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#include "muscle.h"
#include <math.h>
#include "pwpath.h"
#include "profile.h"
#include <stdio.h>
// Textbook Smith-Waterman affine gap implementation.
#define TRACE 0
static const char *LocalScoreToStr(SCORE s)
{
static char str[16];
if (MINUS_INFINITY == s)
return " *";
sprintf(str, "%6.2f", s);
return str;
}
static void ListDP(const SCORE *DPM_, const ProfPos *PA, const ProfPos *PB,
unsigned uPrefixCountA, unsigned uPrefixCountB)
{
Log(" ");
for (unsigned uPrefixLengthB = 0; uPrefixLengthB < uPrefixCountB; ++uPrefixLengthB)
{
char c = ' ';
if (uPrefixLengthB > 0)
c = ConsensusChar(PB[uPrefixLengthB - 1]);
Log(" %4u:%c", uPrefixLengthB, c);
}
Log("\n");
for (unsigned uPrefixLengthA = 0; uPrefixLengthA < uPrefixCountA; ++uPrefixLengthA)
{
char c = ' ';
if (uPrefixLengthA > 0)
c = ConsensusChar(PA[uPrefixLengthA - 1]);
Log("%4u:%c ", uPrefixLengthA, c);
for (unsigned uPrefixLengthB = 0; uPrefixLengthB < uPrefixCountB; ++uPrefixLengthB)
Log(" %s", LocalScoreToStr(DPM(uPrefixLengthA, uPrefixLengthB)));
Log("\n");
}
}
SCORE SW(const ProfPos *PA, unsigned uLengthA, const ProfPos *PB,
unsigned uLengthB, PWPath &Path)
{
assert(uLengthB > 0 && uLengthA > 0);
const unsigned uPrefixCountA = uLengthA + 1;
const unsigned uPrefixCountB = uLengthB + 1;
// Allocate DP matrices
const size_t LM = uPrefixCountA*uPrefixCountB;
SCORE *DPM_ = new SCORE[LM];
SCORE *DPD_ = new SCORE[LM];
SCORE *DPI_ = new SCORE[LM];
DPM(0, 0) = 0;
DPD(0, 0) = MINUS_INFINITY;
DPI(0, 0) = MINUS_INFINITY;
DPM(1, 0) = MINUS_INFINITY;
DPD(1, 0) = MINUS_INFINITY;
DPI(1, 0) = MINUS_INFINITY;
DPM(0, 1) = MINUS_INFINITY;
DPD(0, 1) = MINUS_INFINITY;
DPI(0, 1) = MINUS_INFINITY;
// Empty prefix of B is special case
for (unsigned uPrefixLengthA = 2; uPrefixLengthA < uPrefixCountA; ++uPrefixLengthA)
{
// M=LetterA+LetterB, impossible with empty prefix
DPM(uPrefixLengthA, 0) = MINUS_INFINITY;
// D=LetterA+GapB, never optimal in local alignment with gap penalties
DPD(uPrefixLengthA, 0) = MINUS_INFINITY;
// I=GapA+LetterB, impossible with empty prefix
DPI(uPrefixLengthA, 0) = MINUS_INFINITY;
}
// Empty prefix of A is special case
for (unsigned uPrefixLengthB = 2; uPrefixLengthB < uPrefixCountB; ++uPrefixLengthB)
{
// M=LetterA+LetterB, impossible with empty prefix
DPM(0, uPrefixLengthB) = MINUS_INFINITY;
// D=LetterA+GapB, impossible with empty prefix
DPD(0, uPrefixLengthB) = MINUS_INFINITY;
// I=GapA+LetterB, never optimal in local alignment with gap penalties
DPI(0, uPrefixLengthB) = MINUS_INFINITY;
}
SCORE scoreMax = MINUS_INFINITY;
unsigned uPrefixLengthAMax = uInsane;
unsigned uPrefixLengthBMax = uInsane;
// ============
// Main DP loop
// ============
SCORE scoreGapCloseB = MINUS_INFINITY;
for (unsigned uPrefixLengthB = 1; uPrefixLengthB < uPrefixCountB; ++uPrefixLengthB)
{
const ProfPos &PPB = PB[uPrefixLengthB - 1];
SCORE scoreGapCloseA = MINUS_INFINITY;
for (unsigned uPrefixLengthA = 1; uPrefixLengthA < uPrefixCountA; ++uPrefixLengthA)
{
const ProfPos &PPA = PA[uPrefixLengthA - 1];
{
// Match M=LetterA+LetterB
SCORE scoreLL = ScoreProfPos2(PPA, PPB);
SCORE scoreMM = DPM(uPrefixLengthA-1, uPrefixLengthB-1);
SCORE scoreDM = DPD(uPrefixLengthA-1, uPrefixLengthB-1) + scoreGapCloseA;
SCORE scoreIM = DPI(uPrefixLengthA-1, uPrefixLengthB-1) + scoreGapCloseB;
SCORE scoreBest;
if (scoreMM >= scoreDM && scoreMM >= scoreIM)
scoreBest = scoreMM;
else if (scoreDM >= scoreMM && scoreDM >= scoreIM)
scoreBest = scoreDM;
else
{
assert(scoreIM >= scoreMM && scoreIM >= scoreDM);
scoreBest = scoreIM;
}
if (scoreBest < 0)
scoreBest = 0;
scoreBest += scoreLL;
if (scoreBest > scoreMax)
{
scoreMax = scoreBest;
uPrefixLengthAMax = uPrefixLengthA;
uPrefixLengthBMax = uPrefixLengthB;
}
DPM(uPrefixLengthA, uPrefixLengthB) = scoreBest;
}
{
// Delete D=LetterA+GapB
SCORE scoreMD = DPM(uPrefixLengthA-1, uPrefixLengthB) +
PA[uPrefixLengthA-1].m_scoreGapOpen;
SCORE scoreDD = DPD(uPrefixLengthA-1, uPrefixLengthB);
SCORE scoreBest;
if (scoreMD >= scoreDD)
scoreBest = scoreMD;
else
{
assert(scoreDD >= scoreMD);
scoreBest = scoreDD;
}
DPD(uPrefixLengthA, uPrefixLengthB) = scoreBest;
}
// Insert I=GapA+LetterB
{
SCORE scoreMI = DPM(uPrefixLengthA, uPrefixLengthB-1) +
PB[uPrefixLengthB - 1].m_scoreGapOpen;
SCORE scoreII = DPI(uPrefixLengthA, uPrefixLengthB-1);
SCORE scoreBest;
if (scoreMI >= scoreII)
scoreBest = scoreMI;
else
{
assert(scoreII > scoreMI);
scoreBest = scoreII;
}
DPI(uPrefixLengthA, uPrefixLengthB) = scoreBest;
}
scoreGapCloseA = PPA.m_scoreGapClose;
}
scoreGapCloseB = PPB.m_scoreGapClose;
}
#if TRACE
Log("DPM:\n");
ListDP(DPM_, PA, PB, uPrefixLengthA, uPrefixLengthB);
Log("DPD:\n");
ListDP(DPD_, PA, PB, uPrefixLengthA, uPrefixLengthB);
Log("DPI:\n");
ListDP(DPI_, PA, PB, uPrefixLengthA, uPrefixLengthB);
#endif
assert(scoreMax == DPM(uPrefixLengthAMax, uPrefixLengthBMax));
TraceBackSW(PA, uLengthA, PB, uLengthB, DPM_, DPD_, DPI_,
uPrefixLengthAMax, uPrefixLengthBMax, Path);
#if TRACE
SCORE scorePath = FastScorePath2(PA, uLengthA, PB, uLengthB, Path);
Path.LogMe();
Log("Score = %s Path = %s\n", LocalScoreToStr(scoreMax), LocalScoreToStr(scorePath));
#endif
delete[] DPM_;
delete[] DPD_;
delete[] DPI_;
return scoreMax;
}
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