File: gtk_timeout.py

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matplotlib 1.1.1~rc2-1
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import gobject
import numpy as np
import matplotlib
matplotlib.use('GTKAgg')

import matplotlib.pyplot as plt

fig = plt.figure()
ax = fig.add_subplot(111)
line, = ax.plot(np.random.rand(10))
ax.set_ylim(0, 1)

def update():
    line.set_ydata(np.random.rand(10))
    fig.canvas.draw_idle()
    return True  # return False to terminate the updates

gobject.timeout_add(100, update)  # you can also use idle_add to update when gtk is idle
plt.show()