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/*
* Copyright (C) 2010 Regents of the University of Michigan
*
* This program is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
#ifndef __SAM_FILE_HEADER_H__
#define __SAM_FILE_HEADER_H__
#include <map>
#include <stdint.h>
#include "SamReferenceInfo.h"
#include "SamHeaderHD.h"
#include "SamHeaderSQ.h"
#include "SamHeaderRG.h"
#include "SamHeaderPG.h"
/// This class allows a user to get/set the fields in a SAM/BAM Header.
/// Sam/Bam headers contain comments and multiple SamHeaderRecords
/// (HD, SQs, RGs, PGs) comprised of tag/value pairs with each tag only
/// appearing once within a specific record.
class SamFileHeader
{
public:
SamFileHeader();
~SamFileHeader();
/////////////////////////////
/// @name Copying a Header
/// These methods are ways of copying the contents of one header into
/// another one.
//@{
/// Copy Constructor copies the specified header into this one.
SamFileHeader(const SamFileHeader& header);
/// Overload operator = to copy the passed in header into this header.
SamFileHeader & operator = (const SamFileHeader& header);
/// Copy method copies the passed in header into this header.
/// Returns true if at least one header line was successfully copied.
bool copy(const SamFileHeader& header);
//@}
/// Initialize the header.
void resetHeader();
/////////////////////////////
/// @name Get the Entire Header
/// Get the entire header as a single string.
//@{
/// Set the passed in string to the entire header string, clearing its
/// current contents.
/// \return true if successfully set (even if set to "")
bool getHeaderString(std::string& header) const;
//@}
/// Get the reference ID for the specified reference name (chromosome).
/// If addID is set to true, a reference id will be created for the
/// referenceName if one does not already exist. If addID is set to
/// false (default), it will return SamReferenceInfo::NO_REF_ID.
int getReferenceID(const String & referenceName, bool addID = false);
/// Get the reference ID for the specified reference name (chromosome).
/// If addID is set to true, a reference id will be created for the
/// referenceName if one does not already exist. If addID is set to
/// false (default), it will return SamReferenceInfo::NO_REF_ID.
int getReferenceID(const char* referenceName, bool addID = false);
/// Return the reference name (chromosome) for the specified reference id.
const String & getReferenceLabel(int id) const;
/// Get the Reference Information
const SamReferenceInfo& getReferenceInfo() const;
// Get the Reference Information for updating separately when reading
// BAMs...should only be called by BamInterface.
SamReferenceInfo& getReferenceInfoForBamInterface();
////////////////////////////////////////////////////////////////////////
// Set Values in the header
////////////////////////////////////////////////////////////////////////
/////////////////////////////////////////
/// @name Adding an entire header/comment line.
/// These methods are ways of adding an entire header line at once.
//@{
/// Add a header line that is just one tag with a const char* value.
/// Note: This method will only do one tag per type on a line, so if a
/// type has multiple tags, the whole line needs to be added at once,
/// and a different method should be used.
bool addHeaderLine(const char* type, const char* tag, const char* value);
/// Add a header line that is already preformatted in a const char*.
/// Returns true if at least one header line was successfully added.
bool addHeaderLine(const char* headerLine);
/// Add a header that is already preformatted in a const char*.
/// Returns true if at least one header line was successfully added.
bool addHeader(const char* header);
/// Add the specified comment to the header (do not include "@CO" or "\n").
/// \return true if successfully added, false if not.
bool addComment(const char* comment);
//@}
/////////////////////////////////////////
/// @name Set/Add/Remove a Single Tag
/// The passed in tag should be the two character SAM tag as defined
/// in the SAM spec. A tag is removed from the header record by setting
/// it to "". For the SQ and RG header types, the key tags (SN for SQ
/// and ID for RG) may not be modified or removed once set. This is
/// because these values are used as a lookup key for the header record,
/// so the entire record must be removed.
//@{
// // Set the specified header type tag to the specified value in the
// // header with the specified keyID. keyID must be specified when
// // type = SQ, RG, or PG.
// bool setTag(SamHeaderRecord::SamHeaderRecordType type, const char* tag,
// const char* value, const char* keyID = NULL);
/// Set the specified tag to the specified value in the HD header, remove
/// the tag by specifying value="".
/// \return true if the tag was successfully set, false if not.
bool setHDTag(const char* tag, const char* value);
/// Set the specified tag to the specified value in the SQ header with
/// the specified name, remove the tag by specifying value="". If the
/// header does not yet exist, the tag must be "LN" and the header is added
/// with the specified LN value and the SN value passed in name.
/// The SN & LN tags may not be modified or removed after they are
/// set unless the entire record is deleted.
/// \return true if the tag was successfully set, false if not.
bool setSQTag(const char* tag, const char* value, const char* name);
/// Set the specified tag to the specified value in the RG header with
/// the specified id, remove the tag by specifying value="". If the
/// header does not yet exist, the header is added and so is the ID tag
/// with the value set to the passed in id. The ID tag may not be
/// modified or removed after it is set unless the entire record is deleted.
/// \return true if the tag was successfully set, false if not.
bool setRGTag(const char* tag, const char* value, const char* id);
/// Set the specified tag to the specified value in the PG header with
/// the specified id, remove the tag by specifying value="". If the
/// header does not yet exist, the header is added and so is the ID tag
/// with the value set to the passed in id. The ID tag may not be
/// modified or removed after it is set unless the entire record is deleted.
/// \return true if the tag was successfully set, false if not.
bool setPGTag(const char* tag, const char* value, const char* id);
//@}
/////////////////////////////////////////
/// @name Add an Already Setup SamHeaderRecord
/// NOTE: These methods add a pointer to the passed in record.
/// The header record will be deleted when it's cleaned up from this header.
/// NOTE: Do NOT delete the passed in record, the SamFileHeader class
/// takes care of that itself.
//@{
/// Add the HD record to the header.
/// Note: it adds a pointer to the passed in header record. The header
/// record will be deleted when it is cleaned up from this header.
/// \return true if the record was successfully added, false otherwise.
bool addHD(SamHeaderHD* hd);
/// Add the SQ record to the header.
/// Note: it adds a pointer to the passed in header record. The header
/// record will be deleted when it is cleaned up from this header.
/// \return true if the record was successfully added, false otherwise.
bool addSQ(SamHeaderSQ* sq);
/// Add the RG record to the header.
/// Note: it adds a pointer to the passed in header record. The header
/// record will be deleted when it is cleaned up from this header.
/// \return true if the record was successfully added, false otherwise.
bool addRG(SamHeaderRG* rg);
/// Add the PG record to the header.
/// Note: it adds a pointer to the passed in header record. The header
/// record will be deleted when it is cleaned up from this header.
/// \return true if the record was successfully added, false otherwise.
bool addPG(SamHeaderPG* pg);
/// Add a copy of the specified header record to the header.
/// Note: it creates a new header record that is identical to the specified
/// one and adds it to the header. The passed in pointer will not be
/// deleted due to this.
/// \return true if the record was successfully added, false otherwise.
bool addRecordCopy(const SamHeaderRecord& hdrRec);
//@}
////////////////////////////////////////////////////////////////////////
/// @name Remove an Entire Header Record
//@{
/// Remove the HD record.
/// \return true if successfully removed or did not exist, false if
/// the record still exists.
bool removeHD();
/// Remove SQ record with the specified key.
/// NOTE: Does not remove it from the BAM index.
/// \return true if successfully removed or did not exist, false if
/// the record still exists.
bool removeSQ(const char* name);
/// Remove RG record with the specified key.
/// \return true if successfully removed or did not exist, false if
/// the record still exists.
bool removeRG(const char* id);
/// Remove PG record with the specified key.
/// \return true if successfully removed or did not exist, false if
/// the record still exists.
bool removePG(const char* id);
//@}
////////////////////////////////////////////////////////////////////////
/// @name Get a Specific Tag
/// These methods return the value associated with the specified tag.
/// If the tag does not exist in the record "" is returned.
///
/// For SQ, RG, and PG the value returned is for the tag associated with
/// the specified key (name/id). If a record with that key does not exist
/// or if the tag does not exist for the record with that key, "" is
/// returned.
//@{
/// Returns the value associated with the specified HD tag, returning "" if
/// the tag does not exist in the header.
const char* getHDTagValue(const char* tag);
/// Get the value associated with the specified tag on the SQ line with
/// the specified sequence name, returning "" if the tag or key does
/// not exist.
const char* getSQTagValue(const char* tag, const char* name);
/// Get the value associated with the specified tag on the RG line with
/// the specified read group identifier, returning "" if the tag or key does
/// not exist.
const char* getRGTagValue(const char* tag, const char* id);
/// Get the value associated with the specified tag on the RG line with
/// the specified id, returning "" if the tag or key does
/// not exist.
const char* getPGTagValue(const char* tag, const char* id);
//@}
/// Get the number of SQ objects.
int getNumSQs();
/// Get the number of RG objects.
int getNumRGs();
/// Get the number of PG objects.
int getNumPGs();
////////////////////////////////////////////////////////////////////////
/// @name Get a Specific Header Record
/// These methods return a reference to the specific record that was
/// requested, returning NULL if that record does not exist in the header.
///
/// The returned record can be modified to add/remove some tags.
/// Since a reference is returned, the SamHeaderFile automatically
/// reflects these changes.
//@{
/// Get the HD object, returning NULL if there is no HD record.
SamHeaderHD* getHD();
/// Get the SQ object with the specified sequence name, returning NULL
/// if there is no SQ object with that key.
SamHeaderSQ* getSQ(const char* name);
/// Get the RG object with the specified read group identifier, returning
/// NULL if there is no RG object with that key..
SamHeaderRG* getRG(const char* id);
/// Get the PG object with the specified id, returning NULL
/// if there is no PG object with that key..
SamHeaderPG* getPG(const char* id);
//@}
// //////////////////////////////////
// // Set methods for header fields.
// bool setVersion(const char* version);
// bool setSortOrder(const char* sortOrder);
// bool addSequenceName(const char* sequenceName);
// bool setSequenceLength(const char* keyID, int sequenceLength);
// bool setGenomeAssemblyId(const char* keyID, const char* genomeAssemblyId);
// bool setMD5Checksum(const char* keyID, const char* md5sum);
// bool setURI(const char* keyID, const char* uri);
// bool setSpecies(const char* keyID, const char* species);
// bool addReadGroupID(const char* readGroupID);
// bool setSample(const char* keyID, const char* sample);
// bool setLibrary(const char* keyID, const char* library);
// bool setDescription(const char* keyID, const char* description);
// bool setPlatformUnit(const char* keyID, const char* platform);
// bool setPredictedMedianInsertSize(const char* keyID, const char* isize);
// bool setSequencingCenter(const char* keyID, const char* center);
// bool setRunDate(const char* keyID, const char* runDate);
// bool setTechnology(const char* keyID, const char* technology);
// bool addProgram(const char* programID);
// bool setProgramVersion(const char* keyID, const char* version);
// bool setCommandLine(const char* keyID, const char* commandLine);
// ///////////////////////////////////
// // Get methods for header fields.
// // Returns the number of SQ entries in the header.
// int32_t getSequenceDictionaryCount();
/// Return the Sort Order value that is set in the Header, returning ""
/// if this field does not exist.
const char* getSortOrder();
/// DEPRECATED
const char* getTagSO();
/////////////////////////////
/// @name Get the Header Record/Comment/Line by Record/Comment/Line
/// These methods iterate through the header.
/// NOTE: both getNextHeaderRecord and getNextHeaderLine increment the
/// same iterator. getNextHeaderRecord that takes a header type
/// uses the same iterator as the getNextXXRecord with that type.
/// Otherwise the iterators are independent.
//@{
/// Get the next SQ header record. After all SQ headers have been
/// retrieved, NULL is returned until a reset is called.
/// Independent from getNextHeaderRecord, getNextHeaderLine and the
/// other getNextXXRecord methods and the associated reset methods.
SamHeaderRecord* getNextSQRecord();
/// Get the next RG header record. After all RG headers have been
/// retrieved, NULL is returned until a reset is called.
/// Independent from getNextHeaderRecord, getNextHeaderLine and the
/// other getNextXXRecord methods and the associated reset methods.
SamHeaderRecord* getNextRGRecord();
/// Get the next PG header record. After all PG headers have been
/// retrieved, NULL is returned until a reset is called.
/// Independent from getNextHeaderRecord, getNextHeaderLine and the
/// other getNextXXRecord methods and the associated reset methods.
SamHeaderRecord* getNextPGRecord();
/// Reset to the beginning of the header records so the next call
/// to getNextSQRecord returns the first SQ header record.
void resetSQRecordIter();
/// Reset to the beginning of the header records so the next call
/// to getNextRGRecord returns the first RG header record.
void resetRGRecordIter();
/// Reset to the beginning of the header records so the next call
/// to getNextPGRecord returns the first PG header record.
void resetPGRecordIter();
/// Get the next header record of the specified type starting from the
/// specified index and update the index.
/// After all headers of that type have been retrieved,
/// NULL is returned until a reset is called for that type.
SamHeaderRecord* getNextHeaderRecord(uint32_t& index,
SamHeaderRecord::SamHeaderRecordType headerType);
/// Get the next header record, but not comment line. After all headers
/// have been retrieved, NULL is returned until a reset is called.
/// NOTE: both getNextHeaderRecord and getNextHeaderLine increment the
/// same iterator.
SamHeaderRecord* getNextHeaderRecord();
/// Set the passed in string to the next header line, overwritting
/// the passed in string. If there are no more header lines or there
/// is an error, false is returned and the passed in string is set to ""
/// until a rest is called.
/// NOTE: both getNextHeaderRecord and getNextHeaderLine increment the
/// same iterator.
bool getNextHeaderLine(std::string &headerLine);
/// Reset to the beginning of the header records so the next call
/// to getNextHeaderRecord returns the first header line.
void resetHeaderRecordIter();
/// Append all of the comment lines to the specified string.
void appendCommentLines(std::string &commentLines);
/// Returns the comment on the next comment line. Returns "" if all comment
/// lines have been returned, until resetCommentIter is called.
const char* getNextComment();
/// Resets to the beginning of the comments so getNextComment returns
/// the first comment.
void resetCommentIter();
//@}
/// Get the failure message if a method returned failure.
const char* getErrorMessage() { return(myErrorMessage.c_str()); }
static const std::string EMPTY_RETURN;
private:
// Parse the header string.
bool parseHeader(String& header);
// Parse the specified line of the header.
bool parseHeaderLine(const String& headerLine);
// Set the passed in string to the header line at the specified index.
// It does NOT clear the current contents of header.
bool getHeaderLine(unsigned int index, std::string& header) const;
int16_t makeKey(char ch1, char ch2)
{
return((ch1 << 8) + ch2);
}
// Only one HD type is allowed per file.
SamHeaderHD* myHD;
// There can be multiple SQ Types, indexed by SN.
StringHash mySQs;
// There can be multiple RG Types, indexed by ID.
StringHash myRGs;
// There can be multiple PG types, indexed by ID.
StringHash myPGs;
// Reference Name information
SamReferenceInfo myReferenceInfo;
// Vector of comments
std::vector<std::string> myComments;
std::vector<SamHeaderRecord*> myHeaderRecords;
std::string myErrorMessage;
uint32_t myCurrentSQIndex;
uint32_t myCurrentRGIndex;
uint32_t myCurrentPGIndex;
uint32_t myCurrentHeaderIndex;
uint32_t myCurrentCommentIndex;
};
#endif
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