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ID MYSA_HUMAN STANDARD; PRT; 1939 AA.
AC P13533;
DT 01-JAN-1990 (REL. 13, CREATED)
DT 01-JUN-1994 (REL. 29, LAST SEQUENCE UPDATE)
DT 01-JUN-1994 (REL. 29, LAST ANNOTATION UPDATE)
DE MYOSIN HEAVY CHAIN, CARDIAC MUSCLE ALPHA ISOFORM.
GN MYH6 OR MYHCA.
OS HOMO SAPIENS (HUMAN).
OC EUKARYOTA; METAZOA; CHORDATA; VERTEBRATA; TETRAPODA; MAMMALIA;
OC EUTHERIA; PRIMATES.
RN [1]
RP SEQUENCE FROM N.A.
RX MEDLINE; 92133665.
RA MATSUOKA R., BEISEL K.W., FURUTANI M., ARAI S., TAKAO A.;
RL AM. J. MED. GENET. 41:537-547(1991).
RN [2]
RP SEQUENCE OF 1407-1939 FROM N.A.
RX MEDLINE; 88299163.
RA KURABAYASHI M., TSUCHIMOCHI H., KOMURO I., TAKAKU F., YAZAKI Y.;
RL J. CLIN. INVEST. 82:524-531(1988).
CC -!- FUNCTION: MUSCLE CONTRACTION.
CC -!- SUBUNIT: MUSCLE MYOSIN IS A HEXAMERIC PROTEIN THAT CONSISTS OF 2
CC HEAVY CHAIN SUBUNITS (MHC), 2 ALKALI LIGHT CHAIN SUBUNITS (MLC)
CC AND 2 REGULATORY LIGHT CHAIN SUBUNITS (MLC-2).
CC -!- SUBCELLULAR LOCATION: THICK FILAMENTS OF THE MYOFIBRILS.
CC -!- DOMAIN: THE RODLIKE TAIL SEQUENCE IS HIGHLY REPETITIVE, SHOWING
CC CYCLES OF A 28-RESIDUE REPEAT PATTERN COMPOSED OF 4 HEPTAPEPTIDES,
CC CHARACTERISTIC FOR ALPHA-HELICAL COILED COILS.
CC -!- PTM: TWO CYSTEINE RESIDUES IN THE S1 DOMAIN ARE SELECTIVELY
CC ALKYLATED AND ARE REQUIRED FOR MYOSIN ATPASE ACTIVITY.
CC -!- EACH MYOSIN HEAVY CHAIN CAN BE SPLIT INTO 1 LIGHT MEROMYOSIN (LMM)
CC AND 1 HEAVY MEROMYOSIN (HMM). IT CAN LATTER BE SPLIT FURTHER INTO
CC 2 GLOBULAR SUBFRAGMENTS (S1) AND 1 ROD-SHAPED SUBFRAGMENT (S2).
CC -!- THE CARDIAC ALPHA ISOFORM IS A 'FAST' ATPASE MYOSIN, WHILE THE
CC BETA ISOFORM IS A 'SLOW' ATPASE.
CC -!- SIMILARITY: THE PERIODICITIES OF HYDROPHOBIC AND CHARGED RESIDUES,
CC WHICH DICTATE THE ALPHA-HELICAL COILED-COIL STRUCTURE ARE
CC CONSERVED.
DR EMBL; D00943; G219524; -.
DR EMBL; M21664; G386972; -.
DR PIR; A46762; A46762.
DR PIR; A28908; A28908.
DR HSSP; P24733; 1SCM.
DR MIM; 160710; -.
KW MYOSIN; MUSCLE PROTEIN; COILED COIL; THICK FILAMENT; ACTIN-BINDING;
KW ATP-BINDING; METHYLATION; ALKYLATION; HEPTAD REPEAT PATTERN;
KW MULTIGENE FAMILY.
FT DOMAIN 1 840 GLOBULAR HEAD (S1).
FT DOMAIN 841 1939 RODLIKE TAIL (S2 AND LMM DOMAINS).
FT NP_BIND 178 185 ATP.
FT DOMAIN 657 679 ACTIN-BINDING.
FT DOMAIN 759 773 ACTIN-BINDING.
FT MOD_RES 129 129 METHYLATION (TRI-) (POTENTIAL).
FT MOD_RES 697 697 ALKYLATION (SH-1) (POTENTIAL).
FT MOD_RES 707 707 ALKYLATION (SH-2) (POTENTIAL).
FT CONFLICT 1533 1533 K -> N (IN REF. 2).
FT CONFLICT 1540 1540 L -> M (IN REF. 2).
FT CONFLICT 1577 1578 KL -> NV (IN REF. 2).
FT CONFLICT 1705 1706 DR -> EQ (IN REF. 2).
FT CONFLICT 1763 1763 H -> D (IN REF. 2).
FT CONFLICT 1871 1871 D -> N (IN REF. 2).
FT CONFLICT 1882 1882 R -> G (IN REF. 2).
FT CONFLICT 1890 1890 Q -> R (IN REF. 2).
FT CONFLICT 1933 1933 MISSING (IN REF. 2).
SQ SEQUENCE 1939 AA; 223552 MW; 40812E5A CRC32;
MTDAQMADFG AAAQYLRKSE KERLEAQTRP FDIRTECFVP DDKEEFVKAK ILSREGGKVI
AETENGKTVT VKEDQVLQQN PPKFDKIEDM AMLTFLHEPA VLFNLKERYA AWMIYTYSGL
FCVTVNPYKW LPVYNAEVVA AYRGKKRSEA PPHIFSISDN AYQYMLTDRE NQSILITGES
GAGKTVNTKR VIQYFASIAA IGDRGKKDNA NANKGTLEDQ IIQANPALEA FGNAKTVRND
NSSRFGKFIR IHFGATGKLA SADIETYLLE KSRVIFQLKA ERNYHIFYQI LSNKKPELLD
MLLVTNNPYD YAFVSQGEVS VASIDDSEEL MATDSAFDVL GFTSEEKAGV YKLTGAIMHY
GNMKFKQKQR EEQAEPDGTE DADKSAYLMG LNSADLLKGL CHPRVKVGNE YVTKGQSVQQ
VYYSIGALAK AVYEKMFNWM VTRINATLET KQPRQYFIGV LDIAGFEIFD FNSFEQLCIN
FTNEKLQQFF NHHMFVLEQE EYKKEGIEWT FIDFGMDLQA CIDLIEKPMG IMSILEEECM
FPKATDMTFK AKLYDNHLGK SNNFQKPRNI KGKPEAHFSL IHYAGTVDYN ILGWLEKNKD
PLNETVVGLY QKSSLKLMAT LFSSYATADT GDSGKSKGGK KKGSSFQTVS ALHRENLNKL
MTNLRTTHPH FVRCIIPNER KAPGVMDNPL VMHQLRCNGV LEGIRICRKG FPNRILYGDF
RQRYRILNPV AIPEGQFIDS RKGAEKLLSS LDIDHNQYKF GHTKVFFKAG LLGLLEEMRD
ERLSRIITRI QAQARGQLMR IEFKKIVERR DALLVIQWNI RAFMGVKNWP WMKLYFKIKP
LLKSAETEKE MATMKEEFGR IKETLEKSEA RRKELEEKMV SLLQEKNDLQ LQVQAEQDNL
NDAEERCDQL IKNKIQLEAK VKEMNERLED EEEMNAELTA KKRKLEDECS ELKKDIDDLE
LTLAKVEKEK HATENKVKNL TEEMAGLDEI IAKLTKEKKA LQEAHQQALD DLQAEEDKVN
TLSKSKVKLE QQVDDLEGSL EQEKKVRMDL ERAKRKLEGD LKLTQESIMD LENDKLQLEE
KLKKKEFDIN QQNSKIEDEQ VLALQLQKKL KENQARIEEL EEELEAERTA RAKVEKLRSD
LSRELEEISE RLEEAGGATS VQIEMNKKRE AEFQKMRRDL EEATLQHEAT AAALRKKHAD
SVAELGEQID NLQRVKQKLE KEKSEFKLEL DDVTSNMEQI IKAKANLEKV SRTLEDQANE
YRVKLEEAQR SLNDFTTQRA KLQTENGELS RQLEEKEALI SQLTRGKLSY TQQMEDLKRQ
LEEEGKAKNA LAHALQSARH DCDLLREQYE EETEAKAELQ RVLSKANSEV AQCRTKYETD
AIQRTEELEE AKKKLAQRLQ DAEEAVEAVN AKCSSLEKTK HRLQNEIEDL MVDVERSNAA
AAALDKKQRN FDKILAEWKQ KYEESQSELE SSQKEARSLS TELFKLKNAY EESLEHLETF
KRENKNLQEE ISDLTEQLGE GGKNVHELEK VRKQLEVEKL ELQSALEEAE ASLEHEEGKI
LRAQLEFNQI KAEIERKLAE KDEEMEQAKR NHQRVVDSLQ TSLDAETRSR NEVLRVKKKM
EGDLNEMEIQ LSHANRMAAE AQKQVKSLQS LLKDTQIQLD DAVRANDDLK ENIAIVERRN
NLLQAELEEL RAVVEQTERS RKLADRELIE TSERVQLLHS QNTSLINQKK KMDADLSQLQ
SEVEEAVQEC RNAEEKAKKA ITHAAMMAEE LKKEQDTSAH LERMKKNMEQ TIKDLQHRLD
EAEQIALKGG KKQLQKLEAR VRELEGELEA EQKRNAESVK GMRKSERRIK ELTYQTEEDK
KNLLRLQDLV DKLQLKVKAY KRQAEEAEEQ ANTNLSKFRK VQHELDEAEE RADIAESQVN
KLRAKSRDIG AKQKMHDEE
//
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