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import unittest
import pyslow5 as slow5
import time
import numpy as np
"""
Run from root dir of repo after making pyslow5
python3 -m unittest -v python/test.py
"""
#globals
debug = 0 #TODO: make this an argument with -v
class TestBase(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example.slow5','r', DEBUG=debug)
self.read = self.s5.get_read("r1")
def tearDown(self):
self.s5.close()
def test_class_methods(self):
result = type(self.s5)
self.assertEqual(str(result), "<class 'pyslow5.Open'>")
def test_read_type(self):
self.assertEqual(str(type(self.read)), "<class 'dict'>")
def test_read_id(self):
self.assertEqual(self.read['read_id'], "r1")
def test_read_group(self):
self.assertEqual(self.read['read_group'], 0)
def test_digitisation(self):
self.assertEqual(self.read['digitisation'], 8192.0)
def test_offset(self):
self.assertEqual(self.read['offset'], 23.0)
def test_range(self):
self.assertEqual(self.read['range'], 1467.61)
def test_sampling_rate(self):
self.assertEqual(self.read['sampling_rate'], 4000.0)
def test_len_raw_signal(self):
self.assertEqual(self.read['len_raw_signal'], 59676)
def test_pylen_signal(self):
self.assertEqual(len(self.read['signal']), 59676)
def test_signal(self):
self.assertEqual(sum(self.read['signal'][:10]), sum([1039,588,588,593,586,574,570,585,588,586]))
class TestRandomAccess(unittest.TestCase):
"""
Get data for ANOTHER ONE individual read, random access, check memory
"""
def setUp(self):
self.s5 = slow5.Open('examples/example.slow5','r', DEBUG=debug)
self.read = self.s5.get_read("r1")
self.read = self.s5.get_read("r4", pA=True)
def tearDown(self):
self.s5.close()
def test_read_id(self):
self.assertEqual(self.read['read_id'], "r4")
def test_read_group(self):
self.assertEqual(self.read['read_group'], 0)
def test_digitisation(self):
self.assertEqual(self.read['digitisation'], 8192.0)
def test_offset(self):
self.assertEqual(self.read['offset'], 23.0)
def test_range(self):
self.assertEqual(self.read['range'], 1467.61)
def test_sampling_rate(self):
self.assertEqual(self.read['sampling_rate'], 4000.0)
def test_len_raw_signal(self):
self.assertEqual(self.read['len_raw_signal'], 59670)
def test_pylen_signal(self):
self.assertEqual(len(self.read['signal']), 59670)
def test_signal(self):
self.assertEqual(int(sum([round(i, 2) for i in self.read['signal'][:10]])), int(sum([190.26, 108.92, 109.46, 109.1, 107.67, 108.39, 108.75, 109.1, 111.07, 108.39])))
class TestAUX(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
self.read = self.s5.get_read("r1", aux=["read_number", "start_mux", "noExistTest"])
def tearDown(self):
self.s5.close()
def test_read_id(self):
self.assertEqual(self.read['read_id'], "r1")
def test_read_number(self):
self.assertEqual(self.read['read_number'], 2287)
def test_start_mux(self):
self.assertEqual(self.read['start_mux'], 2)
def test_nonExistant_aux(self):
self.assertIs(self.read['noExistTest'], None)
class TestSequentialRead(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
self.reads = self.s5.seq_reads()
def tearDown(self):
self.s5.close()
def test_seq_reads(self):
results = ['r0', 'r1', 'r2', 'r3', 'r4', 'r5', '0a238451-b9ed-446d-a152-badd074006c4', '0d624d4b-671f-40b8-9798-84f2ccc4d7fc']
for i, read in enumerate(self.reads):
with self.subTest(i=i, read=read['read_id']):
self.assertEqual(read['read_id'], results[i])
class TestYieldRead(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example.slow5','r', DEBUG=debug)
def tearDown(self):
self.s5.close()
def test_base_reads(self):
read_list = ["r1", "r3", "r5", "r2", "r1"]
results = ["r1", "r3", "r5", "r2", "r1"]
selected_reads = self.s5.get_read_list(read_list)
for i, read in enumerate(selected_reads):
if read is None:
sate = None
else:
state = read['read_id']
with self.subTest(i=i, read=state):
if read is None:
self.assertEqual(read, results[i])
else:
self.assertEqual(read['read_id'], results[i])
def test_reads_with_no_exist(self):
read_list = ["r1", "r3", "null_read", "r5", "r2", "r1"]
results = ["r1", "r3", None, "r5", "r2", "r1"]
selected_reads = self.s5.get_read_list(read_list)
for i, read in enumerate(selected_reads):
if read is None:
sate = None
else:
state = read['read_id']
with self.subTest(i=i, read=state):
if read is None:
self.assertEqual(read, results[i])
else:
self.assertEqual(read['read_id'], results[i])
class TestHeaders(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example.slow5','r', DEBUG=debug)
def tearDown(self):
self.s5.close()
def test_get_header_names(self):
results = ['asic_id', 'asic_id_eeprom', 'asic_temp', 'auto_update', 'auto_update_source',
'bream_core_version', 'bream_is_standard', 'bream_map_version', 'bream_ont_version',
'bream_prod_version', 'bream_rnd_version', 'device_id', 'exp_script_name', 'exp_script_purpose',
'exp_start_time', 'experiment_kit', 'experiment_type', 'file_version', 'filename', 'flow_cell_id',
'heatsink_temp', 'hostname', 'installation_type', 'local_firmware_file', 'operating_system',
'protocol_run_id', 'protocols_version', 'run_id', 'sample_frequency', 'usb_config',
'user_filename_input', 'version']
names = self.s5.get_header_names()
self.assertEqual(names, results)
def test_get_all_headers(self):
results = ['asic_id', 'asic_id_eeprom', 'asic_temp', 'auto_update', 'auto_update_source',
'bream_core_version', 'bream_is_standard', 'bream_map_version', 'bream_ont_version',
'bream_prod_version', 'bream_rnd_version', 'device_id', 'exp_script_name', 'exp_script_purpose',
'exp_start_time', 'experiment_kit', 'experiment_type', 'file_version', 'filename', 'flow_cell_id',
'heatsink_temp', 'hostname', 'installation_type', 'local_firmware_file', 'operating_system',
'protocol_run_id', 'protocols_version', 'run_id', 'sample_frequency', 'usb_config',
'user_filename_input', 'version']
headers = self.s5.get_all_headers()
self.assertEqual(list(headers.keys()), results)
def test_get_all_headers(self):
attr = "flow_cell_id"
result = ""
val = self.s5.get_header_value(attr)
self.assertEqual(val, result)
def test_get_all_headers(self):
attr = "exp_start_time"
result = ""
val = self.s5.get_header_value(attr)
self.assertEqual(val, result)
def test_get_all_headers(self):
attr = "heatsink_temp"
result = ""
val = self.s5.get_header_value(attr)
self.assertEqual(val, result)
def test_get_all_headers(self):
results = ["3574887596", "0", "29.2145729", "1", "https://mirror.oxfordnanoportal.com/software/MinKNOW/", "1.1.20.1",
"1", "1.1.20.1", "1.1.20.1", "1.1.20.1", "0.1.1", "MN16450", "python/recipes/nc/NC_48Hr_Sequencing_Run_FLO-MIN106_SQK-LSK108.py",
"sequencing_run", "1479433093", "genomic_dna", "customer_qc", "1", "deamernanopore_20161117_fnfab43577_mn16450_sequencing_run_ma_821_r9_4_na12878_11_17_16_88738",
"FAB43577", "33.9921875", "DEAMERNANOPORE", "map", "0", "Windows 6.2", "a4429838-103c-497f-a824-7dffa72cfd81",
"1.1.20", "d6e473a6d513ec6bfc150c60fd4556d72f0e6d18", "4000", "1.0.11_ONT#MinION_fpga_1.0.1#ctrl#Auto",
"ma_821_r9.4_na12878_11_17_16", "1.1.20"]
names = self.s5.get_header_names()
for i, attr in enumerate(names):
with self.subTest(i=i, attr=attr):
val = self.s5.get_header_value(attr)
self.assertEqual(val, results[i])
class TestAuxAll(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
def tearDown(self):
self.s5.close()
def test_get_aux_names(self):
result = ['channel_number', 'median_before', 'read_number', 'start_mux', 'start_time']
aux_names = self.s5.get_aux_names()
self.assertEqual(aux_names, result)
def test_get_aux_types(self):
result = [22, 9, 2, 4, 7]
aux_types = self.s5.get_aux_types()
aux_names = self.s5.get_aux_names()
self.assertEqual(aux_types, result)
def test_get_read_all_aux(self):
results = ['391', 260.557264, 2287, 2, 36886851]
read = self.s5.get_read("0d624d4b-671f-40b8-9798-84f2ccc4d7fc", aux="all")
aux_names = self.s5.get_aux_names()
for i, name in enumerate(aux_names):
with self.subTest(i=i, attr=name):
self.assertEqual(read[name], results[i])
def test_seq_reads_pA_aux_all(self):
results = [['r0', 1106.3899999999999, 78470500],
['r1', 1585.4299999999998, 36886851],
['r2', 1106.3899999999999, 78470500],
['r3', 1585.4299999999998, 36886851],
['r4', 1106.3899999999999, 78470500],
['r5', 1585.4299999999998, 36886851],
['0a238451-b9ed-446d-a152-badd074006c4', 1106.3899999999999, 78470500],
['0d624d4b-671f-40b8-9798-84f2ccc4d7fc', 1585.4299999999998, 36886851]]
reads = self.s5.seq_reads(pA=True, aux='all')
for i, read in enumerate(reads):
with self.subTest(i=i, attr=read['read_id']):
self.assertEqual(read['read_id'], results[i][0])
self.assertEqual(int(sum([round(i, 2) for i in read['signal'][:10]])), int(results[i][1]))
self.assertEqual(read['start_time'] ,results[i][2])
class TestWrite(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
self.F = slow5.Open('examples/example_write.slow5','w', DEBUG=debug)
def tearDown(self):
self.s5.close()
self.F.close()
def test_get_empty_header(self):
results = {"asic_id": None,
"asic_id_eeprom": None,
"asic_temp": None,
"asic_version": None,
"auto_update": None,
"auto_update_source": None,
"barcoding_enabled": None,
"bream_is_standard": None,
"configuration_version": None,
"device_id": None,
"device_type": None,
"distribution_status": None,
"distribution_version": None,
"exp_script_name": None,
"exp_script_purpose": None,
"exp_start_time": None,
"experiment_duration_set": None,
"flow_cell_id": None,
"flow_cell_product_code": None,
"guppy_version": None,
"heatsink_temp": None,
"hostname": None,
"installation_type": None,
"local_basecalling": None,
"operating_system": None,
"package": None,
"protocol_group_id": None,
"protocol_run_id": None,
"protocol_start_time": None,
"protocols_version": None,
"run_id": None,
"sample_frequency": None,
"sample_id": None,
"sequencing_kit": None,
"usb_config": None,
"version": None,
"hublett_board_id": None,
"satellite_firmware_version": None}
header = self.F.get_empty_header()
for i, head in enumerate(header):
with self.subTest(i=i, attr=head):
self.assertEqual(header[head], results[head])
def test_write_header(self):
# This doesn't actually write the header, but adds it to object
# Header is actually written when writing first record
results = 0
header = self.F.get_empty_header()
counter = 0
for i in header:
header[i] = "test_{}".format(counter)
counter += 1
ret = self.F.write_header(header)
self.assertEqual(ret, results)
def test_get_empty_record(self):
results = {"read_id": None,
"read_group": 0,
"digitisation": None,
"offset": None,
"range": None,
"sampling_rate": None,
"len_raw_signal": None,
"signal": None}
record = self.F.get_empty_record()
for i, rec in enumerate(record):
with self.subTest(i=i, attr=rec):
self.assertEqual(record[rec], results[rec])
def test_write_records(self):
results = [0]*8
ret_list = []
header = self.F.get_empty_header()
counter = 0
for i in header:
header[i] = "test_{}".format(counter)
counter += 1
ret = self.F.write_header(header)
reads = self.s5.seq_reads()
for read in reads:
record = self.F.get_empty_record()
for i in read:
if i in record:
record[i] = read[i]
ret = self.F.write_record(record)
ret_list.append(ret)
self.assertEqual(ret_list, results)
class testMultiThreading(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
def tearDown(self):
self.s5.close()
def test_seq_reads_multi(self):
results = [['r0', 1106.3899999999999, 78470500],
['r1', 1585.4299999999998, 36886851],
['r2', 1106.3899999999999, 78470500],
['r3', 1585.4299999999998, 36886851],
['r4', 1106.3899999999999, 78470500],
['r5', 1585.4299999999998, 36886851],
['0a238451-b9ed-446d-a152-badd074006c4', 1106.3899999999999, 78470500],
['0d624d4b-671f-40b8-9798-84f2ccc4d7fc', 1585.4299999999998, 36886851]]
reads = self.s5.seq_reads_multi(threads=2, batchsize=3, pA=True, aux='all')
for i, read in enumerate(reads):
with self.subTest(i=i, attr=read['read_id']):
print(read['read_id'])
self.assertEqual(read['read_id'], results[i][0])
self.assertEqual(int(sum([round(i, 2) for i in read['signal'][:10]])), int(results[i][1]))
self.assertEqual(read['start_time'] ,results[i][2])
class testMultiThreadingRandom(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
def tearDown(self):
self.s5.close()
def test_get_reads_multi(self):
results = [['r0', 1106.3899999999999, 78470500],
['r1', 1585.4299999999998, 36886851],
['r2', 1106.3899999999999, 78470500],
['r3', 1585.4299999999998, 36886851],
['r4', 1106.3899999999999, 78470500],
['r5', 1585.4299999999998, 36886851],
['0a238451-b9ed-446d-a152-badd074006c4', 1106.3899999999999, 78470500],
['0d624d4b-671f-40b8-9798-84f2ccc4d7fc', 1585.4299999999998, 36886851]]
read_list = [i[0] for i in results]
reads = self.s5.get_read_list_multi(read_list, threads=2, batchsize=3, pA=True, aux='all')
for i, read in enumerate(reads):
with self.subTest(i=i, attr=read['read_id']):
print(read['read_id'])
self.assertEqual(read['read_id'], results[i][0])
self.assertEqual(int(sum([round(i, 2) for i in read['signal'][:10]])), int(results[i][1]))
self.assertEqual(read['start_time'] ,results[i][2])
class TestWriteData(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
self.F = slow5.Open('examples/example_write.slow5','r', DEBUG=debug)
def tearDown(self):
self.s5.close()
self.F.close()
def test_write_matches_read(self):
reads = self.s5.seq_reads()
for read in reads:
wread = self.F.get_read(read["read_id"])
for i, rec in enumerate(wread):
# TODO: remove this when read_groups are added
with self.subTest(i=i, attr=rec):
if rec == "read_group":
continue
if rec == "signal":
self.assertEqual(int(sum([round(i, 2) for i in wread[rec][:10]])), int(sum([round(i, 2) for i in read[rec][:10]])))
else:
self.assertEqual(wread[rec], read[rec])
class TestWriteAux(unittest.TestCase):
def setUp(self):
self.s5 = slow5.Open('examples/example2.slow5','r', DEBUG=debug)
self.F = slow5.Open('examples/example_write_aux.slow5','w', DEBUG=debug)
def tearDown(self):
self.s5.close()
self.F.close()
def test_get_empty_aux(self):
results = {"channel_number": None,
"median_before": None,
"read_number": None,
"start_mux": None,
"start_time": None,
"end_reason": None}
_, aux = self.F.get_empty_record(aux=True)
for i, rec in enumerate(aux):
with self.subTest(i=i, attr=rec):
self.assertEqual(aux[rec], results[rec])
def test_write_records_aux(self):
results = [0]*8
ret_list = []
header = self.F.get_empty_header()
counter = 0
for i in header:
header[i] = "test_{}".format(counter)
counter += 1
ret = self.F.write_header(header)
reads = self.s5.seq_reads(aux='all')
for read in reads:
record, aux = self.F.get_empty_record(aux=True)
for i in read:
if i in record:
record[i] = read[i]
if i in aux:
aux[i] = read[i]
ret = self.F.write_record(record, aux)
ret_list.append(ret)
self.assertEqual(ret_list, results)
# def test_bad_type(self):
# data = "banana"
# with self.assertRaises(TypeError):
# result = sum(data)
if __name__ == '__main__':
unittest.main()
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