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/*
* libshuffcommand.cpp
* Mothur
*
* Created by Sarah Westcott on 3/9/09.
* Copyright 2009 Schloss Lab UMASS Amherst. All rights reserved.
*
*/
/* This class is designed to implement an integral form of the Cramer-von Mises statistic.
you may refer to the "Integration of Microbial Ecology and Statistics: A Test To Compare Gene Libraries"
paper in Applied and Environmental Microbiology, Sept. 2004, p. 5485-5492 0099-2240/04/$8.00+0
DOI: 10.1128/AEM.70.9.5485-5492.2004 Copyright 2004 American Society for Microbiology for more information. */
#include "libshuffcommand.h"
#include "libshuff.h"
#include "slibshuff.h"
#include "dlibshuff.h"
//**********************************************************************************************************************
vector<string> LibShuffCommand::setParameters(){
try {
CommandParameter pphylip("phylip", "InputTypes", "", "", "none", "none", "none","coverage-libshuffsummary",false,true,true); parameters.push_back(pphylip);
CommandParameter pgroup("group", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(pgroup);
CommandParameter pgroups("groups", "String", "", "", "", "", "","",false,false); parameters.push_back(pgroups);
CommandParameter piters("iters", "Number", "", "10000", "", "", "","",false,false); parameters.push_back(piters);
CommandParameter pstep("step", "Number", "", "0.01", "", "", "","",false,false); parameters.push_back(pstep);
CommandParameter pcutoff("cutoff", "Number", "", "1.0", "", "", "","",false,false); parameters.push_back(pcutoff);
CommandParameter pform("form", "Multiple", "discrete-integral", "integral", "", "", "","",false,false); parameters.push_back(pform);
CommandParameter psim("sim", "Boolean", "", "F", "", "", "","",false,false); parameters.push_back(psim);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string LibShuffCommand::getHelpString(){
try {
string helpString = "";
helpString += "The libshuff command parameters are phylip, group, sim, groups, iters, step, form and cutoff. phylip and group parameters are required, unless you have valid current files.\n";
helpString += "The groups parameter allows you to specify which of the groups in your groupfile you would like analyzed. You must enter at least 2 valid groups.\n";
helpString += "The group names are separated by dashes. The iters parameter allows you to specify how many random matrices you would like compared to your matrix.\n";
helpString += "The step parameter allows you to specify change in distance you would like between each output if you are using the discrete form.\n";
helpString += "The form parameter allows you to specify if you would like to analyze your matrix using the discrete or integral form. Your options are integral or discrete.\n";
helpString += "The libshuff command should be in the following format: libshuff(groups=yourGroups, iters=yourIters, cutOff=yourCutOff, form=yourForm, step=yourStep).\n";
helpString += "Example libshuff(groups=A-B-C, iters=500, form=discrete, step=0.01, cutOff=2.0).\n";
helpString += "The default value for groups is all the groups in your groupfile, iters is 10000, cutoff is 1.0, form is integral and step is 0.01.\n";
helpString += "The libshuff command output two files: .coverage and .slsummary their descriptions are in the manual.\n";
helpString += "Note: No spaces between parameter labels (i.e. iters), '=' and parameters (i.e.yourIters).\n";
return helpString;
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string LibShuffCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "coverage") { pattern = "[filename],libshuff.coverage"; }
else if (type == "libshuffsummary") { pattern = "[filename],libshuff.summary"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true; }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "getOutputPattern");
exit(1);
}
}
//**********************************************************************************************************************
LibShuffCommand::LibShuffCommand(){
try {
abort = true; calledHelp = true;
setParameters();
vector<string> tempOutNames;
outputTypes["coverage"] = tempOutNames;
outputTypes["libshuffsummary"] = tempOutNames;
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "LibShuffCommand");
exit(1);
}
}
//**********************************************************************************************************************
LibShuffCommand::LibShuffCommand(string option) {
try {
abort = false; calledHelp = false;
//allow user to run help
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
vector<string> myArray = setParameters();
OptionParser parser(option);
map<string, string> parameters = parser.getParameters();
map<string,string>::iterator it;
ValidParameters validParameter;
//check to make sure all parameters are valid for command
for (it = parameters.begin(); it != parameters.end(); it++) {
if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
}
//initialize outputTypes
vector<string> tempOutNames;
outputTypes["coverage"] = tempOutNames;
outputTypes["libshuffsummary"] = tempOutNames;
string inputDir = validParameter.validFile(parameters, "inputdir", false);
if (inputDir == "not found"){ inputDir = ""; }
else {
string path;
it = parameters.find("phylip");
//user has given a template file
if(it != parameters.end()){
path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["phylip"] = inputDir + it->second; }
}
it = parameters.find("group");
//user has given a template file
if(it != parameters.end()){
path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["group"] = inputDir + it->second; }
}
}
//check for required parameters
phylipfile = validParameter.validFile(parameters, "phylip", true);
if (phylipfile == "not open") { phylipfile = ""; abort = true; }
else if (phylipfile == "not found") {
phylipfile = m->getPhylipFile();
if (phylipfile != "") { m->mothurOut("Using " + phylipfile + " as input file for the phylip parameter."); m->mothurOutEndLine(); }
else {
m->mothurOut("You must provide a phylip file."); m->mothurOutEndLine();
abort = true;
}
}else { m->setPhylipFile(phylipfile); }
//check for required parameters
groupfile = validParameter.validFile(parameters, "group", true);
if (groupfile == "not open") { groupfile = ""; abort = true; }
else if (groupfile == "not found") {
groupfile = m->getGroupFile();
if (groupfile != "") { m->mothurOut("Using " + groupfile + " as input file for the group parameter."); m->mothurOutEndLine(); }
else {
m->mothurOut("You must provide a group file."); m->mothurOutEndLine();
abort = true;
}
}else { m->setGroupFile(groupfile); }
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){
outputDir = "";
outputDir += m->hasPath(phylipfile); //if user entered a file with a path then preserve it
}
//check for optional parameter and set defaults
// ...at some point should added some additional type checking...
groups = validParameter.validFile(parameters, "groups", false);
if (groups == "not found") { groups = ""; savegroups = groups; }
else {
savegroups = groups;
m->splitAtDash(groups, Groups);
m->setGroups(Groups);
}
string temp;
temp = validParameter.validFile(parameters, "iters", false); if (temp == "not found") { temp = "10000"; }
m->mothurConvert(temp, iters);
temp = validParameter.validFile(parameters, "cutoff", false); if (temp == "not found") { temp = "1.0"; }
m->mothurConvert(temp, cutOff);
temp = validParameter.validFile(parameters, "step", false); if (temp == "not found") { temp = "0.01"; }
m->mothurConvert(temp, step);
temp = validParameter.validFile(parameters, "sim", false); if (temp == "not found") { temp = "F"; }
sim = m->isTrue(temp);
userform = validParameter.validFile(parameters, "form", false); if (userform == "not found") { userform = "integral"; }
}
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "LibShuffCommand");
exit(1);
}
}
//**********************************************************************************************************************
int LibShuffCommand::execute(){
try {
if (abort == true) { if (calledHelp) { return 0; } return 2; }
//read files
groupMap = new GroupMap(groupfile);
int error = groupMap->readMap();
if (error == 1) { delete groupMap; return 0; }
ifstream in;
m->openInputFile(phylipfile, in);
matrix = new FullMatrix(in, groupMap, sim); //reads the matrix file
in.close();
if (m->control_pressed) { delete groupMap; delete matrix; return 0; }
//if files don't match...
if (matrix->getNumSeqs() < groupMap->getNumSeqs()) {
m->mothurOut("Your distance file contains " + toString(matrix->getNumSeqs()) + " sequences, and your group file contains " + toString(groupMap->getNumSeqs()) + " sequences."); m->mothurOutEndLine();
//create new group file
if(outputDir == "") { outputDir += m->hasPath(groupfile); }
string newGroupFile = outputDir + m->getRootName(m->getSimpleName(groupfile)) + "editted.groups";
outputNames.push_back(newGroupFile);
ofstream outGroups;
m->openOutputFile(newGroupFile, outGroups);
for (int i = 0; i < matrix->getNumSeqs(); i++) {
if (m->control_pressed) { delete groupMap; delete matrix; outGroups.close(); m->mothurRemove(newGroupFile); return 0; }
Names temp = matrix->getRowInfo(i);
outGroups << temp.seqName << '\t' << temp.groupName << endl;
}
outGroups.close();
m->mothurOut(newGroupFile + " is a new group file containing only the sequence that are in your distance file. I will read this file instead."); m->mothurOutEndLine();
//read new groupfile
delete groupMap;
groupfile = newGroupFile;
groupMap = new GroupMap(groupfile);
groupMap->readMap();
if (m->control_pressed) { delete groupMap; delete matrix; m->mothurRemove(newGroupFile); return 0; }
}
setGroups(); //set the groups to be analyzed and sorts them
if (numGroups < 2) { m->mothurOut("[ERROR]: libshuff requires at least 2 groups, you only have " + toString(numGroups) + ", aborting."); m->mothurOutEndLine(); m->control_pressed = true; }
if (m->control_pressed) { delete groupMap; delete matrix; return 0; }
/********************************************************************************************/
//this is needed because when we read the matrix we sort it into groups in alphabetical order
//the rest of the command and the classes used in this command assume specific order
/********************************************************************************************/
matrix->setGroups(groupMap->getNamesOfGroups());
vector<int> sizes;
for (int i = 0; i < (groupMap->getNamesOfGroups()).size(); i++) { sizes.push_back(groupMap->getNumSeqs((groupMap->getNamesOfGroups())[i])); }
matrix->setSizes(sizes);
if(userform == "discrete"){
form = new DLibshuff(matrix, iters, step, cutOff);
}
else{
form = new SLibshuff(matrix, iters, cutOff);
}
savedDXYValues = form->evaluateAll();
savedMinValues = form->getSavedMins();
if (m->control_pressed) { delete form; m->clearGroups(); delete matrix; delete groupMap; return 0; }
pValueCounts.resize(numGroups);
for(int i=0;i<numGroups;i++){
pValueCounts[i].assign(numGroups, 0);
}
if (m->control_pressed) { outputTypes.clear(); delete form; m->clearGroups(); delete matrix; delete groupMap; return 0; }
Progress* reading = new Progress();
for(int i=0;i<numGroups-1;i++) {
for(int j=i+1;j<numGroups;j++) {
if (m->control_pressed) { outputTypes.clear(); delete form; m->clearGroups(); delete matrix; delete groupMap; delete reading; return 0; }
reading->newLine(groupNames[i]+'-'+groupNames[j], iters);
int spoti = groupMap->groupIndex[groupNames[i]]; //neccessary in case user selects groups so you know where they are in the matrix
int spotj = groupMap->groupIndex[groupNames[j]];
for(int p=0;p<iters;p++) {
if (m->control_pressed) { outputTypes.clear(); delete form; m->clearGroups(); delete matrix; delete groupMap; delete reading; return 0; }
form->randomizeGroups(spoti,spotj);
if(form->evaluatePair(spoti,spotj) >= savedDXYValues[spoti][spotj]) { pValueCounts[i][j]++; }
if(form->evaluatePair(spotj,spoti) >= savedDXYValues[spotj][spoti]) { pValueCounts[j][i]++; }
if (m->control_pressed) { outputTypes.clear(); delete form; m->clearGroups(); delete matrix; delete groupMap; delete reading; return 0; }
reading->update(p);
}
form->resetGroup(spoti);
form->resetGroup(spotj);
}
}
if (m->control_pressed) { outputTypes.clear(); delete form; m->clearGroups(); delete matrix; delete groupMap; delete reading; return 0; }
reading->finish();
delete reading;
m->mothurOutEndLine();
printSummaryFile();
printCoverageFile();
//clear out users groups
m->clearGroups();
delete form;
delete matrix; delete groupMap;
if (m->control_pressed) { outputTypes.clear(); for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
m->mothurOutEndLine();
m->mothurOut("Output File Names: "); m->mothurOutEndLine();
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
int LibShuffCommand::printCoverageFile() {
try {
ofstream outCov;
map<string, string> variables;
variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(phylipfile));
summaryFile = getOutputFileName("coverage", variables);
m->openOutputFile(summaryFile, outCov);
outputNames.push_back(summaryFile); outputTypes["coverage"].push_back(summaryFile);
outCov.setf(ios::fixed, ios::floatfield); outCov.setf(ios::showpoint);
//cout.setf(ios::fixed, ios::floatfield); cout.setf(ios::showpoint);
map<double,vector<int> > allDistances;
map<double,vector<int> >::iterator it;
vector<vector<int> > indices(numGroups);
int numIndices = numGroups * numGroups;
int index = 0;
for(int i=0;i<numGroups;i++){
indices[i].assign(numGroups,0);
for(int j=0;j<numGroups;j++){
indices[i][j] = index++;
int spoti = groupMap->groupIndex[groupNames[i]]; //neccessary in case user selects groups so you know where they are in the matrix
int spotj = groupMap->groupIndex[groupNames[j]];
for(int k=0;k<savedMinValues[spoti][spotj].size();k++){
if(m->control_pressed) { outCov.close(); return 0; }
if(allDistances[savedMinValues[spoti][spotj][k]].size() != 0){
allDistances[savedMinValues[spoti][spotj][k]][indices[i][j]]++;
}
else{
allDistances[savedMinValues[spoti][spotj][k]].assign(numIndices, 0);
allDistances[savedMinValues[spoti][spotj][k]][indices[i][j]] = 1;
}
}
}
}
it=allDistances.begin();
//cout << setprecision(8);
vector<int> prevRow = it->second;
it++;
for(;it!=allDistances.end();it++){
for(int i=0;i<it->second.size();i++){
it->second[i] += prevRow[i];
}
prevRow = it->second;
}
vector<int> lastRow = allDistances.rbegin()->second;
outCov << setprecision(8);
outCov << "dist";
for (int i = 0; i < numGroups; i++){
outCov << '\t' << groupNames[i];
}
for (int i=0;i<numGroups;i++){
for(int j=i+1;j<numGroups;j++){
if(m->control_pressed) { outCov.close(); return 0; }
outCov << '\t' << groupNames[i] << '-' << groupNames[j] << '\t';
outCov << groupNames[j] << '-' << groupNames[i];
}
}
outCov << endl;
for(it=allDistances.begin();it!=allDistances.end();it++){
outCov << it->first << '\t';
for(int i=0;i<numGroups;i++){
outCov << it->second[indices[i][i]]/(float)lastRow[indices[i][i]] << '\t';
}
for(int i=0;i<numGroups;i++){
for(int j=i+1;j<numGroups;j++){
if(m->control_pressed) { outCov.close(); return 0; }
outCov << it->second[indices[i][j]]/(float)lastRow[indices[i][j]] << '\t';
outCov << it->second[indices[j][i]]/(float)lastRow[indices[j][i]] << '\t';
}
}
outCov << endl;
}
outCov.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "printCoverageFile");
exit(1);
}
}
//**********************************************************************************************************************
int LibShuffCommand::printSummaryFile() {
try {
ofstream outSum;
map<string, string> variables;
variables["[filename]"] = outputDir + m->getRootName(m->getSimpleName(phylipfile));
summaryFile = getOutputFileName("libshuffsummary",variables);
m->openOutputFile(summaryFile, outSum);
outputNames.push_back(summaryFile); outputTypes["libshuffsummary"].push_back(summaryFile);
outSum.setf(ios::fixed, ios::floatfield); outSum.setf(ios::showpoint);
cout.setf(ios::fixed, ios::floatfield); cout.setf(ios::showpoint);
cout << setw(20) << left << "Comparison" << '\t' << setprecision(8) << "dCXYScore" << '\t' << "Significance" << endl;
m->mothurOutJustToLog("Comparison\tdCXYScore\tSignificance"); m->mothurOutEndLine();
outSum << setw(20) << left << "Comparison" << '\t' << setprecision(8) << "dCXYScore" << '\t' << "Significance" << endl;
int precision = (int)log10(iters);
for(int i=0;i<numGroups;i++){
for(int j=i+1;j<numGroups;j++){
if(m->control_pressed) { outSum.close(); return 0; }
int spoti = groupMap->groupIndex[groupNames[i]]; //neccessary in case user selects groups so you know where they are in the matrix
int spotj = groupMap->groupIndex[groupNames[j]];
if(pValueCounts[i][j]){
cout << setw(20) << left << groupNames[i]+'-'+groupNames[j] << '\t' << setprecision(8) << savedDXYValues[spoti][spotj] << '\t' << setprecision(precision) << pValueCounts[i][j]/(float)iters << endl;
m->mothurOutJustToLog(groupNames[i]+"-"+groupNames[j] + "\t" + toString(savedDXYValues[spoti][spotj]) + "\t" + toString((pValueCounts[i][j]/(float)iters))); m->mothurOutEndLine();
outSum << setw(20) << left << groupNames[i]+'-'+groupNames[j] << '\t' << setprecision(8) << savedDXYValues[spoti][spotj] << '\t' << setprecision(precision) << pValueCounts[i][j]/(float)iters << endl;
}
else{
cout << setw(20) << left << groupNames[i]+'-'+groupNames[j] << '\t' << setprecision(8) << savedDXYValues[spoti][spotj] << '\t' << '<' <<setprecision(precision) << 1/(float)iters << endl;
m->mothurOutJustToLog(groupNames[i]+"-"+groupNames[j] + "\t" + toString(savedDXYValues[spoti][spotj]) + "\t" + toString((1/(float)iters))); m->mothurOutEndLine();
outSum << setw(20) << left << groupNames[i]+'-'+groupNames[j] << '\t' << setprecision(8) << savedDXYValues[spoti][spotj] << '\t' << '<' <<setprecision(precision) << 1/(float)iters << endl;
}
if(pValueCounts[j][i]){
cout << setw(20) << left << groupNames[j]+'-'+groupNames[i] << '\t' << setprecision(8) << savedDXYValues[spotj][spoti] << '\t' << setprecision (precision) << pValueCounts[j][i]/(float)iters << endl;
m->mothurOutJustToLog(groupNames[j]+"-"+groupNames[i] + "\t" + toString(savedDXYValues[spotj][spoti]) + "\t" + toString((pValueCounts[j][i]/(float)iters))); m->mothurOutEndLine();
outSum << setw(20) << left << groupNames[j]+'-'+groupNames[i] << '\t' << setprecision(8) << savedDXYValues[spotj][spoti] << '\t' << setprecision (precision) << pValueCounts[j][i]/(float)iters << endl;
}
else{
cout << setw(20) << left << groupNames[j]+'-'+groupNames[i] << '\t' << setprecision(8) << savedDXYValues[spotj][spoti] << '\t' << '<' <<setprecision (precision) << 1/(float)iters << endl;
m->mothurOutJustToLog(groupNames[j]+"-"+groupNames[i] + "\t" + toString(savedDXYValues[spotj][spoti]) + "\t" + toString((1/(float)iters))); m->mothurOutEndLine();
outSum << setw(20) << left << groupNames[j]+'-'+groupNames[i] << '\t' << setprecision(8) << savedDXYValues[spotj][spoti] << '\t' << '<' <<setprecision (precision) << 1/(float)iters << endl;
}
}
}
outSum.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "printSummaryFile");
exit(1);
}
}
//**********************************************************************************************************************
void LibShuffCommand::setGroups() {
try {
vector<string> myGroups = m->getGroups();
//if the user has not entered specific groups to analyze then do them all
if (m->getNumGroups() == 0) {
numGroups = groupMap->getNumGroups();
for (int i=0; i < numGroups; i++) {
myGroups.push_back((groupMap->getNamesOfGroups())[i]);
}
} else {
if (savegroups != "all") {
//check that groups are valid
for (int i = 0; i < myGroups.size(); i++) {
if (groupMap->isValidGroup(myGroups[i]) != true) {
m->mothurOut(myGroups[i] + " is not a valid group, and will be disregarded."); m->mothurOutEndLine();
// erase the invalid group from globaldata->Groups
myGroups.erase(myGroups.begin()+i);
}
}
//if the user only entered invalid groups
if ((myGroups.size() == 0) || (myGroups.size() == 1)) {
numGroups = groupMap->getNumGroups();
for (int i=0; i < numGroups; i++) {
myGroups.push_back((groupMap->getNamesOfGroups())[i]);
}
m->mothurOut("When using the groups parameter you must have at least 2 valid groups. I will run the command using all the groups in your groupfile."); m->mothurOutEndLine();
} else { numGroups = myGroups.size(); }
} else { //users wants all groups
numGroups = groupMap->getNumGroups();
myGroups.clear();
for (int i=0; i < numGroups; i++) {
myGroups.push_back((groupMap->getNamesOfGroups())[i]);
}
}
}
//sort so labels match
sort(myGroups.begin(), myGroups.end());
//sort
//sort(groupMap->namesOfGroups.begin(), groupMap->namesOfGroups.end());
for (int i = 0; i < (groupMap->getNamesOfGroups()).size(); i++) { groupMap->groupIndex[(groupMap->getNamesOfGroups())[i]] = i; }
groupNames = myGroups;
m->setGroups(myGroups);
}
catch(exception& e) {
m->errorOut(e, "LibShuffCommand", "setGroups");
exit(1);
}
}
/***********************************************************/
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