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/*
* binsequencecommand.cpp
* Mothur
*
* Created by Sarah Westcott on 4/3/09.
* Copyright 2009 Schloss Lab UMASS Amhers. All rights reserved.
*
*/
#include "binsequencecommand.h"
//**********************************************************************************************************************
vector<string> BinSeqCommand::setParameters(){
try {
CommandParameter plist("list", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(plist);
CommandParameter pfasta("fasta", "InputTypes", "", "", "none", "none", "none","fasta",false,true,true); parameters.push_back(pfasta);
CommandParameter pname("name", "InputTypes", "", "", "NameCount", "none", "none","",false,false,true); parameters.push_back(pname);
CommandParameter pcount("count", "InputTypes", "", "", "NameCount-CountGroup", "none", "none","",false,false,true); parameters.push_back(pcount);
CommandParameter pgroup("group", "InputTypes", "", "", "CountGroup", "none", "none","",false,false,true); parameters.push_back(pgroup);
CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string BinSeqCommand::getHelpString(){
try {
string helpString = "";
helpString += "The bin.seqs command parameters are list, fasta, name, count, label and group. The fasta and list are required, unless you have a valid current list and fasta file.\n";
helpString += "The label parameter allows you to select what distance levels you would like a output files created for, and are separated by dashes.\n";
helpString += "The bin.seqs command should be in the following format: bin.seqs(fasta=yourFastaFile, name=yourNamesFile, group=yourGroupFile, label=yourLabels).\n";
helpString += "Example bin.seqs(fasta=amazon.fasta, group=amazon.groups, name=amazon.names).\n";
helpString += "The default value for label is all lines in your inputfile.\n";
helpString += "The bin.seqs command outputs a .fasta file for each distance you specify appending the OTU number to each name.\n";
helpString += "If you provide a groupfile, then it also appends the sequences group to the name.\n";
helpString += "Note: No spaces between parameter labels (i.e. fasta), '=' and parameters (i.e.yourFastaFile).\n";
return helpString;
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string BinSeqCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "fasta") { pattern = "[filename],[distance],fasta"; } //makes file like: amazon.0.03.fasta
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true; }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "getOutputPattern");
exit(1);
}
}
//**********************************************************************************************************************
BinSeqCommand::BinSeqCommand(){
try {
abort = true; calledHelp = true;
setParameters();
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "BinSeqCommand");
exit(1);
}
}
//**********************************************************************************************************************
BinSeqCommand::BinSeqCommand(string option) {
try {
abort = false; calledHelp = false;
allLines = 1;
labels.clear();
//allow user to run help
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
vector<string> myArray = setParameters();
OptionParser parser(option);
map<string, string> parameters = parser.getParameters();
ValidParameters validParameter;
map<string, string>::iterator it;
//check to make sure all parameters are valid for command
for (it = parameters.begin(); it != parameters.end(); it++) {
if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
}
//initialize outputTypes
vector<string> tempOutNames;
outputTypes["fasta"] = tempOutNames;
//if the user changes the input directory command factory will send this info to us in the output parameter
string inputDir = validParameter.validFile(parameters, "inputdir", false);
if (inputDir == "not found"){ inputDir = ""; }
else {
string path;
it = parameters.find("fasta");
//user has given a template file
if(it != parameters.end()){
path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["fasta"] = inputDir + it->second; }
}
it = parameters.find("list");
//user has given a template file
if(it != parameters.end()){
path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["list"] = inputDir + it->second; }
}
it = parameters.find("name");
//user has given a template file
if(it != parameters.end()){
path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["name"] = inputDir + it->second; }
}
it = parameters.find("group");
//user has given a template file
if(it != parameters.end()){
path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["group"] = inputDir + it->second; }
}
it = parameters.find("count");
//user has given a template file
if(it != parameters.end()){
path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["count"] = inputDir + it->second; }
}
}
//check for required parameters
fastafile = validParameter.validFile(parameters, "fasta", true);
if (fastafile == "not found") { //if there is a current phylip file, use it
fastafile = m->getFastaFile();
if (fastafile != "") { m->mothurOut("Using " + fastafile + " as input file for the fasta parameter."); m->mothurOutEndLine(); }
else { m->mothurOut("You have no current fasta file and the fasta parameter is required."); m->mothurOutEndLine(); abort = true; }
}
else if (fastafile == "not open") { abort = true; }
else { m->setFastaFile(fastafile); }
listfile = validParameter.validFile(parameters, "list", true);
if (listfile == "not found") {
listfile = m->getListFile();
if (listfile != "") { m->mothurOut("Using " + listfile + " as input file for the list parameter."); m->mothurOutEndLine(); }
else { m->mothurOut("You have no current list file and the list parameter is required."); m->mothurOutEndLine(); abort = true; }
}
else if (listfile == "not open") { listfile = ""; abort = true; }
else { m->setListFile(listfile); }
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
outputDir = "";
outputDir += m->hasPath(listfile); //if user entered a file with a path then preserve it
}
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
label = validParameter.validFile(parameters, "label", false);
if (label == "not found") { label = ""; }
else {
if(label != "all") { m->splitAtDash(label, labels); allLines = 0; }
else { allLines = 1; }
}
namesfile = validParameter.validFile(parameters, "name", true);
if (namesfile == "not open") { namesfile = ""; abort = true; }
else if (namesfile == "not found") { namesfile = ""; }
else { m->setNameFile(namesfile); }
groupfile = validParameter.validFile(parameters, "group", true);
if (groupfile == "not open") { abort = true; }
else if (groupfile == "not found") { groupfile = ""; }
else { m->setGroupFile(groupfile); }
countfile = validParameter.validFile(parameters, "count", true);
if (countfile == "not open") { countfile = ""; abort = true; }
else if (countfile == "not found") { countfile = ""; }
else { m->setCountTableFile(countfile); }
if ((namesfile != "") && (countfile != "")) {
m->mothurOut("[ERROR]: you may only use one of the following: name or count."); m->mothurOutEndLine(); abort = true;
}
if ((groupfile != "") && (countfile != "")) {
m->mothurOut("[ERROR]: you may only use one of the following: group or count."); m->mothurOutEndLine(); abort=true;
}
if (countfile == "") {
if (namesfile == ""){
vector<string> files; files.push_back(fastafile);
parser.getNameFile(files);
}
}
}
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "BinSeqCommand");
exit(1);
}
}
//**********************************************************************************************************************
BinSeqCommand::~BinSeqCommand(){}
//**********************************************************************************************************************
int BinSeqCommand::execute(){
try {
if (abort == true) { if (calledHelp) { return 0; } return 2; }
int error = 0;
fasta = new FastaMap();
if (groupfile != "") {
groupMap = new GroupMap(groupfile);
groupMap->readMap();
}
//read fastafile
fasta->readFastaFile(fastafile);
//if user gave a namesfile then use it
if (namesfile != "") { readNamesFile(); }
if (countfile != "") { ct.readTable(countfile, true, false); }
input = new InputData(listfile, "list");
list = input->getListVector();
string lastLabel = list->getLabel();
if (m->control_pressed) { delete input; delete fasta; if (groupfile != "") { delete groupMap; } return 0; }
//if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
set<string> processedLabels;
set<string> userLabels = labels;
while((list != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
if(m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete fasta; if (groupfile != "") { delete groupMap; } return 0; }
if(allLines == 1 || labels.count(list->getLabel()) == 1){
error = process(list);
if (error == 1) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete fasta; if (groupfile != "") { delete groupMap; } return 0; }
processedLabels.insert(list->getLabel());
userLabels.erase(list->getLabel());
}
if ((m->anyLabelsToProcess(list->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
string saveLabel = list->getLabel();
delete list;
list = input->getListVector(lastLabel);
error = process(list);
if (error == 1) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete fasta; if (groupfile != "") { delete groupMap; } return 0; }
processedLabels.insert(list->getLabel());
userLabels.erase(list->getLabel());
//restore real lastlabel to save below
list->setLabel(saveLabel);
}
lastLabel = list->getLabel();
delete list;
list = input->getListVector();
}
if(m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete fasta; if (groupfile != "") { delete groupMap; } return 0; }
//output error messages about any remaining user labels
set<string>::iterator it;
bool needToRun = false;
for (it = userLabels.begin(); it != userLabels.end(); it++) {
m->mothurOut("Your file does not include the label " + *it);
if (processedLabels.count(lastLabel) != 1) {
m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
needToRun = true;
}else {
m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
}
}
//run last label if you need to
if (needToRun == true) {
if (list != NULL) { delete list; }
list = input->getListVector(lastLabel);
error = process(list);
if (error == 1) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } delete input; delete fasta; if (groupfile != "") { delete groupMap; } return 0; }
delete list;
}
delete input;
delete fasta;
if (groupfile != "") { delete groupMap; }
if(m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
//set align file as new current fastafile
string currentFasta = "";
itTypes = outputTypes.find("fasta");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { currentFasta = (itTypes->second)[0]; m->setFastaFile(currentFasta); }
}
m->mothurOutEndLine();
m->mothurOut("Output File Names: "); m->mothurOutEndLine();
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
void BinSeqCommand::readNamesFile() {
try {
vector<string> dupNames;
m->openInputFile(namesfile, inNames);
string name, names, sequence;
while(inNames){
inNames >> name; //read from first column A
inNames >> names; //read from second column A,B,C,D
dupNames.clear();
//parse names into vector
m->splitAtComma(names, dupNames);
//store names in fasta map
sequence = fasta->getSequence(name);
for (int i = 0; i < dupNames.size(); i++) {
fasta->push_back(dupNames[i], sequence);
}
m->gobble(inNames);
}
inNames.close();
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "readNamesFile");
exit(1);
}
}
//**********************************************************************************************************************
//return 1 if error, 0 otherwise
int BinSeqCommand::process(ListVector* list) {
try {
map<string, string> variables;
variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(listfile));
variables["[distance]"] = list->getLabel();
string outputFileName = getOutputFileName("fasta", variables);
m->openOutputFile(outputFileName, out);
outputNames.push_back(outputFileName); outputTypes["fasta"].push_back(outputFileName);
m->mothurOut(list->getLabel()); m->mothurOutEndLine();
//for each bin in the list vector
vector<string> binLabels = list->getLabels();
for (int i = 0; i < list->size(); i++) {
if (m->control_pressed) { return 1; }
string binnames = list->get(i);
vector<string> names;
m->splitAtComma(binnames, names);
for (int j = 0; j < names.size(); j++) {
string name = names[j];
//do work for that name
string sequence = fasta->getSequence(name);
if (countfile != "") {
if (sequence != "not found") {
if (ct.hasGroupInfo()) {
vector<string> groups = ct.getGroups(name);
string groupInfo = "";
for (int k = 0; k < groups.size()-1; k++) {
groupInfo += groups[k] + "-";
}
if (groups.size() != 0) { groupInfo += groups[groups.size()-1]; }
else { groupInfo = "not found"; }
name = name + "\t" + groupInfo + "\t" + binLabels[i] + "\tNumRep=" + toString(ct.getNumSeqs(name));
out << ">" << name << endl;
out << sequence << endl;
}else {
name = name + "\t" + binLabels[i] + "\tNumRep=" + toString(ct.getNumSeqs(name));
out << ">" << name << endl;
out << sequence << endl;
}
}else { m->mothurOut(name + " is missing from your fasta. Does your list file contain all sequence names or just the uniques?"); m->mothurOutEndLine(); return 1; }
}else {
if (sequence != "not found") {
//if you don't have groups
if (groupfile == "") {
name = name + "\t" + binLabels[i];
out << ">" << name << endl;
out << sequence << endl;
}else {//if you do have groups
string group = groupMap->getGroup(name);
if (group == "not found") {
m->mothurOut(name + " is missing from your group file. Please correct. "); m->mothurOutEndLine();
return 1;
}else{
name = name + "\t" + group + "\t" + binLabels[i];
out << ">" << name << endl;
out << sequence << endl;
}
}
}else { m->mothurOut(name + " is missing from your fasta or name file. Please correct. "); m->mothurOutEndLine(); return 1; }
}
}
}
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "BinSeqCommand", "process");
exit(1);
}
}
//**********************************************************************************************************************
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