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<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
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<HTML><HEAD><TITLE>NDB Atlas Entry for Structure PD0082</TITLE>
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<H1>NDB ID: PD0082 </H1>
<P>
<H2>Features </H2>B-DNA double helix, overhanging bases
<H2>Compound name </H2>CATABOLITE GENE ACTIVATOR PROTEIN/DNA COMPLEX
<H2>Sequence in asymmetric unit </H2>
<UL>
<LI>Chain A: A A A A A T G C G A T
<LI>Chain C: C T A G A T C G C A T T T T T
<LI>Chain E: ASP PRO THR LEU GLU TRP PHE LEU SER HIS CYS HIS ILE HIS LYS TYR
PRO SER LYS SER THR LEU ILE HIS GLN GLY GLU LYS ALA GLU THR LEU TYR TYR ILE
VAL LYS GLY SER VAL ALA VAL LEU ILE LYS ASP GLU GLU GLY LYS GLU MET ILE LEU
SER TYR LEU ASN GLN GLY ASP PHE ILE GLY GLU LEU GLY LEU PHE GLU GLU GLY GLN
GLU ARG SER ALA TRP VAL ARG ALA LYS THR ALA CYS GLU VAL ALA GLU ILE SER TYR
LYS LYS PHE ARG GLN LEU ILE GLN VAL ASN PRO ASP ILE LEU MET ARG LEU SER ALA
GLN MET ALA ARG ARG LEU GLN VAL THR SER GLU LYS VAL GLY ASN LEU ALA PHE LEU
ASP VAL THR GLY ARG ILE ALA GLN THR LEU LEU ASN LEU ALA LYS GLN PRO ASP ALA
MET THR HIS PRO ASP GLY MET GLN ILE LYS ILE THR ARG GLN GLU ILE GLY GLN ILE
VAL GLY CYS SER ARG GLU THR VAL GLY ARG ILE LEU LYS MET LEU GLU ASP GLN ASN
LEU ILE SER ALA HIS GLY LYS THR ILE VAL VAL TYR GLY </LI></UL>
<H2>Citation </H2>S. Chen, J. Vojtechovsky, G.N. Parkinson, R.H. Ebright, H.M.
Berman<BR>Indirect Readout of DNA Sequence at the Primary-kink Site in the
CAP-DNA Complex: DNA Binding Specificity Based on Energetics of DNA
Kinking<BR><I>J. Mol. Biol.,</I> <B>314</B>, pp. 63-74, 2001.
<P>
<H2>Space group </H2>P 3<SUB>1</SUB> 2 1
<H2>Cell constants </H2><PRE> A = 76.900 B = 76.900 C = 143.640 (ngstroms)
alpha = 90.00 beta = 90.00 gamma = 120.00 (degrees)
</PRE>
<H2>Crystallization conditions </H2>
<UL>
<LI>Method: vapor diffusion, hanging drop
<LI>Drop: 1,4-dioxane, MES, NaCl, MgCl<SUB>2</SUB>, CAMP, peg 8000
<LI>Reservoir: peg 8000 </LI></UL>
<H2>Refinement </H2>The structure was refined using the CNS 0.5 program. The R
value is 23.1 for 9519 reflections in the resolution range 20.0 to 3.0 with
Fobs > 0.0 sigma(Fobs).
<P>
<H2>Coordinates </H2>The <A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/coords/pdb-coord/pd0082.pdb">coordinates
for the asymmetric unit</A> of this structure and the <A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/coords/pdbs-coord/pd0082.pdbs">coordinates
with symmetry related strands </A>are stored in the NDB archive.
<P>
<P>
<H2>Views of PD0082 </H2><A
href="http://ndbserver.rutgers.edu/NDB/NDBATLAS/P/pd0082/pd0082_1_gif.html">A
view of PD0082 </A>
<P><PRE>
</PRE>
<HR>
<P align=center><I>1995-2001 The Nucleic Acid Database Project<BR>Rutgers, The
State University of New Jersey </I></P></BODY></HTML>
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