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// an example programme is for a lazy programmer. see mt.c instead.
// uses the optional multi-threaded API under the lazy mode to fetch batches of records in parallel
#include <assert.h>
#include <math.h>
#include <pthread.h>
#include <stdio.h>
#include <stdlib.h>
#include <string.h>
#include <slow5/slow5.h>
#include <slow5/slow5_mt.h>
#define FILE_PATH "test.blow5" //for writing and reading
void sequential_read_func(); //function to read records sequentially
void random_read_func(); //function to read records randomly (lot of read IDs)
void write_func(); //function to write records
int main(){
write_func();
sequential_read_func();
random_read_func();
return 0;
}
void sequential_read_func(){
slow5_file_t *sp = slow5_open(FILE_PATH,"r");
if(sp==NULL){
fprintf(stderr,"Error in opening file\n");
exit(EXIT_FAILURE);
}
slow5_rec_t **rec = NULL;
int ret=0;
int batch_size = 2048;
int num_thread = 8;
while(1){
ret = slow5_get_next_batch_lazy(&rec,sp,batch_size,num_thread);
for(int i=0;i<ret;i++){
uint64_t len_raw_signal = rec[i]->len_raw_signal;
printf("%s\t%ld\n",rec[i]->read_id,len_raw_signal);
}
slow5_free_batch_lazy(&rec,ret);
if(ret<batch_size){ //this indicates nothing left to read //need to handle errors
break;
}
}
slow5_close(sp);
return;
}
void random_read_func(){
slow5_file_t *sp = slow5_open(FILE_PATH,"r");
if(sp==NULL){
fprintf(stderr,"Error in opening file\n");
exit(EXIT_FAILURE);
}
slow5_rec_t **rec = NULL;
int ret = slow5_idx_load(sp);
if(ret<0){
fprintf(stderr,"Error in loading index\n");
exit(EXIT_FAILURE);
}
int num_rid = 4;
int num_thread = 2;
char *rid[num_rid];
rid[0]="read_id_50";
rid[1]="read_id_3999",
rid[2]="read_id_0";
rid[3]="read_id_4";
ret = slow5_get_batch_lazy(&rec, sp, rid, num_rid, num_thread);
assert(ret==num_rid);
for(int i=0;i<ret;i++){
uint64_t len_raw_signal = rec[i]->len_raw_signal;
printf("%s\t%ld\n",rec[i]->read_id,len_raw_signal);
}
slow5_free_batch_lazy(&rec,ret);
slow5_idx_unload(sp);
slow5_close(sp);
return;
}
void write_func(){
slow5_file_t *sf = slow5_open(FILE_PATH,"w");
if(sf==NULL){
fprintf(stderr,"Error in opening file\n");
exit(EXIT_FAILURE);
}
//set zstd record compression, svb-zd signal compression
// if(slow5_set_press(sf, SLOW5_COMPRESS_ZSTD, SLOW5_COMPRESS_SVB_ZD) < 0){ //
// fprintf(stderr,"Error setting compression method!\n");
// exit(EXIT_FAILURE);
// }
/*********************** Header ******************/
slow5_hdr_t *header=sf->header;
//add a header group attribute called run_id
if (slow5_hdr_add_attr("run_id", header) != 0){
fprintf(stderr,"Error adding run_id attribute\n");
exit(EXIT_FAILURE);
}
//add another header group attribute called asic_id
if (slow5_hdr_add_attr("asic_id", header) != 0){
fprintf(stderr,"Error adding asic_id attribute\n");
exit(EXIT_FAILURE);
}
//set the run_id attribute to "run_0" for read group 0
if (slow5_hdr_set("run_id", "run_0", 0, header) != 0){
fprintf(stderr,"Error setting run_id attribute in read group 0\n");
exit(EXIT_FAILURE);
}
//set the asic_id attribute to "asic_0" for read group 1
if (slow5_hdr_set("asic_id", "asic_id_0", 0, header) != 0){
fprintf(stderr,"Error setting asic_id attribute in read group 0\n");
exit(EXIT_FAILURE);
}
//add auxilliary field: channel number
if (slow5_aux_add("channel_number", SLOW5_STRING, sf->header)!=0){
fprintf(stderr,"Error adding channel_number auxilliary field\n");
exit(EXIT_FAILURE);
}
//add axuilliary field: median_before
if (slow5_aux_add("median_before", SLOW5_DOUBLE, sf->header)!=0){
fprintf(stderr,"Error adding median_before auxilliary field\n");
exit(EXIT_FAILURE);
}
//add axuilliary field: read_number
if(slow5_aux_add("read_number", SLOW5_INT32_T, sf->header)!=0){
fprintf(stderr,"Error adding read_number auxilliary field\n");
exit(EXIT_FAILURE);
}
//add axuilliary field: start_mux
if(slow5_aux_add("start_mux", SLOW5_UINT8_T, sf->header)!=0){
fprintf(stderr,"Error adding start_mux auxilliary field\n");
exit(EXIT_FAILURE);
}
//add auxilliary field: start_time
if(slow5_aux_add("start_time", SLOW5_UINT64_T, sf->header)!=0){
fprintf(stderr,"Error adding start_time auxilliary field\n");
exit(EXIT_FAILURE);
}
if(slow5_hdr_write(sf) < 0){
fprintf(stderr,"Error writing header!\n");
exit(EXIT_FAILURE);
}
slow5_rec_t *rec[4000];
int ret=0;
int batch_size = 4000;
int num_thread = 8;
/******************* SLOW5 records ************************/
for(int i=0;i<batch_size;i++){
slow5_rec_t *slow5_record = rec[i] = slow5_rec_init();
if(slow5_record == NULL){
fprintf(stderr,"Could not allocate space for a slow5 record.");
exit(EXIT_FAILURE);
}
//primary fields
char tmp_read_id[100];
sprintf(tmp_read_id,"read_id_%d",i);
slow5_record -> read_id = strdup(tmp_read_id);
if(slow5_record->read_id == NULL){
fprintf(stderr,"Could not allocate space for strdup.");
exit(EXIT_FAILURE);
}
slow5_record -> read_id_len = strlen(slow5_record -> read_id);
slow5_record -> read_group = 0;
slow5_record -> digitisation = 4096.0;
slow5_record -> offset = 3.0+i;
slow5_record -> range = 10.0+i;
slow5_record -> sampling_rate = 4000.0;
slow5_record -> len_raw_signal = 10+i;
slow5_record -> raw_signal = malloc(sizeof(int16_t)*(10+i));
if(slow5_record->raw_signal == NULL){
fprintf(stderr,"Could not allocate space for raw signal.");
exit(EXIT_FAILURE);
}
for(int j=0;j<10+i;j++){
slow5_record->raw_signal[j] = j+i;
}
//auxiliary fileds
char *channel_number = "channel_number";
double median_before = 0.1+i;
int32_t read_number = 10+i;
uint8_t start_mux = (1+i)%4;
uint64_t start_time = 100+i;
if(slow5_aux_set_string(slow5_record, "channel_number", channel_number, sf->header)!=0){
fprintf(stderr,"Error setting channel_number auxilliary field\n");
exit(EXIT_FAILURE);
}
if(slow5_aux_set(slow5_record, "median_before", &median_before, sf->header)!=0){
fprintf(stderr,"Error setting median_before auxilliary field\n");
exit(EXIT_FAILURE);
}
if(slow5_aux_set(slow5_record, "read_number", &read_number, sf->header)!=0){
fprintf(stderr,"Error setting read_number auxilliary field\n");
exit(EXIT_FAILURE);
}
if(slow5_aux_set(slow5_record, "start_mux", &start_mux, sf->header)!=0){
fprintf(stderr,"Error setting start_mux auxilliary field\n");
exit(EXIT_FAILURE);
}
if(slow5_aux_set(slow5_record, "start_time", &start_time, sf->header)!=0){
fprintf(stderr,"Error setting start_time auxilliary field\n");
exit(EXIT_FAILURE);
}
}
//end of record setup
ret = slow5_write_batch_lazy(rec,sf,batch_size,num_thread);
if(ret<batch_size){
fprintf(stderr,"Writing failed\n");
exit(EXIT_FAILURE);
}
slow5_close(sf);
for(int i=0;i<batch_size;i++){
slow5_rec_free(rec[i]);
}
return;
}
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