File: primer_check_input

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primer3 2.6.1-6
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file content (113 lines) | stat: -rw-r--r-- 4,193 bytes parent folder | download | duplicates (8) 
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SEQUENCE_ID=00
PRIMER_MAX_POLY_X=0
PRIMER_INTERNAL_MAX_POLY_X=0
PRIMER_EXPLAIN_FLAG=1
PRIMER_PRODUCT_SIZE_RANGE=50-100
PRIMER_MIN_TM=0.0
PRIMER_MAX_TM=100.0
PRIMER_MIN_SIZE=25
PRIMER_MAX_SIZE=25
PRIMER_INTERNAL_MIN_SIZE=25
PRIMER_INTERNAL_MAX_SIZE=25
PRIMER_INTERNAL_OPT_SIZE=25
PRIMER_OPT_SIZE=25
PRIMER_MAX_GC=100
PRIMER_MIN_GC=0
PRIMER_MAX_SELF_END=100
PRIMER_MAX_SELF_ANY=100
PRIMER_PAIR_MAX_COMPL_ANY=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_INTERNAL_MAX_SELF_ANY=100
PRIMER_INTERNAL_MAX_SELF_END=100 
PRIMER_INTERNAL_MAX_TM=100
PRIMER_INTERNAL_MIN_TM=30
PRIMER_INTERNAL_MAX_GC=100
PRIMER_INTERNAL_MIN_GC=0
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=01
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=02
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
PRIMER_PICK_INTERNAL_OLIGO=0
=
SEQUENCE_ID=03
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=04
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=05
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=06
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=07
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
PRIMER_PICK_INTERNAL_OLIGO=0
=
SEQUENCE_ID=08
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
PRIMER_PICK_INTERNAL_OLIGO=0
=
SEQUENCE_ID=09
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=10
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=11
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=GAATTGTTTGGAAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=12
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATCTCATCAAATTTGATTG
SEQUENCE_PRIMER_REVCOMP=TGCGTCGACTGCAACGTACGTACGT
SEQUENCE_INTERNAL_OLIGO=ACGTACGTACGTTGCAGTCGACGCA
PRIMER_PICK_INTERNAL_OLIGO=0
=
SEQUENCE_ID=13
SEQUENCE_TEMPLATE=nncacgtcaTCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=cacgtcatctcatcaaatttgattg
SEQUENCE_PRIMER_REVCOMP=gaattgtttggaaatgacagtgagt
PRIMER_PICK_INTERNAL_OLIGO=1
=
SEQUENCE_ID=14
SEQUENCE_TEMPLATE=NNCACGTCATCTCATCAAATTTGATTGNNNACGTACGTACGTTGCAGTCGACGCANNACTCACTGTCATTTCCAAACAATTCNN
SEQUENCE_PRIMER=CACGTCATctcatcaaattTGATTG
SEQUENCE_PRIMER_REVCOMP=gaattgtttggaAATGACAGTGAGT
PRIMER_PICK_INTERNAL_OLIGO=1
=