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#include "piler2.h"
#define GFFRecord GFFRecord2
// GFF fields are:
// <seqname> <source> <feature> <start> <end> <score> <strand> <frame> [attributes] [comments]
// 0 1 2 3 4 5 6 7 8 9
static int GFFLineNr2;
bool HasTargetAttrs(const char *Attrs)
{
const char *ptrTarget = strstr(Attrs, "Target ");
return 0 != ptrTarget;
}
// Piles 123 456
// 0123456
void ParsePilesAttrs(const char *Attrs, int *ptrQueryPile, int *ptrTargetPile)
{
const char *ptrPiles = strstr(Attrs, "Piles ");
if (0 == ptrPiles)
Quit("Piles attribute not found");
const char *ptrValues = ptrPiles + 6;
int n = sscanf(ptrValues, "%d %d", ptrQueryPile, ptrTargetPile);
if (n != 2)
Quit("ParsePilesAttrs, sscanf(%s)=%d", ptrValues, n);
}
// BandClust 123
// 01234567890
void ParseBandClustAttrs(const char *Attrs, int *ptrBandClustIndex)
{
const char *ptrBandClust = strstr(Attrs, "BandClust ");
if (0 == ptrBandClust)
Quit("BandClust attribute not found");
const char *ptrIndex = ptrBandClust + 10;
*ptrBandClustIndex = atoi(ptrIndex);
}
// Pyramid 123
// 01234567890
void ParsePyramidAttrs(const char *Attrs, int *ptrClustIndex)
{
const char *p = strstr(Attrs, "Pyramid ");
if (0 == p)
Quit("Pyramid attribute not found");
const char *ptrIndex = p + 8;
*ptrClustIndex = atoi(ptrIndex);
}
// Target SeqName 123 456
// 01234567
void ParseTargetAttrs(const char *Attrs, char SeqName[],
int SeqNameBytes, int *ptrStart, int *ptrEnd)
{
const char *ptrTarget = strstr(Attrs, "Target ");
if (0 == ptrTarget)
Quit("Target attribute not found");
const char *ptrRest = ptrTarget + 7;
const char *ptrBlank = strchr(ptrRest, ' ');
if (0 == ptrBlank)
Quit("Invalid Target attributes '%s', missing blank", Attrs);
size_t NameLength = ptrBlank - ptrRest;
if (NameLength >= (size_t) SeqNameBytes)
Quit("Target name length too long '%s'", Attrs);
memcpy(SeqName, ptrRest, NameLength);
SeqName[NameLength] = 0;
int n = sscanf(ptrBlank+1, "%d %d", ptrStart, ptrEnd);
if (n != 2)
Quit("Invalid Target start/end attributes '%s', sscanf=%d", ptrBlank+1);
}
// Destructive read -- pokes nuls onto FS
static int GetFields2(char *Line, char **Fields, int MaxFields, char FS)
{
char *p = Line;
for (int FieldIndex = 0; FieldIndex < MaxFields; ++FieldIndex)
{
Fields[FieldIndex] = p;
char *Tab = strchr(p, FS);
char *End = Tab;
if (0 == End)
End = strchr(p, '\0');
size_t FieldLength = End - p;
if (FieldLength > MAX_GFF_FEATURE_LENGTH)
Quit("Max GFF field length exceeded, field is %d chars, max=%d, line %d",
FieldLength, MAX_GFF_FEATURE_LENGTH, GFFLineNr2);
if (0 == Tab)
return FieldIndex + 1;
*Tab = 0;
p = Tab + 1;
}
return MaxFields;
}
// WARNING: Line[] and Fields[]are overwritten
// by each call to GetNextGFFRecord.
// The Rec agument to GetNextGFFRecord returns
// pointers into Line[].
static char Line[MAX_GFF_LINE+1];
static char *Fields[9];
bool GetNextGFFRecord(FILE *f, GFFRecord &Rec)
{
for (;;)
{
++GFFLineNr2;
const char TAB = '\t';
char *Ok = fgets(Line, sizeof(Line), f);
if (NULL == Ok)
{
if (feof(f))
return false;
Quit("Error reading GFF file, line=%d feof=%d ftell=%d ferror=%d errno=%d",
GFFLineNr2, feof(f), ftell(f), ferror(f), errno);
}
if ('#' == Line[0])
continue;
size_t n = strlen(Line);
if (0 == n)
Quit("fgets returned zero-length line");
if (Line[n-1] != '\n')
Quit("Max line length in GFF file exceeded, line %d is %d chars long, max=%d",
GFFLineNr2, n - 1, MAX_GFF_LINE);
Line[n-1] = 0; // delete newline
int FieldCount = GetFields2(Line, Fields, 9, '\t');
if (FieldCount < 8)
Quit("GFF record has < 8 fields, line %d", GFFLineNr2);
char *SeqName = Fields[0];
char *Source = Fields[1];
char *Feature = Fields[2];
char *Start = Fields[3];
char *End = Fields[4];
char *Score = Fields[5];
char *Strand = Fields[6];
char *Frame = Fields[7];
char *Attrs = Fields[8];
// Truncate attrs if comment found
char *Pound = strchr(Attrs, '#');
if (0 != Pound)
*Pound = 0;
Rec.SeqName = SeqName;
Rec.Source = Source;
Rec.Feature = Feature;
Rec.Start = atoi(Start);
Rec.End = atoi(End);
Rec.Score = (float) atof(Score);
Rec.Strand = Strand[0];
Rec.Frame = Frame[0] == '.' ? -1 : atoi(Frame);
Rec.Attrs = Attrs;
return true;
}
}
// GFF fields are:
// <seqname> <source> <feature> <start> <end> <score> <strand> <frame> [attributes] [comments]
// 0 1 2 3 4 5 6 7 8 9
void WriteGFFRecord(FILE *f, const GFFRecord &Rec)
{
fprintf(f, "%s\t%s\t%s\t%d\t%d\t%.3g\t%c",
// 0 1 2 3 4 5 6 7 8
Rec.SeqName, // 0
Rec.Source, // 1
Rec.Feature, // 2
Rec.Start, // 3
Rec.End, // 4
Rec.Score, // 5
Rec.Strand); // 6
if (-1 == Rec.Frame)
fprintf(f, "\t.");
else
fprintf(f, "\t%d", Rec.Frame);
fprintf(f, "\t%s\n", Rec.Attrs);
}
void SaveGFFStrings(GFFRecord &Rec)
{
Rec.SeqName = strsave(Rec.SeqName);
Rec.Feature = strsave(Rec.Feature);
Rec.Source = strsave(Rec.Source);
Rec.Attrs = strsave(Rec.Attrs);
}
void FreeGFFStrings(GFFRecord &Rec)
{
free((void *) Rec.SeqName);
free((void *) Rec.Feature);
free((void *) Rec.Source);
free((void *) Rec.Attrs);
Rec.SeqName = 0;
Rec.Feature = 0;
Rec.Source = 0;
Rec.Attrs = 0;
}
void GFFRecordToHit(const GLIX &Glix, const GFFRecord &Rec, HitData &Hit)
{
if (0 != strcmp(Rec.Feature, "hit"))
Quit("GFFRecordToHit: feature=%s", Rec.Feature);
const int QueryLength = Rec.End - Rec.Start + 1;
Hit.QueryFrom = Glix.LocalToGlobal(Rec.SeqName, Rec.Start - 1);
Hit.QueryTo = Hit.QueryFrom + QueryLength - 1;
char TargetName[MAX_GFF_FEATURE_LENGTH+1];
int TargetStart;
int TargetEnd;
ParseTargetAttrs(Rec.Attrs, TargetName, sizeof(TargetName), &TargetStart, &TargetEnd);
const int TargetLength = TargetEnd - TargetStart + 1;
Hit.TargetFrom = Glix.LocalToGlobal(TargetName, TargetStart - 1);
Hit.TargetTo = Hit.TargetFrom + TargetLength - 1;
Hit.Rev = (Rec.Strand == '-');
}
void HitToGFFRecord(const GLIX &Glix, const HitData &Hit, GFFRecord &Rec, char AnnotBuffer[])
{
Rec.Source = "piler";
Rec.Feature = "hit";
Rec.Score = 0;
Rec.Frame = '.';
Rec.Strand = (Hit.Rev ? '-' : '+');
const int QueryLength = Hit.QueryTo - Hit.QueryFrom + 1;
int SeqQueryFrom;
const char *QueryLabel = Glix.GlobalToSeqPadded(Hit.QueryFrom, &SeqQueryFrom);
const int SeqQueryTo = SeqQueryFrom + QueryLength - 1;
Rec.SeqName = QueryLabel;
Rec.Start = SeqQueryFrom + 1;
Rec.End = SeqQueryTo + 1;
const int TargetLength = Hit.TargetTo - Hit.TargetFrom + 1;
int SeqTargetFrom;
const char *TargetLabel = Glix.GlobalToSeqPadded(Hit.TargetFrom, &SeqTargetFrom);
const int SeqTargetTo = SeqTargetFrom + TargetLength - 1;
sprintf(AnnotBuffer, "Target %s %d %d", TargetLabel, SeqTargetFrom + 1, SeqTargetTo + 1);
Rec.Attrs = AnnotBuffer;
}
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