1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398
|
#!/usr/bin/env python
# -*- coding: utf-8 -*-
# -----------------------------------------------------------------------------
# Copyright (c) 2011-2017, The BIOM Format Development Team.
#
# Distributed under the terms of the Modified BSD License.
#
# The full license is in the file COPYING.txt, distributed with this software.
# -----------------------------------------------------------------------------
from os import remove
from os.path import abspath, dirname, exists
from tempfile import NamedTemporaryFile
from unittest import TestCase, main
import numpy as np
import numpy.testing as npt
from biom.table import Table
from biom.parse import parse_biom_table, load_table
from biom.util import (natsort, flatten, unzip, HAVE_H5PY,
get_biom_project_dir, parse_biom_config_files,
compute_counts_per_sample_stats, safe_md5, biom_open,
get_data_path, generate_subsamples, is_hdf5_file)
np.random.seed(1234)
__author__ = "Daniel McDonald"
__copyright__ = "Copyright 2011-2017, The BIOM Format Development Team"
__credits__ = ["Rob Knight", "Peter Maxwell", "Sandra Smit",
"Zongzhi Liu", "Micah Hamady", "Daniel McDonald",
"Jai Ram Rideout", "Jorge Cañardo Alastuey"]
__license__ = "BSD"
__url__ = "http://biom-format.org"
__maintainer__ = "Daniel McDonald"
__email__ = "daniel.mcdonald@colorado.edu"
if HAVE_H5PY:
import h5py
class UtilTests(TestCase):
def setUp(self):
self.biom_otu_table1_w_tax = parse_biom_table(biom_otu_table1_w_tax)
def test_generate_subsamples(self):
table = Table(np.array([[3, 1, 1], [0, 3, 3]]), ['O1', 'O2'],
['S1', 'S2', 'S3'])
actual_o1 = set()
actual_o2 = set()
for i, obs in zip(range(100), generate_subsamples(table, 3)):
actual_o1.add(tuple(obs.data('O1', 'observation')))
actual_o2.add(tuple(obs.data('O2', 'observation')))
self.assertEqual(actual_o1, {(3, 0, 0), (3, 1, 0), (3, 0, 1),
(3, 1, 1)})
self.assertEqual(actual_o2, {(0, 3, 3), (0, 2, 3), (0, 3, 2),
(0, 2, 2)})
def test_natsort(self):
"""natsort should perform numeric comparisons on strings
This method is ported from QIIME (http://www.qiime.org). QIIME is a GPL
project, but we obtained permission from the authors of this method to
port it to the BIOM Format project (and keep it under BIOM's BSD
license).
"""
# string with alpha and numerics sort correctly
s = 'sample1 sample2 sample11 sample12'.split()
self.assertEqual(natsort(s),
'sample1 sample2 sample11 sample12'.split())
s.reverse()
self.assertEqual(natsort(s),
'sample1 sample2 sample11 sample12'.split())
self.assertEqual(natsort(list('cba321')), list('123abc'))
# strings with alpha only sort correctly
self.assertEqual(natsort(list('cdba')), list('abcd'))
# string of ints sort correctly
self.assertEqual(natsort(['11', '2', '1', '0']),
['0', '1', '2', '11'])
# strings of floats sort correctly
self.assertEqual(natsort(['1.11', '1.12', '1.00', '0.009']),
['0.009', '1.00', '1.11', '1.12'])
# string of ints sort correctly
self.assertEqual(
natsort([('11', 'A'), ('2', 'B'), ('1', 'C'), ('0', 'D')]),
[('0', 'D'), ('1', 'C'), ('2', 'B'), ('11', 'A')])
def test_unzip(self):
"""unzip(items) should be the inverse of zip(*items)
This method is ported from PyCogent (http://www.pycogent.org). PyCogent
is a GPL project, but we obtained permission from the authors of this
method to port it to the BIOM Format project (and keep it under BIOM's
BSD license).
"""
chars = [list('abcde'), list('ghijk')]
numbers = [[1, 2, 3, 4, 5], [0, 0, 0, 0, 0]]
strings = [["abcde", "fghij", "klmno"], ['xxxxx'] * 3]
lists = [chars, numbers, strings]
zipped = [zip(*i) for i in lists]
unzipped = [unzip(i) for i in zipped]
for u, l in zip(unzipped, lists):
self.assertEqual(u, l)
def test_flatten_no_change(self):
"""flatten should not change non-nested sequences (except to list)
This method is ported from PyCogent (http://www.pycogent.org). PyCogent
is a GPL project, but we obtained permission from the authors of this
method to port it to the BIOM Format project (and keep it under BIOM's
BSD license).
"""
self.assertEqual(flatten('abcdef'), list('abcdef')) # test identities
self.assertEqual(flatten([]), []) # test empty sequence
self.assertEqual(flatten(''), []) # test empty string
def test_flatten(self):
"""flatten should remove one level of nesting from nested sequences
This method is ported from PyCogent (http://www.pycogent.org). PyCogent
is a GPL project, but we obtained permission from the authors of this
method to port it to the BIOM Format project (and keep it under BIOM's
BSD license).
"""
self.assertEqual(flatten(['aa', 'bb', 'cc']), list('aabbcc'))
self.assertEqual(flatten([1, [2, 3], [[4, [5]]]]), [1, 2, 3, [4, [5]]])
def test_get_biom_project_dir(self):
"""Getting the biom project directory functions as expected.
This method is ported from QIIME (http://www.qiime.org). QIIME is a GPL
project, but we obtained permission from the authors of this method to
port it to the BIOM Format project (and keep it under BIOM's BSD
license).
"""
# Do an explicit check on whether the file system containing
# the current file is case insensitive. This is in response
# to SF bug #2945548, where this test would fail on certain
# unusual circumstances on case-insensitive file systems
# because the case of abspath(__file__) was inconsistent.
# (If you don't believe this, set case_insensitive_filesystem
# to False, and rename your top-level biom-format directory as
# Biom-format on OS X. That should cause this test to fail as
# actual will be path/to/Biom-format and expected will be
# path/to/biom-format.) Note that we don't need to change anything
# in the get_biom_project_dir() function as if the
# file system is case insenstive, the case of the returned
# string is irrelevant.
case_insensitive_filesystem = \
exists(__file__.upper()) and exists(__file__.lower())
actual = get_biom_project_dir()
# I base the expected here off the imported location of
# biom/util.py here, to handle cases where either the user
# has biom-format in their PYTHONPATH, or when they've installed it
# with setup.py.
# If util.py moves this test will fail -- that
# is what we want in this case, as the get_biom_project_dir()
# function would need to be modified.
import biom.util
util_py_filepath = abspath(abspath(biom.util.__file__))
expected = dirname(dirname(dirname(util_py_filepath)))
if case_insensitive_filesystem:
# Make both lowercase if the file system is case insensitive.
actual = actual.lower()
expected = expected.lower()
self.assertEqual(actual, expected)
def test_parse_biom_config_files(self):
"""parse_biom_config_files functions as expected.
This method is ported from QIIME (http://www.qiime.org). QIIME is a GPL
project, but we obtained permission from the authors of this method to
port it to the BIOM Format project (and keep it under BIOM's BSD
license).
"""
fake_file1 = ['key1\tval1', 'key2 val2']
fake_file2 = ['key2\tval3']
actual = parse_biom_config_files([fake_file1, fake_file2])
expected = {'key1': 'val1', 'key2': 'val3'}
self.assertEqual(actual, expected)
# Looking up a nonexistent value returns None.
self.assertEqual(actual['fake_key'], None)
# Empty dict on empty input.
self.assertEqual(parse_biom_config_files([]), {})
def test_compute_counts_per_sample_stats_empty(self):
t = Table({}, [], [])
res = compute_counts_per_sample_stats(t)
self.assertEqual(res, (0, 0, 0, 0, {}))
def test_compute_counts_per_sample_stats(self):
"""compute_counts_per_sample_stats functions as expected
This method is ported from QIIME (http://www.qiime.org). QIIME is a GPL
project, but we obtained permission from the authors of this method to
port it to the BIOM Format project (and keep it under BIOM's BSD
license).
"""
actual = compute_counts_per_sample_stats(self.biom_otu_table1_w_tax)
self.assertEqual(actual[0], 3)
self.assertEqual(actual[1], 7)
self.assertEqual(actual[2], 4)
self.assertEqual(actual[3], 4.5)
self.assertEqual(actual[4], {'Sample1': 7, 'Sample2': 3, 'Sample3': 4,
'Sample4': 6, 'Sample5': 3, 'Sample6': 4})
def test_compute_counts_per_sample_stats_obs_counts(self):
"""compute_counts_per_sample_stats functions as expected
This method is ported from QIIME (http://www.qiime.org). QIIME is a GPL
project, but we obtained permission from the authors of this method to
port it to the BIOM Format project (and keep it under BIOM's BSD
license).
"""
actual = compute_counts_per_sample_stats(self.biom_otu_table1_w_tax,
binary_counts=True)
self.assertEqual(actual[0], 1)
self.assertEqual(actual[1], 4)
self.assertEqual(actual[2], 2.5)
self.assertEqual(actual[3], 2.5)
self.assertEqual(actual[4], {'Sample1': 2, 'Sample2': 3, 'Sample3': 4,
'Sample4': 2, 'Sample5': 1, 'Sample6': 3})
def test_safe_md5(self):
"""Make sure we have the expected md5 with varied input types
This method is ported from PyCogent (http://www.pycogent.org). PyCogent
is a GPL project, but we obtained permission from the authors of this
method to port it to the BIOM Format project (and keep it under BIOM's
BSD license).
"""
exp = 'd3b07384d113edec49eaa6238ad5ff00'
tmp_f = NamedTemporaryFile(
mode='w',
prefix='test_safe_md5',
suffix='txt')
tmp_f.write('foo\n')
tmp_f.flush()
obs = safe_md5(open(tmp_f.name, 'r'))
self.assertEqual(obs, exp)
obs = safe_md5(['foo\n'])
self.assertEqual(obs, exp)
# unsupported type raises TypeError
self.assertRaises(TypeError, safe_md5, 42)
@npt.dec.skipif(HAVE_H5PY is False, msg='H5PY is not installed')
def test_biom_open_hdf5(self):
with biom_open(get_data_path('test.biom')) as f:
self.assertTrue(isinstance(f, h5py.File))
with biom_open(get_data_path('test_writing.biom'), 'w') as f:
self.assertTrue(isinstance(f, h5py.File))
remove(get_data_path('test_writing.biom'))
def test_biom_open_empty(self):
with self.assertRaises(ValueError) as e:
with biom_open(get_data_path('no-contents.biom'), 'r'):
pass
self.assertTrue("is empty and can't be parsed" in str(e.exception))
@npt.dec.skipif(HAVE_H5PY, msg='Can only be tested without H5PY')
def test_biom_open_hdf5_no_h5py(self):
with self.assertRaises(RuntimeError):
with biom_open(get_data_path('test.biom')):
pass
def test_biom_open_json(self):
with biom_open(get_data_path('test.json')) as f:
self.assertTrue(hasattr(f, 'read'))
def test_load_table_gzip_unicode(self):
t = load_table(get_data_path('bad_table.txt.gz'))
self.assertEqual(u's__Cortinarius grosmornënsis',
t.metadata('otu1', 'observation')['taxonomy'])
def test_load_table_unicode(self):
t = load_table(get_data_path('bad_table.txt'))
self.assertEqual(u's__Cortinarius grosmornënsis',
t.metadata('otu1', 'observation')['taxonomy'])
def test_is_hdf5_file(self):
self.assertTrue(is_hdf5_file(get_data_path('test.biom')))
self.assertFalse(is_hdf5_file(get_data_path('test.json')))
def test_load_classic(self):
tab = load_table(get_data_path('test.json'))
with NamedTemporaryFile(mode='w') as fp:
fp.write(str(tab))
fp.flush()
obs = load_table(fp.name)
npt.assert_equal(obs.ids(), tab.ids())
npt.assert_equal(obs.ids(axis='observation'),
tab.ids(axis='observation'))
npt.assert_equal(obs.matrix_data.toarray(), tab.matrix_data.toarray())
biom_otu_table1_w_tax = """{
"id":null,
"format": "Biological Observation Matrix 1.0.0-dev",
"format_url": "http://biom-format.org",
"type": "OTU table",
"generated_by": "QIIME revision XYZ",
"date": "2011-12-19T19:00:00",
"rows":[
{"id":"GG_OTU_1", "metadata":{"taxonomy":["k__Bacteria", "p__Proteoba\
cteria", "c__Gammaproteobacteria", "o__Enterobacteriales", "f__Enterobacteriac\
eae", "g__Escherichia", "s__"]}},
{"id":"GG_OTU_2", "metadata":{"taxonomy":["k__Bacteria", "p__Cyanobact\
eria", "c__Nostocophycideae", "o__Nostocales", "f__Nostocaceae", "g__Dolichosp\
ermum", "s__"]}},
{"id":"GG_OTU_3", "metadata":{"taxonomy":["k__Archaea", "p__Euryarchae\
ota", "c__Methanomicrobia", "o__Methanosarcinales", "f__Methanosarcinaceae", "\
g__Methanosarcina", "s__"]}},
{"id":"GG_OTU_4", "metadata":{"taxonomy":["k__Bacteria", "p__Firmicute\
s", "c__Clostridia", "o__Halanaerobiales", "f__Halanaerobiaceae", "g__Halanaer\
obium", "s__Halanaerobiumsaccharolyticum"]}},
{"id":"GG_OTU_5", "metadata":{"taxonomy":["k__Bacteria", "p__Proteobac\
teria", "c__Gammaproteobacteria", "o__Enterobacteriales", "f__Enterobacteriace\
ae", "g__Escherichia", "s__"]}}
],
"columns":[
{"id":"Sample1", "metadata":{
"BarcodeSequence":"CGCTTATCGAGA",
"LinkerPrimerSequence":"CATGCTGCCTCCCGTAGGAGT",
"BODY_SITE":"gut",
"Description":"human gut"}},
{"id":"Sample2", "metadata":{
"BarcodeSequence":"CATACCAGTAGC",
"LinkerPrimerSequence":"CATGCTGCCTCCCGTAGGAGT",
"BODY_SITE":"gut",
"Description":"human gut"}},
{"id":"Sample3", "metadata":{
"BarcodeSequence":"CTCTCTACCTGT",
"LinkerPrimerSequence":"CATGCTGCCTCCCGTAGGAGT",
"BODY_SITE":"gut",
"Description":"human gut"}},
{"id":"Sample4", "metadata":{
"BarcodeSequence":"CTCTCGGCCTGT",
"LinkerPrimerSequence":"CATGCTGCCTCCCGTAGGAGT",
"BODY_SITE":"skin",
"Description":"human skin"}},
{"id":"Sample5", "metadata":{
"BarcodeSequence":"CTCTCTACCAAT",
"LinkerPrimerSequence":"CATGCTGCCTCCCGTAGGAGT",
"BODY_SITE":"skin",
"Description":"human skin"}},
{"id":"Sample6", "metadata":{
"BarcodeSequence":"CTAACTACCAAT",
"LinkerPrimerSequence":"CATGCTGCCTCCCGTAGGAGT",
"BODY_SITE":"skin",
"Description":"human skin"}}
],
"matrix_type": "sparse",
"matrix_element_type": "int",
"shape": [5, 6],
"data":[[0,2,1],
[1,0,5],
[1,1,1],
[1,3,2],
[1,4,3],
[1,5,1],
[2,2,1],
[2,3,4],
[2,5,2],
[3,0,2],
[3,1,1],
[3,2,1],
[3,5,1],
[4,1,1],
[4,2,1]
]
}
"""
if __name__ == '__main__':
main()
|