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/*
* The MIT License
*
* Wavefront Alignment Algorithms
* Copyright (c) 2017 by Santiago Marco-Sola <santiagomsola@gmail.com>
*
* This file is part of Wavefront Alignment Algorithms.
*
* Permission is hereby granted, free of charge, to any person obtaining a copy
* of this software and associated documentation files (the "Software"), to deal
* in the Software without restriction, including without limitation the rights
* to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
* copies of the Software, and to permit persons to whom the Software is
* furnished to do so, subject to the following conditions:
*
* The above copyright notice and this permission notice shall be included in all
* copies or substantial portions of the Software.
*
* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
* IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
* FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE
* AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
* LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM,
* OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE
* SOFTWARE.
*
* PROJECT: Wavefront Alignment Algorithms
* AUTHOR(S): Santiago Marco-Sola <santiagomsola@gmail.com>
*/
#include "utils/commons.h"
#include "system/mm_allocator.h"
#include "wavefront_unialign.h"
#include "wavefront.h"
#include "wavefront_attributes.h"
#include "wavefront_offset.h"
#include "wavefront_penalties.h"
#include "wavefront_plot.h"
#include "wavefront_slab.h"
#include "wavefront_components.h"
#include "wavefront_compute.h"
#include "wavefront_compute_affine.h"
#include "wavefront_compute_affine2p.h"
#include "wavefront_compute_edit.h"
#include "wavefront_compute_linear.h"
#include "wavefront_extend.h"
#include "wavefront_backtrace.h"
#include "wavefront_backtrace_buffer.h"
/*
* Configuration
*/
#define SEQUENCES_PADDING 10
/*
* Setup
*/
void wavefront_unialign_status_clear(
wavefront_align_status_t* const align_status) {
align_status->status = WF_STATUS_SUCCESSFUL;
align_status->score = 0;
}
void wavefront_unialigner_system_clear(
wavefront_aligner_t* const wf_aligner) {
// Reset effective limits
wf_aligner->system.max_memory_compact = BUFFER_SIZE_256M;
wf_aligner->system.max_memory_resident = BUFFER_SIZE_256M + BUFFER_SIZE_256M;
switch (wf_aligner->memory_mode) {
case wavefront_memory_med:
wf_aligner->system.max_partial_compacts = 4;
break;
case wavefront_memory_low:
wf_aligner->system.max_partial_compacts = 1;
break;
default:
break;
}
// Profile
timer_reset(&wf_aligner->system.timer);
}
/*
* Resize
*/
void wavefront_unialign_resize(
wavefront_aligner_t* const wf_aligner,
const char* const pattern,
const int pattern_length,
const char* const text,
const int text_length,
const bool reverse_sequences) {
// Configure sequences and status
wf_aligner->pattern_length = pattern_length;
wf_aligner->text_length = text_length;
if (wf_aligner->match_funct == NULL) {
if (wf_aligner->sequences != NULL) strings_padded_delete(wf_aligner->sequences);
wf_aligner->sequences = strings_padded_new_rhomb(
pattern,pattern_length,text,text_length,
SEQUENCES_PADDING,reverse_sequences,
wf_aligner->mm_allocator);
wf_aligner->pattern = wf_aligner->sequences->pattern_padded;
wf_aligner->text = wf_aligner->sequences->text_padded;
} else {
wf_aligner->sequences = NULL;
wf_aligner->pattern = NULL;
wf_aligner->text = NULL;
}
wavefront_unialign_status_clear(&wf_aligner->align_status);
// Heuristics clear
wavefront_heuristic_clear(&wf_aligner->heuristic);
// Wavefront components
wavefront_components_resize(&wf_aligner->wf_components,
pattern_length,text_length,&wf_aligner->penalties);
// CIGAR
if (wf_aligner->alignment_scope == compute_alignment) {
cigar_resize(wf_aligner->cigar,2*(pattern_length+text_length));
}
// Slab
wavefront_slab_clear(wf_aligner->wavefront_slab);
// System
wavefront_unialigner_system_clear(wf_aligner);
}
/*
* Initialize alignment
*/
void wavefront_unialign_initialize_wavefront_m(
wavefront_aligner_t* const wf_aligner,
const int pattern_length,
const int text_length) {
// Parameters
wavefront_slab_t* const wavefront_slab = wf_aligner->wavefront_slab;
wavefront_components_t* const wf_components = &wf_aligner->wf_components;
const distance_metric_t distance_metric = wf_aligner->penalties.distance_metric;
wavefront_penalties_t* const penalties = &wf_aligner->penalties;
alignment_form_t* const form = &wf_aligner->alignment_form;
// Consider ends-free
const int hi = (penalties->match==0) ? form->text_begin_free : 0;
const int lo = (penalties->match==0) ? -form->pattern_begin_free : 0;
// Compute dimensions
int effective_lo, effective_hi;
wavefront_compute_limits_output(wf_aligner,lo,hi,&effective_lo,&effective_hi);
// Initialize end2end (wavefront zero)
wf_components->mwavefronts[0] = wavefront_slab_allocate(wavefront_slab,effective_lo,effective_hi);
wf_components->mwavefronts[0]->offsets[0] = 0;
wf_components->mwavefronts[0]->lo = lo;
wf_components->mwavefronts[0]->hi = hi;
// Store initial BT-piggypack element
if (wf_components->bt_piggyback) {
const bt_block_idx_t block_idx = wf_backtrace_buffer_init_block(wf_components->bt_buffer,0,0);
wf_components->mwavefronts[0]->bt_pcigar[0] = 0;
wf_components->mwavefronts[0]->bt_prev[0] = block_idx;
}
// Initialize ends-free
if (form->span == alignment_endsfree && penalties->match == 0) {
// Text begin-free
const int text_begin_free = form->text_begin_free;
int h;
for (h=1;h<=text_begin_free;++h) {
const int k = DPMATRIX_DIAGONAL(h,0);
wf_components->mwavefronts[0]->offsets[k] = DPMATRIX_OFFSET(h,0);
if (wf_components->bt_piggyback) {
const bt_block_idx_t block_idx = wf_backtrace_buffer_init_block(wf_components->bt_buffer,0,h);
wf_components->mwavefronts[0]->bt_pcigar[k] = 0;
wf_components->mwavefronts[0]->bt_prev[k] = block_idx;
}
}
// Pattern begin-free
const int pattern_begin_free = form->pattern_begin_free;
int v;
for (v=1;v<=pattern_begin_free;++v) {
const int k = DPMATRIX_DIAGONAL(0,v);
wf_components->mwavefronts[0]->offsets[k] = DPMATRIX_OFFSET(0,v);
if (wf_components->bt_piggyback) {
const bt_block_idx_t block_idx = wf_backtrace_buffer_init_block(wf_components->bt_buffer,v,0);
wf_components->mwavefronts[0]->bt_pcigar[k] = 0;
wf_components->mwavefronts[0]->bt_prev[k] = block_idx;
}
}
}
// Nullify unused WFs
if (distance_metric <= gap_linear) return;
wf_components->d1wavefronts[0] = NULL;
wf_components->i1wavefronts[0] = NULL;
if (distance_metric==gap_affine) return;
wf_components->d2wavefronts[0] = NULL;
wf_components->i2wavefronts[0] = NULL;
}
void wavefront_unialign_initialize_wavefronts(
wavefront_aligner_t* const wf_aligner,
const int pattern_length,
const int text_length) {
// Parameters
wavefront_slab_t* const wavefront_slab = wf_aligner->wavefront_slab;
wavefront_components_t* const wf_components = &wf_aligner->wf_components;
const distance_metric_t distance_metric = wf_aligner->penalties.distance_metric;
// Init wavefronts
if (wf_aligner->component_begin == affine2p_matrix_M) {
// Initialize
wavefront_unialign_initialize_wavefront_m(wf_aligner,pattern_length,text_length);
// Nullify unused WFs
if (distance_metric <= gap_linear) return;
wf_components->i1wavefronts[0] = NULL;
wf_components->d1wavefronts[0] = NULL;
if (distance_metric==gap_affine) return;
wf_components->i2wavefronts[0] = NULL;
wf_components->d2wavefronts[0] = NULL;
} else {
// Compute dimensions
int effective_lo, effective_hi; // Effective lo/hi
wavefront_compute_limits_output(wf_aligner,0,0,&effective_lo,&effective_hi);
wavefront_t* const wavefront = wavefront_slab_allocate(wavefront_slab,effective_lo,effective_hi);
// Initialize
switch (wf_aligner->component_begin) {
case affine2p_matrix_I1:
wf_components->mwavefronts[0] = NULL;
wf_components->i1wavefronts[0] = wavefront;
wf_components->i1wavefronts[0]->offsets[0] = 0;
wf_components->i1wavefronts[0]->lo = 0;
wf_components->i1wavefronts[0]->hi = 0;
wf_components->d1wavefronts[0] = NULL;
// Nullify unused WFs
if (distance_metric==gap_affine) return;
wf_components->i2wavefronts[0] = NULL;
wf_components->d2wavefronts[0] = NULL;
break;
case affine2p_matrix_I2:
wf_components->mwavefronts[0] = NULL;
wf_components->i1wavefronts[0] = NULL;
wf_components->d1wavefronts[0] = NULL;
wf_components->i2wavefronts[0] = wavefront;
wf_components->i2wavefronts[0]->offsets[0] = 0;
wf_components->i2wavefronts[0]->lo = 0;
wf_components->i2wavefronts[0]->hi = 0;
wf_components->d2wavefronts[0] = NULL;
break;
case affine2p_matrix_D1:
wf_components->mwavefronts[0] = NULL;
wf_components->i1wavefronts[0] = NULL;
wf_components->d1wavefronts[0] = wavefront;
wf_components->d1wavefronts[0]->offsets[0] = 0;
wf_components->d1wavefronts[0]->lo = 0;
wf_components->d1wavefronts[0]->hi = 0;
// Nullify unused WFs
if (distance_metric==gap_affine) return;
wf_components->i2wavefronts[0] = NULL;
wf_components->d2wavefronts[0] = NULL;
break;
case affine2p_matrix_D2:
wf_components->mwavefronts[0] = NULL;
wf_components->i1wavefronts[0] = NULL;
wf_components->d1wavefronts[0] = NULL;
wf_components->i2wavefronts[0] = NULL;
wf_components->d2wavefronts[0] = wavefront;
wf_components->d2wavefronts[0]->offsets[0] = 0;
wf_components->d2wavefronts[0]->lo = 0;
wf_components->d2wavefronts[0]->hi = 0;
break;
default:
break;
}
}
}
void wavefront_unialign_init(
wavefront_aligner_t* const wf_aligner,
const char* const pattern,
const int pattern_length,
const char* const text,
const int text_length,
const affine2p_matrix_type component_begin,
const affine2p_matrix_type component_end) {
// Parameters
wavefront_align_status_t* const align_status = &wf_aligner->align_status;
// Resize wavefront aligner
wavefront_unialign_resize(wf_aligner,pattern,pattern_length,text,text_length,false);
// Configure WF-compute function
switch (wf_aligner->penalties.distance_metric) {
case indel:
case edit:
align_status->wf_align_compute = &wavefront_compute_edit;
break;
case gap_linear:
align_status->wf_align_compute = &wavefront_compute_linear;
break;
case gap_affine:
align_status->wf_align_compute = &wavefront_compute_affine;
break;
case gap_affine_2p:
align_status->wf_align_compute = &wavefront_compute_affine2p;
break;
default:
fprintf(stderr,"[WFA] Distance function not implemented\n");
exit(1);
break;
}
// Configure WF-extend function
const bool end2end = (wf_aligner->alignment_form.span == alignment_end2end);
if (wf_aligner->match_funct != NULL) {
align_status->wf_align_extend = &wavefront_extend_custom;
} else if (end2end) {
align_status->wf_align_extend = &wavefront_extend_end2end;
} else {
align_status->wf_align_extend = &wavefront_extend_endsfree;
}
// Initialize wavefront
wf_aligner->alignment_end_pos.score = -1; // Not aligned
wf_aligner->alignment_end_pos.k = DPMATRIX_DIAGONAL_NULL;
wf_aligner->component_begin = component_begin;
wf_aligner->component_end = component_end;
wavefront_unialign_initialize_wavefronts(wf_aligner,pattern_length,text_length);
// Plot (WF_0)
if (wf_aligner->plot != NULL) wavefront_plot(wf_aligner,0,0);
}
/*
* Limits
*/
bool wavefront_unialign_reached_limits(
wavefront_aligner_t* const wf_aligner,
const int score) {
// Check alignment-score limit
if (score >= wf_aligner->system.max_alignment_score) {
wf_aligner->cigar->score = wf_aligner->system.max_alignment_score;
wf_aligner->align_status.status = WF_STATUS_MAX_SCORE_REACHED;
wf_aligner->align_status.score = score;
return true; // Stop
}
// Global probing interval
alignment_system_t* const system = &wf_aligner->system;
if (score % system->probe_interval_global != 0) return false; // Continue
if (system->verbose >= 3) {
wavefront_unialign_print_status(stderr,wf_aligner,score); // DEBUG
}
// BT-Buffer
wavefront_components_t* const wf_components = &wf_aligner->wf_components;
if (wf_components->bt_buffer!=NULL && (score%system->probe_interval_compact)==0) {
uint64_t bt_memory = wf_backtrace_buffer_get_size_used(wf_components->bt_buffer);
// Check BT-buffer memory
if (bt_memory > system->max_memory_compact) {
// Compact BT-buffer
wavefront_components_compact_bt_buffer(wf_components,score,wf_aligner->system.verbose);
// Set new buffer limit
bt_memory = wf_backtrace_buffer_get_size_used(wf_components->bt_buffer);
uint64_t proposed_mem = (double)bt_memory * TELESCOPIC_FACTOR;
if (system->max_memory_compact < proposed_mem && proposed_mem < system->max_memory_abort) {
proposed_mem = system->max_memory_compact;
}
// Reset (if maximum compacts has been performed)
if (wf_components->bt_buffer->num_compactions >= system->max_partial_compacts) {
wf_backtrace_buffer_reset_compaction(wf_components->bt_buffer);
}
}
}
// Check overall memory used
const uint64_t wf_memory_used = wavefront_aligner_get_size(wf_aligner);
if (wf_memory_used > system->max_memory_abort) {
wf_aligner->align_status.status = WF_STATUS_OOM;
wf_aligner->align_status.score = score;
return true; // Stop
}
// Otherwise continue
return false;
}
/*
* Terminate alignment (backtrace)
*/
void wavefront_unialign_terminate(
wavefront_aligner_t* const wf_aligner,
const int score) {
// Parameters
const int pattern_length = wf_aligner->pattern_length;
const int text_length = wf_aligner->text_length;
// Retrieve alignment
if (wf_aligner->alignment_scope == compute_score) {
cigar_clear(wf_aligner->cigar);
wf_aligner->cigar->score =
wavefront_compute_classic_score(wf_aligner,pattern_length,text_length,score);
} else {
// Parameters
wavefront_components_t* const wf_components = &wf_aligner->wf_components;
const int alignment_end_k = wf_aligner->alignment_end_pos.k;
const wf_offset_t alignment_end_offset = wf_aligner->alignment_end_pos.offset;
if (wf_components->bt_piggyback) {
// Fetch wavefront
const bool memory_modular = wf_aligner->wf_components.memory_modular;
const int max_score_scope = wf_aligner->wf_components.max_score_scope;
const int score_mod = (memory_modular) ? score % max_score_scope : score;
wavefront_t* const mwavefront = wf_components->mwavefronts[score_mod];
// Backtrace alignment from buffer (unpacking pcigar)
wavefront_backtrace_pcigar(
wf_aligner,alignment_end_k,alignment_end_offset,
mwavefront->bt_pcigar[alignment_end_k],
mwavefront->bt_prev[alignment_end_k]);
} else {
// Backtrace alignment
if (wf_aligner->penalties.distance_metric <= gap_linear) {
wavefront_backtrace_linear(wf_aligner,
score,alignment_end_k,alignment_end_offset);
} else {
wavefront_backtrace_affine(wf_aligner,
wf_aligner->component_begin,wf_aligner->component_end,
score,alignment_end_k,alignment_end_offset);
}
}
// Set score & finish
wf_aligner->cigar->score =
wavefront_compute_classic_score(wf_aligner,pattern_length,text_length,score);
}
// Set successful
wf_aligner->align_status.status = WF_STATUS_SUCCESSFUL;
}
/*
* Classic WF-Alignment (Unidirectional)
*/
int wavefront_unialign(
wavefront_aligner_t* const wf_aligner) {
// Parameters
wavefront_align_status_t* const align_status = &wf_aligner->align_status;
void (*wf_align_compute)(wavefront_aligner_t* const,const int) = align_status->wf_align_compute;
int (*wf_align_extend)(wavefront_aligner_t* const,const int) = align_status->wf_align_extend;
// Compute wavefronts of increasing score
align_status->num_null_steps = 0;
int score = align_status->score;
while (true) {
// Exact extend s-wavefront
const int finished = (*wf_align_extend)(wf_aligner,score);
if (finished) {
// DEBUG
// wavefront_aligner_print(stderr,wf_aligner,0,score,7,0);
if (align_status->status == WF_STATUS_END_REACHED) {
wavefront_unialign_terminate(wf_aligner,score);
}
return align_status->status;
}
// Compute (s+1)-wavefront
++score;
(*wf_align_compute)(wf_aligner,score);
// Probe limits
if (wavefront_unialign_reached_limits(wf_aligner,score)) return align_status->status;
// Plot
if (wf_aligner->plot != NULL) wavefront_plot(wf_aligner,score,0);
// DEBUG
//wavefront_aligner_print(stderr,wf_aligner,score,score,7,0);
}
// Return OK
align_status->score = score;
align_status->status = WF_STATUS_SUCCESSFUL;
return WF_STATUS_SUCCESSFUL;
}
/*
* Display
*/
void wavefront_unialign_print_status(
FILE* const stream,
wavefront_aligner_t* const wf_aligner,
const int score) {
// Parameters
wavefront_components_t* const wf_components = &wf_aligner->wf_components;
// Approximate progress
const int dist_total = MAX(wf_aligner->text_length,wf_aligner->pattern_length);
int s = (wf_components->memory_modular) ? score%wf_components->max_score_scope : score;
wavefront_t* wavefront = wf_components->mwavefronts[s];
if (wavefront==NULL && s>0) {
s = (wf_components->memory_modular) ? (score-1)%wf_components->max_score_scope : (score-1);
wavefront = wf_components->mwavefronts[s];
}
int dist_max = -1, wf_len = -1, k;
if (wavefront!=NULL) {
wf_offset_t* const offsets = wavefront->offsets;
for (k=wavefront->lo;k<=wavefront->hi;++k) {
const int dist = MAX(WAVEFRONT_V(k,offsets[k]),WAVEFRONT_H(k,offsets[k]));
dist_max = MAX(dist_max,dist);
}
wf_len = wavefront->hi-wavefront->lo+1;
}
// Memory used
const uint64_t slab_size = wavefront_slab_get_size(wf_aligner->wavefront_slab);
const uint64_t bt_buffer_used = (wf_components->bt_buffer) ?
wf_backtrace_buffer_get_size_used(wf_components->bt_buffer) : 0;
// Progress
const float aligned_progress = (dist_max>=0) ? (100.0f*(float)dist_max/(float)dist_total) : -1.0f;
const float million_offsets = (wf_len>=0) ? (float)wf_len/1000000.0f : -1.0f;
// Print one-line status
fprintf(stream,"[");
wavefront_aligner_print_type(stream,wf_aligner);
fprintf(stream,
"] SequenceLength=(%d,%d) Score %d (~ %2.3f%% aligned). "
"MemoryUsed(WF-Slab,BT-buffer)=(%lu MB,%lu MB). "
"Wavefronts ~ %2.3f Moffsets\n",
wf_aligner->pattern_length,wf_aligner->text_length,score,aligned_progress,
CONVERT_B_TO_MB(slab_size),CONVERT_B_TO_MB(bt_buffer_used),million_offsets);
}
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