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/*
* Copyright (C) 2012-2013 Regents of the University of Michigan
*
* This program is free software: you can redistribute it and/or modify
* it under the terms of the GNU General Public License as published by
* the Free Software Foundation, either version 3 of the License, or
* (at your option) any later version.
*
* This program is distributed in the hope that it will be useful,
* but WITHOUT ANY WARRANTY; without even the implied warranty of
* MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
* GNU General Public License for more details.
*
* You should have received a copy of the GNU General Public License
* along with this program. If not, see <http://www.gnu.org/licenses/>.
*/
#include "Tabix.h"
#include <stdexcept>
#include "StringBasics.h"
Tabix::Tabix()
: IndexBase(),
myChromNamesBuffer(NULL)
{
}
Tabix::~Tabix()
{
if(myChromNamesBuffer != NULL)
{
delete[] myChromNamesBuffer;
myChromNamesBuffer = NULL;
}
}
// Reset the member data for a new index file.
void Tabix::resetIndex()
{
IndexBase::resetIndex();
if(myChromNamesBuffer != NULL)
{
delete[] myChromNamesBuffer;
myChromNamesBuffer = NULL;
}
myChromNamesVector.clear();
}
// Read & parse the specified index file.
StatGenStatus::Status Tabix::readIndex(const char* filename)
{
// Reset the index from anything that may previously be set.
resetIndex();
IFILE indexFile = ifopen(filename, "rb");
// Failed to open the index file.
if(indexFile == NULL)
{
return(StatGenStatus::FAIL_IO);
}
// read the tabix index structure.
// Read the magic string.
char magic[4];
if(ifread(indexFile, magic, 4) != 4)
{
// Failed to read the magic
return(StatGenStatus::FAIL_IO);
}
// If this is not an index file, set num references to 0.
if (magic[0] != 'T' || magic[1] != 'B' || magic[2] != 'I' || magic[3] != 1)
{
// Not a Tabix Index file.
return(StatGenStatus::FAIL_PARSE);
}
// It is a tabix index file.
// Read the number of reference sequences.
if(ifread(indexFile, &n_ref, 4) != 4)
{
// Failed to read.
return(StatGenStatus::FAIL_IO);
}
// Size the references.
myRefs.resize(n_ref);
// Read the Format configuration.
if(ifread(indexFile, &myFormat, sizeof(myFormat)) != sizeof(myFormat))
{
// Failed to read.
return(StatGenStatus::FAIL_IO);
}
// Read the length of the chromosome names.
uint32_t l_nm;
if(ifread(indexFile, &l_nm, sizeof(l_nm)) != sizeof(l_nm))
{
// Failed to read.
return(StatGenStatus::FAIL_IO);
}
// Read the chromosome names.
myChromNamesBuffer = new char[l_nm];
if(ifread(indexFile, myChromNamesBuffer, l_nm) != l_nm)
{
return(StatGenStatus::FAIL_IO);
}
myChromNamesVector.resize(n_ref);
// Parse out the chromosome names.
bool prevNull = true;
int chromIndex = 0;
for(uint32_t i = 0; i < l_nm; i++)
{
if(chromIndex >= n_ref)
{
// already set the pointer for the last chromosome name,
// so stop looping.
break;
}
if(prevNull == true)
{
myChromNamesVector[chromIndex++] = myChromNamesBuffer + i;
prevNull = false;
}
if(myChromNamesBuffer[i] == '\0')
{
prevNull = true;
}
}
for(int refIndex = 0; refIndex < n_ref; refIndex++)
{
// Read each reference.
Reference* ref = &(myRefs[refIndex]);
// Read the number of bins.
if(ifread(indexFile, &(ref->n_bin), 4) != 4)
{
// Failed to read the number of bins.
// Return failure.
return(StatGenStatus::FAIL_PARSE);
}
// Resize the bins.
ref->bins.resize(ref->n_bin + 1);
// Read each bin.
for(int binIndex = 0; binIndex < ref->n_bin; binIndex++)
{
uint32_t binNumber;
// Read in the bin number.
if(ifread(indexFile, &(binNumber), 4) != 4)
{
// Failed to read the bin number.
// Return failure.
return(StatGenStatus::FAIL_IO);
}
// Add the bin to the reference and get the
// pointer back so the values can be set in it.
Bin* binPtr = &(ref->bins[binIndex]);
binPtr->bin = binNumber;
// Read in the number of chunks.
if(ifread(indexFile, &(binPtr->n_chunk), 4) != 4)
{
// Failed to read number of chunks.
// Return failure.
return(StatGenStatus::FAIL_IO);
}
// Read in the chunks.
// Allocate space for the chunks.
uint32_t sizeOfChunkList = binPtr->n_chunk * sizeof(Chunk);
binPtr->chunks = (Chunk*)malloc(sizeOfChunkList);
if(ifread(indexFile, binPtr->chunks, sizeOfChunkList) != sizeOfChunkList)
{
// Failed to read the chunks.
// Return failure.
return(StatGenStatus::FAIL_IO);
}
}
// Read the number of intervals.
if(ifread(indexFile, &(ref->n_intv), 4) != 4)
{
// Failed to read, set to 0.
ref->n_intv = 0;
// Return failure.
return(StatGenStatus::FAIL_IO);
}
// Allocate space for the intervals and read them.
uint32_t linearIndexSize = ref->n_intv * sizeof(uint64_t);
ref->ioffsets = (uint64_t*)malloc(linearIndexSize);
if(ifread(indexFile, ref->ioffsets, linearIndexSize) != linearIndexSize)
{
// Failed to read the linear index.
// Return failure.
return(StatGenStatus::FAIL_IO);
}
}
// Successfully read teh bam index file.
return(StatGenStatus::SUCCESS);
}
bool Tabix::getStartPos(const char* refName, int32_t start,
uint64_t& fileStartPos) const
{
// Look for the reference name in the list.
int refID = 0;
for(refID = 0; refID < n_ref; refID++)
{
if(strcmp(refName, myChromNamesVector[refID]) == 0)
{
// found the reference
break;
}
}
if(refID >= n_ref)
{
// Didn't find the refName, so return false.
return(false);
}
// Look up in the linear index.
if(start < 0)
{
// Negative index, so start at 0.
start = 0;
}
return(getMinOffsetFromLinearIndex(refID, start, fileStartPos));
}
const char* Tabix::getRefName(unsigned int indexNum) const
{
if(indexNum >= myChromNamesVector.size())
{
String message = "ERROR: Out of range on Tabix::getRefName(";
message += indexNum;
message += ")";
throw(std::runtime_error(message.c_str()));
return(NULL);
}
return(myChromNamesVector[indexNum]);
}
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