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/*
* classify.cpp
* Mothur
*
* Created by westcott on 11/3/09.
* Copyright 2009 Schloss Lab. All rights reserved.
*
*/
#include "classify.h"
#include "sequence.hpp"
#include "kmerdb.hpp"
#include "suffixdb.hpp"
#include "blastdb.hpp"
#include "distancedb.hpp"
#include "referencedb.h"
/**************************************************************************************************/
void Classify::generateDatabaseAndNames(string tfile, string tempFile, string method, int kmerSize, float gapOpen, float gapExtend, float match, float misMatch) {
try {
ReferenceDB* rdb = ReferenceDB::getInstance();
if (tfile == "saved") { tfile = rdb->getSavedTaxonomy(); }
taxFile = tfile;
int numSeqs = 0;
if (tempFile == "saved") {
int start = time(NULL);
m->mothurOutEndLine(); m->mothurOut("Using sequences from " + rdb->getSavedReference() + " that are saved in memory."); m->mothurOutEndLine();
numSeqs = rdb->referenceSeqs.size();
templateFile = rdb->getSavedReference();
tempFile = rdb->getSavedReference();
bool needToGenerate = true;
string kmerDBName;
if(method == "kmer") {
database = new KmerDB(tempFile, kmerSize);
kmerDBName = tempFile.substr(0,tempFile.find_last_of(".")+1) + char('0'+ kmerSize) + "mer";
ifstream kmerFileTest(kmerDBName.c_str());
if(kmerFileTest){
bool GoodFile = m->checkReleaseVersion(kmerFileTest, m->getVersion());
if (GoodFile) { needToGenerate = false; }
}
}
else if(method == "suffix") { database = new SuffixDB(numSeqs); }
else if(method == "blast") { database = new BlastDB(tempFile.substr(0,tempFile.find_last_of(".")+1), gapOpen, gapExtend, match, misMatch, "", threadID); }
else if(method == "distance") { database = new DistanceDB(); }
else {
m->mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8.");
m->mothurOutEndLine();
database = new KmerDB(tempFile, 8);
}
if (needToGenerate) {
for (int k = 0; k < rdb->referenceSeqs.size(); k++) {
Sequence temp(rdb->referenceSeqs[k].getName(), rdb->referenceSeqs[k].getAligned());
names.push_back(temp.getName());
database->addSequence(temp);
}
if ((method == "kmer") && (!shortcuts)) {;} //don't print
else {database->generateDB(); }
}else if ((method == "kmer") && (!needToGenerate)) {
ifstream kmerFileTest(kmerDBName.c_str());
database->readKmerDB(kmerFileTest);
for (int k = 0; k < rdb->referenceSeqs.size(); k++) {
names.push_back(rdb->referenceSeqs[k].getName());
}
}
database->setNumSeqs(numSeqs);
m->mothurOut("It took " + toString(time(NULL) - start) + " to load " + toString(rdb->referenceSeqs.size()) + " sequences and generate the search databases.");m->mothurOutEndLine();
}else {
templateFile = tempFile;
int start = time(NULL);
m->mothurOut("Generating search database... "); cout.flush();
#ifdef USE_MPI
int pid, processors;
vector<unsigned long long> positions;
int tag = 2001;
MPI_Status status;
MPI_File inMPI;
MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
MPI_Comm_size(MPI_COMM_WORLD, &processors);
//char* inFileName = new char[tempFile.length()];
//memcpy(inFileName, tempFile.c_str(), tempFile.length());
char inFileName[1024];
strcpy(inFileName, tempFile.c_str());
MPI_File_open(MPI_COMM_WORLD, inFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer
//delete inFileName;
if (pid == 0) { //only one process needs to scan file
positions = m->setFilePosFasta(tempFile, numSeqs); //fills MPIPos, returns numSeqs
//send file positions to all processes
for(int i = 1; i < processors; i++) {
MPI_Send(&numSeqs, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
MPI_Send(&positions[0], (numSeqs+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
}
}else{
MPI_Recv(&numSeqs, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
positions.resize(numSeqs+1);
MPI_Recv(&positions[0], (numSeqs+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
}
//create database
if(method == "kmer") { database = new KmerDB(tempFile, kmerSize); }
else if(method == "suffix") { database = new SuffixDB(numSeqs); }
else if(method == "blast") { database = new BlastDB(tempFile.substr(0,tempFile.find_last_of(".")+1), gapOpen, gapExtend, match, misMatch, "", pid); }
else if(method == "distance") { database = new DistanceDB(); }
else {
m->mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8."); m->mothurOutEndLine();
database = new KmerDB(tempFile, 8);
}
//read file
for(int i=0;i<numSeqs;i++){
//read next sequence
int length = positions[i+1] - positions[i];
char* buf4 = new char[length];
MPI_File_read_at(inMPI, positions[i], buf4, length, MPI_CHAR, &status);
string tempBuf = buf4;
if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); }
delete buf4;
istringstream iss (tempBuf,istringstream::in);
Sequence temp(iss);
if (temp.getName() != "") {
if (rdb->save) { rdb->referenceSeqs.push_back(temp); }
names.push_back(temp.getName());
database->addSequence(temp);
}
}
database->generateDB();
MPI_File_close(&inMPI);
MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
#else
//need to know number of template seqs for suffixdb
if (method == "suffix") {
ifstream inFASTA;
m->openInputFile(tempFile, inFASTA);
m->getNumSeqs(inFASTA, numSeqs);
inFASTA.close();
}
bool needToGenerate = true;
string kmerDBName;
if(method == "kmer") {
database = new KmerDB(tempFile, kmerSize);
kmerDBName = tempFile.substr(0,tempFile.find_last_of(".")+1) + char('0'+ kmerSize) + "mer";
ifstream kmerFileTest(kmerDBName.c_str());
if(kmerFileTest){
bool GoodFile = m->checkReleaseVersion(kmerFileTest, m->getVersion());
if (GoodFile) { needToGenerate = false; }
}
}
else if(method == "suffix") { database = new SuffixDB(numSeqs); }
else if(method == "blast") { database = new BlastDB(tempFile.substr(0,tempFile.find_last_of(".")+1), gapOpen, gapExtend, match, misMatch, "", threadID); }
else if(method == "distance") { database = new DistanceDB(); }
else {
m->mothurOut(method + " is not a valid search option. I will run the command using kmer, ksize=8.");
m->mothurOutEndLine();
database = new KmerDB(tempFile, 8);
}
if (needToGenerate) {
ifstream fastaFile;
m->openInputFile(tempFile, fastaFile);
while (!fastaFile.eof()) {
Sequence temp(fastaFile);
m->gobble(fastaFile);
if (rdb->save) { rdb->referenceSeqs.push_back(temp); }
names.push_back(temp.getName());
database->addSequence(temp);
}
fastaFile.close();
if ((method == "kmer") && (!shortcuts)) {;} //don't print
else {database->generateDB(); }
}else if ((method == "kmer") && (!needToGenerate)) {
ifstream kmerFileTest(kmerDBName.c_str());
database->readKmerDB(kmerFileTest);
ifstream fastaFile;
m->openInputFile(tempFile, fastaFile);
while (!fastaFile.eof()) {
Sequence temp(fastaFile);
m->gobble(fastaFile);
if (rdb->save) { rdb->referenceSeqs.push_back(temp); }
names.push_back(temp.getName());
}
fastaFile.close();
}
#endif
database->setNumSeqs(names.size());
m->mothurOut("DONE."); m->mothurOutEndLine();
m->mothurOut("It took " + toString(time(NULL) - start) + " seconds generate search database. "); m->mothurOutEndLine();
}
readTaxonomy(taxFile);
//sanity check
bool okay = phyloTree->ErrorCheck(names);
if (!okay) { m->control_pressed = true; }
}
catch(exception& e) {
m->errorOut(e, "Classify", "generateDatabaseAndNames");
exit(1);
}
}
/**************************************************************************************************/
Classify::Classify() { m = MothurOut::getInstance(); database = NULL; phyloTree=NULL; flipped=false; }
/**************************************************************************************************/
int Classify::readTaxonomy(string file) {
try {
phyloTree = new PhyloTree();
string name, taxInfo;
m->mothurOutEndLine();
m->mothurOut("Reading in the " + file + " taxonomy...\t"); cout.flush();
if (m->debug) { m->mothurOut("[DEBUG]: Taxonomies read in...\n"); }
#ifdef USE_MPI
int pid, num, processors;
vector<unsigned long long> positions;
int tag = 2001;
MPI_Status status;
MPI_File inMPI;
MPI_Comm_rank(MPI_COMM_WORLD, &pid); //find out who we are
MPI_Comm_size(MPI_COMM_WORLD, &processors);
char inFileName[1024];
strcpy(inFileName, file.c_str());
MPI_File_open(MPI_COMM_WORLD, inFileName, MPI_MODE_RDONLY, MPI_INFO_NULL, &inMPI); //comm, filename, mode, info, filepointer
//delete inFileName;
if (pid == 0) {
positions = m->setFilePosEachLine(file, num);
//send file positions to all processes
for(int i = 1; i < processors; i++) {
MPI_Send(&num, 1, MPI_INT, i, tag, MPI_COMM_WORLD);
MPI_Send(&positions[0], (num+1), MPI_LONG, i, tag, MPI_COMM_WORLD);
}
}else{
MPI_Recv(&num, 1, MPI_INT, 0, tag, MPI_COMM_WORLD, &status);
positions.resize(num+1);
MPI_Recv(&positions[0], (num+1), MPI_LONG, 0, tag, MPI_COMM_WORLD, &status);
}
//read file
for(int i=0;i<num;i++){
//read next sequence
int length = positions[i+1] - positions[i];
char* buf4 = new char[length];
MPI_File_read_at(inMPI, positions[i], buf4, length, MPI_CHAR, &status);
string tempBuf = buf4;
if (tempBuf.length() > length) { tempBuf = tempBuf.substr(0, length); }
delete buf4;
istringstream iss (tempBuf,istringstream::in);
iss >> name; m->gobble(iss);
iss >> taxInfo;
if (m->debug) { m->mothurOut("[DEBUG]: name = " + name + " tax = " + taxInfo + "\n"); }
//commented out to save time with large templates. 10/7/13
//if (m->inUsersGroups(name, names)) {
taxonomy[name] = taxInfo;
phyloTree->addSeqToTree(name, taxInfo);
//}else {
// m->mothurOut("[WARNING]: " + name + " is in your taxonomy file and not in your reference file, ignoring.\n");
//}
}
MPI_File_close(&inMPI);
MPI_Barrier(MPI_COMM_WORLD); //make everyone wait - just in case
#else
taxonomy.clear();
m->readTax(file, taxonomy);
//commented out to save time with large templates. 6/12/13
//map<string, string> tempTaxonomy;
for (map<string, string>::iterator itTax = taxonomy.begin(); itTax != taxonomy.end(); itTax++) {
//if (m->inUsersGroups(itTax->first, names)) {
phyloTree->addSeqToTree(itTax->first, itTax->second);
if (m->control_pressed) { break; }
//tempTaxonomy[itTax->first] = itTax->second;
// }else {
// m->mothurOut("[WARNING]: " + itTax->first + " is in your taxonomy file and not in your reference file, ignoring.\n");
//}
}
//taxonomy = tempTaxonomy;
#endif
phyloTree->assignHeirarchyIDs(0);
phyloTree->setUp(file);
m->mothurOut("DONE.");
m->mothurOutEndLine(); cout.flush();
return phyloTree->getNumSeqs();
}
catch(exception& e) {
m->errorOut(e, "Classify", "readTaxonomy");
exit(1);
}
}
/**************************************************************************************************/
vector<string> Classify::parseTax(string tax) {
try {
vector<string> taxons;
m->splitAtChar(tax, taxons, ';');
return taxons;
}
catch(exception& e) {
m->errorOut(e, "Classify", "parseTax");
exit(1);
}
}
/**************************************************************************************************/
double Classify::getLogExpSum(vector<double> probabilities, int& maxIndex){
try {
// http://jblevins.org/notes/log-sum-exp
double maxProb = probabilities[0];
maxIndex = 0;
int numProbs = (int)probabilities.size();
for(int i=1;i<numProbs;i++){
if(probabilities[i] >= maxProb){
maxProb = probabilities[i];
maxIndex = i;
}
}
double probSum = 0.0000;
for(int i=0;i<numProbs;i++){
probSum += exp(probabilities[i] - maxProb);
}
probSum = log(probSum) + maxProb;
return probSum;
}
catch(exception& e) {
m->errorOut(e, "Classify", "getLogExpSum");
exit(1);
}
}
/**************************************************************************************************/
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