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//
// mergesfffilecommand.cpp
// Mothur
//
// Created by Sarah Westcott on 1/31/14.
// Copyright (c) 2014 Schloss Lab. All rights reserved.
//
#include "mergesfffilecommand.h"
#include "endiannessmacros.h"
//**********************************************************************************************************************
vector<string> MergeSfffilesCommand::setParameters(){
try {
CommandParameter psff("sff", "InputTypes", "", "", "sffFile", "sffFile", "none","sff",false,false); parameters.push_back(psff);
CommandParameter pfile("file", "InputTypes", "", "", "sffFile", "sffFile", "none","sff",false,false); parameters.push_back(pfile);
CommandParameter poutput("output", "String", "", "", "", "", "","",false,true,true); parameters.push_back(poutput);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string MergeSfffilesCommand::getHelpString(){
try {
string helpString = "";
helpString += "The merge.sfffiles command reads a sff file or a file containing a list of sff files and merges the individual files into a single sff file. \n";
helpString += "The merge.sfffiles command parameters are sff, file and output. sff or file is required. \n";
helpString += "The sff parameter allows you to enter the sff list of sff files separated by -'s.\n";
helpString += "The file parameter allows you to provide a file containing a list of sff files to merge. \n";
helpString += "The output parameter allows you to provide an output filename. \n";
helpString += "Example sffinfo(sff=mySffFile.sff-mySecond.sff).\n";
helpString += "Note: No spaces between parameter labels (i.e. sff), '=' and parameters (i.e.yourSffFileName).\n";
return helpString;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string MergeSfffilesCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "sff") { pattern = "[filename],"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->control_pressed = true; }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "getOutputPattern");
exit(1);
}
}
//**********************************************************************************************************************
MergeSfffilesCommand::MergeSfffilesCommand(){
try {
abort = true; calledHelp = true;
setParameters();
vector<string> tempOutNames;
outputTypes["sff"] = tempOutNames;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "MergeSfffilesCommand");
exit(1);
}
}
//**********************************************************************************************************************
MergeSfffilesCommand::MergeSfffilesCommand(string option) {
try {
abort = false; calledHelp = false;
//allow user to run help
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else {
//valid paramters for this command
vector<string> myArray = setParameters();
OptionParser parser(option);
map<string, string> parameters = parser.getParameters();
map<string,string>::iterator it;
ValidParameters validParameter;
//check to make sure all parameters are valid for command
for (map<string,string>::iterator it = parameters.begin(); it != parameters.end(); it++) {
if (validParameter.isValidParameter(it->first, myArray, it->second) != true) { abort = true; }
}
//initialize outputTypes
vector<string> tempOutNames;
outputTypes["sff"] = tempOutNames;
//if the user changes the output directory command factory will send this info to us in the output parameter
outputDir = validParameter.validFile(parameters, "outputdir", false); if (outputDir == "not found"){ outputDir = ""; }
//if the user changes the input directory command factory will send this info to us in the output parameter
string inputDir = validParameter.validFile(parameters, "inputdir", false); if (inputDir == "not found"){ inputDir = ""; }
else {
it = parameters.find("file");
//user has given a template file
if(it != parameters.end()){
string path = m->hasPath(it->second);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { parameters["file"] = inputDir + it->second; }
}
}
sffFilename = validParameter.validFile(parameters, "sff", false);
if (sffFilename == "not found") { sffFilename = ""; }
else {
m->splitAtDash(sffFilename, filenames);
//go through files and make sure they are good, if not, then disregard them
for (int i = 0; i < filenames.size(); i++) {
bool ignore = false;
if (filenames[i] == "current") {
filenames[i] = m->getSFFFile();
if (filenames[i] != "") { m->mothurOut("Using " + filenames[i] + " as input file for the sff parameter where you had given current."); m->mothurOutEndLine(); }
else {
m->mothurOut("You have no current sfffile, ignoring current."); m->mothurOutEndLine(); ignore=true;
//erase from file list
filenames.erase(filenames.begin()+i);
i--;
}
}
if (!ignore) {
if (inputDir != "") {
string path = m->hasPath(filenames[i]);
//if the user has not given a path then, add inputdir. else leave path alone.
if (path == "") { filenames[i] = inputDir + filenames[i]; }
}
ifstream in;
int ableToOpen = m->openInputFile(filenames[i], in, "noerror");
//if you can't open it, try default location
if (ableToOpen == 1) {
if (m->getDefaultPath() != "") { //default path is set
string tryPath = m->getDefaultPath() + m->getSimpleName(filenames[i]);
m->mothurOut("Unable to open " + filenames[i] + ". Trying default " + tryPath); m->mothurOutEndLine();
ifstream in2;
ableToOpen = m->openInputFile(tryPath, in2, "noerror");
in2.close();
filenames[i] = tryPath;
}
}
//if you can't open it, try default location
if (ableToOpen == 1) {
if (m->getOutputDir() != "") { //default path is set
string tryPath = m->getOutputDir() + m->getSimpleName(filenames[i]);
m->mothurOut("Unable to open " + filenames[i] + ". Trying output directory " + tryPath); m->mothurOutEndLine();
ifstream in2;
ableToOpen = m->openInputFile(tryPath, in2, "noerror");
in2.close();
filenames[i] = tryPath;
}
}
in.close();
if (ableToOpen == 1) {
m->mothurOut("Unable to open " + filenames[i] + ". It will be disregarded."); m->mothurOutEndLine();
//erase from file list
filenames.erase(filenames.begin()+i);
i--;
}else { m->setSFFFile(filenames[i]); }
}
}
}
file = validParameter.validFile(parameters, "file", true);
if (file == "not open") { abort = true; }
else if (file == "not found") { file = ""; }
if ((file == "") && (filenames.size() == 0)) {
m->mothurOut("[ERROR]: no valid files."); m->mothurOutEndLine(); abort = true;
}
if ((file != "") && (filenames.size() != 0)) { //both are given
m->mothurOut("[ERROR]: cannot use file option and sff option at the same time, choose one."); m->mothurOutEndLine(); abort = true;
}
outputFile = validParameter.validFile(parameters, "output", false);
if (outputFile == "not found") { m->mothurOut("you must enter an output file name"); m->mothurOutEndLine(); abort=true; }
if (outputDir != "") { outputFile = outputDir + m->getSimpleName(outputFile); }
}
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "MergeSfffilesCommand");
exit(1);
}
}
//**********************************************************************************************************************
int MergeSfffilesCommand::execute(){
try {
if (abort == true) { if (calledHelp) { return 0; } return 2; }
if (file != "") {
readFile();
if (outputDir == "") { outputDir = m->hasPath(file); }
}
ofstream out;
map<string, string> variables;
string thisOutputDir = outputDir;
if (outputDir == "") { thisOutputDir += m->hasPath(outputFile); }
variables["[filename]"] = thisOutputDir + m->getSimpleName(outputFile);
outputFile = getOutputFileName("sff",variables);
m->openOutputFileBinary(outputFile, out);
outputNames.push_back(outputFile); outputTypes["sff"].push_back(outputFile);
outputFileHeader = outputFile + ".headers";
numTotalReads = 0;
for (int s = 0; s < filenames.size(); s++) {
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
int start = time(NULL);
filenames[s] = m->getFullPathName(filenames[s]);
m->mothurOut("\nMerging info from " + filenames[s] + " ..." ); m->mothurOutEndLine();
int numReads = mergeSffInfo(filenames[s], out);
m->mothurOut("It took " + toString(time(NULL) - start) + " secs to merge " + toString(numReads) + ".\n");
}
out.close();
//create new common header and add to merged file
adjustCommonHeader();
if (m->control_pressed) { for (int i = 0; i < outputNames.size(); i++) { m->mothurRemove(outputNames[i]); } return 0; }
//set sff file as new current sff file
string current = "";
itTypes = outputTypes.find("sff");
if (itTypes != outputTypes.end()) {
if ((itTypes->second).size() != 0) { current = (itTypes->second)[0]; m->setSFFFile(current); }
}
//report output filenames
m->mothurOutEndLine();
m->mothurOut("Output File Names: "); m->mothurOutEndLine();
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i]); m->mothurOutEndLine(); }
m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
int MergeSfffilesCommand::mergeSffInfo(string input, ofstream& out){
try {
currentFileName = input;
ifstream in;
m->openInputFileBinary(input, in);
CommonHeader header;
readCommonHeader(in, header);
int count = 0;
//check magic number and version
if (header.magicNumber != 779314790) { m->mothurOut("Magic Number is not correct, not a valid .sff file"); m->mothurOutEndLine(); return count; }
if (header.version != "0001") { m->mothurOut("Version is not supported, only support version 0001."); m->mothurOutEndLine(); return count; }
//save for adjustHeader sanity check
commonHeaders.push_back(header);
//read through the sff file
while (!in.eof()) {
//read data
seqRead read; Header readheader;
readSeqData(in, read, header.numFlowsPerRead, readheader, out);
bool okay = sanityCheck(readheader, read);
if (!okay) { break; }
count++;
//report progress
if((count+1) % 10000 == 0){ m->mothurOut(toString(count+1)); m->mothurOutEndLine(); }
if (m->control_pressed) { count = 0; break; }
if (count >= header.numReads) { break; }
}
//report progress
if (!m->control_pressed) { if((count) % 10000 != 0){ m->mothurOut(toString(count)); m->mothurOutEndLine(); } }
in.close();
return count;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "mergeSffInfo");
exit(1);
}
}
//**********************************************************************************************************************
int MergeSfffilesCommand::readCommonHeader(ifstream& in, CommonHeader& header){
try {
if (!in.eof()) {
//read magic number
char buffer[4];
in.read(buffer, 4);
header.magicNumber = be_int4(*(unsigned int *)(&buffer));
//read version
char buffer9[4];
in.read(buffer9, 4);
header.version = "";
for (int i = 0; i < 4; i++) { header.version += toString((int)(buffer9[i])); }
//read offset
char buffer2 [8];
in.read(buffer2, 8);
header.indexOffset = be_int8(*(unsigned long long *)(&buffer2));
//read index length
char buffer3 [4];
in.read(buffer3, 4);
header.indexLength = be_int4(*(unsigned int *)(&buffer3));
//read num reads
char buffer4 [4];
in.read(buffer4, 4);
header.numReads = be_int4(*(unsigned int *)(&buffer4));
if (m->debug) { m->mothurOut("[DEBUG]: numReads = " + toString(header.numReads) + "\n"); }
//read header length
char buffer5 [2];
in.read(buffer5, 2);
header.headerLength = be_int2(*(unsigned short *)(&buffer5));
//read key length
char buffer6 [2];
in.read(buffer6, 2);
header.keyLength = be_int2(*(unsigned short *)(&buffer6));
//read number of flow reads
char buffer7 [2];
in.read(buffer7, 2);
header.numFlowsPerRead = be_int2(*(unsigned short *)(&buffer7));
//read format code
char buffer8 [1];
in.read(buffer8, 1);
header.flogramFormatCode = (int)(buffer8[0]);
//read flow chars
char* tempBuffer = new char[header.numFlowsPerRead];
in.read(&(*tempBuffer), header.numFlowsPerRead);
header.flowChars = tempBuffer;
if (header.flowChars.length() > header.numFlowsPerRead) { header.flowChars = header.flowChars.substr(0, header.numFlowsPerRead); }
delete[] tempBuffer;
//read key
char* tempBuffer2 = new char[header.keyLength];
in.read(&(*tempBuffer2), header.keyLength);
header.keySequence = tempBuffer2;
if (header.keySequence.length() > header.keyLength) { header.keySequence = header.keySequence.substr(0, header.keyLength); }
delete[] tempBuffer2;
/* Pad to 8 chars */
unsigned long long spotInFile = in.tellg();
unsigned long long spot = (spotInFile + 7)& ~7; // ~ inverts
in.seekg(spot);
}else{
m->mothurOut("Error reading sff common header."); m->mothurOutEndLine();
}
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "readCommonHeader");
exit(1);
}
}
//**********************************************************************************************************************
int MergeSfffilesCommand::adjustCommonHeader(){
try {
//sanity check
bool okay = true;
if (commonHeaders.size() != 0) {
unsigned int magicN = commonHeaders[0].magicNumber;
string version = commonHeaders[0].version;
unsigned short headerLength = commonHeaders[0].headerLength;
unsigned short keyLength = commonHeaders[0].keyLength;
unsigned short numFlows = commonHeaders[0].numFlowsPerRead;
int flowCode = commonHeaders[0].flogramFormatCode;
string flowChars = commonHeaders[0].flowChars;
string keySeq = commonHeaders[0].keySequence;
for (int i = 1; i < commonHeaders.size(); i++) {
if (commonHeaders[i].magicNumber != magicN) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Magic numbers do not match. " + filenames[0] + " magic number is " + toString(commonHeaders[0].magicNumber) + ", but " + filenames[i] + " magic number is " + toString(commonHeaders[i].magicNumber) + ".\n"); }
if (commonHeaders[i].version != version) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Versions do not match. " + filenames[0] + " version is " + commonHeaders[0].version + ", but " + filenames[i] + " version is " + commonHeaders[i].version + ".\n"); }
if (commonHeaders[i].headerLength != headerLength) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Header lengths do not match. " + filenames[0] + " header length is " + toString(commonHeaders[0].headerLength) + ", but " + filenames[i] + " header length is " + toString(commonHeaders[i].headerLength) + ".\n"); }
if (commonHeaders[i].keyLength != keyLength) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Key Lengths do not match. " + filenames[0] + " Key length is " + toString(commonHeaders[0].keyLength) + ", but " + filenames[i] + " key length is " + toString(commonHeaders[i].keyLength) + ".\n"); }
if (commonHeaders[i].numFlowsPerRead != numFlows) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Number of flows per read do not match. " + filenames[0] + " number of flows is " + toString(commonHeaders[0].numFlowsPerRead) + ", but " + filenames[i] + " number of flows is " + toString(commonHeaders[i].numFlowsPerRead) + ".\n"); }
if (commonHeaders[i].flogramFormatCode != flowCode) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Flow format codes do not match. " + filenames[0] + " Flow format code is " + toString(commonHeaders[0].flogramFormatCode) + ", but " + filenames[i] + " flow format code is " + toString(commonHeaders[i].flogramFormatCode) + ".\n"); }
if (commonHeaders[i].flowChars != flowChars) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Flow characters do not match. " + filenames[0] + " Flow characters are " + commonHeaders[0].flowChars + ", but " + filenames[i] + " flow characters are " + commonHeaders[i].flowChars + ".\n"); }
if (commonHeaders[i].keySequence != keySeq) { okay = false; m->mothurOut("[ERROR]: merge issue with common headers. Key sequences do not match. " + filenames[0] + " Key sequence is " + commonHeaders[0].keySequence + ", but " + filenames[i] + " key sequence is " + commonHeaders[i].keySequence + ".\n"); }
}
}else { m->control_pressed = true; return 0; } //should never get here
if (!okay) { m->control_pressed = true; return 0; }
string endian = m->findEdianness();
char* mybuffer = new char[4];
ifstream in;
m->openInputFileBinary(currentFileName, in);
//magic number
in.read(mybuffer,4);
ofstream out;
m->openOutputFileBinaryAppend(outputFileHeader, out);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
//version
mybuffer = new char[4];
in.read(mybuffer,4);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
//offset
mybuffer = new char[8];
in.read(mybuffer,8);
unsigned long long offset = 0;
char* thisbuffer = new char[8];
thisbuffer[0] = (offset >> 56) & 0xFF;
thisbuffer[1] = (offset >> 48) & 0xFF;
thisbuffer[2] = (offset >> 40) & 0xFF;
thisbuffer[3] = (offset >> 32) & 0xFF;
thisbuffer[4] = (offset >> 24) & 0xFF;
thisbuffer[5] = (offset >> 16) & 0xFF;
thisbuffer[6] = (offset >> 8) & 0xFF;
thisbuffer[7] = offset & 0xFF;
out.write(thisbuffer, 8);
delete[] thisbuffer;
delete[] mybuffer;
//read index length
mybuffer = new char[4];
in.read(mybuffer,4);
offset = 0;
char* thisbuffer2 = new char[4];
thisbuffer2[0] = (offset >> 24) & 0xFF;
thisbuffer2[1] = (offset >> 16) & 0xFF;
thisbuffer2[2] = (offset >> 8) & 0xFF;
thisbuffer2[3] = offset & 0xFF;
out.write(thisbuffer2, 4);
delete[] thisbuffer2;
delete[] mybuffer;
//change num reads
mybuffer = new char[4];
in.read(mybuffer,4);
delete[] mybuffer;
thisbuffer2 = new char[4];
if (endian == "BIG_ENDIAN") {
thisbuffer2[0] = (numTotalReads >> 24) & 0xFF;
thisbuffer2[1] = (numTotalReads >> 16) & 0xFF;
thisbuffer2[2] = (numTotalReads >> 8) & 0xFF;
thisbuffer2[3] = numTotalReads & 0xFF;
}else {
thisbuffer2[0] = numTotalReads & 0xFF;
thisbuffer2[1] = (numTotalReads >> 8) & 0xFF;
thisbuffer2[2] = (numTotalReads >> 16) & 0xFF;
thisbuffer2[3] = (numTotalReads >> 24) & 0xFF;
}
out.write(thisbuffer2, 4);
delete[] thisbuffer2;
//read header length
mybuffer = new char[2];
in.read(mybuffer,2);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
//read key length
mybuffer = new char[2];
in.read(mybuffer,2);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
//read number of flow reads
mybuffer = new char[2];
in.read(mybuffer,2);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
//read format code
mybuffer = new char[1];
in.read(mybuffer,1);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
//read flow chars
mybuffer = new char[commonHeaders[0].numFlowsPerRead];
in.read(mybuffer,commonHeaders[0].numFlowsPerRead);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
//read key
mybuffer = new char[commonHeaders[0].keyLength];
in.read(mybuffer,commonHeaders[0].keyLength);
out.write(mybuffer, in.gcount());
delete[] mybuffer;
/* Pad to 8 chars */
unsigned long long spotInFile = in.tellg();
unsigned long long spot = (spotInFile + 7)& ~7; // ~ inverts
in.seekg(spot);
mybuffer = new char[spot-spotInFile];
out.write(mybuffer, spot-spotInFile);
delete[] mybuffer;
in.close();
out.close();
m->appendBinaryFiles(outputFile, outputFileHeader);
m->renameFile(outputFileHeader, outputFile);
m->mothurRemove(outputFileHeader);
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "adjustCommonHeader");
exit(1);
}
}
//**********************************************************************************************************************
bool MergeSfffilesCommand::readSeqData(ifstream& in, seqRead& read, int numFlowReads, Header& header, ofstream& out){
try {
unsigned long long startSpotInFile = in.tellg();
if (!in.eof()) {
/*****************************************/
//read header
//read header length
char buffer [2];
in.read(buffer, 2);
header.headerLength = be_int2(*(unsigned short *)(&buffer));
//read name length
char buffer2 [2];
in.read(buffer2, 2);
header.nameLength = be_int2(*(unsigned short *)(&buffer2));
//read num bases
char buffer3 [4];
in.read(buffer3, 4);
header.numBases = be_int4(*(unsigned int *)(&buffer3));
//read clip qual left
char buffer4 [2];
in.read(buffer4, 2);
header.clipQualLeft = be_int2(*(unsigned short *)(&buffer4));
header.clipQualLeft = 5;
//read clip qual right
char buffer5 [2];
in.read(buffer5, 2);
header.clipQualRight = be_int2(*(unsigned short *)(&buffer5));
//read clipAdapterLeft
char buffer6 [2];
in.read(buffer6, 2);
header.clipAdapterLeft = be_int2(*(unsigned short *)(&buffer6));
//read clipAdapterRight
char buffer7 [2];
in.read(buffer7, 2);
header.clipAdapterRight = be_int2(*(unsigned short *)(&buffer7));
//read name
char* tempBuffer = new char[header.nameLength];
in.read(&(*tempBuffer), header.nameLength);
header.name = tempBuffer;
if (header.name.length() > header.nameLength) { header.name = header.name.substr(0, header.nameLength); }
delete[] tempBuffer;
/* Pad to 8 chars */
unsigned long long spotInFile = in.tellg();
unsigned long long spot = (spotInFile + 7)& ~7;
in.seekg(spot);
/*****************************************/
//sequence read
//read flowgram
read.flowgram.resize(numFlowReads);
for (int i = 0; i < numFlowReads; i++) {
char buffer [2];
in.read(buffer, 2);
read.flowgram[i] = be_int2(*(unsigned short *)(&buffer));
}
//read flowIndex
read.flowIndex.resize(header.numBases);
for (int i = 0; i < header.numBases; i++) {
char temp[1];
in.read(temp, 1);
read.flowIndex[i] = be_int1(*(unsigned char *)(&temp));
}
//read bases
char* tempBuffer6 = new char[header.numBases];
in.read(&(*tempBuffer6), header.numBases);
read.bases = tempBuffer6;
if (read.bases.length() > header.numBases) { read.bases = read.bases.substr(0, header.numBases); }
delete[] tempBuffer6;
//read qual scores
read.qualScores.resize(header.numBases);
for (int i = 0; i < header.numBases; i++) {
char temp[1];
in.read(temp, 1);
read.qualScores[i] = be_int1(*(unsigned char *)(&temp));
}
/* Pad to 8 chars */
spotInFile = in.tellg();
spot = (spotInFile + 7)& ~7;
in.seekg(spot);
char * mybuffer;
mybuffer = new char [spot-startSpotInFile];
ifstream in2;
m->openInputFileBinary(currentFileName, in2);
in2.seekg(startSpotInFile);
in2.read(mybuffer,spot-startSpotInFile);
out.write(mybuffer, in2.gcount());
numTotalReads++;
delete[] mybuffer;
in2.close();
}else{
m->mothurOut("Error reading."); m->mothurOutEndLine();
}
if (in.eof()) { return true; }
return false;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "readSeqData");
exit(1);
}
}
//**********************************************************************************************************************
bool MergeSfffilesCommand::sanityCheck(Header& header, seqRead& read) {
try {
bool okay = true;
string message = "[WARNING]: Your sff file may be corrupted! Sequence: " + header.name + "\n";
if (header.clipQualLeft > read.bases.length()) {
okay = false; message += "Clip Qual Left = " + toString(header.clipQualLeft) + ", but we only read " + toString(read.bases.length()) + " bases.\n";
}
if (header.clipQualRight > read.bases.length()) {
okay = false; message += "Clip Qual Right = " + toString(header.clipQualRight) + ", but we only read " + toString(read.bases.length()) + " bases.\n";
}
if (header.clipQualLeft > read.qualScores.size()) {
okay = false; message += "Clip Qual Left = " + toString(header.clipQualLeft) + ", but we only read " + toString(read.qualScores.size()) + " quality scores.\n";
}
if (header.clipQualRight > read.qualScores.size()) {
okay = false; message += "Clip Qual Right = " + toString(header.clipQualRight) + ", but we only read " + toString(read.qualScores.size()) + " quality scores.\n";
}
if (okay == false) {
m->mothurOut(message); m->mothurOutEndLine();
}
return okay;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "sanityCheck");
exit(1);
}
}
//**********************************************************************************************************************
int MergeSfffilesCommand::readFile(){
try {
string filename;
ifstream in;
m->openInputFile(file, in);
while(!in.eof()) {
if (m->control_pressed) { return 0; }
in >> filename; m->gobble(in);
if (m->debug) { m->mothurOut("[DEBUG]: filename = " + filename + ".\n"); }
//check to make sure both are able to be opened
ifstream in2;
int openForward = m->openInputFile(filename, in2, "noerror");
//if you can't open it, try default location
if (openForward == 1) {
if (m->getDefaultPath() != "") { //default path is set
string tryPath = m->getDefaultPath() + m->getSimpleName(filename);
m->mothurOut("Unable to open " + filename + ". Trying default " + tryPath); m->mothurOutEndLine();
ifstream in3;
openForward = m->openInputFile(tryPath, in3, "noerror");
in3.close();
filename = tryPath;
}
}
//if you can't open it, try output location
if (openForward == 1) {
if (m->getOutputDir() != "") { //default path is set
string tryPath = m->getOutputDir() + m->getSimpleName(filename);
m->mothurOut("Unable to open " + filename + ". Trying output directory " + tryPath); m->mothurOutEndLine();
ifstream in4;
openForward = m->openInputFile(tryPath, in4, "noerror");
filename = tryPath;
in4.close();
}
}
if (openForward == 1) { //can't find it
m->mothurOut("[WARNING]: can't find " + filename + ", ignoring.\n");
}else{ filenames.push_back(filename); }
}
in.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "MergeSfffilesCommand", "readFileNames");
exit(1);
}
}
//**********************************************************************************************************************
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