1
2
3
4
5
6
7
8
9
10
11
|
For v1.2
* Use an in vitro PCR server rather than straight BLAST for checking primers. MFEPrimer (http://biocompute.bmi.ac.cn/MFEprimer/) looks a good candidate.
* More intelligent sequencing primer design - search for primers around the midpoint between minimum and maximum distances, and then gradually move out
* More complex primer-dimer routines - including detection of unequal primer-dimer internal loops
* More emphasis on RealTime PCR design? Show primers on intron/exon map, for example?
* Ability to designate regions with starting/ending coordinates or starting/ending sequence strings, and assign colours and PCR ranges to such regions
|
