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/*****************************************************************************
*
* seqtest.c
* test program for sequence display, SeqPort and ToFasta
*
*****************************************************************************/
#include <seqport.h>
#include <tofasta.h>
void BuildList (SeqEntryPtr sep, Pointer data, Int4 index, Int2 indent);
#define NUMARG 2
Args myargs[NUMARG] = {
{"Filename for asn.1 input","stdin",NULL,NULL,FALSE,'i',ARG_FILE_IN,0.0,0,NULL},
{"Filename for output","stdout", NULL,NULL,TRUE,'o',ARG_FILE_OUT,0.0,0,NULL}};
Int2 Main(void)
{
AsnIoPtr aip;
SeqEntryPtr sep;
BioseqPtr PNTR seqlist;
Int4 seqnum, i, numseg, lens[10], j;
Int2 ctr;
SeqPortPtr spp;
Uint1 residue;
FILE* fp;
CharPtr title;
Char buffer[101];
MonitorPtr mon;
/* check command line arguments */
if ( ! GetArgs("SeqTest",NUMARG, myargs))
return 1;
mon = MonitorStrNew("SeqTest", 40);
SetProgMon(StdProgMon, (Pointer)mon);
/*
** Load SeqEntry object loader and sequence alphabets
*/
if (! SeqEntryLoad()) {
Message(MSG_ERROR, "SeqEntryLoad failed");
return 1;
}
/*
** Use the file "example.prt" as the ASN I/O stream. This file
** can be found in the ncbi/demo. It is in ASN.1 Print Value format.
*/
if ((aip = AsnIoOpen(myargs[0].strvalue, "r")) == NULL)
return 1;
/*
** Write the output to "seqtest.out".
*/
fp = FileOpen(myargs[1].strvalue, "w");
fprintf(fp, "Sequence summary:\n\n");
/*
** Read in the whole entry into the Sequence Entry Pointer, sep.
** Close the ASN stream, which in turn closes the input file.
*/
sep = SeqEntryAsnRead(aip, NULL);
aip = AsnIoClose(aip);
mon = MonitorFree(mon);
SetProgMon(NULL, NULL);
/*
** Determine how many Bioseqs are in this SeqEntry. Allocate
** enough memory to hold a list of pointer to all of these
** Bioseqs. Invoke an Explore function to "visit"each Bioseq.
** We are allowed to pass one pointer for use by the exploring
** function, in this case, "BuildList".
*/
seqnum = BioseqCount(sep);
seqlist = MemNew((size_t)(seqnum * sizeof(BioseqPtr)));
BioseqExplore(sep, (Pointer) seqlist, BuildList);
/*
** For each Bioseq in the SeqEntry write out it's title
** len, number of gaps, and number of segments. Write out
** the length of each segment, up to 10.
*/
for(i = 0; i < seqnum; i++) {
numseg = BioseqCountSegs(seqlist[i]);
title = BioseqGetTitle(seqlist[i]);
FilePuts((VoidPtr)title, fp);
FilePuts("\n", fp);
fprintf(fp, "len=%d gaps=%d segs=%d\n", BioseqGetLen(seqlist[i]),
BioseqGetGaps(seqlist[i]), numseg);
if ((numseg > 1) && (numseg <= 10)) {
BioseqGetSegLens (seqlist[i], lens);
for (j = 0; j < numseg; j++)
fprintf(fp, " len = %d\n", lens[j]);
}
FilePuts("\n", fp);
}
spp = SeqPortNew(seqlist[0], 0, -1, 0, Seq_code_iupacna);
if (spp == NULL)
Message(MSG_ERROR, "fail on SeqPortNew");
fprintf(fp, "SeqPort: plus strand with SeqPortGetResidue\n\n");
i = 0;
while ((residue = SeqPortGetResidue(spp)) != SEQPORT_EOF) {
if (! IS_residue(residue)) {
buffer[i] = '\0';
fprintf(fp, "%s\n", buffer);
i = 0;
switch (residue)
{
case SEQPORT_VIRT:
fprintf(fp, "[Gap]\n");
break;
case SEQPORT_EOS:
fprintf(fp, "[EOS]\n");
break;
default:
fprintf(fp, "[Invalid Residue]\n");
break;
}
}
else {
buffer[i] = residue;
i++;
if (i == 60) {
buffer[i] = '\0';
fprintf(fp, "%s\n", buffer);
i = 0;
}
}
}
if (i) {
buffer[i] = '\0';
fprintf(fp, "%s\n", buffer);
}
fprintf(fp, "[EOF]\n");
SeqPortFree(spp);
fprintf(fp, "\nSeqPort on minus with SeqPortRead\n\n");
spp = SeqPortNew(seqlist[0], 0, -1, Seq_strand_minus, Seq_code_iupacna);
if (spp == NULL)
Message(MSG_ERROR, "fail on SeqPortNew");
do {
ctr = SeqPortRead(spp, (Uint1Ptr)buffer, 60);
if (ctr > 0) {
buffer[ctr] = '\0';
fprintf(fp, "%s\n", buffer);
} else {
ctr *= -1;
switch (ctr)
{
case SEQPORT_VIRT:
fprintf(fp, "[Gap]\n");
break;
case SEQPORT_EOS:
fprintf(fp, "[EOS]\n");
break;
case SEQPORT_EOF:
fprintf(fp, "[EOF]\n");
break;
default:
fprintf(fp, "[Invalid Residue]\n");
break;
}
}
} while (ctr != SEQPORT_EOF);
SeqPortFree(spp);
/*
** Write out the nucleic acid sequences in this SeqEntry
*/
fprintf(fp, "\nNucleic Acids in FASTA format:\n\n");
SeqEntryToFasta(sep, fp, TRUE);
/*
** Write out the protein sequences in this SeqEntry.
*/
fprintf(fp, "\nProteins in FASTA format:\n\n");
SeqEntryToFasta(sep, fp, FALSE);
/*
** Close the output file and free up allocated space.
*/
fclose(fp);
MemFree(seqlist);
SeqEntryFree(sep);
return 0;
}
/*
** This SeqEntry exploration function copy the current pointer position inthe
** the Bioseq entry to a list of Bioseq pointers
*/
void BuildList(SeqEntryPtr sep, Pointer data, Int4 index, Int2 indent)
{
((BioseqPtr PNTR) data)[index] = (BioseqPtr)sep->data.ptrvalue;
return;
}
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