File: VcfRecord.cpp

package info (click to toggle)
libstatgen 1.0.15-8
  • links: PTS, VCS
  • area: main
  • in suites: forky, sid, trixie
  • size: 4,588 kB
  • sloc: cpp: 49,624; ansic: 1,408; makefile: 320; sh: 60
file content (443 lines) | stat: -rw-r--r-- 11,632 bytes parent folder | download | duplicates (2)
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
/*
 *  Copyright (C) 2010-2012  Regents of the University of Michigan,
 *                           Hyun Min Kang, Matthew Flickenger, Matthew Snyder,
 *                           and Goncalo Abecasis
 *
 *   This program is free software: you can redistribute it and/or modify
 *   it under the terms of the GNU General Public License as published by
 *   the Free Software Foundation, either version 3 of the License, or
 *   (at your option) any later version.
 *
 *   This program is distributed in the hope that it will be useful,
 *   but WITHOUT ANY WARRANTY; without even the implied warranty of
 *   MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.  See the
 *   GNU General Public License for more details.
 *
 *   You should have received a copy of the GNU General Public License
 *   along with this program.  If not, see <http://www.gnu.org/licenses/>.
 */

#include "VcfRecord.h"

VcfRecord::VcfRecord()
{
    reset();
}


VcfRecord::~VcfRecord()
{
}


bool VcfRecord::read(IFILE filePtr, bool siteOnly,
                     VcfRecordDiscardRules& discardRules,
                     VcfSubsetSamples* sampleSubset)
{
    // Clear out any previously set values.
    reset();
    
    if(filePtr == NULL)
    {
        myStatus.setStatus(StatGenStatus::FAIL_ORDER,
                           "Error reading VCF record before opening the file.");
        return(false);
    }

    if(ifeof(filePtr))
    {
        // End of file, just return false.
        return(false);
    }
    
    // Read the chromosome.
    if(!readTilTab(filePtr, myChrom))
    {
        if(myChrom.empty())
        {
            // EOF.
            return(false);
        }
        // Not an empty line.
        myStatus.setStatus(StatGenStatus::FAIL_PARSE, 
                           "Error reading VCF Record CHROM.");
        return(false);
    }
    // Read the 1-based Position
    std::string strPos;
    if(!readTilTab(filePtr, strPos))
    {
        myStatus.setStatus(StatGenStatus::FAIL_PARSE, 
                           "Error reading VCF Record POS.");
        return(false);
    }
    else
    {
        // Read the position, so convert to an integer.
        my1BasedPosNum = atoi(strPos.c_str());
    }
    // Read the ID.
    if(!readTilTab(filePtr, myID))
    {
        myStatus.setStatus(StatGenStatus::FAIL_PARSE, 
                           "Error reading VCF Record ID.");
        return(false);
    }

    if(discardRules.discardForID(myID))
    {
        // Do not keep this id, so consume the rest of the record and
        // return the next record.
        filePtr->discardLine();
        return(read(filePtr, siteOnly, discardRules, sampleSubset));
    }

    // Read the Ref.
    if(!readTilTab(filePtr, myRef))
    {
        myStatus.setStatus(StatGenStatus::FAIL_PARSE, 
                           "Error reading VCF Record REF.");
        return(false);
    }
    // Read the Alt.
    myAltArray.clear();
    if(!readTilTab(filePtr, myAlt))
    {
        myStatus.setStatus(StatGenStatus::FAIL_PARSE, 
                           "Error reading VCF Record ALT.");
        return(false);
    }
    // Read the Qual.
    if(!readTilTab(filePtr, myQual))
    {
        myStatus.setStatus(StatGenStatus::FAIL_PARSE, 
                           "Error reading VCF Record QUAL.");
        return(false);
    }
    else
    {
        if(myQual != ".")
        {
            // Read the quality, so convert to an integer.
            myQualNum = atof(myQual.c_str());
        }
        else
        {
            myQualNum = -1;
        }
    }
    // Read the Filter.
    if(!myFilter.read(filePtr))
    {
        myStatus.setStatus(StatGenStatus::FAIL_PARSE, 
                           "Error reading VCF Record FILTER.");
        return(false);
    }
    // Read the Info (could be the last word in the line or file).
    if(!myInfo.read(filePtr))
    {
        // Found the end of the line after the info field, so return true,
        // successfully read the record.
        return(true);
    }

    if(siteOnly)
    {
        // Do not store genotypes, so just consume the rest of the line.
        filePtr->readTilChar("\n");
    }
    else
    {
        // Not yet at the end of the line, so read the genotype fields
        // (format & samples)
        try
        {
            myGenotype.read(filePtr, sampleSubset);
        }
        catch(std::exception& e)
        {
            myDummyString = "Failed parsing the Genotype Fields of " + myChrom + ":" + 
                std::to_string((long long int)my1BasedPosNum) + " (chr:pos) - " + e.what();
            myStatus.setStatus(StatGenStatus::FAIL_PARSE, myDummyString.c_str());
            return(false);
        }
    }
    // Found the end of the line, return true since all required fields
    // were read.
    return(true);
}


bool VcfRecord::write(IFILE filePtr, bool siteOnly)
{
    if(filePtr == NULL)
    {
        myStatus.setStatus(StatGenStatus::FAIL_ORDER,
                           "Error writing VCF record before opening the file.");
        return(false);
    }

    int numWritten = 0;
    int numExpected = 0;
    if(myChrom.length() == 0)
    {
        numWritten += ifprintf(filePtr, ".\t");
        numExpected += 2;
    }
    else
    {
        numWritten += ifprintf(filePtr, "%s\t", myChrom.c_str());
        numExpected += myChrom.length() + 1;
    }
    if(false) //my1BasedPos.length() == 0)
    {
        numWritten += ifprintf(filePtr, ".\t");
        numExpected += 2;
    }
    else
    {
        std::string strPos = std::to_string((long long int)my1BasedPosNum);
        numWritten += ifprintf(filePtr, "%s\t", strPos.c_str());
        numExpected += strPos.length() + 1;
    }
    if(myID.length() == 0)
    {
        numWritten += ifprintf(filePtr, ".\t");
        numExpected += 2;
    }
    else
    {
        numWritten += ifprintf(filePtr, "%s\t", myID.c_str());
        numExpected += myID.length() + 1;
    }
    if(myRef.length() == 0)
    {
        numWritten += ifprintf(filePtr, ".\t");
        numExpected += 2;
    }
    else
    {
        numWritten += ifprintf(filePtr, "%s\t", myRef.c_str());
        numExpected += myRef.length() + 1;
    }
    if(myAlt.length() == 0)
    {
        numWritten += ifprintf(filePtr, ".\t");
        numExpected += 2;
    }
    else
    {
        numWritten += ifprintf(filePtr, "%s\t", myAlt.c_str());
        numExpected += myAlt.length() + 1;
    }
    if(myQual.length() == 0)
    {
        numWritten += ifprintf(filePtr, ".\t");
        numExpected += 2;
    }
    else
    {
        numWritten += ifprintf(filePtr, "%s\t", myQual.c_str());
        numExpected += myQual.length() + 1;
    }
    const std::string& filterString = myFilter.getString();
    if(filterString.length() == 0)
    {
        numWritten += ifprintf(filePtr, ".\t");
        numExpected += 2;
    }
    else
    {
        numWritten += ifprintf(filePtr, "%s\t", filterString.c_str());
        numExpected += filterString.length() + 1;
    }

    // Write the info.
    bool writeSuccess = myInfo.write(filePtr);

    // Only write the format & genotype if we are not just writing siteOnly
    // data and there is at least one sample 
    if((!siteOnly) && (myGenotype.getNumSamples() != 0))
    {
        writeSuccess &= myGenotype.write(filePtr);
    }

    // Write the new line.
    numWritten += ifprintf(filePtr, "\n");
    numExpected += 1;

    return((numWritten == numExpected) && writeSuccess);
}



void VcfRecord::reset()
{
    myChrom.clear();
    my1BasedPosNum = 0;
    //my1BasedPos.clear();
    myID.clear();
    myRef.clear();
    myAlt.clear();
    myAltArray.clear();
    myQualNum = 0;;
    myQual.clear();
    myFilter.clear();
    myInfo.clear();
    myGenotype.clear();
    myAlleleCount.clear();
    myStatus = StatGenStatus::SUCCESS;
}


// Return the error after a failed call.
const StatGenStatus& VcfRecord::getStatus()
{
    return(myStatus);
}


const char* VcfRecord::getAlleles(unsigned int index)
{
    if(index == 0)
    {
        return(myRef.c_str());
    }
    if(index > getNumAlts())
    {
        // Index out of range.
        // Throw an exception.
        throw(std::runtime_error("VcfRecord::getAlleles called with an index that is greater than the number of alternates."));
        return(NULL);
    }
    // Alternate allele, so return the alternate.
    return(myAltArray.get(index-1).c_str());
}


int VcfRecord::getIntAllele(unsigned int index)
{
    const char* alleles = getAlleles(index);
    switch(alleles[0])
    {
        case 'A':
            return(1);
            break;
        case 'C':
            return(2);
            break;
        case 'G':
            return(3);
            break;
        case 'T':
            return(4);
            break;
        default:
            std::cerr << "VcfRecord::getIntAllele, unknown allele, " 
                      << alleles[0] << std::endl;
    }
    return(0);
}


unsigned int VcfRecord::getNumAlts()
{
    int numAlts = myAltArray.size();
    if(numAlts != 0)
    {
        // Already parsed so just return the number of alternates.
        return(numAlts);
    }
    // Check if it is just '.'.
    if((myAlt.length() == 1) && (myAlt == "."))
    {
        // No alternates.
        return(0);
    }

    // Parse the alternates by looping looking for commas.
    std::string* altStr = &(myAltArray.getNextEmpty());
    for(std::string::iterator iter = myAlt.begin(); iter != myAlt.end(); iter++)
    {
        if(*iter == ',')
        {
            altStr = &(myAltArray.getNextEmpty());
        }
        else
        {
            altStr->push_back(*iter);
        }
    }
    return(myAltArray.size());
}


int VcfRecord::getAlleleCount(unsigned int index,
                              VcfSubsetSamples* sampleSubset)
{
    unsigned int numAlts = getNumAlts();
    if(index > numAlts)
    {
        // Index out of range.
        // Throw an exception.
        throw(std::runtime_error("VcfRecord::getAlleles called with an index that is greater than the number of alternates."));
        return(-1);
    }

    if(myAlleleCount.size() == 0)
    {
        unsigned int gt = 0;
        myAlleleCount.resize(numAlts+1, 0);

        // Loop through the samples, counting the number of each allele.
        for(int sampleNum = 0; sampleNum < getNumSamples(); 
            sampleNum++)
        {
            if((sampleSubset != NULL) &&
               !(sampleSubset->keep(sampleNum)))
            {
                // Skip this sample.
                continue;
            }
            for(int gtNum = 0; gtNum < getNumGTs(sampleNum); gtNum++)
            {
                gt = getGT(sampleNum, gtNum);
                if((gt < 0) || (gt > numAlts))
                {
                    // Out of range GT, so continue to the next gt
                    continue;
                }
                // Increment the minor allele count
                ++myAlleleCount[gt];
            }
        }
    }

    // Alternate allele, so return the alternate.
    return(myAlleleCount[index]);
}


bool VcfRecord::readTilTab(IFILE filePtr, std::string& stringRef)
{
    int charRead = 0;
    while(1)
    {
        charRead = ifgetc(filePtr);
        
        if((charRead == '\n') || (charRead == EOF))
        {
            // Didn't find a tab, found a '\n' or eof
            // It still populated the string with values up
            // until the tab.
            return(false);
        }
        if(charRead == '\t')
        {
            // hit the tab character, so exit the loop.
            break;
        }
        stringRef += charRead;
    }
    return(true);
}