1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219
|
/*
* File: kruskalwalliscommand.cpp
* Author: kiverson
*
* Created on June 26, 2012, 11:06 AM
*/
#include "kruskalwalliscommand.h"
#include "linearalgebra.h"
//**********************************************************************************************************************
vector<string> KruskalWallisCommand::setParameters(){
try {
CommandParameter pdesign("design", "InputTypes", "", "", "none", "none", "none","",false,true,true); parameters.push_back(pdesign);
CommandParameter pshared("shared", "InputTypes", "", "", "none", "none", "none","summary",false,true,true); parameters.push_back(pshared);
CommandParameter pclass("class", "String", "", "", "", "", "","",false,false); parameters.push_back(pclass);
CommandParameter plabel("label", "String", "", "", "", "", "","",false,false); parameters.push_back(plabel);
//every command must have inputdir and outputdir. This allows mothur users to redirect input and output files.
CommandParameter pseed("seed", "Number", "", "0", "", "", "","",false,false); parameters.push_back(pseed);
CommandParameter pinputdir("inputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(pinputdir);
CommandParameter poutputdir("outputdir", "String", "", "", "", "", "","",false,false); parameters.push_back(poutputdir);
abort = false; calledHelp = false; allLines = true;
vector<string> tempOutNames;
outputTypes["kruskall-wallis"] = tempOutNames;
vector<string> myArray;
for (int i = 0; i < parameters.size(); i++) { myArray.push_back(parameters[i].name); }
return myArray;
}
catch(exception& e) {
m->errorOut(e, "KruskalWallisCommand", "setParameters");
exit(1);
}
}
//**********************************************************************************************************************
string KruskalWallisCommand::getHelpString(){
try {
string helpString = "";
helpString += "The kruskal.wallis command allows you to ....\n";
helpString += "The kruskal.wallis command parameters are: shared, design, class, label and classes.\n";
helpString += "The class parameter is used to indicate the which category you would like used for the Kruskal Wallis analysis. If none is provided first category is used.\n";
helpString += "The label parameter is used to indicate which distances in the shared file you would like to use. labels are separated by dashes.\n";
helpString += "The kruskal.wallis command should be in the following format: kruskal.wallis(shared=final.an.shared, design=final.design, class=treatment).\n";
return helpString;
}
catch(exception& e) {
m->errorOut(e, "KruskalWallisCommand", "getHelpString");
exit(1);
}
}
//**********************************************************************************************************************
string KruskalWallisCommand::getOutputPattern(string type) {
try {
string pattern = "";
if (type == "kruskall-wallis") { pattern = "[filename],[distance],kruskall_wallis"; }
else { m->mothurOut("[ERROR]: No definition for type " + type + " output pattern.\n"); m->setControl_pressed(true); }
return pattern;
}
catch(exception& e) {
m->errorOut(e, "KruskalWallisCommand", "getOutputPattern");
exit(1);
}
}
//**********************************************************************************************************************
KruskalWallisCommand::KruskalWallisCommand(string option) : Command() {
try {
if(option == "help") { help(); abort = true; calledHelp = true; }
else if(option == "citation") { citation(); abort = true; calledHelp = true;}
else if(option == "category") { abort = true; calledHelp = true; }
else {
OptionParser parser(option, setParameters());
map<string,string> parameters = parser.getParameters();
ValidParameters validParameter;
sharedfile = validParameter.validFile(parameters, "shared");
if (sharedfile == "not open") { sharedfile = ""; abort = true; }
else if (sharedfile == "not found") {
//if there is a current shared file, use it
sharedfile = current->getSharedFile();
if (sharedfile != "") { m->mothurOut("Using " + sharedfile + " as input file for the shared parameter.\n"); }
else { m->mothurOut("You have no current sharedfile and the shared parameter is required.\n"); abort = true; }
}else { current->setSharedFile(sharedfile); }
//get shared file, it is required
designfile = validParameter.validFile(parameters, "design");
if (designfile == "not open") { designfile = ""; abort = true; }
else if (designfile == "not found") {
//if there is a current shared file, use it
designfile = current->getDesignFile();
if (designfile != "") { m->mothurOut("Using " + designfile + " as input file for the design parameter.\n"); }
else { m->mothurOut("You have no current design file and the design parameter is required.\n"); abort = true; }
}else { current->setDesignFile(designfile); }
if (outputdir == ""){
outputdir = util.hasPath(sharedfile);
}
string label = validParameter.valid(parameters, "label");
if (label == "not found") { label = ""; }
else {
if(label != "all") { util.splitAtDash(label, labels); allLines = false; }
else { allLines = true; }
}
mclass = validParameter.valid(parameters, "class");
if (mclass == "not found") { mclass = ""; }
}
}
catch(exception& e) {
m->errorOut(e, "KruskalWallisCommand", "KruskalWallisCommand");
exit(1);
}
}
//**********************************************************************************************************************
int KruskalWallisCommand::execute(){
try {
if (abort) { if (calledHelp) { return 0; } return 2; }
DesignMap designMap(designfile); if (m->getControl_pressed()) { return 0; }
//if user did not select class use first column
if (mclass == "") { mclass = designMap.getDefaultClass(); m->mothurOut("\nYou did not provide a class, using " + mclass +".\n\n"); }
InputData input(sharedfile, "sharedfile", nullVector);
set<string> processedLabels;
set<string> userLabels = labels;
string lastLabel = "";
SharedRAbundVectors* lookup = util.getNextShared(input, allLines, userLabels, processedLabels, lastLabel);
vector<string> currentLabels = lookup->getOTUNames();
while (lookup != nullptr) {
if (m->getControl_pressed()) { delete lookup; break; }
vector<SharedRAbundVector*> data = lookup->getSharedRAbundVectors();
process(data, designMap, currentLabels);
for (int i = 0; i < data.size(); i++) { delete data[i]; } data.clear();
delete lookup;
lookup = util.getNextShared(input, allLines, userLabels, processedLabels, lastLabel);
}
//output files created by command
m->mothurOut("\nOutput File Names: \n");
for (int i = 0; i < outputNames.size(); i++) { m->mothurOut(outputNames[i] +"\n"); } m->mothurOutEndLine();
return 0;
}
catch(exception& e) {
m->errorOut(e, "KruskalWallisCommand", "execute");
exit(1);
}
}
//**********************************************************************************************************************
int KruskalWallisCommand::process(vector<SharedRAbundVector*>& lookup, DesignMap& designMap, vector<string> currentLabels) {
try {
map<string, string> variables;
variables["[filename]"] = outputdir + util.getRootName(util.getSimpleName(sharedfile));
variables["[distance]"] = lookup[0]->getLabel();
string outputFileName = getOutputFileName("kruskall-wallis",variables);
ofstream out;
util.openOutputFile(outputFileName, out);
outputNames.push_back(outputFileName); outputTypes["kruskall-wallis"].push_back(outputFileName);
out << "OTULabel\tKW\tPvalue\n";
int numBins = lookup[0]->getNumBins();
//sanity check to make sure each treatment has a group in the shared file
set<string> treatments;
for (int j = 0; j < lookup.size(); j++) {
string group = lookup[j]->getGroup();
string treatment = designMap.get(group, mclass); //get value for this group in this category
treatments.insert(treatment);
}
if (treatments.size() < 2) { m->mothurOut("[ERROR]: need at least 2 things for classes to compare, quitting.\n"); m->setControl_pressed(true); }
LinearAlgebra linear;
for (int i = 0; i < numBins; i++) {
if (m->getControl_pressed()) { break; }
vector<spearmanRank> values;
for (int j = 0; j < lookup.size(); j++) {
string group = lookup[j]->getGroup();
string treatment = designMap.get(group, mclass); //get value for this group in this category
spearmanRank temp(treatment, lookup[j]->get(i));
values.push_back(temp);
}
double pValue = 0.0;
double H = linear.calcKruskalWallis(values, pValue);
//output H and signifigance
out << currentLabels[i] << '\t' << H << '\t' << pValue << endl;
}
out.close();
return 0;
}
catch(exception& e) {
m->errorOut(e, "KruskalWallisCommand", "process");
exit(1);
}
}
//**********************************************************************************************************************
|