1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189
|
#include "PbbamInternalConfig.h"
#include <pbbam/BamReader.h>
#include <pbbam/BamRecord.h>
#include <pbbam/Deleters.h>
#include <pbbam/Validator.h>
#include "Autovalidate.h"
#include "MemoryUtils.h"
#include <htslib/bgzf.h>
#include <htslib/hfile.h>
#include <htslib/hts.h>
#include <htslib/thread_pool.h>
#include <optional>
#include <sstream>
#include <stdexcept>
#include <string>
#include <cassert>
#include <cstdint>
#include <cstdio>
#include <cstdlib>
namespace PacBio {
namespace BAM {
struct SamFileHandle
{
htsThreadPool ThreadPool = {NULL, 0};
samFile* File = nullptr;
~SamFileHandle()
{
if (File) {
sam_close(File);
File = nullptr;
}
if (ThreadPool.pool) {
hts_tpool_destroy(ThreadPool.pool);
ThreadPool.pool = nullptr;
}
}
};
class BamReader::BamReaderPrivate
{
public:
explicit BamReaderPrivate(std::string fn) : filename_{std::move(fn)}
{
auto displayFilename = [&]() {
if (filename_ == "-") {
return std::string{" stdin"};
} else {
return "\n file: " + filename_;
}
};
handle_.File = sam_open(filename_.c_str(), "rb");
if (!handle_.File || !handle_.File->fp.bgzf) {
std::ostringstream s;
s << "[pbbam] BAM reader ERROR: could not open for reading:" << displayFilename();
throw std::runtime_error{s.str()};
}
// Use environment variable as number of BamReader threads
if (const char* envCStr = std::getenv("PB_BAMREADER_THREADS")) {
try {
const int32_t numThreads = std::stoi(envCStr);
if (numThreads <= 0) {
std::ostringstream s;
s << "[pbbam] BAM reader ERROR: environment variable PB_BAMREADER_THREADS is "
"not a positive, non-negative number:"
<< envCStr;
throw std::runtime_error{s.str()};
}
handle_.ThreadPool.pool = hts_tpool_init(numThreads);
hts_set_opt(handle_.File, HTS_OPT_THREAD_POOL, &handle_.ThreadPool);
} catch (const std::exception&) {
std::ostringstream s;
s << "[pbbam] BAM reader ERROR: environment variable PB_BAMREADER_THREADS is not a "
"number:"
<< envCStr;
throw std::runtime_error{s.str()};
}
}
const auto bgzfPos = bgzf_tell(handle_.File->fp.bgzf);
if (bgzfPos != 0) {
std::ostringstream s;
s << "[pbbam] BAM reader ERROR: could not read from empty input:" << displayFilename();
throw std::runtime_error{s.str()};
}
const std::unique_ptr<bam_hdr_t, HtslibHeaderDeleter> hdr(sam_hdr_read(handle_.File));
if (!hdr) {
std::ostringstream s;
s << "[pbbam] BAM reader ERROR: could not read header from:" << displayFilename();
throw std::runtime_error{s.str()};
}
header_ = BamHeaderMemory::FromRawData(hdr.get());
}
std::string filename_;
SamFileHandle handle_;
BamHeader header_;
};
BamReader::BamReader() : internal::IQuery{}, d_{std::make_unique<BamReaderPrivate>("-")} {}
BamReader::BamReader(std::string fn)
: internal::IQuery{}, d_{std::make_unique<BamReaderPrivate>(std::move(fn))}
{}
BamReader::BamReader(BamFile bamFile) : BamReader{bamFile.Filename()} {}
BamReader::BamReader(BamReader&&) noexcept = default;
BamReader& BamReader::operator=(BamReader&&) noexcept = default;
BamReader::~BamReader() = default;
BGZF* BamReader::Bgzf() const { return d_->handle_.File->fp.bgzf; }
const std::string& BamReader::Filename() const { return d_->filename_; }
const BamHeader& BamReader::Header() const { return d_->header_; }
bool BamReader::GetNext(BamRecord& record)
{
assert(BamRecordMemory::GetRawData(record).get());
const auto result = ReadRawData(d_->handle_.File, BamRecordMemory::GetRawData(record).get());
// success
if (result >= 0) {
BamRecordMemory::UpdateRecordTags(record);
record.header_ = d_->header_;
record.ResetCachedPositions();
#if PBBAM_AUTOVALIDATE
Validator::Validate(record);
#endif
return true;
}
// EOF or end-of-data range (not an error)
else if (result == -1) {
return false;
// error corrupted file
} else {
std::ostringstream msg;
msg << "[pbbam] BAM reader ERROR: cannot read from corrupted file:\n"
<< " file: " << Filename() << '\n'
<< " reason: ";
if (result == -2) {
msg << "probably truncated";
} else if (result == -3) {
msg << "could not read BAM record's' core data";
} else if (result == -4) {
msg << "could not read BAM record's' variable-length data";
} else {
msg << "unknown reason (status code = " << result << ") (" << Filename() << ')';
}
throw std::runtime_error{msg.str()};
}
}
int BamReader::ReadRawData(samFile* file, bam1_t* b) { return bam_read1(file->fp.bgzf, b); }
void BamReader::VirtualSeek(int64_t virtualOffset)
{
const auto result = bgzf_seek(Bgzf(), virtualOffset, SEEK_SET);
if (result != 0) {
std::ostringstream msg;
msg << "[pbbam] BAM reader ERROR: failed to seek:\n"
<< " file: " << Filename() << '\n'
<< " vOffset: " << virtualOffset;
throw std::runtime_error{msg.str()};
}
}
int64_t BamReader::VirtualTell() const { return bgzf_tell(Bgzf()); }
} // namespace BAM
} // namespace PacBio
|