File: heatmapcommand.cpp

package info (click to toggle)
mothur 1.33.3%2Bdfsg-2
  • links: PTS, VCS
  • area: main
  • in suites: jessie, jessie-kfreebsd
  • size: 11,248 kB
  • ctags: 12,231
  • sloc: cpp: 152,046; fortran: 665; makefile: 74; sh: 34
file content (556 lines) | stat: -rw-r--r-- 24,306 bytes parent folder | download
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
/*
 *  heatmapcommand.cpp
 *  Mothur
 *
 *  Created by Sarah Westcott on 3/25/09.
 *  Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
 *
 */

#include "heatmapcommand.h"

//**********************************************************************************************************************
vector<string> HeatMapCommand::setParameters(){	
	try {
		CommandParameter plist("list", "InputTypes", "", "", "LRSS", "LRSS", "none","svg",false,false,true); parameters.push_back(plist);
		CommandParameter prabund("rabund", "InputTypes", "", "", "LRSS", "LRSS", "none","svg",false,false); parameters.push_back(prabund);
		CommandParameter psabund("sabund", "InputTypes", "", "", "LRSS", "LRSS", "none","svg",false,false); parameters.push_back(psabund);
		CommandParameter pshared("shared", "InputTypes", "", "", "LRSS", "LRSS", "none","svg",false,false,true); parameters.push_back(pshared);	
		CommandParameter prelabund("relabund", "InputTypes", "", "", "LRSS", "LRSS", "none","svg",false,false); parameters.push_back(prelabund);
		CommandParameter pgroups("groups", "String", "", "", "", "", "","",false,false); parameters.push_back(pgroups);
		CommandParameter pscale("scale", "Multiple", "log10-log2-linear", "log10", "", "", "","",false,false); parameters.push_back(pscale);
		CommandParameter psorted("sorted", "Multiple", "none-shared-topotu-topgroup", "shared", "", "", "","",false,false); parameters.push_back(psorted);
		CommandParameter pnumotu("numotu", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pnumotu);
		CommandParameter pfontsize("fontsize", "Number", "", "24", "", "", "","",false,false); parameters.push_back(pfontsize);
		CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
		CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
		CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
		
		vector<string> myArray;
		for (int i = 0; i < parameters.size(); i++) {	myArray.push_back(parameters[i].name);		}
		return myArray;
	}
	catch(exception& e) {
		m->errorOut(e, "HeatMapCommand", "setParameters");
		exit(1);
	}
}
//**********************************************************************************************************************
string HeatMapCommand::getHelpString(){	
	try {
		string helpString = "";
		helpString += "The heatmap.bin command parameters are shared, relabund, list, rabund, sabund, groups, sorted, scale, numotu, fontsize and label.  shared, relabund, list, rabund or sabund is required unless you have a valid current file.\n";
		helpString += "The groups parameter allows you to specify which of the groups in your groupfile you would like included in your heatmap.\n";
		helpString += "The sorted parameter allows you to order the otus displayed, default=shared, meaning display the shared otus first. Other options for sorted are none, meaning the exact representation of your otus, \n";
		helpString += "topotu, meaning the otus with the greatest abundance when totaled across groups, topgroup, meaning the top otus for each group. \n";
		helpString += "The scale parameter allows you to choose the range of color your bin information will be displayed with.\n";
		helpString += "The numotu parameter allows you to display only the top N otus, by default all the otus are displayed. You could choose to look at the top 10, by setting numotu=10. The default for sorted is topotu when numotu is used.\n";
		helpString += "The group names are separated by dashes. The label parameter allows you to select what distance levels you would like a heatmap created for, and are also separated by dashes.\n";
		helpString += "The fontsize parameter allows you to adjust the font size of the picture created, default=24.\n";
		helpString += "The heatmap.bin command should be in the following format: heatmap.bin(groups=yourGroups, sorted=yourSorted, label=yourLabels).\n";
		helpString += "Example heatmap.bin(groups=A-B-C, sorted=none, scale=log10).\n";
		helpString += "The default value for groups is all the groups in your groupfile, and all labels in your inputfile will be used.\n";
		helpString += "The default value for scale is log10; your other options are log2 and linear.\n";
		helpString += "The heatmap.bin command outputs a .svg file for each label you specify.\n";
		helpString += "Note: No spaces between parameter labels (i.e. groups), '=' and parameters (i.e.yourGroups).\n";
		return helpString;
	}
	catch(exception& e) {
		m->errorOut(e, "HeatMapCommand", "getHelpString");
		exit(1);
	}
}

//**********************************************************************************************************************
string HeatMapCommand::getOutputPattern(string type) {
    try {
        string pattern = "";
        
        if (type == "svg") {  pattern = "[filename],svg"; } 
        else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true;  }
        
        return pattern;
    }
    catch(exception& e) {
        m->errorOut(e, "HeatMapCommand", "getOutputPattern");
        exit(1);
    }
}

//**********************************************************************************************************************
HeatMapCommand::HeatMapCommand(){	
	try {
		abort = true; calledHelp = true; 
		setParameters();
		vector<string> tempOutNames;
		outputTypes["svg"] = tempOutNames;
	}
	catch(exception& e) {
		m->errorOut(e, "HeatMapCommand", "HeatMapCommand");
		exit(1);
	}
}
//**********************************************************************************************************************

HeatMapCommand::HeatMapCommand(string option) {
	try {
		abort = false; calledHelp = false;   
		allLines = 1;
		
		//allow user to run help
		if(option == "help") { help(); abort = true; calledHelp = true; }
		else if(option == "citation") { citation(); abort = true; calledHelp = true;}
		
		else {
			vector<string> myArray = setParameters();
			
			OptionParser parser(option);
			map<string,string> parameters = parser.getParameters();
			map<string,string>::iterator it;
			
			ValidParameters validParameter;
			
			//check to make sure all parameters are valid for command
			for (it = parameters.begin(); it != parameters.end(); it++) { 
				if (validParameter.isValidParameter(it->first, myArray, it->second) != true) {  abort = true;  }
			}
			
			//initialize outputTypes
			vector<string> tempOutNames;
			outputTypes["svg"] = tempOutNames;
		
			//if the user changes the input directory command factory will send this info to us in the output parameter 
			string inputDir = validParameter.validFile(parameters, "inputdir", false);		
			if (inputDir == "not found"){	inputDir = "";		}
			else {
				string path;
				it = parameters.find("shared");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["shared"] = inputDir + it->second;		}
				}
				
				it = parameters.find("rabund");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["rabund"] = inputDir + it->second;		}
				}
				
				it = parameters.find("sabund");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["sabund"] = inputDir + it->second;		}
				}
				
				it = parameters.find("list");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["list"] = inputDir + it->second;		}
				}
				
				it = parameters.find("relabund");
				//user has given a template file
				if(it != parameters.end()){ 
					path = m->hasPath(it->second);
					//if the user has not given a path then, add inputdir. else leave path alone.
					if (path == "") {	parameters["relabund"] = inputDir + it->second;		}
				}
			}
			
			//check for required parameters
			listfile = validParameter.validFile(parameters, "list", true);
			if (listfile == "not open") { abort = true; }
			else if (listfile == "not found") { listfile = ""; }
			else {  format = "list"; inputfile = listfile; m->setListFile(listfile); }
			
			sabundfile = validParameter.validFile(parameters, "sabund", true);
			if (sabundfile == "not open") {  abort = true; }	
			else if (sabundfile == "not found") { sabundfile = ""; }
			else {  format = "sabund"; inputfile = sabundfile; m->setSabundFile(sabundfile); }
			
			rabundfile = validParameter.validFile(parameters, "rabund", true);
			if (rabundfile == "not open") {  abort = true; }	
			else if (rabundfile == "not found") { rabundfile = ""; }
			else {  format = "rabund"; inputfile = rabundfile; m->setRabundFile(rabundfile); }
			
			sharedfile = validParameter.validFile(parameters, "shared", true);
			if (sharedfile == "not open") { abort = true; }	
			else if (sharedfile == "not found") { sharedfile = ""; }
			else {  format = "sharedfile"; inputfile = sharedfile; m->setSharedFile(sharedfile); }
			
			relabundfile = validParameter.validFile(parameters, "relabund", true);
			if (relabundfile == "not open") {  abort = true; }	
			else if (relabundfile == "not found") { relabundfile = ""; }
			else {  format = "relabund"; inputfile = relabundfile; m->setRelAbundFile(relabundfile); }
			
			
			if ((sharedfile == "") && (listfile == "") && (rabundfile == "") && (sabundfile == "") && (relabundfile == "")) { 
				//is there are current file available for any of these?
				//give priority to shared, then list, then rabund, then sabund
				//if there is a current shared file, use it
				sharedfile = m->getSharedFile(); 
				if (sharedfile != "") { inputfile = sharedfile; format = "sharedfile"; m->mothurOut("Using " + sharedfile + " as input file for the shared parameter."); m->mothurOutEndLine(); }
				else { 
					listfile = m->getListFile(); 
					if (listfile != "") { inputfile = listfile; format = "list"; m->mothurOut("Using " + listfile + " as input file for the list parameter."); m->mothurOutEndLine(); }
					else { 
						rabundfile = m->getRabundFile(); 
						if (rabundfile != "") { inputfile = rabundfile; format = "rabund"; m->mothurOut("Using " + rabundfile + " as input file for the rabund parameter."); m->mothurOutEndLine(); }
						else { 
							sabundfile = m->getSabundFile(); 
							if (sabundfile != "") { inputfile = sabundfile; format = "sabund"; m->mothurOut("Using " + sabundfile + " as input file for the sabund parameter."); m->mothurOutEndLine(); }
							else { 
								relabundfile = m->getRelAbundFile(); 
								if (relabundfile != "") { inputfile = relabundfile; format = "relabund"; m->mothurOut("Using " + relabundfile + " as input file for the relabund parameter."); m->mothurOutEndLine(); }
								else { 
									m->mothurOut("No valid current files. You must provide a list, sabund, rabund, relabund or shared file."); m->mothurOutEndLine(); 
									abort = true;
								}
							}
						}
					}
				}
			}
			
			
			//if the user changes the output directory command factory will send this info to us in the output parameter 
			outputDir = validParameter.validFile(parameters, "outputdir", false);		if (outputDir == "not found"){	outputDir = m->hasPath(inputfile);		}
			
			//check for optional parameter and set defaults
			// ...at some point should added some additional type checking...
			label = validParameter.validFile(parameters, "label", false);			
			if (label == "not found") { label = ""; }
			else { 
				if(label != "all") {  m->splitAtDash(label, labels);  allLines = 0;  }
				else { allLines = 1;  }
			}
			
			
			groups = validParameter.validFile(parameters, "groups", false);			
			if (groups == "not found") { groups = ""; }
			else { 
				m->splitAtDash(groups, Groups);
				m->setGroups(Groups);
			}
			
			string temp = validParameter.validFile(parameters, "numotu", false);		if (temp == "not found") { temp = "0"; }
			m->mothurConvert(temp, numOTU);
			
			temp = validParameter.validFile(parameters, "fontsize", false);				if (temp == "not found") { temp = "24"; }
			m->mothurConvert(temp, fontSize);
			
			sorted = validParameter.validFile(parameters, "sorted", false);				
			if (sorted == "not found") { 
				//if numOTU is used change default
				if (numOTU != 0) { sorted = "topotu"; }
				else { sorted = "shared"; }
			}
		 
			scale = validParameter.validFile(parameters, "scale", false);				if (scale == "not found") { scale = "log10"; }
			
			if ((sorted != "none") && (sorted != "shared") && (sorted != "topotu") && (sorted != "topgroup")) { m->mothurOut(sorted + " is not a valid sorting option. Sorted options are: none, shared, topotu, topgroup"); m->mothurOutEndLine(); abort=true;  }
		}

	}
	catch(exception& e) {
		m->errorOut(e, "HeatMapCommand", "HeatMapCommand");
		exit(1);
	}
}
//**********************************************************************************************************************

int HeatMapCommand::execute(){
	try {
	
		if (abort == true) { if (calledHelp) { return 0; }  return 2;	}
		
		heatmap = new HeatMap(sorted, scale, numOTU, fontSize, outputDir, inputfile);

		string lastLabel;
		input = new InputData(inputfile, format);
		
		if (format == "sharedfile") {
			//you have groups
			lookup = input->getSharedRAbundVectors();
			lastLabel = lookup[0]->getLabel();
	
		}else if ((format == "list") || (format == "rabund") || (format == "sabund")) {
			//you are using just a list file and have only one group
			rabund = input->getRAbundVector();
			lastLabel = rabund->getLabel();
		}else if (format == "relabund") {
			//you have groups
			lookupFloat = input->getSharedRAbundFloatVectors();
			lastLabel = lookupFloat[0]->getLabel();
		}
		
		//if the users enters label "0.06" and there is no "0.06" in their file use the next lowest label.
		set<string> processedLabels;
		set<string> userLabels = labels;

		if (format == "sharedfile") {	
		
			//as long as you are not at the end of the file or done wih the lines you want
			while((lookup[0] != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
				if (m->control_pressed) {
					for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  }
					for (int i = 0; i < outputNames.size(); i++) {	if (outputNames[i] != "control") {  m->mothurRemove(outputNames[i]);  } } outputTypes.clear();
					m->clearGroups(); 
					delete input; delete heatmap; return 0;
				}
		
				if(allLines == 1 || labels.count(lookup[0]->getLabel()) == 1){			
	
					m->mothurOut(lookup[0]->getLabel()); m->mothurOutEndLine();
					string outputFileName = heatmap->getPic(lookup);
					outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
					
					processedLabels.insert(lookup[0]->getLabel());
					userLabels.erase(lookup[0]->getLabel());
				}
				
				if ((m->anyLabelsToProcess(lookup[0]->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
					string saveLabel = lookup[0]->getLabel();
			
					for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  }  
			
					lookup = input->getSharedRAbundVectors(lastLabel);
					m->mothurOut(lookup[0]->getLabel()); m->mothurOutEndLine();
					
					string outputFileName = heatmap->getPic(lookup);
					outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
					
					processedLabels.insert(lookup[0]->getLabel());
					userLabels.erase(lookup[0]->getLabel());
					
					//restore real lastlabel to save below
					lookup[0]->setLabel(saveLabel);
				}
				
				lastLabel = lookup[0]->getLabel();
				//prevent memory leak
				for (int i = 0; i < lookup.size(); i++) {  delete lookup[i]; lookup[i] = NULL; }
							
				//get next line to process
				lookup = input->getSharedRAbundVectors();				
			}
			
			
			if (m->control_pressed) {
				for (int i = 0; i < outputNames.size(); i++) {	if (outputNames[i] != "control") {  m->mothurRemove(outputNames[i]);  } } outputTypes.clear();
				m->clearGroups(); 
				delete input; delete heatmap; return 0;
			}

			//output error messages about any remaining user labels
			set<string>::iterator it;
			bool needToRun = false;
			for (it = userLabels.begin(); it != userLabels.end(); it++) {  
				m->mothurOut("Your file does not include the label " + *it); 
				if (processedLabels.count(lastLabel) != 1) {
					m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
					needToRun = true;
				}else {
					m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
				}
			}
		
			//run last label if you need to
			if (needToRun == true)  {
				for (int i = 0; i < lookup.size(); i++) { if (lookup[i] != NULL) { delete lookup[i]; } }  
				lookup = input->getSharedRAbundVectors(lastLabel);
				
				m->mothurOut(lookup[0]->getLabel()); m->mothurOutEndLine();
				string outputFileName = heatmap->getPic(lookup);
				outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
				for (int i = 0; i < lookup.size(); i++) {  delete lookup[i];  }
			}
		
			//reset groups parameter
			m->clearGroups();  
			
		}else if ((format == "list") || (format == "rabund") || (format == "sabund")) {
	
			while((rabund != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
				if (m->control_pressed) {   
					for (int i = 0; i < outputNames.size(); i++) {	if (outputNames[i] != "control") {  m->mothurRemove(outputNames[i]);  } } outputTypes.clear();
					delete rabund;  delete input; delete heatmap; return 0;	
				}

				if(allLines == 1 || labels.count(rabund->getLabel()) == 1){			
	
					m->mothurOut(rabund->getLabel()); m->mothurOutEndLine();
					string outputFileName = heatmap->getPic(rabund);
					outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
					
					processedLabels.insert(rabund->getLabel());
					userLabels.erase(rabund->getLabel());
				}
				
				if ((m->anyLabelsToProcess(rabund->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
					string saveLabel = rabund->getLabel();
					
					delete rabund;
					rabund = input->getRAbundVector(lastLabel);
					m->mothurOut(rabund->getLabel()); m->mothurOutEndLine();
					
					string outputFileName = heatmap->getPic(rabund);
					outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
					
					processedLabels.insert(rabund->getLabel());
					userLabels.erase(rabund->getLabel());
					
					//restore real lastlabel to save below
					rabund->setLabel(saveLabel);
				}		
				
								
								
				lastLabel = rabund->getLabel();			
				delete rabund;
				rabund = input->getRAbundVector();
			}
			
			if (m->control_pressed) {
				for (int i = 0; i < outputNames.size(); i++) {	if (outputNames[i] != "control") {  m->mothurRemove(outputNames[i]);  } } outputTypes.clear();
				delete input; delete heatmap; return 0;
			}

			//output error messages about any remaining user labels
			set<string>::iterator it;
			bool needToRun = false;
			for (it = userLabels.begin(); it != userLabels.end(); it++) {  
				m->mothurOut("Your file does not include the label " + *it); 
				if (processedLabels.count(lastLabel) != 1) {
					m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
					needToRun = true;
				}else {
					m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
				}
			}
		
			//run last label if you need to
			if (needToRun == true)  {
		
				if (rabund != NULL) {	delete rabund;	}
				rabund = input->getRAbundVector(lastLabel);
				m->mothurOut(rabund->getLabel()); m->mothurOutEndLine();
					
				string outputFileName = heatmap->getPic(rabund);
				outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
				delete rabund; 
			}
		
		}else {
		
			//as long as you are not at the end of the file or done wih the lines you want
			while((lookupFloat[0] != NULL) && ((allLines == 1) || (userLabels.size() != 0))) {
				if (m->control_pressed) {
					for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  }
					for (int i = 0; i < outputNames.size(); i++) {	if (outputNames[i] != "control") {  m->mothurRemove(outputNames[i]);  } } outputTypes.clear();
					m->clearGroups(); 
					delete input; delete heatmap; return 0;
				}
		
				if(allLines == 1 || labels.count(lookupFloat[0]->getLabel()) == 1){			
	
					m->mothurOut(lookupFloat[0]->getLabel()); m->mothurOutEndLine();
					string outputFileName = heatmap->getPic(lookupFloat);
					outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
					
					processedLabels.insert(lookupFloat[0]->getLabel());
					userLabels.erase(lookupFloat[0]->getLabel());
				}
				
				if ((m->anyLabelsToProcess(lookupFloat[0]->getLabel(), userLabels, "") == true) && (processedLabels.count(lastLabel) != 1)) {
					string saveLabel = lookupFloat[0]->getLabel();
				
					for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  }  
					lookupFloat = input->getSharedRAbundFloatVectors(lastLabel);
					m->mothurOut(lookupFloat[0]->getLabel()); m->mothurOutEndLine();
					
					string outputFileName = heatmap->getPic(lookupFloat);
					outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
					
					processedLabels.insert(lookupFloat[0]->getLabel());
					userLabels.erase(lookupFloat[0]->getLabel());
					
					//restore real lastlabel to save below
					lookupFloat[0]->setLabel(saveLabel);
				}
				
				lastLabel = lookupFloat[0]->getLabel();
				//prevent memory leak
				for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i]; lookupFloat[i] = NULL; }
							
				//get next line to process
				lookupFloat = input->getSharedRAbundFloatVectors();				
			}
			
			
			if (m->control_pressed) {
				for (int i = 0; i < outputNames.size(); i++) {	if (outputNames[i] != "control") {  m->mothurRemove(outputNames[i]);  } } outputTypes.clear();
				m->clearGroups(); 
				delete input; delete heatmap; return 0;
			}

			//output error messages about any remaining user labels
			set<string>::iterator it;
			bool needToRun = false;
			for (it = userLabels.begin(); it != userLabels.end(); it++) {  
				m->mothurOut("Your file does not include the label " + *it); 
				if (processedLabels.count(lastLabel) != 1) {
					m->mothurOut(". I will use " + lastLabel + "."); m->mothurOutEndLine();
					needToRun = true;
				}else {
					m->mothurOut(". Please refer to " + lastLabel + "."); m->mothurOutEndLine();
				}
			}
		
			//run last label if you need to
			if (needToRun == true)  {
				for (int i = 0; i < lookupFloat.size(); i++) { if (lookupFloat[i] != NULL) { delete lookupFloat[i]; } }  
				lookupFloat = input->getSharedRAbundFloatVectors(lastLabel);
				
				m->mothurOut(lookupFloat[0]->getLabel()); m->mothurOutEndLine();
				string outputFileName = heatmap->getPic(lookupFloat);
				outputNames.push_back(outputFileName); outputTypes["svg"].push_back(outputFileName);
				for (int i = 0; i < lookupFloat.size(); i++) {  delete lookupFloat[i];  }
			}
		
			//reset groups parameter
			m->clearGroups();  

		}
		
		delete input; 
		delete heatmap;
		
		if (m->control_pressed) {
			for (int i = 0; i < outputNames.size(); i++) {	if (outputNames[i] != "control") {  m->mothurRemove(outputNames[i]);  } } outputTypes.clear(); return 0;
		}
		
		m->mothurOutEndLine();
		m->mothurOut("Output File Names: "); m->mothurOutEndLine();
		for (int i = 0; i < outputNames.size(); i++) {	m->mothurOut(outputNames[i]); m->mothurOutEndLine();	}
		m->mothurOutEndLine();

		return 0;
	}
	catch(exception& e) {
		m->errorOut(e, "HeatMapCommand", "execute");
		exit(1);
	}
}

//**********************************************************************************************************************