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/*
* bellerophon.cpp
* Mothur
*
* Created by Sarah Westcott on 7/9/09.
* Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
*
*/
#include "bellerophon.h"
#include "eachgapdist.h"
#include "ignoregaps.h"
#include "onegapdist.h"
/***************************************************************************************************************/
Bellerophon::Bellerophon(string name, bool filterSeqs, bool c, int win, int inc, int p, string o) : Chimera() {
try {
fastafile = name;
correction = c;
outputDir = o;
window = win;
increment = inc;
processors = p;
//read in sequences
seqs = readSeqs(fastafile);
numSeqs = seqs.size();
if (numSeqs == 0) { m->mothurOut("Error in reading you sequences."); m->mothurOutEndLine(); exit(1); }
//do soft filter
if (filterSeqs) {
createFilter(seqs, 0.5);
for (int i = 0; i < seqs.size(); i++) { runFilter(seqs[i]); }
}
distCalculator = new eachGapDist();
//set default window to 25% of sequence length
string seq0 = seqs[0]->getAligned();
if (window == 0) { window = seq0.length() / 4; }
else if (window > (seq0.length() / 2)) {
m->mothurOut("Your sequence length is = " + toString(seq0.length()) + ". You have selected a window size greater than the length of half your aligned sequence. I will run it with a window size of " + toString((seq0.length() / 2))); m->mothurOutEndLine();
window = (seq0.length() / 2);
}
if (increment > (seqs[0]->getAlignLength() - (2*window))) {
if (increment != 10) {
m->mothurOut("You have selected a increment that is too large. I will use the default."); m->mothurOutEndLine();
increment = 10;
if (increment > (seqs[0]->getAlignLength() - (2*window))) { increment = 0; }
}else{ increment = 0; }
}
if (increment == 0) { iters = 1; }
else { iters = ((seqs[0]->getAlignLength() - (2*window)) / increment); }
//initialize pref
pref.resize(iters);
for (int i = 0; i < iters; i++) {
Preference temp;
for (int j = 0; j < numSeqs; j++) {
pref[i].push_back(temp);
}
}
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "Bellerophon");
exit(1);
}
}
//***************************************************************************************************************
int Bellerophon::print(ostream& out, ostream& outAcc, string s) {
try {
int above1 = 0;
//sorted "best" preference scores for all seqs
vector<Preference> best = getBestPref();
if (m->control_pressed) { return numSeqs; }
out << "Name\tScore\tLeft\tRight\t" << endl;
//output prefenence structure to .chimeras file
for (int i = 0; i < best.size(); i++) {
if (m->control_pressed) { return numSeqs; }
out << best[i].name << '\t' << setprecision(3) << best[i].score << '\t' << best[i].leftParent << '\t' << best[i].rightParent << endl;
//calc # of seqs with preference above 1.0
if (best[i].score > 1.0) {
above1++;
outAcc << best[i].name << endl;
m->mothurOut(best[i].name + " is a suspected chimera at breakpoint " + toString(best[i].midpoint)); m->mothurOutEndLine();
m->mothurOut("It's score is " + toString(best[i].score) + " with suspected left parent " + best[i].leftParent + " and right parent " + best[i].rightParent); m->mothurOutEndLine();
}
}
//output results to screen
m->mothurOutEndLine();
m->mothurOut("Sequence with preference score above 1.0: " + toString(above1)); m->mothurOutEndLine();
int spot;
spot = best.size()-1;
m->mothurOut("Minimum:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.975;
m->mothurOut("2.5%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.75;
m->mothurOut("25%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.50;
m->mothurOut("Median: \t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.25;
m->mothurOut("75%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.025;
m->mothurOut("97.5%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = 0;
m->mothurOut("Maximum:\t" + toString(best[spot].score)); m->mothurOutEndLine();
return numSeqs;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "print");
exit(1);
}
}
#ifdef USE_MPI
//***************************************************************************************************************
int Bellerophon::print(MPI_File& out, MPI_File& outAcc, string s) {
try {
int pid;
MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
if (pid == 0) {
string outString = "";
//sorted "best" preference scores for all seqs
vector<Preference> best = getBestPref();
int above1 = 0;
int ninetyfive = best.size() * 0.05;
float cutoffScore = best[ninetyfive].score;
if (m->control_pressed) { return numSeqs; }
outString = "Name\tScore\tLeft\tRight\n";
MPI_Status status;
int olength = outString.length();
char* buf5 = new char[olength];
memcpy(buf5, outString.c_str(), olength);
MPI_File_write_shared(out, buf5, olength, MPI_CHAR, &status);
delete buf5;
//output prefenence structure to .chimeras file
for (int i = 0; i < best.size(); i++) {
if (m->control_pressed) { return numSeqs; }
outString = best[i].name + "\t" + toString(best[i].score) + "\t" + best[i].leftParent + "\t" + best[i].rightParent + "\n";
MPI_Status status;
int length = outString.length();
char* buf2 = new char[length];
memcpy(buf2, outString.c_str(), length);
MPI_File_write_shared(out, buf2, length, MPI_CHAR, &status);
delete buf2;
//calc # of seqs with preference above 95%tile
if (best[i].score >= cutoffScore) {
above1++;
string outAccString = "";
outAccString += best[i].name + "\n";
MPI_Status statusAcc;
length = outAccString.length();
char* buf = new char[length];
memcpy(buf, outAccString.c_str(), length);
MPI_File_write_shared(outAcc, buf, length, MPI_CHAR, &statusAcc);
delete buf;
cout << best[i].name << " is a suspected chimera at breakpoint " << toString(best[i].midpoint) << endl;
cout << "It's score is " << toString(best[i].score) << " with suspected left parent " << best[i].leftParent << " and right parent " << best[i].rightParent << endl;
}
}
//output results to screen
m->mothurOutEndLine();
m->mothurOut("Sequence with preference score above " + toString(cutoffScore) + ": " + toString(above1)); m->mothurOutEndLine();
int spot;
spot = best.size()-1;
m->mothurOut("Minimum:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.975;
m->mothurOut("2.5%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.75;
m->mothurOut("25%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.50;
m->mothurOut("Median: \t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.25;
m->mothurOut("75%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = best.size() * 0.025;
m->mothurOut("97.5%-tile:\t" + toString(best[spot].score)); m->mothurOutEndLine();
spot = 0;
m->mothurOut("Maximum:\t" + toString(best[spot].score)); m->mothurOutEndLine();
}
return numSeqs;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "print");
exit(1);
}
}
#endif
//********************************************************************************************************************
//sorts highest score to lowest
inline bool comparePref(Preference left, Preference right){
return (left.score > right.score);
}
//***************************************************************************************************************
int Bellerophon::getChimeras() {
try {
//create breaking points
vector<int> midpoints; midpoints.resize(iters, window);
for (int i = 1; i < iters; i++) { midpoints[i] = midpoints[i-1] + increment; }
#ifdef USE_MPI
int pid, numSeqsPerProcessor;
MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
MPI_Comm_size(MPI_COMM_WORLD, &processors);
numSeqsPerProcessor = iters / processors;
//each process hits this only once
unsigned long long startPos = pid * numSeqsPerProcessor;
if(pid == processors - 1){
numSeqsPerProcessor = iters - pid * numSeqsPerProcessor;
}
lines.push_back(linePair(startPos, numSeqsPerProcessor));
//fill pref with scores
driverChimeras(midpoints, lines[0]);
if (m->control_pressed) { return 0; }
//each process must send its parts back to pid 0
if (pid == 0) {
//receive results
for (int j = 1; j < processors; j++) {
vector<string> MPIBestSend;
for (int i = 0; i < numSeqs; i++) {
if (m->control_pressed) { return 0; }
MPI_Status status;
//receive string
int length;
MPI_Recv(&length, 1, MPI_INT, j, 2001, MPI_COMM_WORLD, &status);
char* buf = new char[length];
MPI_Recv(&buf[0], length, MPI_CHAR, j, 2001, MPI_COMM_WORLD, &status);
string temp = buf;
if (temp.length() > length) { temp = temp.substr(0, length); }
delete buf;
MPIBestSend.push_back(temp);
}
fillPref(j, MPIBestSend);
if (m->control_pressed) { return 0; }
}
}else {
//takes best window for each sequence and turns Preference to string that can be parsed by pid 0.
//played with this a bit, but it may be better to try user-defined datatypes with set string lengths??
vector<string> MPIBestSend = getBestWindow(lines[0]);
pref.clear();
//send your result to parent
for (int i = 0; i < numSeqs; i++) {
if (m->control_pressed) { return 0; }
int bestLength = MPIBestSend[i].length();
char* buf = new char[bestLength];
memcpy(buf, MPIBestSend[i].c_str(), bestLength);
MPI_Send(&bestLength, 1, MPI_INT, 0, 2001, MPI_COMM_WORLD);
MPI_Send(buf, bestLength, MPI_CHAR, 0, 2001, MPI_COMM_WORLD);
delete buf;
}
MPIBestSend.clear();
}
MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
#else
//divide breakpoints between processors
#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
if(processors == 1){
lines.push_back(linePair(0, iters));
//fill pref with scores
driverChimeras(midpoints, lines[0]);
}else{
int numSeqsPerProcessor = iters / processors;
for (int i = 0; i < processors; i++) {
unsigned long long startPos = i * numSeqsPerProcessor;
if(i == processors - 1){
numSeqsPerProcessor = iters - i * numSeqsPerProcessor;
}
lines.push_back(linePair(startPos, numSeqsPerProcessor));
}
createProcesses(midpoints);
}
#else
lines.push_back(linePair(0, iters));
///fill pref with scores
driverChimeras(midpoints, lines[0]);
#endif
#endif
return 0;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "getChimeras");
exit(1);
}
}
/**************************************************************************************************/
int Bellerophon::createProcesses(vector<int> mid) {
try {
#if defined (__APPLE__) || (__MACH__) || (linux) || (__linux) || (__linux__) || (__unix__) || (__unix)
int process = 0;
int exitCommand = 1;
vector<int> processIDS;
//loop through and create all the processes you want
while (process != processors) {
int pid = fork();
if (pid > 0) {
processIDS.push_back(pid); //create map from line number to pid so you can append files in correct order later
process++;
}else if (pid == 0){
exitCommand = driverChimeras(mid, lines[process]);
string tempOut = outputDir + toString(getpid()) + ".temp";
writePrefs(tempOut, lines[process]);
exit(0);
}else {
m->mothurOut("[ERROR]: unable to spawn the necessary processes."); m->mothurOutEndLine();
for (int i = 0; i < processIDS.size(); i++) { kill (processIDS[i], SIGINT); }
exit(0);
}
}
//force parent to wait until all the processes are done
for (int i=0;i<processors;i++) {
int temp = processIDS[i];
wait(&temp);
}
//get info that other processes created
for (int i = 0; i < processIDS.size(); i++) {
string tempIn = outputDir + toString(processIDS[i]) + ".temp";
readPrefs(tempIn);
}
return exitCommand;
#endif
}
catch(exception& e) {
m->errorOut(e, "AlignCommand", "createProcesses");
exit(1);
}
}
//***************************************************************************************************************
int Bellerophon::driverChimeras(vector<int> midpoints, linePair line) {
try {
for (int h = line.start; h < (line.start + line.num); h++) {
count = h;
int midpoint = midpoints[h];
//initialize pref[count]
for (int i = 0; i < numSeqs; i++ ) {
pref[count][i].name = seqs[i]->getName();
pref[count][i].midpoint = midpoint;
}
if (m->control_pressed) { return 0; }
//create 2 vectors of sequences, 1 for left side and one for right side
vector<Sequence> left; vector<Sequence> right;
for (int i = 0; i < seqs.size(); i++) {
if (m->control_pressed) { return 0; }
//cout << "midpoint = " << midpoint << "\twindow = " << window << endl;
//cout << "whole = " << seqs[i]->getAligned().length() << endl;
//save left side
string seqLeft = seqs[i]->getAligned().substr(midpoint-window, window);
Sequence tempLeft;
tempLeft.setName(seqs[i]->getName());
tempLeft.setAligned(seqLeft);
left.push_back(tempLeft);
//cout << "left = " << tempLeft.getAligned().length() << endl;
//save right side
string seqRight = seqs[i]->getAligned().substr(midpoint, window);
Sequence tempRight;
tempRight.setName(seqs[i]->getName());
tempRight.setAligned(seqRight);
right.push_back(tempRight);
//cout << "right = " << seqRight.length() << endl;
}
//this should be parallelized
//perference = sum of (| distance of my left to sequence j's left - distance of my right to sequence j's right | )
//create a matrix containing the distance from left to left and right to right
//calculate distances
SparseMatrix* SparseLeft = new SparseMatrix();
SparseMatrix* SparseRight = new SparseMatrix();
createSparseMatrix(0, left.size(), SparseLeft, left);
if (m->control_pressed) { delete SparseLeft; delete SparseRight; return 0; }
createSparseMatrix(0, right.size(), SparseRight, right);
if (m->control_pressed) { delete SparseLeft; delete SparseRight; return 0; }
left.clear(); right.clear();
vector<SeqMap> distMapRight;
vector<SeqMap> distMapLeft;
// Create a data structure to quickly access the distance information.
//this is from thallingers reimplementation on get.oturep
// It consists of a vector of distance maps, where each map contains
// all distances of a certain sequence. Vector and maps are accessed
// via the index of a sequence in the distance matrix
distMapRight = vector<SeqMap>(numSeqs);
distMapLeft = vector<SeqMap>(numSeqs);
//cout << "left" << endl << endl;
for (MatData currentCell = SparseLeft->begin(); currentCell != SparseLeft->end(); currentCell++) {
distMapLeft[currentCell->row][currentCell->column] = currentCell->dist;
if (m->control_pressed) { delete SparseLeft; delete SparseRight; return 0; }
//cout << " i = " << currentCell->row << " j = " << currentCell->column << " dist = " << currentCell->dist << endl;
}
//cout << "right" << endl << endl;
for (MatData currentCell = SparseRight->begin(); currentCell != SparseRight->end(); currentCell++) {
distMapRight[currentCell->row][currentCell->column] = currentCell->dist;
if (m->control_pressed) { delete SparseLeft; delete SparseRight; return 0; }
//cout << " i = " << currentCell->row << " j = " << currentCell->column << " dist = " << currentCell->dist << endl;
}
delete SparseLeft;
delete SparseRight;
//fill preference structure
generatePreferences(distMapLeft, distMapRight, midpoint);
if (m->control_pressed) { return 0; }
//report progress
if((h+1) % 10 == 0){ cout << "Processing sliding window: " << toString(h+1) << "\n"; m->mothurOutJustToLog("Processing sliding window: " + toString(h+1) + "\n") ; }
}
//report progress
if((line.start + line.num) % 10 != 0){ cout << "Processing sliding window: " << toString(line.start + line.num) << "\n"; m->mothurOutJustToLog("Processing sliding window: " + toString(line.start + line.num) + "\n") ; }
return 0;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "driverChimeras");
exit(1);
}
}
/***************************************************************************************************************/
int Bellerophon::createSparseMatrix(int startSeq, int endSeq, SparseMatrix* sparse, vector<Sequence> s){
try {
for(int i=startSeq; i<endSeq; i++){
for(int j=0;j<i;j++){
if (m->control_pressed) { return 0; }
distCalculator->calcDist(s[i], s[j]);
float dist = distCalculator->getDist();
PCell temp(i, j, dist);
sparse->addCell(temp);
}
}
return 1;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "createSparseMatrix");
exit(1);
}
}
/***************************************************************************************************************/
int Bellerophon::generatePreferences(vector<SeqMap> left, vector<SeqMap> right, int mid){
try {
SeqMap::iterator itR;
SeqMap::iterator itL;
for (int i = 0; i < left.size(); i++) {
SeqMap currentLeft = left[i]; //example i = 3; currentLeft is a map of 0 to the distance of sequence 3 to sequence 0,
// 1 to the distance of sequence 3 to sequence 1,
// 2 to the distance of sequence 3 to sequence 2.
SeqMap currentRight = right[i]; // same as left but with distances on the right side.
for (int j = 0; j < i; j++) {
if (m->control_pressed) { return 0; }
itL = currentLeft.find(j);
itR = currentRight.find(j);
//cout << " i = " << i << " j = " << j << " distLeft = " << itL->second << endl;
//cout << " i = " << i << " j = " << j << " distright = " << itR->second << endl;
//if you can find this entry update the preferences
if ((itL != currentLeft.end()) && (itR != currentRight.end())) {
if (!correction) {
pref[count][i].score += abs((itL->second - itR->second));
pref[count][j].score += abs((itL->second - itR->second));
//cout << "left " << i << " " << j << " = " << itL->second << " right " << i << " " << j << " = " << itR->second << endl;
//cout << "abs = " << abs((itL->second - itR->second)) << endl;
//cout << i << " score = " << pref[i].score[1] << endl;
//cout << j << " score = " << pref[j].score[1] << endl;
}else {
pref[count][i].score += abs((sqrt(itL->second) - sqrt(itR->second)));
pref[count][j].score += abs((sqrt(itL->second) - sqrt(itR->second)));
//cout << "left " << i << " " << j << " = " << itL->second << " right " << i << " " << j << " = " << itR->second << endl;
//cout << "abs = " << abs((sqrt(itL->second) - sqrt(itR->second))) << endl;
//cout << i << " score = " << pref[i].score[1] << endl;
//cout << j << " score = " << pref[j].score[1] << endl;
}
//cout << "pref[" << i << "].closestLeft[1] = " << pref[i].closestLeft[1] << " parent = " << pref[i].leftParent[1] << endl;
//are you the closest left sequence
if (itL->second < pref[count][i].closestLeft) {
pref[count][i].closestLeft = itL->second;
pref[count][i].leftParent = seqs[j]->getName();
//cout << "updating closest left to " << pref[i].leftParent[1] << endl;
}
//cout << "pref[" << j << "].closestLeft[1] = " << pref[j].closestLeft[1] << " parent = " << pref[j].leftParent[1] << endl;
if (itL->second < pref[count][j].closestLeft) {
pref[count][j].closestLeft = itL->second;
pref[count][j].leftParent = seqs[i]->getName();
//cout << "updating closest left to " << pref[j].leftParent[1] << endl;
}
//are you the closest right sequence
if (itR->second < pref[count][i].closestRight) {
pref[count][i].closestRight = itR->second;
pref[count][i].rightParent = seqs[j]->getName();
}
if (itR->second < pref[count][j].closestRight) {
pref[count][j].closestRight = itR->second;
pref[count][j].rightParent = seqs[i]->getName();
}
}
}
}
return 1;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "generatePreferences");
exit(1);
}
}
/**************************************************************************************************/
vector<Preference> Bellerophon::getBestPref() {
try {
vector<Preference> best;
//for each sequence
for (int i = 0; i < numSeqs; i++) {
//set best pref score to first one
Preference temp = pref[0][i];
if (m->control_pressed) { return best; }
//for each window
for (int j = 1; j < pref.size(); j++) {
//is this a better score
if (pref[j][i].score > temp.score) { temp = pref[j][i]; }
}
best.push_back(temp);
}
//rank preference score to eachother
float dme = 0.0;
float expectedPercent = 1 / (float) (best.size());
for (int i = 0; i < best.size(); i++) { dme += best[i].score; }
for (int i = 0; i < best.size(); i++) {
if (m->control_pressed) { return best; }
//gives the actual percentage of the dme this seq adds
best[i].score = best[i].score / dme;
//how much higher or lower is this than expected
best[i].score = best[i].score / expectedPercent;
}
//sort Preferences highest to lowest
sort(best.begin(), best.end(), comparePref);
return best;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "getBestPref");
exit(1);
}
}
/**************************************************************************************************/
int Bellerophon::writePrefs(string file, linePair tempLine) {
try {
ofstream outTemp;
m->openOutputFile(file, outTemp);
//lets you know what part of the pref matrix you are writing
outTemp << tempLine.start << '\t' << tempLine.num << endl;
for (int i = tempLine.start; i < (tempLine.start + tempLine.num); i++) {
for (int j = 0; j < numSeqs; j++) {
if (m->control_pressed) { outTemp.close(); m->mothurRemove(file); return 0; }
outTemp << pref[i][j].name << '\t' << pref[i][j].leftParent << '\t' << pref[i][j].rightParent << '\t';
outTemp << pref[i][j].score << '\t' << pref[i][j].closestLeft << '\t' << pref[i][j].closestRight << '\t' << pref[i][j].midpoint << endl;
}
}
outTemp.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "writePrefs");
exit(1);
}
}
/**************************************************************************************************/
int Bellerophon::readPrefs(string file) {
try {
ifstream inTemp;
m->openInputFile(file, inTemp);
int start, num;
//lets you know what part of the pref matrix you are writing
inTemp >> start >> num; m->gobble(inTemp);
for (int i = start; i < num; i++) {
for (int j = 0; j < numSeqs; j++) {
if (m->control_pressed) { inTemp.close(); m->mothurRemove(file); return 0; }
inTemp >> pref[i][j].name >> pref[i][j].leftParent >> pref[i][j].rightParent;
inTemp >> pref[i][j].score >> pref[i][j].closestLeft >> pref[i][j].closestRight >> pref[i][j].midpoint;
m->gobble(inTemp);
}
}
inTemp.close();
m->mothurRemove(file);
return 0;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "writePrefs");
exit(1);
}
}
/**************************************************************************************************/
vector<string> Bellerophon::getBestWindow(linePair line) {
try {
vector<string> best;
//for each sequence
for (int i = 0; i < numSeqs; i++) {
//set best pref score to first one
Preference temp = pref[line.start][i];
if (m->control_pressed) { return best; }
//for each window
for (int j = (line.start+1); j < (line.start+line.num); j++) {
//is this a better score
if (pref[j][i].score > temp.score) { temp = pref[j][i]; }
}
string tempString = temp.name + '\t' + temp.leftParent + '\t' + temp.rightParent + '\t' + toString(temp.score);
best.push_back(tempString);
}
return best;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "getBestWindow");
exit(1);
}
}
/**************************************************************************************************/
int Bellerophon::fillPref(int process, vector<string>& best) {
try {
//figure out where you start so you can put the best scores there
int numSeqsPerProcessor = iters / processors;
int start = process * numSeqsPerProcessor;
for (int i = 0; i < best.size(); i++) {
if (m->control_pressed) { return 0; }
istringstream iss (best[i],istringstream::in);
string tempScore;
iss >> pref[start][i].name >> pref[start][i].leftParent >> pref[start][i].rightParent >> tempScore;
convert(tempScore, pref[start][i].score);
}
return 0;
}
catch(exception& e) {
m->errorOut(e, "Bellerophon", "fillPref");
exit(1);
}
}
/**************************************************************************************************/
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