
/** Parse a FlagRule from a JSON object
*/
void parseJson(const Json::Value& value);
/** Set rule to pass all alignment flags that have any bit in f on
* @param f Alignment flags to be on for alignment record to pass
*/
void setAnyOnFlag(uint32_t f) { m_any_on_flag = f; every = (every && f == 0); }
// NOTE: every = (every && f == 0) means to set every to true only if
// input flag is zero and every was already true
/** Set rule to pass all alignment flags that have any bit in f off
* @param f Alignment flags to be off for alignment record to pass
*/
void setAnyOffFlag(uint32_t f) { m_any_off_flag = f; every = (every && f == 0); }
/** Set rule to reject all alignment flags that have any bit in f off
* @param f Alignment flags to be on for alignment record to pass
*/
void setAllOnFlag(uint32_t f) { m_all_on_flag = f; every = (every && f == 0); }
/** Set rule to reject all alignment flags that have any bit in f on
* @param f Alignment flags to be off for alignment record to pass
*/
void setAllOffFlag(uint32_t f) { m_all_off_flag = f; every = (every && f == 0); }
/** Return whether a read passes the alignment flag rules in this object
* @param r Alignment record to query
* @return true if record passes rules
*/
bool isValid(const BamRecord &r);
/** Print the flag rule */
friend std::ostream& operator<<(std::ostream &out, const FlagRule &fr);
/** Return if every this object will pass all records provided to it */
bool isEvery() const { return every; }
private:
bool every; // does this pass all flags?
uint32_t m_all_on_flag; // if read has all of these, keep
uint32_t m_all_off_flag; // if read has all of these, fail
uint32_t m_any_on_flag; // if read has any of these, keep
uint32_t m_any_off_flag;// if read has any of these, fail
int parse_json_int(const Json::Value& v);
};
/** Stores a full rule (Flag + Range + motif etc)
*
* An alignment can be queried with an AbstractRule object
* to check if it passes that rule.
*/
class AbstractRule {
friend class ReadFilter;
friend class ReadFilterCollection;
public:
/** Create empty rule with default to accept all */
AbstractRule() : m_count(0), subsam_frac(1), subsam_seed(999) { }
/** Destroy the filter */
~AbstractRule() {}
/** Add a list of motifs that will be search as sub-strings
* of the read sequence
* @param f Path to new-line separted file of motifs
* @param inverted If true, the reads that have a matching motif will fail isValid
*/
void addMotifRule(const std::string& f, bool inverted);
/** Query a read against this rule. If the
* read passes this rule, return true.
* @param r An aligned sequencing read to query against filter
*/
bool isValid(const BamRecord &r);
/** Supply the rule parameters with a JSON
* @param value JSON object created by parsing a string
*/
void parseJson(const Json::Value& value);
/** Print some basic information about this filter
*/
friend std::ostream& operator<<(std::ostream &out, const AbstractRule &fr);
/** Return if this rule accepts all reads
*/
bool isEvery() const;
/** Set the rate to subsample (default 1 = no subsampling)
* @param s A rate between 0 and 1
*/
void SetSubsampleRate(double s) { subsam_frac = s; };
/** Supply a name for this rule
* @param s ID to be associated with this rule
*/
void SetRuleID(const std::string& s) { id = s; };
/** Specify a read-group for this filter.
* Reads that do not belong to this read group
* will not pass isValid
* @param rg Read group to be matched against RG:Z:readgroup
*/
void SetReadGroup(const std::string& rg) { read_group = rg; }
FlagRule fr; ///< FlagRule specifying the alignment flag filter
Range isize; ///< Range object for insert-size filter
Range mapq; ///< Range object for mapping quality filter
Range len; ///< Range object for length filter
Range phred; ///< Range object for base-quality filter
Range clip; ///< Range object for number of clipped bases filter
Range nm; ///< Range object for NM (num mismatch) filter
Range nbases; ///< Range object for number of "N" bases filer
Range ins; ///< Range object for max CIGAR insertion size filter
Range del; ///< Range object for max CIGAR deletion size filter
Range xp; ///< Range object for number of secondary alignments
private:
void parseSeqLine(const Json::Value& value);
// read group
std::string read_group;
// how many reads pass this rule?
size_t m_count;
// the aho-corasick trie
#ifdef HAVE_C11
AhoCorasick aho;
#endif
// id for this rule
std::string id;
// fraction reads to subsample
double subsam_frac;
// data
uint32_t subsam_seed; // random seed for subsampling
void parseSubLine(const Json::Value& value);
};
class ReadFilterCollection;
/**
* A set of AbstractRules on a region united by logi rules.
*
* ReadFilter stores an arbitrarily complex collection of AbstractRules
* (e.g. (Mapped && Clipped) || (Unmapped)).
*/
class ReadFilter {
friend class ReadFilterCollection;
public:
/** Construct an empty filter that passes all reads */
ReadFilter() : excluder(false), m_applies_to_mate(false), m_count(0) {}
/** Destroy the filter */
~ReadFilter();
// ReadFilter(const ReadFilter& rf);
// Make a ReadFilter with an all exclude or include rule
// @param Samtools style string, BED file or VCF
// @param reg_type The type of rule this will be
// @param h BAM header that defines available chromosomes
///
//ReadFilter(const CommandLineRegion& c, const BamHeader& hdr);
/** Return whether a read passes this filter
* @param r A read to query
* @note If this is an excluder rule, then this
* returns false if the read passes the filter
*/
bool isValid(const BamRecord &r);
/** Add a rule to this filter. A read must pass all
* of the rules contained in this filter to pass
* @param ar A rule (eg MAPQ > 30) that the read must satisfy to pass this filter.
*/
void AddRule(const AbstractRule& ar);
/** Provide the region covered by this read filter
* @param g Region that this filter applies to
*/
void setRegions(const GRC& g);
/** Add additional regions to the filtered region
* @param g Additional regions to be included in filter
*/
void addRegions(const GRC& g);
/** Check if a read is overlapping the region defined by this filter
* @param r Read to query whether it overlaps (even partially) the region.
* @note If this is a mate-linked region, then the read will overlap
* if its mate overlaps as well.
*/
bool isReadOverlappingRegion(const BamRecord &r) const;
/** Print basic information about this filter */
friend std::ostream& operator<<(std::ostream& out, const ReadFilter &mr);
/** Return the number of rules in this filter */
size_t size() const {
return m_abstract_rules.size();
}
/** Set as an excluder region
* An excluder region is such that if a read satisfies
* this rule, then it will fail isValid, rather than pass
*/
void SetExcluder(bool e) { excluder = e; }
/** Set as a mate linked region */
void SetMateLinked(bool e) { m_applies_to_mate = e; }
private:
GRC m_grv; // the interval tree with the regions this rule applies to. Empty is whole-genome
std::string id; // set a unique id for this filter
bool excluder; // this filter is such that if read passes, it gets excluded
std::string m_region_file;
std::vector<AbstractRule> m_abstract_rules; // hold all of the rules
// rule applies to mate too
bool m_applies_to_mate;
// how many reads pass this MiniRule
size_t m_count;
};
/** A full set of rules across any number of regions
*
* Stores the entire set of ReadFilter, each defined on a unique interval.
* A single ReadFilterCollection object is sufficient to store any combination of rules,
* and is the highest in the rule hierarchy. (ReadFilterCollection stores ReadFilter
* stores AbstractRules stores FlagRule/Ranges).
*/
class ReadFilterCollection {
public:
/** Construct an empty ReadFilterCollection
* that will pass all reads.
*/
ReadFilterCollection() : m_count(0), m_count_seen(0) {}
/** Create a new filter collection directly from a JSON
* @param script A JSON file or directly as JSON formatted string
* @param h BamHeader to convert chr sequence to id
* @exception invalid_argument if cannot parse JSON
*/
ReadFilterCollection(const std::string& script, const SeqLib::BamHeader& h);
/** Add a new rule to the collection.
* If a read passes this rule, it will be included,
* even if it fails the other filters. Or, if this filter
* has the excluder tag, then if a read passes this filter
* then it will be excluded, regardless of the other filters
*/
void AddReadFilter(const ReadFilter& rf);
/** Provide a global rule set (applies to each filter)
* @param rule A filter specified in JSON format
*/
void addGlobalRule(const std::string& rule);
/** Query a read to see if it passes any one of the
* filters contained in this collection */
bool isValid(const BamRecord &r);
/** Print some basic information about this object */
friend std::ostream& operator<<(std::ostream& out, const ReadFilterCollection &mr);
/** Return a GenomicRegionCollection of all
* of the regions specified by the filters.
* @note This returns the raw regions. It may be useful
* to run mergeOverlappingIntervals on the output to see
* the minimal covered regions.
*/
GRC getAllRegions() const;
/** Return the number of filters in this collection */
size_t size() const { return m_regions.size(); }
/** Return the total number of rules in this collection.
* Filters are composed of collections of rules, and this
* returns the total number of rules (e.g. MAPQ > 30) across
* all of the filters
*/
size_t numRules() const {
size_t num = 0;
for (std::vector<ReadFilter>::const_iterator it = m_regions.begin(); it != m_regions.end(); ++it)
num += it->size();
return num;
}
/** Check that the filter collection includes something.
* If not, then give it a universal includer
*/
void CheckHasIncluder();
// Return the a tab-delimited tally of which filters were satisfied.
// Includes the header:
// total_seen_count total_passed_count region region_passed_count rule rule_passed_count
//
//std::string EmitCounts() const;
private:
// the global rule that all other rules are inherited from
AbstractRule rule_all;
size_t m_count; // passed
size_t m_count_seen; // tested
// store all of the individual filters
std::vector<ReadFilter> m_regions;
bool ParseFilterObject(const std::string& filterName, const Json::Value& filterObject);
};
}
}
#endif
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