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#!/usr/bin/env python
#
# deepzoom_server - Example web application for serving whole-slide images
#
# Copyright (c) 2010-2015 Carnegie Mellon University
# Copyright (c) 2023 Benjamin Gilbert
#
# This library is free software; you can redistribute it and/or modify it
# under the terms of version 2.1 of the GNU Lesser General Public License
# as published by the Free Software Foundation.
#
# This library is distributed in the hope that it will be useful, but
# WITHOUT ANY WARRANTY; without even the implied warranty of MERCHANTABILITY
# or FITNESS FOR A PARTICULAR PURPOSE. See the GNU Lesser General Public
# License for more details.
#
# You should have received a copy of the GNU Lesser General Public License
# along with this library; if not, write to the Free Software Foundation,
# Inc., 51 Franklin Street, Fifth Floor, Boston, MA 02110-1301 USA.
#
from __future__ import annotations
from argparse import ArgumentParser
import base64
from collections.abc import Callable
from io import BytesIO
import os
from pathlib import Path
import re
from typing import TYPE_CHECKING, Any, Literal, Mapping
from unicodedata import normalize
import zlib
from PIL import Image, ImageCms
from flask import Flask, Response, abort, make_response, render_template, url_for
if TYPE_CHECKING:
# Python 3.10+
from typing import TypeAlias
if os.name == 'nt':
_dll_path = os.getenv('OPENSLIDE_PATH')
if _dll_path is not None:
with os.add_dll_directory(_dll_path): # type: ignore[attr-defined,unused-ignore] # noqa: E501
import openslide
else:
import openslide
else:
import openslide
from openslide import AbstractSlide, ImageSlide, open_slide
from openslide.deepzoom import DeepZoomGenerator
SLIDE_NAME = 'slide'
# Optimized sRGB v2 profile, CC0-1.0 license
# https://github.com/saucecontrol/Compact-ICC-Profiles/blob/bdd84663/profiles/sRGB-v2-micro.icc
# ImageCms.createProfile() generates a v4 profile and Firefox has problems
# with those: https://littlecms.com/blog/2020/09/09/browser-check/
SRGB_PROFILE_BYTES = zlib.decompress(
base64.b64decode(
'eNpjYGA8kZOcW8wkwMCQm1dSFOTupBARGaXA/oiBmUGEgZOBj0E2Mbm4wDfYLYQBCIoT'
'y4uTS4pyGFDAt2sMjCD6sm5GYl7K3IkMtg4NG2wdSnQa5y1V6mPADzhTUouTgfQHII5P'
'LigqYWBg5AGyecpLCkBsCSBbpAjoKCBbB8ROh7AdQOwkCDsErCYkyBnIzgCyE9KR2ElI'
'bKhdIMBaCvQsskNKUitKQLSzswEDKAwgop9DwH5jFDuJEMtfwMBg8YmBgbkfIZY0jYFh'
'eycDg8QthJgKUB1/KwPDtiPJpUVlUGu0gLiG4QfjHKZS5maWk2x+HEJcEjxJfF8Ez4t8'
'k8iS0VNwVlmjmaVXZ/zacrP9NbdwX7OQshjxFNmcttKwut4OnUlmc1Yv79l0e9/MU8ev'
'pz4p//jz/38AR4Nk5Q=='
)
)
SRGB_PROFILE = ImageCms.getOpenProfile(BytesIO(SRGB_PROFILE_BYTES))
if TYPE_CHECKING:
ColorMode: TypeAlias = Literal[
'default',
'absolute-colorimetric',
'perceptual',
'relative-colorimetric',
'saturation',
'embed',
'ignore',
]
Transform: TypeAlias = Callable[[Image.Image], None]
class DeepZoomServer(Flask):
slides: dict[str, DeepZoomGenerator]
transforms: dict[str, Transform]
slide_properties: Mapping[str, str]
associated_images: list[str]
slide_mpp: float
def create_app(
config: dict[str, Any] | None = None,
config_file: Path | None = None,
) -> Flask:
# Create and configure app
app = DeepZoomServer(__name__)
app.config.from_mapping(
DEEPZOOM_SLIDE=None,
DEEPZOOM_FORMAT='jpeg',
DEEPZOOM_TILE_SIZE=254,
DEEPZOOM_OVERLAP=1,
DEEPZOOM_LIMIT_BOUNDS=True,
DEEPZOOM_TILE_QUALITY=75,
DEEPZOOM_COLOR_MODE='default',
)
app.config.from_envvar('DEEPZOOM_TILER_SETTINGS', silent=True)
if config_file is not None:
app.config.from_pyfile(config_file)
if config is not None:
app.config.from_mapping(config)
# Open slide
if app.config['DEEPZOOM_SLIDE'] is None:
raise ValueError('No slide file specified')
slidefile = Path(app.config['DEEPZOOM_SLIDE'])
config_map = {
'DEEPZOOM_TILE_SIZE': 'tile_size',
'DEEPZOOM_OVERLAP': 'overlap',
'DEEPZOOM_LIMIT_BOUNDS': 'limit_bounds',
}
opts = {v: app.config[k] for k, v in config_map.items()}
slide = open_slide(slidefile)
app.slides = {SLIDE_NAME: DeepZoomGenerator(slide, **opts)}
app.transforms = {
SLIDE_NAME: get_transform(slide, app.config['DEEPZOOM_COLOR_MODE'])
}
app.associated_images = []
app.slide_properties = slide.properties
for name, image in slide.associated_images.items():
app.associated_images.append(name)
slug = slugify(name)
image_slide = ImageSlide(image)
app.slides[slug] = DeepZoomGenerator(image_slide, **opts)
app.transforms[slug] = get_transform(
image_slide, app.config['DEEPZOOM_COLOR_MODE']
)
try:
mpp_x = slide.properties[openslide.PROPERTY_NAME_MPP_X]
mpp_y = slide.properties[openslide.PROPERTY_NAME_MPP_Y]
app.slide_mpp = (float(mpp_x) + float(mpp_y)) / 2
except (KeyError, ValueError):
app.slide_mpp = 0
# Set up routes
@app.route('/')
def index() -> str:
slide_url = url_for('dzi', slug=SLIDE_NAME)
associated_urls = {
name: url_for('dzi', slug=slugify(name)) for name in app.associated_images
}
return render_template(
'slide-multipane.html',
slide_url=slide_url,
associated=associated_urls,
properties=app.slide_properties,
slide_mpp=app.slide_mpp,
)
@app.route('/<slug>.dzi')
def dzi(slug: str) -> Response:
format = app.config['DEEPZOOM_FORMAT']
try:
resp = make_response(app.slides[slug].get_dzi(format))
resp.mimetype = 'application/xml'
return resp
except KeyError:
# Unknown slug
abort(404)
@app.route('/<slug>_files/<int:level>/<int:col>_<int:row>.<format>')
def tile(slug: str, level: int, col: int, row: int, format: str) -> Response:
format = format.lower()
if format != 'jpeg' and format != 'png':
# Not supported by Deep Zoom
abort(404)
try:
tile = app.slides[slug].get_tile(level, (col, row))
except KeyError:
# Unknown slug
abort(404)
except ValueError:
# Invalid level or coordinates
abort(404)
app.transforms[slug](tile)
buf = BytesIO()
tile.save(
buf,
format,
quality=app.config['DEEPZOOM_TILE_QUALITY'],
icc_profile=tile.info.get('icc_profile'),
)
resp = make_response(buf.getvalue())
resp.mimetype = 'image/%s' % format
return resp
return app
def slugify(text: str) -> str:
text = normalize('NFKD', text.lower()).encode('ascii', 'ignore').decode()
return re.sub('[^a-z0-9]+', '-', text)
def get_transform(image: AbstractSlide, mode: ColorMode) -> Transform:
if image.color_profile is None:
return lambda img: None
if mode == 'ignore':
# drop ICC profile from tiles
return lambda img: img.info.pop('icc_profile')
elif mode == 'embed':
# embed ICC profile in tiles
return lambda img: None
elif mode == 'default':
intent = ImageCms.Intent(ImageCms.getDefaultIntent(image.color_profile))
elif mode == 'absolute-colorimetric':
intent = ImageCms.Intent.ABSOLUTE_COLORIMETRIC
elif mode == 'relative-colorimetric':
intent = ImageCms.Intent.RELATIVE_COLORIMETRIC
elif mode == 'perceptual':
intent = ImageCms.Intent.PERCEPTUAL
elif mode == 'saturation':
intent = ImageCms.Intent.SATURATION
else:
raise ValueError(f'Unknown color mode {mode}')
transform = ImageCms.buildTransform(
image.color_profile,
SRGB_PROFILE,
'RGB',
'RGB',
intent,
ImageCms.Flags(0),
)
def xfrm(img: Image.Image) -> None:
ImageCms.applyTransform(img, transform, True)
# Some browsers assume we intend the display's color space if we don't
# embed the profile. Pillow's serialization is larger, so use ours.
img.info['icc_profile'] = SRGB_PROFILE_BYTES
return xfrm
if __name__ == '__main__':
parser = ArgumentParser(usage='%(prog)s [options] [SLIDE]')
parser.add_argument(
'-B',
'--ignore-bounds',
dest='DEEPZOOM_LIMIT_BOUNDS',
default=True,
action='store_false',
help='display entire scan area',
)
parser.add_argument(
'--color-mode',
dest='DEEPZOOM_COLOR_MODE',
choices=[
'default',
'absolute-colorimetric',
'perceptual',
'relative-colorimetric',
'saturation',
'embed',
'ignore',
],
default='default',
help=(
'convert tiles to sRGB using default rendering intent of ICC '
'profile, or specified rendering intent; or embed original '
'ICC profile; or ignore ICC profile (compat) [default]'
),
)
parser.add_argument(
'-c', '--config', metavar='FILE', type=Path, dest='config', help='config file'
)
parser.add_argument(
'-d',
'--debug',
dest='DEBUG',
action='store_true',
help='run in debugging mode (insecure)',
)
parser.add_argument(
'-e',
'--overlap',
metavar='PIXELS',
dest='DEEPZOOM_OVERLAP',
type=int,
help='overlap of adjacent tiles [1]',
)
parser.add_argument(
'-f',
'--format',
dest='DEEPZOOM_FORMAT',
choices=['jpeg', 'png'],
help='image format for tiles [jpeg]',
)
parser.add_argument(
'-l',
'--listen',
metavar='ADDRESS',
dest='host',
default='127.0.0.1',
help='address to listen on [127.0.0.1]',
)
parser.add_argument(
'-p',
'--port',
metavar='PORT',
dest='port',
type=int,
default=5000,
help='port to listen on [5000]',
)
parser.add_argument(
'-Q',
'--quality',
metavar='QUALITY',
dest='DEEPZOOM_TILE_QUALITY',
type=int,
help='JPEG compression quality [75]',
)
parser.add_argument(
'-s',
'--size',
metavar='PIXELS',
dest='DEEPZOOM_TILE_SIZE',
type=int,
help='tile size [254]',
)
parser.add_argument(
'DEEPZOOM_SLIDE',
metavar='SLIDE',
type=Path,
nargs='?',
help='slide file',
)
args = parser.parse_args()
config = {}
config_file = args.config
# Set only those settings specified on the command line
for k in dir(args):
v = getattr(args, k)
if not k.startswith('_') and v is not None:
config[k] = v
app = create_app(config, config_file)
app.run(host=args.host, port=args.port, threaded=True)
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