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#!/usr/bin/perl
# This library is free software; you can redistribute it and/or
# modify it under the terms of the GNU Lesser General Public
# License as published by the Free Software Foundation; either
# version 2.1 of the License, or (at your option) any later version.
#
# This library is distributed in the hope that it will be useful,
# but WITHOUT ANY WARRANTY; without even the implied warranty of
# MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
# Lesser General Public License for more details.
#
# You should have received a copy of the GNU Lesser General Public
# License along with this library ('COPYING'); if not, write to the Free Software
# Foundation, Inc., 51 Franklin St, Fifth Floor, Boston, MA 02110-1301 USA
use strict;
use warnings;
use XML::Twig;
=head1 NAME
mipe2pcroverview.pl - Generates overview of PCRs from a MIPE file
included in output: PCR ID, projects, researchers, number of SNPs, length of PCR fragment, remarks
based on MIPE version v1.1
arguments: * mipe_file
* (optional) list of PCR IDs
=head1 SYNOPSIS
mipe2pcroverview.pl your_file.mipe <pcr_id1> <pcr_id2>
=head1 ADDITIONAL INFO
See http://mipe.sourceforge.net
=head1 AUTHOR
Jan Aerts (jan.aerts@bbsrc.ac.uk)
=cut
my ( $file, @pcr_ids ) = @ARGV;
if ( not defined $file ) { die "Please provide filename\n" };
my $twig = XML::Twig->new( TwigHandlers => { pcr => \&pcr }
, pretty_print => 'indented' );
$twig->parsefile($file);
exit;
sub pcr {
my ( $twig, $pcr ) = @_;
my $to_include = 0;
my $pcr_id = $pcr->{att}->{id};
if ( scalar @pcr_ids > 0 ) {
$to_include = 0;
foreach ( @pcr_ids ) {
if ( $pcr_id =~ /$_/i ) {
$to_include = 1;
}
}
} else {
$to_include = 1;
}
if ( $to_include ) {
my @researchers = $pcr->children('researcher');
my $researchers;
if ( scalar @researchers > 0 ) {
foreach ( @researchers ) {
$researchers .= $_->text . ';';
}
chop $researchers;
} else {
$researchers = 'NONE';
}
my @projects = $pcr->children('project');
my $projects;
if ( scalar @projects > 0 ) {
foreach ( @projects ) {
$projects .= $_->text . ';';
}
chop $projects;
} else {
$projects = 'NONE';
}
my $length = 'UNKNOWN';
if ( defined $pcr->next_elt('design')->first_child('seq') ) {
$length = length $pcr->next_elt('design')->first_child('seq')->text;
} elsif ( defined $pcr->next_elt('design')->first_child('pos') ) {
my @range = split /\-/, $pcr->next_elt('design')->first_child('pos')->text;
$length = $range[1] - $range[0];
}
my $remarks;
my @remarks = $pcr->children('remark');
if ( scalar @remarks == 0 ) {
$remarks = 'NO REMARK';
} else {
foreach ( @remarks ) {
$remarks .= $_->text . '; ';
}
chop $remarks; chop $remarks;
}
my @snps = ( defined $pcr->first_child('use') ) ? $pcr->first_child('use')->children('snp') : ();
print $pcr_id, "\t", $projects, "\t", $researchers, "\t", scalar @snps, " SNPs\t", $length, "bp\t", $remarks, "\n";
}
}
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