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/*
* Copyright (C) 2010 Regents of the University of Michigan
*
* This program is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
#include "SamInterface.h"
#include "SamRecordHelper.h"
#include <limits>
#include <stdint.h>
SamInterface::SamInterface()
: myFirstRecord("")
{
}
SamInterface::~SamInterface()
{
}
// Read a SAM file's header.
bool SamInterface::readHeader(IFILE filePtr, SamFileHeader& header,
SamStatus& status)
{
if(filePtr == NULL)
{
// File is not open.
status.setStatus(SamStatus::FAIL_ORDER,
"Cannot read header since the file pointer is null");
return(false);
}
// Clear the passed in header.
header.resetHeader();
int numValid = 0;
int numInvalid = 0;
std::string errorMessages = "";
do {
StringIntHash tags;
StringArray values;
buffer.ReadLine(filePtr);
// Stop reading header lines if at the end of the file or
// if the line is not blank and does not start with an @.
if ( ifeof(filePtr) ||
((buffer.Length() != 0) && (buffer[0] != '@')) )
{
break;
}
// This is a header line, so add it to header.
if(header.addHeaderLine(buffer.c_str()))
{
if(buffer.Length() != 0)
{
++numValid;
}
}
else
{
++numInvalid;
// Failed reading the header.
errorMessages += header.getErrorMessage();
// Skip further processing on this line since it was an error.
continue;
}
} while (1);
// Store the first record since it was read.
myFirstRecord = buffer;
if(numInvalid > 0)
{
if(numValid == 0)
{
std::cerr << "Failed to parse " << numInvalid << " header lines";
std::cerr << ". No valid header lines.\n";
status.setStatus(SamStatus::FAIL_PARSE, errorMessages.c_str());
return(false);
}
}
// Successfully read.
return(true);
}
bool SamInterface::writeHeader(IFILE filePtr, SamFileHeader& header,
SamStatus& status)
{
if((filePtr == NULL) || (filePtr->isOpen() == false))
{
// File is not open, return failure.
status.setStatus(SamStatus::FAIL_ORDER,
"Cannot write header since the file pointer is null");
return(false);
}
////////////////////////////////
// Write the header to the file.
////////////////////////////////
// Construct a string containing the entire header.
std::string headerString = "";
header.getHeaderString(headerString);
int32_t headerLen = headerString.length();
int numWrite = 0;
// Write the header to the file.
numWrite = ifwrite(filePtr, headerString.c_str(), headerLen);
if(numWrite != headerLen)
{
status.setStatus(SamStatus::FAIL_IO,
"Failed to write the SAM header.");
return(false);
}
return(true);
}
void SamInterface::readRecord(IFILE filePtr, SamFileHeader& header,
SamRecord& record,
SamStatus& samStatus)
{
// Initialize the status to success - will be set to false on failure.
samStatus = SamStatus::SUCCESS;
if((filePtr == NULL) || (filePtr->isOpen() == false))
{
// File is not open.
samStatus.addError(SamStatus::FAIL_ORDER,
"filePtr does not point to an open file.");
return;
}
// If the first record has been set, use that and clear it,
// otherwise read the record from the file.
if(myFirstRecord.Length() != 0)
{
buffer = myFirstRecord;
myFirstRecord.Clear();
}
else
{
// Read the next record.
buffer.Clear();
buffer.ReadLine(filePtr);
// If the end of the file and nothing was read, return false.
if ((ifeof(filePtr)) && (buffer.Length() == 0))
{
// end of the file and nothing to process.
samStatus.addError(SamStatus::NO_MORE_RECS,
"No more records in the file.");
return;
}
}
tokens.ReplaceColumns(buffer, '\t');
// Error string for reporting a parsing failure.
String errorString = "";
if (tokens.Length() < 11)
{
errorString = "Too few columns (";
errorString += tokens.Length();
errorString += ") in the Record, expected at least 11.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
return;
}
// Reset the record before setting any fields.
record.resetRecord();
if(!record.setReadName(tokens[0]))
{
samStatus.addError(record.getStatus());
}
long flagInt = 0;
if(!tokens[1].AsInteger(flagInt))
{
errorString = "flag, ";
errorString += tokens[1].c_str();
errorString += ", is not an integer.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if((flagInt < 0) || (flagInt > UINT16_MAX))
{
errorString = "flag, ";
errorString += tokens[1].c_str();
errorString += ", is not between 0 and (2^16)-1 = 65535.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if(!record.setFlag(flagInt))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
if(!record.setReferenceName(header, tokens[2]))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
long posInt = 0;
if(!tokens[3].AsInteger(posInt))
{
errorString = "position, ";
errorString += tokens[3].c_str();
errorString += ", is not an integer.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if((posInt < INT32_MIN) || (posInt > INT32_MAX))
{
// If it is not in this range, it cannot fit into a 32 bit int.
errorString = "position, ";
errorString += tokens[3].c_str();
errorString += ", does not fit in a 32 bit signed int.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if(!record.set1BasedPosition(posInt))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
long mapInt = 0;
if(!tokens[4].AsInteger(mapInt))
{
errorString = "map quality, ";
errorString += tokens[4].c_str();
errorString += ", is not an integer.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if((mapInt < 0) || (mapInt > UINT8_MAX))
{
errorString = "map quality, ";
errorString += tokens[4].c_str();
errorString += ", is not between 0 and (2^8)-1 = 255.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if(!record.setMapQuality(mapInt))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
if(!record.setCigar(tokens[5]))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
if(!record.setMateReferenceName(header, tokens[6]))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
long matePosInt = 0;
if(!tokens[7].AsInteger(matePosInt))
{
errorString = "mate position, ";
errorString += tokens[7].c_str();
errorString += ", is not an integer.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if(!record.set1BasedMatePosition(matePosInt))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
long insertInt = 0;
if(!tokens[8].AsInteger(insertInt))
{
errorString = "insert size, ";
errorString += tokens[8].c_str();
errorString += ", is not an integer.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
}
else if(!record.setInsertSize(insertInt))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
if(!record.setSequence(tokens[9]))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
if(!record.setQuality(tokens[10]))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
// Clear the tag fields.
record.clearTags();
// Add the tags to the record.
for (int i = 11; i < tokens.Length(); i++)
{
String & nugget = tokens[i];
if (nugget.Length() < 6 || nugget[2] != ':' || nugget[4] != ':')
{
// invalid tag format.
errorString = "Invalid Tag Format: ";
errorString += nugget.c_str();
errorString += ", should be cc:c:x*.";
samStatus.addError(SamStatus::FAIL_PARSE,
errorString.c_str());
continue;
}
// Valid tag format.
// Add the tag.
if(!record.addTag((const char *)nugget, nugget[3],
(const char *)nugget + 5))
{
samStatus.addError(record.getStatus().getStatus(),
record.getStatus().getStatusMessage());
}
}
return;
}
SamStatus::Status SamInterface::writeRecord(IFILE filePtr,
SamFileHeader& header,
SamRecord& record,
SamRecord::SequenceTranslation translation)
{
// Store all the fields into a string, then write the string.
String recordString = record.getReadName();
recordString += "\t";
recordString += record.getFlag();
recordString += "\t";
recordString += record.getReferenceName();
recordString += "\t";
recordString += record.get1BasedPosition();
recordString += "\t";
recordString += record.getMapQuality();
recordString += "\t";
recordString += record.getCigar();
recordString += "\t";
recordString += record.getMateReferenceNameOrEqual();
recordString += "\t";
recordString += record.get1BasedMatePosition();
recordString += "\t";
recordString += record.getInsertSize();
recordString += "\t";
recordString += record.getSequence(translation);
recordString += "\t";
recordString += record.getQuality();
// If there are any tags, add a preceding tab.
if(record.getTagLength() != 0)
{
recordString += "\t";
SamRecordHelper::genSamTagsString(record, recordString);
}
recordString += "\n";
// Write the record.
ifwrite(filePtr, recordString.c_str(), recordString.Length());
return(SamStatus::SUCCESS);
}
void SamInterface::ParseHeaderLine(StringIntHash & tags, StringArray & values)
{
tags.Clear();
values.Clear();
tokens.AddColumns(buffer, '\t');
for (int i = 1; i < tokens.Length(); i++)
{
tags.Add(tokens[i].Left(2), i - 1);
values.Push(tokens[i].SubStr(3));
}
}
bool SamInterface::isEOF(IFILE filePtr)
{
if(myFirstRecord.Length() != 0)
{
// First record is set, so return false, not EOF, since we
// know the record still needs to be processed.
return(false);
}
return(GenericSamInterface::isEOF(filePtr));
}
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