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SEQUENCE_ID=syntax01
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
P3_COMMENT="should succeeed"
PRIMER_LEFT_EXPLAIN=considered 25, too many Ns 10, low tm 15, ok 0
PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=syntax02
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
P3_COMMENT="should succeeed"
PRIMER_LEFT_EXPLAIN=considered 25, too many Ns 10, low tm 15, ok 0
PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=syntax03
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
P3_COMMENT="should succeeed"
PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=syntax04
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
P3_COMMENT="should succeeed"
PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=syntax05
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5 2,
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
P3_COMMENT="should cause error"
PRIMER_ERROR=Illegal SEQUENCE_TARGET value: 2,
=
SEQUENCE_ID=syntax06
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5 2
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
P3_COMMENT="should cause error"
PRIMER_ERROR=Illegal SEQUENCE_TARGET value: 2
=
SEQUENCE_ID=syntax07
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21 0,1
P3_COMMENT="should succeed"
PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=syntax08
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21,1
P3_COMMENT="should cause error"
PRIMER_ERROR=Illegal SEQUENCE_INTERNAL_EXCLUDED_REGION value: ,1
=
SEQUENCE_ID=syntax09
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=
P3_COMMENT="should succeed"
PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=syntax10
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21 3,5
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=
P3_COMMENT="should succeed (whitespace in int. oligo. excl. region)"
PRIMER_LEFT_EXPLAIN=considered 15, too many Ns 10, overlap target 5, ok 0
PRIMER_RIGHT_EXPLAIN=considered 63, low tm 51, high tm 1, high any compl 4, ok 7
PRIMER_INTERNAL_EXPLAIN=considered 0, ok 0
PRIMER_PAIR_EXPLAIN=considered 0, ok 0
PRIMER_LEFT_NUM_RETURNED=0
PRIMER_RIGHT_NUM_RETURNED=0
PRIMER_INTERNAL_NUM_RETURNED=0
PRIMER_PAIR_NUM_RETURNED=0
=
SEQUENCE_ID=syntax11
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21.0
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37,21
PRIMER_EXPLAIN_FLAG=1
P3_COMMENT=should fail with syntax error
PRIMER_ERROR=Illegal SEQUENCE_TARGET value: .0
=
SEQUENCE_ID=syntax12
SEQUENCE_TEMPLATE=GTAGTCAGTAGACNATGACNACTGACGATGCAGACNACACACACACACACAGCACACAGGTATTAGTGGGCCATTCGATCCCGACCCAAATCGATAGCTACGATGACG
SEQUENCE_TARGET=37,21
PRIMER_OPT_SIZE=18
PRIMER_MIN_SIZE=15
PRIMER_MAX_SIZE=21
PRIMER_MAX_NS_ACCEPTED=1
P3_FILE_FLAG=1
PRIMER_PICK_INTERNAL_OLIGO=1
SEQUENCE_INTERNAL_EXCLUDED_REGION=37.01,21
PRIMER_EXPLAIN_FLAG=1
P3_COMMENT=should fail with type error.
PRIMER_ERROR=Illegal SEQUENCE_INTERNAL_EXCLUDED_REGION value: 37.01,21
=
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