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/*****************************************************************************
*
* asn2fast.c
*
*****************************************************************************/
#include <tofasta.h>
#include <subutil.h>
#include <sqnutils.h>
#include <accid1.h>
#include <lsqfetch.h>
#define NUMARG 18
Args myargs[NUMARG] = {
{"Filename for asn.1 input","stdin",NULL,NULL,TRUE,'a',ARG_FILE_IN,0.0,0,NULL},
{"Input is a Seq-entry","F", NULL ,NULL ,TRUE,'e',ARG_BOOLEAN,0.0,0,NULL},
{"Input asnfile in binary mode","F",NULL,NULL,TRUE,'b',ARG_BOOLEAN,0.0,0,NULL},
{"Output Protein Filename","fasta.aa", NULL,NULL,TRUE,'p',ARG_FILE_OUT,0.0,0,NULL},
{"Output DNA Filename","fasta.na", NULL,NULL,TRUE,'n',ARG_FILE_OUT,0.0,0,NULL},
{"Log errors to file named:",NULL,NULL,NULL,TRUE,'l',ARG_FILE_OUT, 0.0,0,NULL},
{"Combine segmented or delta sequences","F",NULL,NULL,TRUE,'c',ARG_BOOLEAN,0.0,0,NULL},
{"Produce Protein File","T",NULL,NULL,TRUE,'x',ARG_BOOLEAN,0.0,0,NULL},
{"Produce DNA File","T",NULL,NULL,TRUE,'d',ARG_BOOLEAN,0.0,0,NULL},
{"Limit to GenBank","F",NULL,NULL,TRUE,'g',ARG_BOOLEAN,0.0,0,NULL},
{"Instantiate virtual sequences","F",NULL,NULL,TRUE,'v',ARG_BOOLEAN,0.0,0,NULL},
{"Input is a Seq-submit","F", NULL ,NULL ,TRUE,'s',ARG_BOOLEAN,0.0,0,NULL},
{"Produce output file of Quality Scores (DNA sequences only)","F",NULL,NULL,TRUE,'q',ARG_BOOLEAN,0.0,0,NULL},
{"Output Filename for Quality Scores (DNA sequences only)","scores.ql", NULL,NULL,TRUE,'y',ARG_FILE_OUT,0.0,0,NULL},
{"Far Genomic Contig function for Quality Scores","F",NULL,NULL,TRUE,'f',ARG_BOOLEAN,0.0,0,NULL},
{"Remote fetching", "F", NULL, NULL, FALSE, 'r', ARG_BOOLEAN, 0.0, 0, NULL},
{"Local fetching", "F", NULL, NULL, FALSE, 'k', ARG_BOOLEAN, 0.0, 0, NULL},
{"Print Quality Score Gap as -1, false prints as 0", "F", NULL, NULL, FALSE, 'z', ARG_BOOLEAN, 0.0, 0, NULL},
};
static void PrintQualProc (CharPtr buf, Uint4 buflen, Pointer userdata)
{
FILE *fp;
fp = (FILE*) userdata;
fprintf (fp, "%s", buf);
}
static void PrintQualScores (SeqEntryPtr sep, Pointer data, Int4 index, Int2 indent)
{
BioseqPtr bsp;
FILE *fp;
if (IS_Bioseq (sep)) {
bsp = (BioseqPtr) sep->data.ptrvalue;
/* WARNING: we're assuming here that asn2fast's quality-score
output is DNA-centric, thus protein bioseqs can be ignored
in the PrintQualScores callback. --MLC, 5/2000 */
if (ISA_aa(bsp->mol))
return;
fp = (FILE*) data;
if (myargs [17].intvalue) {
PrintQualityScoresToBuffer (bsp, FALSE, fp, PrintQualProc);
} else {
PrintQualityScoresToBuffer (bsp, TRUE, fp, PrintQualProc);
}
}
}
static void PrintFarQualScores (SeqEntryPtr sep, Pointer data, Int4 index, Int2 indent)
{
BioseqPtr bsp;
FILE *fp;
if (IS_Bioseq (sep)) {
bsp = (BioseqPtr) sep->data.ptrvalue;
/* WARNING: we're assuming here that asn2fast's quality-score
output is DNA-centric, thus protein bioseqs can be ignored
in the PrintQualScores callback. --MLC, 5/2000 */
if (ISA_aa(bsp->mol))
return;
fp = (FILE*) data;
if (myargs [17].intvalue) {
PrintQualityScoresForContig (bsp, FALSE, fp);
} else {
PrintQualityScoresForContig (bsp, TRUE, fp);
}
}
}
Boolean CheckIsGenBank(SeqEntryPtr sep);
Int2 Main(void)
{
AsnIoPtr aip;
FILE * aa = NULL, * na = NULL, * ql = NULL;
SeqEntryPtr sep;
SeqSubmitPtr ssp;
AsnTypePtr atp, atp2;
AsnModulePtr amp;
Uint1 group_segs = 0;
Boolean limit_to_genbank,
make_dna,
make_protein,
make_quality,
far_quality,
do_it;
/* check command line arguments */
if ( ! GetArgs("asn2fast",NUMARG, myargs))
return 1;
/* load the sequence alphabets */
/* (and sequence parse trees) */
if (! SeqEntryLoad())
{
ErrShow();
return 1;
}
/* get pointer to all loaded ASN.1 modules */
amp = AsnAllModPtr();
if (amp == NULL)
{
ErrShow();
return 1;
}
if (myargs[11].intvalue) {
if (! SubmitAsnLoad())
Message(MSG_FATAL, "Unable to load parse trees.");
atp2 = AsnFind("Seq-submit");
if (atp2 == NULL)
Message(MSG_FATAL, "Unable to find Seq-submit");
atp = AsnFind("Seq-submit");
if (atp == NULL)
Message(MSG_FATAL, "Unable to find Seq-submit");
} else {
atp = AsnFind("Bioseq-set"); /* get the initial type pointers */
if (atp == NULL)
{
ErrShow();
return 1;
}
atp2 = AsnFind("Bioseq-set.seq-set.E");
if (atp2 == NULL)
{
ErrShow();
return 1;
}
}
make_protein = (Boolean)(myargs[7].intvalue);
make_dna = (Boolean)(myargs[8].intvalue);
make_quality = (Boolean)(myargs[12].intvalue);
far_quality = (Boolean)(myargs[14].intvalue);
/* open the ASN.1 input file in the right mode */
if ((aip = AsnIoOpen (myargs[0].strvalue, myargs[2].intvalue?"rb":"r"))
== NULL)
{
ErrShow();
return 1;
}
/* open the output file */
if ((myargs[3].strvalue != NULL) && (make_protein))
{
if ( (aa = FileOpen (myargs[3].strvalue, "w")) == NULL)
{
ErrShow();
return 1;
}
}
if ((myargs[4].strvalue != NULL) && (make_dna))
{
if ( (na = FileOpen (myargs[4].strvalue, "w")) == NULL)
{
ErrShow();
return 1;
}
}
if ((myargs[13].strvalue != NULL) && (make_quality))
{
if ( (ql = FileOpen (myargs[13].strvalue, "w")) == NULL)
{
ErrShow();
return 1;
}
}
/* log errors instead of die */
if (myargs[5].strvalue != NULL)
{
if (! ErrSetLog (myargs[5].strvalue))
ErrShow();
else
ErrSetOpts (ERR_CONTINUE, ERR_LOG_ON);
}
if (myargs[6].intvalue) /* combine segmented seqs */
{
group_segs = 1;
if (myargs[10].intvalue)
group_segs = 3; /* and instantiate virtuals */
}
limit_to_genbank = (Boolean)(myargs[9].intvalue);
if (myargs [15].intvalue) {
ID1BioseqFetchEnable ("asn2fast", FALSE);
}
if (myargs [16].intvalue) {
LocalSeqFetchInit (FALSE);
}
if ( myargs[1].intvalue) /* read one Seq-entry */
{
sep = SeqEntryAsnRead(aip, NULL);
do_it = TRUE;
if (limit_to_genbank)
do_it = CheckIsGenBank(sep);
if (do_it)
{
if (make_protein)
SeqEntrysToFasta(sep, aa, FALSE, group_segs);
if (make_dna)
SeqEntrysToFasta(sep, na, TRUE, group_segs);
if (make_quality) {
if (far_quality) {
SeqEntryExplore (sep, (Pointer) ql, PrintFarQualScores);
} else {
SeqEntryExplore (sep, (Pointer) ql, PrintQualScores);
}
}
}
SeqEntryFree(sep);
}
else if ( myargs[11].intvalue) /* read Seq-submit's */
{
while ((atp = AsnReadId(aip, amp, atp)) != NULL)
{
if (atp == atp2) /* top level Seq-entry */
{
ssp = SeqSubmitAsnRead(aip, atp);
if (ssp->datatype == 1)
{
sep = (SeqEntryPtr) ssp->data;
do_it = TRUE;
if (limit_to_genbank)
do_it = CheckIsGenBank(sep);
if (do_it)
{
if (make_protein)
SeqEntrysToFasta(sep, aa, FALSE, group_segs);
if (make_dna)
SeqEntrysToFasta(sep, na, TRUE, group_segs);
if (make_quality) {
if (far_quality) {
SeqEntryExplore (sep, (Pointer) ql, PrintFarQualScores);
} else {
SeqEntryExplore (sep, (Pointer) ql, PrintQualScores);
}
}
}
}
SeqSubmitFree(ssp);
}
else
{
AsnReadVal(aip, atp, NULL);
}
}
}
else /* read Seq-entry's from a Bioseq-set */
{
while ((atp = AsnReadId(aip, amp, atp)) != NULL)
{
if (atp == atp2) /* top level Seq-entry */
{
sep = SeqEntryAsnRead(aip, atp);
do_it = TRUE;
if (limit_to_genbank)
do_it = CheckIsGenBank(sep);
if (do_it)
{
if (make_protein)
SeqEntrysToFasta(sep, aa, FALSE, group_segs);
if (make_dna)
SeqEntrysToFasta(sep, na, TRUE, group_segs);
if (make_quality) {
if (far_quality) {
SeqEntryExplore (sep, (Pointer) ql, PrintFarQualScores);
} else {
SeqEntryExplore (sep, (Pointer) ql, PrintQualScores);
}
}
}
SeqEntryFree(sep);
}
else
{
AsnReadVal(aip, atp, NULL);
}
}
}
AsnIoClose(aip);
if (make_protein)
FileClose(aa);
if (make_dna)
FileClose(na);
if (make_quality)
FileClose (ql);
if (myargs [16].intvalue) {
LocalSeqFetchDisable ();
}
if (myargs [15].intvalue) {
ID1BioseqFetchDisable ();
}
return(0);
}
void FindGenBank (SeqEntryPtr sep, Pointer data, Int4 index, Int2 indent);
Boolean CheckIsGenBank(SeqEntryPtr sep)
{
Boolean retval = FALSE;
SeqEntryExplore(sep, (Pointer)(&retval), FindGenBank);
return retval;
}
void FindGenBank (SeqEntryPtr sep, Pointer data, Int4 index, Int2 indent)
{
BoolPtr ptr;
BioseqPtr bsp;
ValNodePtr vnp;
ptr = (BoolPtr)data;
if (*ptr == TRUE) /* already know */
return;
if (IS_Bioseq(sep))
{
bsp = (BioseqPtr)(sep->data.ptrvalue);
/* GenBank is a limited view of the world */
if ( (ISA_na(bsp->mol)) && ( (bsp->repr == Seq_repr_raw) || (bsp->repr == Seq_repr_delta) ) )
{
for (vnp = bsp->id; vnp != NULL; vnp = vnp->next)
{
switch (vnp->choice)
{
case SEQID_GENBANK:
case SEQID_EMBL:
case SEQID_DDBJ:
*ptr = TRUE;
return;
default:
break;
}
}
}
}
return;
}
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