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#include "pilercr.h"
#include "sw.h"
int UnalignedLength(const std::string &s)
{
size_t L = s.size();
int N = 0;
for (size_t i = 0; i < L; ++i)
if (s[i] != '-')
++N;
return N;
}
static bool GloballyAlignable(const char *Seq1, unsigned L1, const char *Seq2,
unsigned L2)
{
std::string Path;
unsigned Start1;
unsigned Start2;
score_t Score = SWSimple(Seq1, L1, Seq2, L2, &Start1, &Start2, Path);
if (Score <= 0)
return false;
int AlnLen = (int) Path.size();
if (GetRatio(AlnLen, (int) L1) < 0.95)
return false;
return true;
}
// is i = nj for n approximately integer in range 1 .. 4?
static int Multiple(int i, int j)
{
if (GetRatio(i, 1*j) >= 0.95)
return 1;
if (GetRatio(i, 2*j) >= 0.95)
return 2;
if (GetRatio(i, 3*j) >= 0.95)
return 3;
if (GetRatio(i, 4*j) >= 0.95)
return 4;
return -1;
}
int DistanceAA(const ArrayAln &AA1, const ArrayAln &AA2)
{
int End1 = AA1.Pos + GetArrayLength(AA1) + GetAvgSpacerLength(AA1) - 1;
int Pos2 = AA2.Pos;
int ContigIndex1 = GlobalPosToContigIndex(End1);
int ContigIndex2 = GlobalPosToContigIndex(Pos2);
if (ContigIndex1 != ContigIndex2)
return -1;
int ci1 = GlobalPosToContigIndex(AA1.Pos);
if (ContigIndex1 != ci1)
Warning("ContigIndex1=%u GlobalPosToContigIndex(AA1.Pos=%d) = %d",
ContigIndex1, AA1.Pos, ci1);
return Pos2 - End1 - 1;
}
bool MergeAAs(ArrayAln &AA1, const ArrayAln &AA2)
{
int RepeatLength1 = GetAvgRepeatLength(AA1);
int RepeatLength2 = GetAvgRepeatLength(AA2);
if (RepeatLength1 != RepeatLength2)
return false;
int SpacerLength1 = GetAvgSpacerLength(AA1);
int SpacerLength2 = GetAvgSpacerLength(AA2);
if (GetRatio(SpacerLength1, SpacerLength2) < 0.95)
return false;
// Is distance between arrays a multiple of repeat+spacer length?
int Distance = DistanceAA(AA1, AA2);
int RepeatPlusSpacerLength = RepeatLength1 + SpacerLength1;
int MissedRepeatCount = Multiple(Distance, RepeatPlusSpacerLength);
if (MissedRepeatCount < 0)
return false;
int ConsSeqLength1 = AA1.ConsSeq.Length();
int ConsSeqLength2 = AA2.ConsSeq.Length();
char *Seq1 = all(char, ConsSeqLength1 + 1);
char *Seq2 = all(char, ConsSeqLength2 + 1);
AA1.ConsSeq.ToString(Seq1, ConsSeqLength1 + 1);
AA2.ConsSeq.ToString(Seq2, ConsSeqLength2 + 1);
if (!GloballyAlignable(Seq1, ConsSeqLength1, Seq2, ConsSeqLength2))
return false;
IntVec RepeatStartPos;
IntVec RepeatLengths;
int LastSpacerStartPos = AA1.Pos + GetArrayLength(AA1);
int EndPosOfLastRepeat = LastSpacerStartPos - 1;
StrVec Repeats;
for (int i = 0; i < MissedRepeatCount; ++i)
{
int GuessedRepeatPos = EndPosOfLastRepeat + SpacerLength1;
int StartPos = GuessedRepeatPos - 8;
int EndPos = GuessedRepeatPos + RepeatLength1 + 8;
int SeqLength = EndPos - StartPos + 1;
char *Seq = all(char, SeqLength);
for (int i = 0; i < SeqLength; ++i)
Seq[i] = g_SeqQ[StartPos + i];
char *ConsSeq = all(char, ConsSeqLength1 + 1);
AA1.ConsSeq.ToString(ConsSeq, ConsSeqLength1 + 1);
std::string Path;
unsigned StartSeq;
unsigned StartCons;
score_t Score = SWSimple(Seq, SeqLength, ConsSeq, ConsSeqLength1,
&StartSeq, &StartCons, Path);
int AlnLength = (int) Path.size();
if (GetRatio(AlnLength, ConsSeqLength1) < 0.95)
return false;
std::string &Repeat = *new std::string;
for (int i = 0; i < (int) StartCons; ++i)
Repeat.push_back('-');
int SeqPos = StartPos + StartSeq;
int RepeatPos = SeqPos;
RepeatStartPos.push_back(RepeatPos);
int RepeatLength = 0;
for (int i = 0; i < AlnLength; ++i)
{
if (Path[i] == 'M' || Path[i] == 'D')
{
char c = g_SeqQ[SeqPos];
Repeat.push_back(c);
++SeqPos;
++RepeatLength;
}
else
Repeat.push_back('-');
}
if (!RepeatLengths.empty() && RepeatLength != RepeatLengths[0])
return false;
RepeatLengths.push_back(RepeatLength);
EndPosOfLastRepeat = RepeatPos + RepeatLength - 1;
while (Repeat.size() < (size_t) ConsSeqLength1)
Repeat.push_back('-');
Repeats.push_back(Repeat);
}
for (int i = 0; i < MissedRepeatCount; ++i)
AA1.Repeats.push_back(Repeats[i]);
for (int i = 0; i < MissedRepeatCount; ++i)
{
std::string &LeftFlank = *new std::string;
int LeftFlankStartPos = RepeatStartPos[i] - g_FlankSize;
for (int i = 0; i < g_FlankSize; ++i)
{
char c = g_SeqQ[LeftFlankStartPos + i];
LeftFlank.push_back(c);
}
AA1.LeftFlanks.push_back(LeftFlank);
}
int OldCount = (int) AA1.Spacers.size();
std::string &Spacer = *new std::string;
int RepeatStartPos0 = RepeatStartPos[0];
int LastSpacerEndPos = RepeatStartPos0 - 1;
assert(LastSpacerEndPos > LastSpacerStartPos);
int Length = LastSpacerEndPos - LastSpacerStartPos + 1;
for (int i = 0; i < Length; ++i)
{
char c = g_SeqQ[LastSpacerStartPos + i];
Spacer.push_back(c);
}
AA1.Spacers[OldCount-1] = Spacer;
for (int i = 0; i < MissedRepeatCount; ++i)
{
std::string &Spacer = *new std::string;
int SpacerStartPos = RepeatStartPos[i] + UnalignedLength(AA1.Repeats[OldCount+i]);
int SpacerEndPos;
if (i == MissedRepeatCount - 1)
SpacerEndPos = AA2.Pos - 1;
else
SpacerEndPos = RepeatStartPos[i+1] - 1;
assert(SpacerEndPos > SpacerStartPos);
int Length = SpacerEndPos - SpacerStartPos + 1;
for (int i = 0; i < Length; ++i)
{
char c = g_SeqQ[SpacerStartPos + i];
Spacer.push_back(c);
}
AA1.Spacers.push_back(Spacer);
}
size_t RepeatCount2 = AA2.Repeats.size();
size_t RepeatLength = 0;
for (size_t RepeatIndex = 0; RepeatIndex < RepeatCount2; ++RepeatIndex)
{
AA1.LeftFlanks.push_back(AA2.LeftFlanks[RepeatIndex]);
std::string Repeat = AA2.Repeats[RepeatIndex];
AA1.Repeats.push_back(Repeat);
const std::string &Spacer = AA2.Spacers[RepeatIndex];
size_t L = Spacer.size();
AA1.Spacers.push_back(Spacer);
}
ValidateAA(AA1);
Log("MergeAAs\n");
return true;
}
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